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Science China(Life Sciences) 2020年第2期63卷 251-258页

作者： Muhammad Ramzan Manwar Changjun Shao Xing Shi Jian Wang Qiang Lin Yigang Tong Yu Kang Jun Yu CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100190China Beijing Institute of Microbiology and Epidemiology Beijing 100071China

RtcB, a highly conserved RNA ligase, is found in all three domains of life, and demonstrated to be an essential tRNA splicing component in archaea and metazoans. However, the biological functions of RtcB in bacteria, where there is no splicing, remains to be clarified. We first performed bioinformatics analysis which revealed highly conserved structures and presumably conserved functions of RtcB in bacteria. However, its orthologs only occur in ~0.5% of bacterial species across diverse phyla with significant signals of frequent horizontal transfer, highlighting its non-essential role in bacteria. Next, by constructing an rtcBknockout strain, we find that the removal of antibiotic stress induces a significant impact on rtcB expression in wild-type strain,and furthermore the depletion of RtcB(?RtcB strain) delays the recovery process. Our transcriptomic analysis, comprising the3′-end labeling of RNAs, highlights a significant increase in unmapped reads and cleaved rRNAs in the ?RtcB strain, particularly during recovery. Our observations suggest that the conserved RNA ligase RtcB, repairs damaged r RNAs following stress,which potentially saves energy and accelerates recovery of its host. We propose that acquisition of RtcB by diverse bacterial taxa provides a competitive advantage under stressful conditions.

关键词： RtcB bacteria RNA ligase stress response ribosomal RNA RNA repair

Blockade of c-Src Within the Paraventricular Nucleus Attenuates Inflammatory Cytokines and Oxidative stress in the Mechanism of the TLR4 Signal Pathway in Salt-Induced Hypertension

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Neuroscience Bulletin 2020年第4期36卷 385-395页

作者： Qing Yang Xiao-Jing Yu Qing Su Qiu-Yue Yi Xin-Ai Song Xiao-Lian Shi Hong-Bao Li Jie Qi Guo-Qing Zhu Yu-Ming Kang Department of Physiology and Pathophysiology Xi'an Jiaotong University School of Basic Medical SciencesKey Laboratory of Environment and Genes Related to Diseases of the Ministry of EducationXi'an Jiaotong UniversityXi’an 710061China Department of Cardiovascular Surgery First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Department of Pharmacology School of Basic Medical SciencesXi’an Jiaotong University Health Science CenterXi’an 710061China Key Laboratory of Cardiovascular Disease and Molecular Intervention Department of PhysiologyNanjing Medical UniversityNanjing 210029China

Toll-like receptor 4 (TLR4) and cellular Src (cSrc) are closely associated with inflammatory cytokines and oxidative stress in hypertension, so we designed this study to explore the exact role of c-Src in the mechanism of action of the TLR4 signaling pathway in salt-induced hypertension. Salt-sensitive rats were given a high salt diet for 10 weeks to induce hypertension. This resulted in higher levels of TLR4, activated c-Src, pro-inflammatory cytokines, oxidative stress, and arterial pressure. Infusion of a TLR4 blocker into the hypothalamic paraventricular nucleus (PVN) decreased the activated c-Src, while microinjection of a c-Src inhibitor attenuated the PVN levels of nuclear factor-kappa B, pro-inflammatory cytokines, and oxidative stress. Our findings suggest that a longterm high-salt diet increases TLR4 expression in the PVN and this promotes the activation of c-Src, which upregulates the expression of pro-inflammatory cytokines and results in the overproduction of reactive oxygen species.Therefore, inhibiting central c-Src activity may be a new target for treating hypertension.

关键词： Toll-like receptor 4 Cellular Src Inflammatory cytokines Oxidative stress Hypothalamic paraventricular nucleus Salt-induced hypertension

Monitoring the effects of land transformation on African clawless otters(Aonyx capensis)using fecal glucocorticoid metabolite concentrations as a measure of stress

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Integrative Zoology 2020年第4期15卷 293-306页

作者： Tshepiso L.MAJELANTLE Trevor MCINTYRE Andre GANSWINDT Mammal Research Institute Department of Zoology and EntomologyUniversity of PretoriaPretoriaSouth Africa Department of Life and Consumer Sciences University of South AfricaPretoriaSouth Africa Endocrine Research Laboratory Department of Anatomy and PhysiologyUniversity of PretoriaOnderstepoortSouth Africa Centre for Veterinary Wildlife Studies Faculty of Veterinary ScienceUniversity of PretoriaOnderstepoortSouth Africa

In a time of increasing environmental change caused by anthropogenic disturbance,there is a greater need to understand animal adaptations to manmade environments.In this regard,the measurement of stress-related endocrine markers provides a useful tool to examine the impact of environmental challenges and the physiological consequences for wildlife occupying such space.The aims of the present study were to validate fecal glucocorticoid metabolite(fGCM)concentrations as a measure of stress using samples from a male African clawless otter(Aonyx capensis;n=1)and to compare fGCM concentrations of otters occurring in a transformed and in 2 natural areas in South Africa.From the 5 different enzyme-immunoassays(EIA)tested,a cortisol and oxoetiocholanolone(measuring 11,17 dioxoandrostanes)EIA revealed the highest response(74%and 48%increase,respectively)30 and 24 hours after a stress event(translocation of a captive individual as part of its rehabilitation prior to release),respectively.For both EIAs,fGCM concentrations were comparable for samples collected up to 3 h post-defecation.Using the cortisol EIA for subsequent analyses,fGCM concentrations of animals from the transformed area(n=20;mean[±SD]:0.468[±0.539]µg/g dry weight[DW])were significantly higher(P=0.013)than those from otters in the natural areas(n=17;0.242[±0.226]µg/g DW).These preliminary results suggest that African clawless otters may have increased adrenocortical activity that could be due to conditions linked to living in a transformed environment.

关键词： African clawless otter fecal glucocorticoid metabolites non-invasive hormone measurement stress urbanization

Effect of fluid shear stress on the internalization of kidney-targeted delivery systems in renal tubular epithelial cells

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Acta Pharmaceutica Sinica B 2020年第4期10卷 680-692页

作者： Yingying Xu Shuo Qin Yining Niu Tao Gong Zhirong Zhang Yao Fu Key Laboratory of Drug-Targeting and Drug Delivery Systems of Ministry of Education and Sichuan Province Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610041China

Renal tubular epithelial cells(RTECs)are important target cells for the development of kidney-targeted drug delivery systems.Under physiological conditions,RTECs are under constant fluid shear stress(FSS)from original urine in the renal tubule and respond to changes of FSS by altering their morphology and receptor expression patterns,which may affect reabsorption and cellular uptake.Using a microfluidic system,controlled shear stress was applied to proximal tubule epithelial cell line HK-2.Next,2-glucosamine,bovine serum albumin,and albumin nanoparticles were selected as representative carriers to perform cell uptake studies in HK-2 cells using the microfluidic platform system with controlled FSS.FSS is proven to impact the morphology of HK-2 cells and upregulate the levels of megalin and clathrin,which then led to enhanced cellular uptake efficiencies of energy-driven carrier systems such as macromolecular and albumin nanoparticles in HK-2 cells.To further investigate the effects of FSS on endocytic behavior mediated by related receptors,a mice model of acute kidney injury with reduced fluid shear stress was established.Consistent with in vitro findings,in vivo studies have also shown reduced fluid shear stress down-regulated the levels of megalin receptors,thereby reducing the renal distribution of albumin nanoparticles.

关键词： Fluid shear stress Kidney-targeted drug delivery HK-2 cells Acute kidney injury

Histone Deacetylase HDA9 and WRKY53 Transcription Factor Are Mutual Antagonists in Regulation of Plant stress Response

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Molecular Plant 2020年第4期13卷 598-611页

作者： Yu Zheng Jingyu Ge Chun Bao Wenwen Chang Jingjing Liu Jingjie Shao Xiaoyun Liu Lufang Su Lei Pan Dao-Xiu Zhou Institute for Interdisciplinary Research and Hubei Province Engineering Research Center of Legume Plants Jianghan UniversityWuhan 430056China Institute of Plant Sciences Paris-Saclay CNRSINRAEUniversite Paris-SaclayOrsay 91405France

Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant response to stress in Arabidopsis.However,how HDA9 targets to specific chromatin loci and controls gene expression netw orks involved in plant response to stress remains largely unclear.Here,we show that HDA9 represses stress tolerance response by interacting with and regulating the DNA binding and transcriptional activity of WRKY53,which functions as a high-hierarchy positive regulator of stress response.We found that WRKY53 is post-translationally modified by lysine acetylation at multiple sites,some of which are removed by HDA9,resulting in inhibition of WRKY53 transcription activity.Conversely,WRKY53 negatively regulates HDA9 histone deacetylase activity.Collectively,our results indicate that HDA9 and WRK53 are reciprocal negative regulators of each other's activities,illustrating how the functional interplay between a chromatin regulator and a transcription factor regulates stress tolerance in plants.

关键词： HDA9 WRKY53 stress lysine acetylation transcription activity

Prevalence and Factors Associated with Depression, Anxiety and stress in IBD Patients Undergoing Intravenous Biological Therapy during the COVID-19 Pandemic-Montenegro Experience

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International Journal of Mental Health Promotion 2022年第4期24卷 551-564页

作者： Brigita Smolović MarijaĐurović MilošLukić Marija Abramović Damir Muhović Department of Gastroenterohepatology Clinic for Internal MedicineClinical Center of MontenegroPodgorica81000Montenegro Faculty of Medicine University of MontenegroPodgorica81000Montenegro Clinic of Radiology Clinical Center of MontenegroPodgorica81000Montenegro

Throughout its duration,the coronavirus disease 2019(COVID-19)pandemic has been affecting lives worldwide and has had a sizeable impact on mental health,particularly for those who already suffer from a chronic illnesses.Depression,Anxiety and stress(DAS)are common psychiatric comorbidities in inﬂammatory bowel disease(IBD)patients.This study aims to determine the prevalence and risk factors for moderate and severe symptoms of DAS in IBD patients have been undergoing intravenous biological therapy(IvBTh)during the COVID-19 pan-demic.The study was conducted between September 1st and November 30th,2020 at the Clinical Center of Mon-tenegro-IBD unit,where all patients from Montenegro received the registered IvBTh.This case control study consists of 94 IBD patients that completed a validated questionnaire Depression,Anxiety and stress Score-21(DASS-21).A total of 59 patients received tumor necrosis factor alpha inhibitors(anti-TNF therapy),while 35 received anti-integrin therapy.After we calculated the DASS-21 score,we divided the patients into two groups:those who had moderate and severe symptoms(if they had any of the mentioned DASS-21 subscale score above limit for moderate or more severe symptoms:DASS-21 Depression,higher than 14;DASS-21 Anxiety,higher than 10 and DASS-21 stress,higher than 19)and those who did not have signiﬁcant symptoms(DASS-21 subscale score did not exceed the limit).We also examined demographic data,data on IBD characteristics and COVID-19 data and their impact on mental disorders.Standard statistical processing tests were used to identify risk factors for examined mental disorders.Following the DASS-21 criteria,we diagnosed the least moderate depression in 19.1%,anxiety in 14.9%and stress in 20.2%patients.The multivariate analysis indicated there to be a statistically signiﬁcant relation of being higher at risk of developing depression,anxiety and stress when suffering from an active form of IBD(OR 6.487;95%Cl 1.220–34.500,P=0.028).Almost one third(30.9%)of patients have at least one of the

关键词： Inflammatory bowel disease depression anxiety stress COVID-19

Dynamic changes of behaviors, dentate gyrus neurogenesis and hippocampal miR-124 expression in rats with depression induced by chronic unpredictable mild stress

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Neural Regeneration Research 2020年第6期15卷 1150-1159页

作者： Yun-Ling Huang Ning-Xi Zeng Jie Chen Jie Niu Wu-Long Luo Ping Liu Can Yan Li-Li Wu Research Center for Basic Integrative Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Department of Pharmacology PLA General HospitalBeijingChina

The depression-like behavior phenotype,neurogenesis in the dentate gyrus and miR-124 expression in the hippocampus are the focus of current research on the pathogenesis of depression and antidepressant therapy.The present study aimed to clarify the dynamic changes of depression-like behavior,dentate gyrus neurogenesis and hippocampal miR-124 expression during depression induced by chronic stress to reveal pathological features at different stages of depression and to further provide insight into depression treatment.Chronic unpredictable mild stress depression models were established by exposing Sprague-Dawley rats to various mild stressors,including white noise,thermal swimming,stroboscopic illumination,soiled cages,pairing with three other stressed animals,cold swimming,tail pinch,restraint and water and food deprivation.Chronic unpredictable mild stress model rats underwent dynamic observation from 1 to 8 weeks and were compared with a control group(normal feeding without any stressors).To observe changes in the depression-like behavior phenotype during chronic unpredictable mild stress-induced depression,a sucrose preference test was used to evaluate the degree of anhedonia.An open-field test was used to evaluate locomotor activity and anxiety status.Compared with the control group,chronic unpredictable mild stress rats lost weight but did not have a depression-like behavioral phenotype at 1-4 weeks.Chronic unpredictable mild stress rats presented decreased sucrose preference and locomotor activity at 5-8 weeks.In addition,chronic unpredictable mild stress rats did not have significant anxiety-like behavior during 1-8 weeks of modeling.To observe neurogenesis dysfunctions and changes in neuronal number in the dentate gyrus during chronic unpredictable mild stress-induced depression,markers(DCX and DCX/BrdU)of neural proliferation and differentiation and the neuronal marker NeuN were assessed by immunofluorescence.Compared with the control group,neurogenesis and the neuronal number in the dentate gyrus did not

关键词： chronic unpredictable mild stress model continuous observation depression depression-like behavior dynamic changes hippocampus miR-124 neurogenesis dysfunction neuronal loss

SISTE1 promotes abscisic acid-dependent salt stress-responsive pathways via improving ion homeostasis and reactive oxygen species scavenging in tomato

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Journal of Integrative Plant Biology 2020年第12期62卷 1942-1966页

作者： Xiaoqing Meng Jing Cai Lei Deng Ge Li Jian Sun Yonghua Han Tingting Dong Yang Liu Tao Xu Siyuan Liu Zongyun Li Mingku Zhu Institute of Integrative Plant Biology School of Life SciencesJiangsu Normal UniversityXuzhou 221116China Jiangsu Key laboratory of Phylogenomics&Comparative Genomics School of Life SciencesJiangsu Normal UniversityXuzhou 221116China State Key Laboratory of Plant Genomics National Centre for Plant Gene Research(Beijing)Institute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China

High salinity is one of the major limiting fac-tors that reduces crop productivity and quality.Herein,we report that small SALT TOLERANCE ENHANCER1(STE1)protein without any known conserved domains is required for tomato salt tolerance.Overexpression(OE)of SlSTE1 enhanced the tolerance to multiple chloride salts(NaCl,KCl,and LiCl)and oxidative stress,along with elevated antioxidant enzyme activities,increased abscisic acid(ABA)and chlorophyll contents,and reduced ma-londialdehyde(MDA)and reactive oxygen species(ROS)accumulations compared to that of wild-type(WT)plants.Moreover,decreased K^+efflux and increased H^+efflux were detected in the OE plants,which induced a higher K^+/Na^+ratio.In contrast,SlSTE1-RNAi plants dis-played decreased tolerance to salt stress.RNA-seq data revealed 1330 differentially expressed genes in the OE plants versus WT plants under salt stress,and the tran-scription of numerous and diverse genes encoding transcription factors,stress-related proteins,secondary metabolisms,kinases,and hormone synthesis/signaling-related proteins(notably ABA and 1-aminocyclopropane-1-carboxylate)was greatly elevated.Furthermore,SlSTE1-OE plants showed increased sensitivity to ABA,and the results suggest that SlSTE1 promotes ABA-dependent salt stress-responsive pathways by interacting with SlPYLs and SlSnRK2s.Collectively,our findings reveal that the small SlSTE1 protein confers salt tolerance via ABA sig-naling and ROS scavenging and improves ion homeo-stasis in tomato.

关键词： stress elevated reactive

STOP1 regulatory system: Centered on multiple stress tolerance and cellular nutrientmanagement

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Molecular Plant 2021年第10期14卷 1615-1617页

作者： Hiroyuki Koyama Liujie Wu Raj Kishan Agrahari Yuriko Kobayashi Molecular PlantApplied Biological Sciences Gifu University501-1193 GifuJapan School of Environment and Life Science Nanning Normal UniversityNanning530001 GuangxiP.R.China.

Transcriptional regulation plays a crucial role in plant adaptation to diverse environments.Several transcription factors(TFs),the so-called master switch TFs or hub TFs,regulate various genes critical for adaptation to different stresses.STOP1(SENSITIVE TO PROTON RHIZOTOXICITY 1),a zinc-finger TF of Arabidopsis(Arabidopsis thaliana),is one such master/hub TF that transcriptionally regulates multiple stress tolerance(Sadhukhan et al.,2021).STOP1 plays a critical role in tolerance to acid soil syndrome(i.e.,H+and Al3+tolerance)(Iuchi et al.,2007)and hypoxia tolerance(Enomoto et al.,2019),and negatively regulates drought tolerance(Sadhukhan et al.,2019).In addition,Tian et al.

关键词： STOP1 stress Arabidopsis

Microglia-Derived NLRP3 Activation Mediates the Pressor Effect of Prorenin in the Rostral Ventrolateral Medulla of stress-Induced Hypertensive Rats

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Neuroscience Bulletin 2020年第5期36卷 475-492页

作者： Li Hu Shutian Zhang Kokwin Ooi Xuehai Wu Jiaxiang Wu Jian Cai Yinggang Sun Jijiang Wang Danian Zhu Fuxue Chen Chunmei Xia Laboratory of Neuropharmacology and Neurotoxicology Shanghai Key Laboratory of Bio-Energy CropsCollege of Life ScienceShanghai UniversityShanghai 200444China Department of Physiology and Pathophysiology Basic Medicine CollegeFudan UniversityShanghai 200032China Department of Neurosurgery Huashan HospitalFudan UniversityShanghai 200040China Department of Neurology Renji HospitalShanghai Jiaotong UniversityShanghai 200240China Department of Cardiovascular Diseases Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China

Increased microglial activation and neuroinflammation within autonomic brain regions such as the rostral ventrolateral medulla(RVLM)have been implicated in stress-induced hypertension(SIH).Prorenin,a member of the brain renin-angiotensin system(RAS),can directly activate microglia.The present study aimed to investigate the effects of prorenin on microglial activation in the RVLM of SIH rats.Rats were subjected to intermittent electric foot-shocks plus noise,this stress was administered for 2 h twice daily for 15 consecutive days,and mean arterial pressure(MAP)and renal sympathetic nerve activity(RSNA)were monitored.The results showed that MAP and RSNA were augmented,and this paralleled increased pro-inflammatory phenotype(M1)switching.Prorenin and its receptor(PRR)expression and the NLR family pyrin domain containing 3(NLRP3)activation were increased in RVLM of SIH rats.In addition,PLX5622(a microglial depletion agent),MCC950(a NLRP3 inhibitor),and/or PRO20(a(Pro)renin receptor antagonist)had antihypertensive effects in the rats.The NLRP3 expression in the RVLM was decreased in SIH rats treated with PLX5622.Mito-tracker staining showed translocation of NLRP3 from mitochondria to the cytoplasm in proreninstimulated microglia.Prorenin increased the ROS-triggering M1 phenotype-switching and NLRP3 activation,while MCC950 decreased the M1 polarization.In conclusion,upregulated prorenin in the RVLM may be involved in the pathogenesis of SIH,mediated by activation of the microglia-derived NLRP3 inflammasome.The link between prorenin and NLRP3 in microglia provides insights for the treatment of stress-related hypertension.

关键词： stress NLRP3 Prorenin Microglia Hypertension