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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 578-585页

作者： Wei-hong SUN Yong WANG Hua-gang HE Xue LI Wan SONG Bin DU Qing-wei MENG College of Food and Biological Engineering Jiangsu University State Key Laboratory of Crop Biology College of Life Science Shandong Agricultural University

Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic to- bacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress.

关键词： Methylviologen Oxidative stress StAPX stress tolerance Transgenic tobacco seedling

IiWRKY34 positively regulates yield, lignan biosynthesis and stress tolerance in Isatis indigotica

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Acta Pharmaceutica Sinica B 2020年第12期10卷 2417-2432页

作者： Ying Xiao Jingxian Feng Qing Li Yangyun Zhou Qitao Bu Junhui Zhou Hexin Tan Yingbo Yang Lei Zhang Wansheng Chen Research and Development Center of Chinese Medicine Resources and Biotechnology Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Changzheng HospitalNaval Medical University(Second Military Medical University)Shanghai 200003China Department of Pharmaceutical Botany School of PharmacyNaval Medical University(Second Military Medical University)Shanghai 200433China National Resource Center for Chinese Meteria Medica China Academy of Chinese Medical SciencesBeijing 100700China Kanion Research Institute Shanghai University of Traditional Chinese MedicineShanghai 201203China

Yield potential,pharmaceutical compounds production and stress tolerance capacity are 3 classes of traits that determine the quality of medicinal *** autotetraploid Isatis indigotica has greater yield,higher bioactive lignan accumulation and enhanced stress tolerance compared with its diploid *** we show that the transcription factor IiWRKY34,with higher expression levels in tetraploid than in diploid ***,has large pleiotropic effects on an array of traits,including biomass growth rates,lignan biosynthesis,as well as salt and drought stress *** analysis of transcriptome and metabolome profiling demonstrated that IiWRKY34 expression had far-reaching consequences on both primary and secondary metabolism,reprograming carbon flux towards phenylpropanoids,such as lignans and ***-metabolite correlation analysis was applied to construct the regulatory network of IiWRKY34 for lignan *** candidate target Ii4CL3,a key rate-limiting enzyme of lignan biosynthesis as indicated in our previous study,has been demonstrated to indeed be activated by ***,the results indicate that the differentially expressed IiWRKY34 has contributed significantly to the polyploidy vigor of ***,and manipulation of this gene will facilitate comprehensive improvements of *** herb.

关键词： Polyploidy vigor WRKY transcription factor Biomass production Lignan biosynthesis stress tolerance

Mapping of QTLs for Leaf Malondialdehyde Content Associated with stress tolerance in Rice

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Rice science 2009年第1期16卷 72-74页

作者： JIANG Jing ZHUANG Jie-yun FAN Ye-yang SHEN Bo College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310012 China Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China

Malondialdehyde （MDA） is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line （RIL） population with 247 lines derived from an indica-indica cross Zhenshan 97BxMilyang 46, and a linkage map consisting of 207 DNA markers were used. The RIL population showed a transgressive segregation in the MDA content of rice leaves. Two QTLs for the MDA content in rice leaves were detected in the intervals RG532-RG811 and RG381-RG236 on chromosome 1, with the additive effects from maternal and paternal parents, accounting for 4.33% and 4.62% of phenotype variations, respectively.

关键词： rice malondialdehyde content quantitative trait locus leaf stress tolerance

Relationship between Ethanol and 2-phenylethanol stress tolerance and Fatty Acid Compositions of Saccharomyces cerevisiae

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Journal of Food Science and Engineering 2013年第2期3卷 71-78页

作者： Olga Mameeva Valentin Podgorsky Department of Physiology of Industrial Microorganisms Zabolotny Institute of Microbiology and Virology of National Academy ofSciences of Ukraine Kiev D03680 Ukraine

In the present study, we investigated of the ethanol （Eth）, 2-phenylethanol （2-PE） and ethanol/2-phenylethanol （Eth/2-PE） stress tolerance and established relationship between stress tolerance and fatty acid compositions of wine yeast strain S. cerevisiae UCM Y-524 in relation to different cultivation factors. Changes in cell membrane fatty acid profiles studied under different temperature and media. The active oxygenation, semi-aerobic cultivation supplemented Tween 80 conditions were used in different combinations. The concentrations of 2-PE and Eth, the fatty acids methyl esters （FAMES） in the media were measured by GC-MS analyses. 2-PE, Eth, FAMES were identified by NIST02 MS database at the 2-PE and Eth standard solution （Merck, Germany）, bacterial FAMES standard （Supelco）. Palmitoleic and oleic acid were dominated for S. cerevisiae UCM Y-524. The unsaturated/saturated fatty acid （UFA/SFA） ratio was higher than five for S. cerevisiae UCM Y-524 at 14 ~C in simple medium and about more than three for other conditions. It has been found that yeast cells grown in the presence of the 2-PE, Eth and Eth/2-PE appear to increase the amount of unsaturated fatty acids, especially oleic acid, in cellular lipids especially under active oxygenation supplemented with Tween 80. S. cerevisiae UCM Y-524 has a higher tolerance under 2-PE, Eth and Eth/2-PE stress at 28 ~C in a simple medium under aerobic conditions. A higher concentration of unsaturated fatty acids in the membrane S. cerevisiae UCM Y-524 and a lower temperature under aerobic conditions, has been considered to have a positive influence on the excretion of 2-phenylethanol.

关键词： Yeasts 2-phenylethanol ethanol stress tolerance fatty acids.

Phenotypic, stress tolerance and Plant Growth Promoting Characteristics of Rhizobial Isolates from Selected Wild Legumes of Semiarid Region, Tirupati, India

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Advances in Microbiology 2016年第1期6卷 1-12页

作者： Y. Bhargava J. S. R. Murthy T. V. Rajesh Kumar M. Narayana Rao Department of Biotechnology S. V. University Tirupati India Department of Botany S. V. University Tirupati India

Rhizobia are vital for nitrogen input, fertility of soil and legume plant growth. Knowledge on rhizobial diversity from arid and semiarid areas is important for dry land agriculture in the context of climatic change and for economic utilization. This study provides morphological, biochemical, stress tolerance and plant growth promoting characteristics of fifteen rhizobial isolates from the nodules of same number of wild legumes and one isolate from cultivated Arachis hypogea from semi-arid region, Tirupati. The bacterial isolates were confirmed as rhizobia based on colony morphology and biochemical tests. Based on the colour change of YMA-BTB medium, eight isolates were identified as slow growers and six were fast growers. The isolates differed in growth pattern, colony morphology, antibiotic resistance at higher concentrations and uniformity in utilization of carbon and nitrogen sources. The isolates are tolerant to NaCl up to one percent, displayed normal growth at temperatures 28℃ - 30℃, at neutral pH and poor growth at pH 5and 9. The isolates varied in the production of EPS and IAA, positive for phosphate solubilization and siderophore formation. This functional diversity displayed by the isolates can be utilised for the legume crop production by cross inoculation.

关键词： Wild Legumes Rhizobia Characterization stress tolerance Plant Growth Promotion

Roles of MicroRNAs in Plant stress tolerance

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Journal of Northeast Agricultural University(English Edition) 2008年第3期15卷 68-74页

作者： GAO Peng YANG Liang BAI Xi ZHU Yanming College of Life Sciences Northeast Agricultural University Harbin 150030 China

MicroRNAs （miRNAs） are one kind of small RNA in all eukaryote. MicroRNAs can regulate gene expression of eukaryote; they widely participate in every physiological process. They can block mRNA expression or cleave mRNA by complement to target mRNA. Scholars estimate miRNA genes occuping about 1% of genome, but they can regulate 10%-30% genes of whole genome. The genes are regulated by miRNA including signal proteins, enzymes, transcription factors, and so on. In the field of plant research, the start of miRNA research is later, but it is proved that plant miRNAs are important to every plant physiological process. Now miRNA has become the hotspot of plant molecular biology research. This paper introduced the biology function, action mechanism, researching method and recently development of microRNAs, also focused on advances in plant microRNAs. This paper has important reference value for plant stress tolerance miRNA research.

关键词： microRNA gene expression plant stress tolerance

Research Progress of miR164 and Target Gene NAC in Regulating Sugarcane stress tolerance

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Plant Diseases and Pests 2023年第4期14卷 44-47页

作者： Minghui CHEN Junqing WANG Xuyao ZHAO Shiping CHENG Henan Provincial Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Wood Plant Pingdingshan UniversityPingdingshang 467000China Guangxi Key Laboratory of Sugarcane Genetic Improvement Nanning 530000China

Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to *** the main target gene of miR164,NAC transcription factors are mainly regulated at the post-transcriptional ***164:NAC module may play an important role in determining the adaptive response of sugarcane to ***164 has a regulatory effect on the expression of target gene NAC,and may be closely related to the resistance process of sugarcane to abiotic stress,which provides a reference for using miRNA to carry out sugarcane resistance molecular breeding.

关键词： miRNA164 NAC stress tolerance

The hli Gene Family:Function and Potential Application in Plant stress tolerance

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The hli Gene Family:Function and Potential Application in Pl...

Fibroblast growth factor 21 prolongs lifespan and improves stress tolerance in the silkworm,Bombyx mori

第二届植物分子育种国际会议

作者： S.Jantaro Department of Applied Science University of Arkansas at Little RockLittle RockAR72204USA

＜正＞The hli genes, encoding the high light inducible proteins （HLIP） with similarity to the light harvesting chlorophyll a/b-binging proteins of vascular plants, are induced under a variety of stress conditions. T... 详细信息

关键词： hli gene Photosynthesis stress tolerance Cyanobacteria Iron homeostasis

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Fibroblast growth factor 21 prolongs lifespan and improves s...

Wheat Zinc Finger Protein Gene TaCHP is Negatively Regulated by ABA and Salinity stress and Plays a Positive Role in stress tolerance

中国蚕学会2021年学术年会

作者： Jiang-Bo Song Kai-Ge Hao Xin Chen Yan-Hua Zhang Zi-Lin Cheng Shuang Mao Yong-Xi Tang Xiao-Ling Tong Fang-Yin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest University

Background:Fibroblast growth factor 21（FGF21）,an FGF family member,is an atypical hormone and pro-longevity ***:To better understand of the effects of exogenous administration of FGF21 on lifespan and stress tolerance,and the underlying molecular basis,we used the silkworm,Bombyx mori,as an experimental animal model to evaluate FGF21’s pharmaceutical ***:Lifespan was significantly prolonged in female silkworms with FGF21 replenishment,whereas no effect was observed in the male ***21 replenishment also significantly improved the activity of antioxidant systems such as glutathione-S-transferase（GST） and superoxide dismutase（SOD） and significantly decreased malondialdehyde（MDA） ***,FGF21 was found to play a critical role in enhancing stress resistance,including ultraviolet（UV） irradiation tolerance and ***,AMPK,Fox O,and sirtuins were activated by FGF21 and may be responsible for the prolonged lifespan and enhanced antioxidant activity observed in ***:Collectively,the results suggest the molecular pathways underlying of FGF21-induced longevity and stress tolerance,and support the use of silkworms as a promising experimental animal model for evaluating the pharmaceutical effects of small molecules.

关键词： Bombyx mori fibroblast growth factor 21 (FGF21) lifespan oxidation resistance stress tolerance

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Wheat Zinc Finger Protein Gene TaCHP is Negatively Regulated...