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Journal of Integrative Agriculture 2012年第4期11卷 585-592页

作者： WU Sha-sha WU Jin-di JIAO Xue-hui ZHANG Qi-xiang LV Ying-min College of Landscape Architecture Beijing Forestry University Beijing 100083 P.R.China China National Research Center for Floriculture Engineering ＆ Technology Beijing 100083 P.R.China

The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to *** contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis,indicating that these scales served as a source for growth and *** flowering,the number of starch granules and lipid droplets increased dramatically,and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb *** observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb *** activity of β-amylase,one of the key enzymes catalyzing starch breakdown,showed a similar *** enzyme sub-cellular localization via immune-gold electron-microscopy showed that βamylase was predominantly located together with starch granules,while the gold particles were scarcely found in other sub-cellular *** result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.

关键词： Lilium Oriental hybrids bulb scale source and sink ultra-structure β-amylase sub-cellular localization

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Expression and sub-cellular localization of leucine-rich repeats and immunoglobulin-like domains are related to antioxidant enzymes in human ependymoma and oligodendroglioma

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Neural Regeneration Research 2011年第11期6卷 860-864页

作者： Wei Yi Lin Liu Okechi Humphrey Qianxue Chen Shulan Huang Department of Neurosurgery Renmin Hospital of Wuhan University Wuhan 430060 Hubei Province China Department of Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 （LRIG1） and antioxidant enzymes and related proteins, including manganese superoxide dismutase, glutamate cysteine ligase catalytic or regulatory subunit, thioredoxin and thioredoxin reductase, in both human ependymoma and oligodendroglioma. Results revealed that the cytoplasmic expression of LRIG1 was associated with expression of glutamate cysteine ligase catalytic subunit in the human ependymoma, while the nuclear expression of LRIG1 was associated with expression of thioredoxin reductase. In human oligodendroglioma, the cytoplasmic expression of LRIG1 was associated with expression of the glutamate cysteine ligase catalytic subunit. Both the nuclear and perinuclear expressions of LRIG1 were associated with expression of glutamate cysteine ligase regulatory subunit. These results indicated that several antioxidant enzymes and related proteins contributed to LRIG1 expression, and that these may participate in the antioxidation of the cells.

关键词： leucine-rich repeats and immunoglobulin-like domain 1 sub-cellular localization ependymoma oligodendroglioma antioxidant enzymes

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Expression and sub-cellular localization of leucine-rich repeats and immunoglobulin-like domains is related to antioxidant enzymes in human ependymoma and oligodendroglioma

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Expression and sub-cellular localization of leucine-rich rep...

Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle

2011中华医学会神经外科学学术会议

作者： Wei Yi1 Lin Liu1 Qianxue Chen1 Shulan Huang1 1.Renmin Hospital of Wuhan University

The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 (LRIG1) and antioxidant enzymes and related proteins, including manganese superoxide dismutas... 详细信息

关键词： leucine-rich repeats and immunoglobulin-like domain 1 sub-cellular localization ependymoma oligodendroglioma antioxidant enzymes neural regeneration

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Cell Research 2005年第3期15卷 183-186页

作者： Aniruddha SENGUPTA,Bhaswati BANERJEE,Rakesh K. TYAGI,Kasturi DATTA Biochemistry Laboratory School of Environmental Sciences Jawaharlal Nehru University New Delhi-110067 India Biochemistry Laboratory School of Environmental Sciences Jawaharlal Nehru University New Delhi-110067 India Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi India Biochemistry Laboratory School of Environmental Sciences Jawaharlal Nehru University New Delhi-110067 India2Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi India

Hyaluronan binding protein 1 (HABP1) is a negatively charged multifunctional mammalian protein with a unique structural fold. Despite the fact that HABP1 possesses mitochondrial localization signal, it has also been localized to other cellular compartments. Using indirect immunofluorescence, we examined the sub-cellular localization of HABP1 and its dynamics during mitosis. We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell. Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis. This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan, suggesting that in concert with each other the two molecules play critical roles in this dynamic process.

关键词： Hyaluronan Binding Protein 1 (HABP1) hyaluronan sub-cellular localization golgi mitosis.

Isolation and Functional Analysis of the bZIP Transcription Factor Gene TaABP1 from a Chinese Wheat Landrace

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Journal of Integrative Agriculture 2012年第10期11卷 1580-1591页

作者： CAO Xin-you CHEN Ming XU Zhao-shi CHEN Yao-feng LI Lian-cheng YU Yue-hua LIU Yang-na MAYou-zhi National Key Facility for Crop Genetic Resources and Genetic Improvement Key Laboratory of Crop Genetics and BreedingMinistry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Crop Research Institute Shandong Academy of Agricultural Sciences College of Agronomy Northwest A&F University

In plants, basic leucine zipper （bZIP） transcription factors play important roles in regulatory processes, including stress response, pathogenic defense and light response as well as organ and tissue differentiation. Chinese wheat landrace Pingyaoxiaobaimai （PYXBM）, an original parent of drought tolerant wheat varieties grown in northern China, is significantly tolerant to abiotic stresses such as drought, cold and nutrient deficiencies. In order to isolate key stress-responsive genes and then improve stress tolerances of conventional varieties, a bZIP transcription factor gene was isolated from a cDNA library of drought-treated PYXBM using the in situ plaque hybridization method, and was designated as Triticum aestivum L. abscisic acid （ABA）-responsive element binding protein 1 （TaABP1）. It encodes 372 amino acids, and contains three conserved domains （C1-C3） in the N terminal and a bZIP domain in the C terminal which is a typical protein structure for the group member of bZIP family. Transcriptional activation analysis showed that TaABP1 activated the expression of downstream reporter genes in yeast without ABA application. TaABP1 protein fused with green fluorescent protein （GFP） demonstrated that the localization of TaABP 1 protein is in the nucleus. Expression pattern assays indicated that TaABP1 was strongly induced by ABA, high salt, low temperature and drought, and its expression was stronger in stems and leaves than in the roots of wheat. Furthermore, overexpression of TaABP1 in tobacco showed significant improvement of drought tolerance. Data suggested that TaABP1 may be a good candidate gene for improving stress tolerance of wheat by genetic transformation and elucidation of the role of this gene will be useful for understanding the mechanism underlying drought tolerance of Chinese wheat landrace PYXBM.

关键词： wheat expression pattern stress tolerance sub-cellular localization transcriptional activation

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