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Infectious Diseases of Poverty 2017年第1期6卷 1379-1391页

作者： Paul L.A.M.Corstjens Pytsje T.Hoekstra Claudia J.de Dood Govert J.van Dam Department of Molecular Cell Biology Leiden University Medical CenterP.O.Box 96002300RCLeidenThe Netherlands Department of Parasitology Leiden University Medical CenterP.O.Box 96002300RCLeidenThe Netherlands

Background:Methodological applications of the high sensitivity genus-specific Schistosoma CAA strip test,allowing detection of single worm active infections(ultimate sensitivity),are discussed for efficient utilization in sample pooling *** relevant cost reduction,pooling of samples rather than individual testing can provide valuable data for large scale mapping,surveillance,and ***:The laboratory-based CAA strip test utilizes luminescent quantitative up-converting phosphor(UCP)reporter particles and a rapid user-friendly lateral flow(LF)assay *** test includes a sample preparation step that permits virtually unlimited sample concentration with urine,reaching ultimate sensitivity(single worm detection)at 100%*** facilitates testing large urine pools from many individuals with minimal loss of sensitivity and *** test determines the average CAA level of the individuals in the pool thus indicating overall worm burden and *** requiring test results at the individual level,smaller pools need to be analysed with the pool-size based on expected prevalence or when unknown,on the average CAA level of a larger group;CAA negative pools do not require individual test results and thus reduce the number of ***:Straightforward pooling strategies indicate that at sub-population level the CAA strip test is an efficient assay for general mapping,identification of hotspots,determination of stratified infection levels,and accurate monitoring of mass drug administrations(MDA).At the individual level,the number of tests can be reduced *** low endemic settings as the pool size can be increased as opposed to prevalence ***:At the sub-population level,average CAA concentrations determined in urine pools can be an appropriate measure indicating worm *** strategies allowing this type of large scale testing are feasible with the various CAA strip test formats and do not affect sens

关键词： Schistosomiasis Diagnostics Sample pooling Mass drug administration Circulating anodic antigen Lateral flow strip test Up-converting reporter particle UCP-LF CAA

Distinguishing of Abuse Drugs in Urine and Blood Samples of Abusers in Iran

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Journal of Pharmacy and Pharmacology 2015年第8期3卷 385-390页

作者： Rezaei-Basiri Majid Behshid Mojgan Ghazi-Khansari Mahmoud Tabatabaei Seyed Mahmoud Moradi Danesh Alilu Saleh Jabbarzadeh Aahad Mokhtari Marziyeh Bahari Afrouz Department of Pharmacology and Toxicology School of Pharmacy Tabriz University of Medical Sciences Tabriz 51999 Iran Medical Education Research Center Tabriz University of Medical Sciences Tabriz 51999 Iran Department of Medical Surgical School of Nursing and Midwifery Tabriz University of Medical Sciences Tabriz 51999 Iran Department of Pharmacology and Toxicology School of Medicine Tehran University of Medical Sciences Tehran 13111 Iran Department of Physiology Tabriz Branch lslamic Azad University Tabriz 51999 Iran Welfare Organization of East Azarbayjan Tabriz 51999 Iran

In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples referred who addicts in clinics of Welfare Organization, during of detoxification treatment or maintenance treatment were screened for abuse drugs presence. Age ranges of female patients were 35 ± 15 and age range of males patients were 42 ± 18. All patients filled questionnaire and satisfy forms too. All data were analyzed by t-test and were Anowa one way, and P 〈 0.05 was considered significant. The P value of this study was P = 0.000. In this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis and TLC （thin-layer chromatography） is appropriate confirmation method to drug abuse distinguishing. Also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse.

关键词： Abusers urine and blood samples strip test Thin-layer chromatography abuse drugs.

Evaluation of Abuse Drugs and Clinical Laboratory tests Variations in Whole Blood ＆ Urine Samples of Abusers

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Journal of Pharmacy and Pharmacology 2018年第1期6卷 69-76页

作者： Majid Rezaei Basiri Mojgan Behshid Alireza Najafi Saleh Alilou Haniyeh Mohebbi-Kamali: Fatemeh Hosseindoust Marziyeh Mokhtari Valiyollah Watani Ladan Aminzadeh Fatemeh Seyed Nejad Ashraf Razavi Ligha Saadat Department of Pharmacology and Toxicology School of Pharmacy Tabriz University of Medical Sciences Tabriz 51999 Iran Medical Education Research Center Tabriz University of Medical Sciences Tabriz 51999 Iran Department of Medical Surgical School of Nursing and Midwifery Tabriz University of Medical Sciences Tabriz 51999 Iran Forensic and Legal Medicine of Organization Tabriz 515 7733541 Iran Welfare Organization of East Azarbayjan Tabriz 5156935 761 Iran

In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples of addicts in clinics of welfare organization, during detoxification treatment or maintenance treatment were screened for abuse drugs presence. The all of samples were tested through as a view of clinical laboratory methods. Age ranges of female patients were 35 ~ 15 and age range of males patients were 45 ~ 15. All patients filled questionnaire and satisfy forms too. First, all fresh urine and blood samples were examined to confirm presence drugs abuses, depend on their addiction and treatment, so all samples were confirmed by two tests. Then they were examined to other clinical laboratory tests. All data were analyzed by t-test and were Anova one way and two ways of Anova Turkey, and p 〈 0.05 was considered significant. The p-value of this study was p = 0.0001. The results of this study were showed that 4% of abusers had mild increase in hematocrite level and 2% of narcotic drugs abusers had mild lower level of blood sugars than normal range and 4% of participants had increase liver enzymes such ALT （alanine transferase）, AST （aspartat transferase）, ALP （alkaline phosphatease） and 1% of them had renal failure. Although blood level BUN （blood urea nitrogen） and creatinin were examined to evaluation of their renal failure .The results in Tabriz/Iran undrevision of welfare organization clinics were approximately showed that positive results of addiction are in each of urine and blood samples. Because some of abusers directly consumed full long time agonist or partial agonists＇ drugs such as methadone and buprenorphine for their maintenance therapy in clinics. Also doing test on blood samples has high importance in distinguishing and confirmation of drugs abuse in samples. Also in this study we conclude that among all drug analytical methods the cheapest

关键词： Abusers urine and whole blood samples strip test thin-layer chromatography abuse drugs clinical laboratory tests.

Fluorescence strip sensor for sensitive determination of prometryn in foods

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One Health Advances 2023年第1期1卷 268-275页

作者： Weifang Gu Steven Suryoprabowo Lingling Guo Aihong Wu Liqiang Liu Chuanlai Xu Affiliated Hospital of Jiangnan University Wuxi 214122China State Key Lab of Food Science and Technology School of Food Science and TechnologyJiangnan UniversityWuxiJiangsu 214122China

We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−***,the cut-off value for use in an LFIA was 50 ng mL−*** validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.

关键词： Fluorescence immunochromatographic test Monoclonal antibody Prometryn strip test