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A facile,versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity,cell viability and proliferation

引用

Regenerative Biomaterials 2021年第3期8卷 141-155页

作者： Hongqing Chen Fei Fei Xinda Li Zhenguo Nie Dezhi Zhou Libiao Liu Jing Zhang Haitao Zhang Zhou Fei Tao Xu Department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China Department of Neurosurgery Central Theater General HospitalWuhan 430010China Department of Ophthalmology Xijing HospitalFourth Military Medical UniversityXi’an 710032China Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing Department of Mechanical EngineeringTsinghua UniversityBeijing 100084China Key Laboratory for Advanced Materials Processing Technology Ministry of EducationDepartment of Mechanical EngineeringTsinghua UniversityBeijing 100084China Department of Neurosurgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital Chengdu 610072China Department of Orthopedics Fourth medical center of PLA general hospitalBeijing 100048China East China Institute of Digital Medical Engineering Shangrao 334000China Department of Precision Medicine and Healthcare Tsinghua-Berkeley Shenzhen InstituteShenzhen 518055China

Both of the long-term fidelity and cell viability of three-dimensional(3D)-bioprinted constructs are essential to precise soft tissue ***,the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs,and bioinks containing excessive polymer are detrimental to cell ***,we obtained a facile hydrogel by introducing 1%aldehyde hyaluronic acid(AHA)and 0.375%N-carboxymethyl chitosan(CMC),two polysaccharides with strong water absorption and water retention capacity,into classic gelatin(GEL,5%)–alginate(ALG,1%)*** GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/*** fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at *** printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3%calcium chloride for only 20 *** cytometry results showed that the cell viability was 91.3861.55%on day 29,and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.0661.24%.This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

关键词： bioink bioprinting subaqueous fidelity cell viability

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