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World Journal of Stem Cells 2017年第10期9卷 179-186页

作者： Harumichi Itoh Shimpei Nishikawa Tomoya Haraguchi Yu Arikawa Shotaro Eto Masato Hiyama Toshie Iseri Yoshiki Itoh Munekazu Nakaichi Yusuke Sakai Kenji Tani Yasuho Taura Kazuhito Itamoto Department of Small Animal Clinical Science Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Surgery Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Radiology Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Pathology Joint Faculty of Veterinary Medicine Yamaguchi University

AIM To identify and characterize functionally distinct subpopulation of adipose-derived stem cells(ADSCs).METHODS ADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase(ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis.RESULTS Approximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis. Although significant difference was not seen in proliferation capacity, the adipogenic and osteogenic differentiation capacity was higher in ALDHHi subpopulations than in ALDHLo. Gene set enrichment analysis revealed that ribosome-related gene sets were enriched in the ALDHHi subpopulation. CONCLUSION High ALDH activity is a useful marker for identifying functionally different subpopulations in murine ADSCs. Additionally, we suggested the importance of ribosome for differentiation of ADSCs by gene set enrichment analysis.

关键词：脂肪质导出 stem/stromal 房间醛脱氢酶活动流动 cytometry subpopulation 核糖体

Assessing parameter sensitivity in a university campus COVID-19 model with vaccinations

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Infectious Disease Modelling 2023年第2期8卷 374-389页

作者： Meghan Rowan Childs Tony E.Wong Rochester Institute of Technology 1 Lomb Memorial DrRochesterNY14623USA

From the beginning of the COVID-19 pandemic,universities have experienced unique challenges due to their dual nature as a place of education and residence.Current research has explored non-pharmaceutical approaches to combating COVID-19,including representing in models different categories such as age groups.One key area not currently well represented in models is the effect of pharmaceutical preventative measures,specifically vaccinations,on COVID-19 spread on college campuses.There remain key questions on the sensitivity of COVID-19 infection rates on college campuses to potentially time-varying vaccine immunity.Here we introduce a compartment model that decomposes a campus population into constituent subpopulations and implements vaccinations with timevarying efficacy.We use this model to represent a campus population with both vaccinated and unvaccinated individuals,and we analyze this model using two metrics of interest:maximum isolation population and symptomatic infection.We demonstrate a decrease in symptomatic infections occurs for vaccinated individuals when the frequency of testing for unvaccinated individuals is increased.We find that the number of symptomatic infections is insensitive to the frequency of testing of the unvaccinated subpopulation once about 80%or more of the population is vaccinated.Through a Sobol’global sensitivity analysis,we characterize the sensitivity of modeled infection rates to these uncertain parameters.We find that in order to manage symptomatic infections and the maximum isolation population campuses must minimize contact between infected and uninfected individuals,promote high vaccine protection at the beginning of the semester,and minimize the number of individuals developing symptoms.

关键词： COVID-19 Vaccination subpopulation Modeling Sensitivity analysis University

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence （12-18 years） and the effect of varicocele

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Asian Journal of Andrology 2016年第6期18卷 824-830页

作者： Fernando Vasquez Caries Soler Patricia Camps Anthony Valverde Almudena Garcia-Molina Research Group on Reproductive and Sexual Health Health Sciences Division Universidad del Norte EO. Box 1569 Barranquilla Colombia Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain Technological Institute of Costa Rica Agronomy School Animal Reproduction Department San Carlos Campus 223-21001 San Carlos Costa Rica

This work evaluates sperm head morphometric characteristics in adolescents from 12 to 18 years of age, and the effect of varicocele. Volunteers between 150 and 224 months of age （mean 191, n = 87）, who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia. Morphometric analysis of sperm heads was performed with principal component （PC） and discriminant analysis, Combining seminal fluid and sperm parameters provided five PCs： two related to sperm morphometry, one to sperm motility, and two to seminal fluid components. Discriminant analysis on the morphometric results of varicocele and nonvaricocele groups did not provide a useful classification matrix. Of the semen-related PCs, the most explanatory （40%） was related to sperm motility. Two PCs, including sperm head elongation and size, were sufficient to evaluate sperm morphometric characteristics. Most of the morphometric variables were correlated with age, with an increase in size and decrease in the elongation of the sperm head. For head size, the entire sperm population could be divided into two morphometric subpopulations, SP1 and SP2, which did not change during adolescence. In general, for varicocele individuals, SP1 had larger and more elongated sperm heads than SP2, which had smaller and more elongated heads than in nonvaricocele men. In summary, sperm head morphometry assessed by CASA-Morph and multivariate cluster analysis provides a better comprehension of the ejaculate structure and possibly sperm function. Morphometric analysis provides much more information than data obtained from conventional semen analysis.

关键词： adolescence CASA-Morph system seminal quality sperm head morphometry spermiogram subpopulation

Sperm kinematic, head morphometric and kinetic- morphometric subpopulations in the blue fox （Alopex lagopus）

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Asian Journal of Andrology 2017年第2期19卷 154-159页

作者： Caries Soler Jesus Contell Lorena Bori Maria Sancho Almudena Garcia-Molina Anthony Valverde Jan Segarvall Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Spain Technological Institute of Costa Rica San Carlos Campus School of Agronomy 223-21001 Alajuela Costa Rica Finnish Fur Breeders Association ProFur 65101 Vaasa Finland

This work provides information on the blue fox ejaculated sperm quality needed for seminal dose calculations. Twenty semen samples, obtained by masturbation, were analyzed for kinematic and morphometric parameters by using CASA-Mot and CASA-Morph system and principal component （PC） analysis. For motility, eight kinematic parameters were evaluated, which were reduced to PC1, related to linear variables, and PC2, related to oscillatory movement. The whole population was divided into three independent subpopulations： SP1, fast cells with linear movement; SP2, slow cells and nonoscillatory motility; and SP3, medium speed cells and oscillatory movement. In almost all cases, the subpopulation distribution by animal was significantly different. Head morphology analysis generated four size and four shape parameters, which were reduced to PC 1, related to size, and PC2, related to shape of the cells. Three morphometric subpopulations existed. SPI： large oval cells; SP2： medium size elongated cells; and SP3. small and short cells. The subpopulation distribution differed between animals. Combining the kinematic and morphometric datasets produced PC1, related to morphometric parameters, and PC2, related to kinematics, which generated four sperm subpopulations - SPI.. high oscillatory motility, large and short heads; SP2; medium velocity with small and short heads; SP3. slow motion small and elongated cells; and SP4. high linear speed and large elongated cells. subpopulation distribution was different in all animals. The establishment of sperm subpopulations from kinematic, morphometric, and combined variables not only improves the well-defined fox semen characteristics and offers a good conceptual basis for fertility and sperm preservation techniques in this species, but also opens the door to use this approach in other species, included humans.

关键词： integration of motility and morphology principal component analysis sperm morphometry subpopulation

Single-cell analysis reveals the origins and intrahepatic development of liver-resident IFN-γ-producing γδ T cells

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Cellular & Molecular Immunology 2021年第4期18卷 954-968页

作者： Yuan Hu Keke Fang Yanan Wang Nan Lu Haoyu Sun Cai Zhang Institute of Immunopharmaceutical Sciences School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinanShandongChina Institute of Diagnostics School of MedicineCheeloo College of MedicineShandong UniversityJinanShandongChina Institute of Immunology School of Basic Medical SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhuiChina

γδ T cells are heterogeneous lymphocytes located in various tissues.However,a systematic and comprehensive understanding of the origins of γδ T cell heterogeneity and the extrathymic developmental pathway associated with liver γδ T cells remain largely unsolved.In this study,we performed single-cell RNA sequencing(scRNA-seq)to comprehensively catalog the heterogeneity of γδ T cells derived from murine liver and thymus samples.We revealed the developmental trajectory of γδ T cells and found that the liver contains γδ T cell precursors(pre-γδ T cells).The developmental potential of hepatic γδ T precursor cells was confirmed through in vitro coculture experiments and in vivo adoptive transfer experiments.The adoptive transfer of hematopoietic progenitor Lin^(-)Sca-1^(+)Mac-1^(+)(LSM)cells from fetal or adult liver samples to sublethally irradiated recipients resulted in the differentiation of liver LSM cells into pre-γδ T cells and interferon-gamma^(+)(IFN-γ^(+))but not interleukin-17a^(+)(IL-17a^(+))γδ T cells in the liver.Importantly,thymectomized mouse models showed that IFN-γ-producing γδ T cells could originate from liver LSM cells in a thymus-independent manner.These results suggested that liver hematopoietic progenitor LSM cells were able to differentiate into pre-γδ T cells and functionally mature γδ T cells,which implied that these cells are involved in a distinct developmental pathway independent of thymus-derived γδ T cells.

关键词： γδT cells liver extrathymic development subpopulation differentiation

Puma （Puma concolor） epididymal sperm morphometry

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Asian Journal of Andrology 2016年第6期18卷 879-881页

作者： Hernan Cucho Virgilio Alarcon Cesar Ordonez Enrique Ampuero Aydee Meza Caries Solere Zootechny School National University of San Atonio Abad del Cusco Postal code 921 Cusco Peru Functional Biology and Physical Anthropology Department University of Valencia C/. Dr Moliner 50 46100 Burjassot Valencia Spain Proiser R+D S.L. Scientific Park of the University of Valencia 46980 Paterna Spain

The Andean puma （Puma concolor） has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component （PC） analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.

关键词： principal component analysis puma sperm morphometry subpopulation wild animal

Morphometric comparison by the ISAS CASA-DNAf system of two techniques for the evaluation of DNA fragmentation in human spermatozoa

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Asian Journal of Andrology 2016年第6期18卷 835-839页

作者： Sara Sadeghi Almudena Garcia-Molina Ferran Celma Anthony Valverde Sogol Fereidounfar Caries Soler Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park Universitat de Valencia 46980 Paterna Valencia Spain Technological Institute of Costa Rica San Carlos Campus School of Agronomy 223-21001 Alajuela Costa Rica Department of Botany Kharazmi University Tehran Iran

DNA fragmentation has been shown to be one of the causes of male infertility, particularly related to repeated abortions, and different methods have been developed to analyze it. In the present study, two commercial kits based on the SCD technique （Halosperm~ and SDFA） were evaluated by the use of the DNA fragmentation module of the ISAS vl CASA system. Seven semen samples from volunteers were analyzed. To compare the results between techniques, the Kruskal-Wallis test was used. Data were used for calculation of Principal Components （two PCs were obtained）, and subsequent subpopulations were identified using the Halo, Halo/Core Ratio, and PC data. Results from both kits were significantly different （P 〈 0.001）. In each case, four subpopulations were obtained, independently of the classification method used. The distribution of subpopulations differed depending on the kit used. From the PC data, a discriminant analysis matrix was obtained and a good a posterioriclassification was obtained （97.1% for Halosperm and 96.6% for SDFA）. The present results are the first approach on morphometric evaluation of DNA fragmentation from the SCD technique. This approach could be used for the future definition of a classification matrix surpassing the current subjective evaluation of this important sperm factor.

关键词： DNA fragmentation Halosperm morphometry principal component analysis SDFA subpopulation

Analysis of the Distribution of Tulipa fosteriana and Tulipa ingens. The Importance of Protecting Wild Plants, in Particular Tulips All over the World

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American Journal of Plant Sciences 2023年第5期14卷 613-624页

作者： Shukrullozoda Roza Shukrullo Qizi Dekhkonov Davron Burxonovich Khaydarov Khislat Kudratovich Umurzakova Zebuniso Iskandarovna Olimjonova Sadoqat Gùlomjon Qizi Norimova Guljakhon Komiljon Qizi Department of Genetics and Biotechnology Samarkand State University Samarkand Uzbekistan Department of Biology Namangan State University Namangan Uzbekistan Department of Botany Samarkand State University Samarkand Uzbekistan

The assessment of the distribution and importance of the protection of tulip varieties around the world and separately rare, wild-growing ancestors of cultivated tulips, in particular Tulipa fosteriana and Tulipa ingens on the globe, including Central Asia, Uzbekistan and the Samarkand region, was described. A distribution map was introduced to visualize the data. Information about the morphological description of the studied species was also given.

关键词： Distribution Area Threat of Extinction Protection subpopulation “Hot Spot”

A developmental stage-dependent switch of the mechanisms for prostate epithelial maintenance

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Asian Journal of Andrology 2013年第1期15卷 85-86页

作者： Li Xin Department of Molecular and Cellular Biology Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA Department of Pathology and Immunology Baylor College of Medicine One Baylor Plaza Houston TX 77030 USA Dan L. Duncan Cancer Center Baylor College of Medicine One Baylor Plaza. Houston TX 77030 USA

Previous studies demonstrated that adult murine prostate basal and luminal cells are independently self-sustained, but prostate basal cells possess the potential to differentiate into multiple lineages upon induction by embryonic urogenital sinus mesenchyme. Nevertheless, it is unknown how prostate epithelia mature during the postnatal stage. Recently, Ousset et al.

关键词： MURINE PROSTATE STEM-CELLS UROGENITAL SINUS BASAL-CELLS CANCER subpopulation EXPRESSION DUCTS

Conditions for Homogeneity of subpopulations

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Communications in Mathematical Research 1998年第3期 85-88页

作者：李树有史宁中

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关键词： and Phrases Homogeneity subpopulation Contingency Table