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Fine mapping and transcriptome sequencing reveal candidate genes conferring all-stage resistance to stripe rust on chromosome arm 1AL in Chinese wheat landrace AS1676

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作物学报:英文版 2023年第5期11卷 1501-1511页

作者： Xiu Yang Yunfeng Jiang Xianghai Yu Haipeng Zhang Yuqi Wang Fangnian Guan Li Long Hao Li Wei Li Qiantao Jiang Jirui Wang Yuming Wei Jian Ma Houyang Kang Pengfei Qi Qiang Xu Meng Deng Yazhou Zhang Youliang Zheng Yonghong Zhou Guoyue Chen State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China WenjiangChengdu 611130SichuanChina Triticeae Research Institute Sichuan Agricultural UniversityWenjiangChengdu 611130SichuanChina College of Agronomy Sichuan Agricultural UniversityWenjiangChengdu 611130SichuanChina

stripe rust, caused by Puccinia striiformis f. sp. tritici(Pst), threatens wheat production worldwide, and resistant varieties tend to become susceptible after a period of cultivation owing to the variation of pathogen races. In this study, a new resistance gene against Pst race CYR34 was identified and predicted using the descendants of a cross between AS1676, a highly resistant Chinese landrace, and Avocet S, a susceptible cultivar. From a heterozygous plant from a F7recombinant inbred line(RIL) population lacking the Yr18 gene, a near-isogenic line(NIL) population was developed to map the resistance gene. An allstage resistance gene, YrAS1676, was identified on chromosome arm 1AL via bulked-segregant exomecapture sequencing. By analyzing a large NIL population consisting of 6537 plants, the gene was further mapped to the marker interval between KA1A_485.36 and KA1A_490.13, spanning 485.36–490.13 Mb on1AL. A total of 66 annotated genes have been reported in this region. To characterize and predict the candidate gene(s), an RNA-seq was performed using NIL-R and NIL-S seedlings 3 days after CYR34 inoculation. Compared to NIL-S plants, NIL-R plants showed stronger immune reaction and higher expression levels of genes encoding pathogenesis-associated proteins. These differences may help to explain why NIL-R plants were more resistant to Pst race CYR34 than NIL-S plants. By combining fine-mapping and transcriptome sequencing, a calcium-dependent protein kinase gene was finally predicted as the potential candidate gene of YrAS1676. This gene contained a single-nucleotide polymorphism. The candidate gene was more highly expressed in NIL-R than in NIL-S plants. In field experiments with Pst challenge,the YrAS1676 genotype showed mitigation of disease damage and yield loss without adverse effects on tested agronomic traits. These results suggest that YrAS1676 has potential use in wheat stripe rust resistance breeding.

关键词： stripe rust All-stage resistance(ASR) BSE-Seq Transcriptome analyses Candidate genes

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Identification and Validation of a Major Quantitative Trait Locus for Slow-rusting Resistance to stripe rust in Wheat

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Journal of Integrative Plant Biology 2012年第5期54卷 330-344页

作者： Xiaohua Cao Jianghong Zhou Xiaoping Gong Guangyao Zhao Jizeng Jia Xiaoquan Qi Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Graduate University of Chinese Academy of Sciences Beijing 100049China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China These authors contributed equally to this article.

stripe （yellow） rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks （Pst）, is one of the most important wheat （Triticum aestivum L.） diseases and causes significant yield losses. A recombinant inbred （RI） population derived from a cross between Yanzhan 1 and Xichang 76-9 cultivars was evaluated for resistance to wheat stripe rust strain CYR32 at both the seedling and adult plant stages. Four resistance quantitative trait loci （QTLs） were detected in this population, in which the major one, designated as Yrql, was mapped on chromosome 2DS. The strategy of using the Brachypodium distachyon genome, wheat expressed sequence tags and a draft DNA sequences （scaffolds） of the D-genome （Aegilops tauschfi Coss.） for the development of simple sequence repeat （SSR） markers was successfully used to identify 147 SSRs in hexaploid wheat. Of the 19 polymorphic SSRs in the RI population, 17 SSRs were mapped in the homeologous group 2 chromosomes near Yrql region and eight SSRs were genetically mapped in the 2.7 cM region of Yrql, providing abundant DNA markers for fine-mapping of Yrql and marker-assisted selection in wheat breeding program. The effectiveness of Yrql was validated in an independent population, indicating that this resistance QTL can be successfully transferred into a susceptible cultivar for improvement of stripe rust resistance.

关键词： Quantitative trait locus slow-rusting stripe rust wheat.

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Identification of eight Berberis species from the Yunnan-Guizhou plateau as aecial hosts for Puccinia striiformis ***,the wheat stripe rust pathogen

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Journal of Integrative Agriculture 2021年第6期20卷 1563-1569页

作者： LI Si-nan CHEN Wen MA Xin-yao TIAN Xia-xia LIU Yao HUANG Li-li KANG Zhen-sheng ZHAO Jie State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityYangling 712100P.R.China Guizhou Institute of Plant Protection Guizhou Academy of Agricultural SciencesGuiyang 550006P.R.China

Puccinia striiformis *** Erikss.(Pst)infects wheat and causes stripe *** rust is heteroecious with wheat as the primary uredinial and telial host and barberry(Berberis spp.)as the alternate pycnial and aecial *** than 40 Berberis species have been identified as alternate hosts for Pst,and most of these are Chinese Berberis ***,little is known about Berberis species or their geographic distributions in the Yunnan-Guizhou plateau in southwestern *** Yunnan-Guizhou plateau is considered to be an important and relatively independent region for the evolution of the wheat stripe rust pathogen in China because the entire disease cycle can be completed within the *** this study,we conducted a survey of barberry plants in the Yunnan-Guizhou plateau and identified the eight Pst-susceptible Berberis species under controlled conditions,including ***,***,***,***,*** ***,***,*** and *** species are reported here for the first time to serve as alternate hosts for the wheat stripe rust pathogen under controlled conditions.

关键词： Berberis spp. alternate host Puccinia striiformis f.sp.tritici sexual reproduction stripe rust wheat yellow rust

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Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust

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Journal of Genetics and Genomics 2023年第11期50卷 872-882页

作者： Yan Yan Xiao-Ming Li Yun Chen Tian-Tian Wu Ci-Hang Ding Mei-Qi Zhang Yue-Ting Guo Chu-Yang Wang Junli Zhang Xuebin Zhang Awais Rasheed Shengchun Xu Meng-Lu Wang Zhongfu Ni Qixin Sun 缑金营 Frontiers Science Center for Molecular Design Breeding(MOE) China Agricultural UniversityBeijing 100193China Xianghu Laboratory HangzhouZhejiang 311231China School of Life Sciences Fudan UniversityShanghai 200438China Institute of Metabolism and Integrative Biology Fudan UniversityShanghai 200438China State Key Laboratory of Crop Stress Adaptation and Improvement Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityKaifengHenan 475004China International Maize and Wheat Improvement Center(CIMMYT) Chinese Academy of Agricultural SciencesBeijing 100081China

Wheat(Triticum aestivum)is one of the most essential human energy and protein ***,wheat production is threatened by devastating fungal diseases such as stripe rust,caused by Puccinia striiformis ***(Pst).Here,we reveal that the alternations in chloroplast lipid profiles and the accumulation of jasmonate(JA)in the necrosis region activate JA signaling and trigger the host *** collapse of chloroplasts in the necrosis region results in accumulations of polyunsaturated membrane lipids and the lipid-derived phytohormone JA in transgenic lines of Yr36 that encodes Wheat Kinase START 1(WKS1),a high-temperature-dependent adult plant resistance ***1.1,a protein encoded by a full-length splicing variant of WKS1,phosphorylates and enhances the activity of keto-acyl thiolase(KAT-2B),a critical enzyme catalyzing theβ-oxidation reaction in JA *** premature stop mutant,kat-2b,accumulates less JA and shows defects in the host defense against ***,overexpression of KAT-2B results in a higher level of JA and limits the growth of ***,JA inhibits the growth and reduces pustule densities of *** study illustrates the WKS1.1-KAT-2B-JA pathway for enhancing wheat defense against fungal pathogens to attenuate yield loss.

关键词： Wheat stripe rust Cell death Jasmonate Hostdefense

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Distribution,Frequency and Variation of stripe rust Resistance Loci Yr10,Lr34/Yr18 and Yr36 in Chinese Wheat Cultivars

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Journal of Genetics and Genomics 2012年第11期39卷 587-592页

作者： Cuiling Yuan Hui Jiang Honggang Wang Kun Li Heng Tang Xianbin Li Daolin Fu State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyShandong Agricultural University61 Daizong StreetTai'anShandong 271018China

Wheat stripe rust is a devastating disease in many regions of the world. In wheat, 49 resistance genes for stripe rust have been officially documented, but only three genes are cloned, including the race-specific resistance YrlO candidate gene （YrlOca） and slow-rusting genes Lr34/Yr18 （hereafter designated as Yrl8） and Fr36. In this study, we developed gene-specific markers for these genes and used them to screen a collection of 659 wheat accessions, including 485 Chinese cultivars. Thirteen percent and eleven percent of the tested Chinese cultivars were positive for the markers for YrlOca and Yr18nn （the resistant haplotype of YrlS）, respectively, but none were positive for the Yr36 marker. Since there is a limited use of the YrlO gene in Chinese wheat, the relatively high frequency of wheat varieties with the YrlOcG marker suggests that the identity of the YrlO gene is unknown. With regards to the Yr18 gene, 29% of the tested cultivars that are used in the Middle and Lower Yangtze Valleys＇ winter wheat zone were positive for Yrl8RH markers. A non-functional allele of Yrl8Rn was identified in ＇Mingxian 169＇, a commonly used susceptible check for studying stripe rust. The data presented here will provide useful information for marker-assisted selection for wheat stripe rust resistance.

关键词： Puccinia striiformis stripe rust Triticum aestivum

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Molecular Screening and Resistance Evaluation of American Wheat Cultivars to Chinese stripe rust Races

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Agricultural Sciences in China 2010年第10期9卷 1455-1461页

作者： BAI Yu-lu ZHANG Chun-yu SUN Quan LIN Feng CUI Na XU Shi-chang GAO Yang XUXiao-dan Bioscience and Biotechnology College Shenyang Agricultural University Shenyang 110161 P.R.China Plant Protection Institute Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China The State Key Laboratory for Biology of Plant Diseases and lnsect Pests Beijing 100094 P.R.China

stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the major diseases of wheat in China. In order to asses the resistance levels and existing Yr genes among 59 wheat cultivars （lines） from the Pacific Northwest （PNW） of the United States, to provide resistance resources for genetic improvement of wheat stripe rust resistance in China, 59 wheat cultivars （lines） from PNW of the United States were infected by 3 mixed races of predominant Chinese stripe rust races CRY31, CRY32, and CRY33 to evaluate their resistance at seedling and adult plant stages, and screened with molecular markers tightly linked to currently effective all-stage resistance genes YrlO, Yrl5 and adult plant resistance genes Yrl8, Yr39. Of 59 American cultivars （lines）, five cultivars （lines）, Expresso, 02W50076, ACS52610, WA008012, and WA00801833, had all-stage resistance, showing resistance to mixed races of CRY31, CRY32, and CRY33 at both seedling and adult plant stages. 33 cultivars （lines） had adult plant resistance, only showing resistance to stripe rust at adult stage. Based on the molecular screening, none of the 59 PNW cultivars （lines） had the polymorphic bands of linked markers to YrlO. There were 12, 33 and 29 cultivars （lines） which bad polymorphic bands of linked markers to Yr15, Yr18 and Yr39, accounting for 20, 55 and 49% of the 59 PNW cultivars （lines）, respectively. All these results suggested that Yr15, Yr18 and Yr39 were widespread among PNW cultivars （cultivars） and could be utilized in Chinese wheat stripe rust resistance breeding.

关键词： American wheat cultivar stripe rust molecular marker resistance evaluation

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Genetic Analysis and Molecular Mapping of an All-Stage stripe rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

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Journal of Integrative Agriculture 2013年第12期12卷 2197-2208页

作者： HOU Lu MA Dong-fang HU Mao-lin HE Miao-miao LU Yan JING Jin-xue State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University Qinghai Academy of Agriculture and Forestry Science Department of Plant Pathology China Agricultural University/Key Laboratory of Plant PathologyMinistry of Agriculture

Triticum aestivum-Hayaldia villosa translocation line V3 has shown effective all-stage resistance to the seven dominant pathotypes of Puccinia striiforms *** prevalent in *** elucidate the genetic basis of the resistance,the segregating populations were developed from the cross between V3 and susceptible genotype Mingxian 169,seedlings of the parents and F 2 progeny were tested with six prevalent pathotypes,including CYR29,CYR31,CYR32-6,CYR33,Sun11-4,and Sun11-11,F 1 plants and F 3 lines were also inoculated with Sun11-11 to confirm the result *** genetic studied results showed that the resistance of V3 against CYR29 was conferred by two dominant genes,independently,one dominant gene and one recessive gene conferring independently or a single dominant gene to confer resistance to CYR31,two complementary dominant genes conferring resistance to both CYR32-6 and Sun11-4,two independently dominant genes or three dominant genes（two of the genes show cumulative effect） conferring resistance to CYR33,a single dominant gene for resistance to *** gene analog polymorphism（RGAP） and simple-sequence repeat（SSR） techniques were used to identify molecular markers linked to the single dominant gene（temporarily designated as YrV3） for resistance to Sun11-11.A linkage map of 2 RGAP and 7 SSR markers was constructed for the dominant gene using data from 221 F 2 plants and their derived F 2：3 lines tested with Sun11-11 in the *** of the complete set of nulli-tetrasomic lines of Chinese Spring with a RGAP marker RG1 mapped the gene on the chromosome 1B,and then the linked 7 SSR markers located this gene on the long arm of chromosome *** linkage map spanned a genetic distance of 25.0 cM,the SSR markers Xgwm124 and Xcfa2147 closely linked to YrV3 with genetic distances of 3.0 and 3.8 cM,*** on the linkage map,it concluded that the resistance gene YrV3 was located on chromosome arm *** chromosomal location,

关键词： Haynaldia villosa translocation line stripe rust RGAP-SSR molecular mapping

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Genes for resistance to stripe rust on chromosome 2B and their application in wheat breeding

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Progress in Natural Science:Materials International 2009年第1期19卷 9-15页

作者： Peigao Luo a,b,Xueyun Hu a,Huaiyu Zhang a,Zhenglong Ren a,b,a State Key Laboratory of Plant Breeding and Genetics,Sichuan Agriculture University,Ya’an,Sichuan 625014,China b School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,China State Key Laboratory of Plant Breeding and Genetics Sichuan Agriculture University Ya’an Sichuan 625014 China School of Life Science and Technology University of Electronic Science and Technology of China Chengdu 610054 China

stripe rust,caused by Puccinia striiformis ***,is one of the most damaging diseases of wheat *** resistant cultivars is the most economic and environmental friendly way to control the *** are many resistance genes to stripe rust located on wheat chromosome ***,we propose a strategy to construct the recombinant wheat chromosome 2B with multiple resistances to stripe rust by making crosses between wheat lines or cultivars carrying Yr genes and using marker-assisted selection,based on the reported information about resistance spectrum,chromosomal location,and linked markers of the *** the resistance genes on 2B would afford a valuable strategy to control the disease by cultivating varieties with durable *** possibility,efficiency,and prospect of the suggested strategy are reviewed in the paper.

关键词： Puccinia striiformis Yr gene 2B chromosome stripe rust Marker-assisted selection

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Genomic analysis,trajectory tracking,and field surveys reveal sources and long-distance dispersal routes of wheat stripe rust pathogen in China

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Plant Communications 2023年第4期4卷 174-188页

作者： Yuxiang Li Jichen Dai Taixue Zhang Baotong Wang Siyue Zhang Conghao Wang Jiguang Zhang Qiang Yao Mingju Li Chengyun Li Yuelin Peng Shiqin Cao Gangming Zhan Fei Tao Haifeng Gao Weili Huang Xiaojun Feng Yingwen Bai Zhuoma Qucuo Hongsheng Shang Chong Huang Wancai Liu Jiasui Zhan Xiangming Xu Xianming Chen Zhensheng Kang Xiaoping Hu State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F UniversityTaicheng Road 3YanglingShaanxiChina KeyLaboratory of Agricultural Integrated PestManagement Qinghai ProvinceAcademyof Agriculture and ForestryScienceQinghai UniversityXiningQinghaiChina Yunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests Agricultural Environment and Resource InstituteYunnan Academy of Agricultural SciencesKunmingYunnanChina State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan Yunnan Agricultural UniversityKunmingYunnanChina Department of Plant Pathology Tibet Agricultural and Animal Husbandry CollegeLinzhiTibetChina Wheat Research Institute Gansu Academy of Agricultural SciencesLanzhouGansuChina Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province College of Plant ProtectionGansu Agricultural UniversityLanzhouGansuChina Institute of Plant Protection Xinjiang Academy of Agricultural SciencesUrumqiXinjiangChina Xi’an Huang’s Bio-technology Company Ltd Xi’anShaanxiChina Shaanxi Plant Protection Extension Station Xi’anShaanxiChina Baoji Plant Protection Extension Station BaojiShaanxiChina National Agricultural Technology Extension and Service Center Ministry of AgricultureBeijingChina Department of Forest Mycology and Plant Pathology Swedish University of Agricultural SciencesUppsalaSweden Pest&Pathogen Ecology NIAB EMREast MallingWest MallingKentUK Agricultural Research Service United States Department of Agriculture and Department of Plant PathologyWashington State UniversityPullmanWAUSA

Identifying sources of phytopathogen inoculum and determining their contributions to disease outbreaks are essential for predicting disease development and establishing control *** striiformis ***(Pst),the causal agent of wheat stripe rust,is an airborne fungal pathogen with rapid virulence variation that threatens wheat production through its long-distance *** of wide variation in geographic features,climatic conditions,and wheat production systems,Pst sources and related dispersal routes in China are largely *** the present study,we performed genomic analyses of 154 Pst isolates from all major wheat-growing regions in China to determine Pst population structure and *** trajectory tracking,historical migration studies,genetic introgression analyses,and field surveys,we investigated Pst sources and their contributions to wheat stripe rust *** identified Longnan,the Himalayan region,and the Guizhou Plateau,which contain the highest population genetic diversities,as the Pst sources in *** from Longnan disseminates mainly to eastern Liupan Mountain,the Sichuan Basin,and eastern Qinghai;that from the Himalayan region spreads mainly to the Sichuan Basin and eastern Qinghai;and that from the Guizhou Plateau migrates mainly to the Sichuan Basin and the Central *** findings improve our current understanding of wheat stripe rust epidemics in China and emphasize the need for managing stripe rust on a national scale.

关键词： Puccinia striiformis f.sp.tritici stripe rust disease epidemics population genetics genome sequencing

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Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat

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Plant Communications 2023年第5期4卷 295-308页

作者： Chao-Yan Chang Shu-Xian Yang Mei-Qi Zhang Yue-Ting Guo Xiao-Ming Li Yan Yan Ci-Hang Ding Ke-Xin Niu Meng-Lu Wang Qin-Quan Li Junli Zhang Xuebin Zhang Shisheng Chen Chaojie Xie Zhongfu Ni Qixin Sun Jin-Ying Gou Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China School of Life Sciences Fudan UniversityShanghai 200438China State Key Laboratory of Crop Stress Adaptation and Improvement Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityJinming RoadKaifeng 475004China Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences at WeifangWeifangShandong 261000China

Reducing losses caused by pathogens is an effective strategy for stabilizing crop *** challenges remain in cloning and characterizing genes that inhibit stripe rust,a devastating disease of wheat(Triticum aestivum)caused by Puccinia striiformis ***(Pst).We found that suppression of wheat zeaxanthin epoxidase 1(ZEP1)increased wheat defense against *** isolated the yellow rust slower 1(yrs1)mutant of tetraploid wheat in which a premature stop mutation in ZEP1-B underpins the *** analyses revealed increased H_(2)O_(2) accumulation in zep1 mutants and demonstrated a correlation between ZEP1 dysfunction and slower Pst growth in ***,wheat kinase START 1.1(WKS1.1,Yr36)bound,phosphorylated,and suppressed the biochemical activity of ZEP1.A rare natural allele in the hexaploid wheat ZEP1-B promoter reduced its transcription and Pst *** study thus identified a novel suppressor of Pst,characterized its mechanism of action,and revealed beneficial variants for wheat disease *** work opens the door to stacking wheat ZEP1 variants with other known Pst resistance genes in future breeding programs to enhance wheat tolerance to pathogens.

关键词： wheat stripe rust reactive oxygen species zeaxanthin epoxidase

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