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SCAB1 coordinates sequential Ca^(2+) and ABA signals during osmotic stress induced stomatal closure in Arabidopsis

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Science China(Life Sciences) 2024年第1期67卷 1-18页

作者： Tianren Zhang Li Bai Yan Guo State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Hyperosmotic stress caused by drought is a detrimental threat to plant growth and agricultural productivity due to limited water *** are gateways of transpiration and gas exchange,the swift adjustment of stomatal aperture has a strong influence on plant drought *** intensive investigations of stomatal closure during drought stress in past decades,little is known about how sequential signals are integrated during complete ***,we discovered that the rapid Ca^(2+) signaling and subsequent abscisic acid(ABA)signaling contribute to the kinetics of both F-actin reorganizations and stomatal closure in Arabidopsis thaliana,while stomatal CLOSURE-RELATED ACTIN BINDING PROTEIN1(SCAB1)is the molecular switch for this entire *** the early stage of osmotic shock responses,swift elevated calcium signaling promotes SCAB1 phosphorylation through calcium sensors CALCIUM DEPENDENT PROTEIN KINASE3(CPK3)and *** phosphorylation restrained the microfilament binding affinity of SCAB1,which bring about the Factin disassembly and stomatal closure *** the osmotic stress signal continued,both the kinase activity of CPK3 and the phosphorylation level of SCAB1 attenuated *** further found that ABA signaling is indispensable for these attenuations,which presumably contributed to the actin filament reassembly process as well as completion of stomatal ***,the dynamic changes of SCAB1 phosphorylation status are crucial for the kinetics of stomatal *** together,our results support a model in which SCAB1 works as a molecular switch,and directs the microfilament rearrangement through integrating the sequentially generated Ca^(2+) and ABA signals during osmotic stress induced stomatal closure.

关键词： calcium signal ABA stomatal movement microfilaments kinase

Ca^(2+)-independent ZmCPK2 is inhibited by Ca^(2+)-dependent ZmCPK17 during drought response in maize

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Journal of Integrative Plant Biology 2024年第7期66卷 1313-1333页

作者： Xiaoying Hu Jinkui Cheng Minmin Lu Tingting Fang Yujuan Zhu Zhen Li Xiqing Wang Yu Wang Yan Guo Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design BreedingCenter for Crop Functional Genomics and Molecular BreedingCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 071002China

Calcium oscillations are induced by different ***-dependent protein kinases(CDPKs/CPKs)are one major group of the plant calcium decoders that are involved in various processes including drought *** CPKs are ***,we identified ZmCPK2 as a negative regulator of drought resistance by screening an overexpression transgenic maize *** found that ZmCPK2 does not bind calcium,and its activity is mainly inhibited during short term abscisic acid(ABA)treatment,and dynamically changed in prolonged ***,ZmCPK2 interacts with and is inhibited by calcium-dependent ZmCPK17,a positive regulator of drought resistance,which is activated by ***17 could prevent the nuclear localization of ZmCPK2 through phosphorylation of ***2 interacts with and phosphorylates and activates ZmYAB15,a negative transcriptional factor for drought *** results suggest that drought stress-induced Ca^(2+)can be decoded directly by ZmCPK17 that inhibits ZmCPK2,thereby promoting plant adaptation to water deficit.

关键词： CDPK drought response phosphorylation stomatal movement transcriptional regulation

Extracellular ATP Promotes stomatal Opening of Arabidopsis thaliana through Heterotrimeric G Protein α Subunit and Reactive Oxygen Species

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Molecular Plant 2012年第4期5卷 852-864页

作者： Li-Hua Hao Wei-Xia Wang Chen Chen Yu-Fang wang Ting Liu Xia Li Zhong-Lin Shang a Key Laboratory of Molecular and Cell Biology of Hebei Province College of Life SciencesHebei Normal UniversityShijiazhuang 050016RR.China State Key Laboratory of Plant Cell and Chromosome Engineering Center of Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021P.R.China

In recent years, adenosine tri-phosphate （ATP） has been reported to exist in apoplasts of plant cells as a signal molecule. Extracellular ATP （eATP） plays important roles in plant growth, development, and stress tolerance. Here, extra- cellular ATP was found to promote stomatal opening of Arabidopsis thaliana in light and darkness. ADP, GTP, and weakly hydrolyzable ATP analogs （ATPγS, Bz-ATP, and 2meATP） showed similar effects, whereas AMP and adenosine did not affect stomatal movement. Apyrase inhibited stomatal opening. ATP-promoted stomatal opening was blocked by an NADPH oxidase inhibitor （diphenylene iodonium） or deoxidizer （dithiothreitol）, and was impaired in null mutant of NADPH ox- idase （atrbohD/F）. Added ATP triggered ROS generation in guard cells via NADPH oxidase. ATP also induced Ca^2＋ influx and H ＋ efflux in guard cells. In atrbohD/F, ATP-induced ion flux was strongly suppressed. In null mutants of the heterotrimeric G protein α subunit, ATP-promoted stomatal opening, cytoplasmic ROS generation, Ca^2＋ influx, and ^H＋ efflux were all sup- pressed. These results indicated that eATP-promoted stomatal opening possibly involves the heterotrimeric G protein, ROS, cytosolic Ca^2＋, and plasma membrane H＋-ATPase.

关键词： Extracellular ATP stomatal movement Arabidopsis thaliana heterotrimeric G protein reactive oxygenspecies.

Protein Tyrosine Phosphatases Mediate the Signaling Pathway of stomatal Closure of Vicia faba L.

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Journal of Integrative Plant Biology 2005年第3期47卷 319-326页

作者： Wu-LiangSHI XinLIU Wen-SuoJIA Shu-QiuZHANG StateKeyLaboratoryofPlantPhysiologyandBiochemistry CollegeofBiologicalSciencesChinaAgriculturalUniversityBeijing100094China DeportmentofLifeSciences LaiyangAgriculturalCollegeLaiyang265200China CollegeofAgronomyandBiotechnology ChinaAgriculturalUniversityBeijing100094China

The regulation of stomatal movement is one of the most important signaling networks in plants. The H+-ATPase at the plasma membrane of guard cells plays a critical role in the stomata opening, while there are some conflicting results regarding the effectiveness of the plasma membrane H+-ATPase inhibitor, vanadate, in inhibiting stomata opening. We observed that 2 mmol/L vanadate hardly inhibited light-stimulated stomata opening in epidermal peels of Vicia faba L., but significantly inhibited dark- and ABA-induced stomatal closure. These results cannot be explained with the previous findings that H+-ATPase was inhibited by vanadate. In view of the fact that vanadate is an inhibitor of protein tyrosine phosphatases (PTPases), we investigated whether the stomatal movement regulated by vanadate is through the regulation of PTPase. As expected, phenylarsine oxide (PAO), a specific inhibitor of PTPase, has very similar effects and even more effective than vanadate. Typical PTPase activity was found in guard cells of V. faba; moreover, the phosphatase activity could be inhibited by both vanadate and PAO. These results not only provide a novel explanation for conflicting results about vanadate modulating stomatal movement, but also provide further evidence for the involvement of PTPases in modulating signal transduction of stomatal movement.

关键词： abscisic acid (ABA) H+-ATPase protein tyrosine phosphatases stomatal movement Vicia faba L

Carbonic anhydrases are upstream regulators of CO2 signaling in guard cells

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Carbonic anhydrases are upstream regulators of CO2 signaling...

从植物科学到农业发展——2012全国植物生物学大会

作者： Julian I.Schroeder Cell and Developmental Biology Section Division of Biological SciencesUniversity of CaliforniaSan DiegoLa JollaCA 92093-0116USA

We know ambient CO is continuing rising and this rising CO causes plants stomatal pores closing and thus regulates plant gas exchange---CO influx into plants and water loss by ***,little is known about the early CO sensing mechanisms that control plant gas ***,we found that CO-binding proteins,βCA1 andβCA4, are required for[CO]regulation of stomatal movements and also stomatal development, functioning as the earliest components in guard cell signaling ***, CA-mediated stomatal CO responses are not directly linked to photosynthesis and originate in guard *** cell over-expression of either CA1 or CA4 in wild-type plants greatly enhances water use *** physiological research showed that CA-mediated catalysis is a major mechanism forβCA-mediated CCvinduced stomatal closing and elevated HCO,more than CO and protons,functions as a small-molecule transducer to activate anion channels in guard *** this pathway,Cais required for HCO activation of S-type anion channel and the activation is mediated by SLAC1. Furthermore,several proteins were identified as interactors of CA1 and CA4,indicating there is another mechanism for CA1 and CA4 in CO regulation of stomatal movement. The detailed data will be presented at the meeting.

关键词： βCA1 andβCA4 CO2 HCO3- guard cell stomatal movement signaling

Arabidopsis IQM1 is involved in stomatal innate immune

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Arabidopsis IQM1 is involved in stomatal innate immune

广东省遗传学会第九届代表大会暨学术研讨会

作者：周玉萍 Qiong-Hua Chen Xiao-Ling Huang Zhang-Ke Huang Zhong-Zhen Mo 田长恩 School of life Sciences Guangzhou University

Arabidopsis IQMs share sequence homology with a pea heavy metal-induced protein 6 and a *** expression patterns for each gene suggest that each IQM family member may play a different role in plant development and response to environmental *** functions of the IQM family members remain to be *** can bind with AtCaM5 in yeast two-hybrid assay via its *** CaM binding was Ca-independent in vitro,and was also observed in bimolecular fluorescence complementation analyses in onion epidermal *** was found to express strongly in guard cells and the cortex of *** T-DNA insertion mutants of IQMl(iqml) displayed a smaller stomatal aperture,a decreased water loss rate and a shorter primary ***,iqml did not change its stomatal aperture when treated with light,dark,ABA and chitin *** guard cell of iqml-1 also showed enhanced expression of genes involved in production and signaling of reactive oxygen species(ROS).Consistently,increased ROS level was observed in guard ***,243 and 28 genes were up- and down-regulated by more than two fold in the leaf of iqml-1,***,34 of 117 and 7 of 30 chitin-responsive transcriptional factor and ubiquitin ligase genes were *** 51defense-associated genes in iqml-1 were also *** with above,iqml-1 was found to be more resistant to Pst DC3000 than wild type when the pathogen is sprayed,but to show similar symptom to wild type when the pathogen is infiltrated,suggesting that IQMl is involved in stomatal innate *** further results showed that mutation of IQMl reduces sensitivity to J A which enhances stomatal aperture and consequently reduces resistance to Pst DC3000,indicating that IQMl enhance J A signal transduction and stomatal open and consequently reduce resistance to Pst DC3000.

关键词： AtIQM1 stomatal movement Reactive oxygen species Calmodulin-binding protein JA signaling Arabidopsis thaliana stomatal innate immune Pst DC3000

Role and interrelationship of Gαprotein,hydrogen peroxide and nitric oxide in UV-B-induced stomatal closure in Arabidopsis leaves

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Role and interrelationship of Gαprotein,hydrogen peroxide a...

从植物科学到农业发展——2012全国植物生物学大会

作者： Xiange Ma,Ying Zhang,Junmin He~* School of Life Sciences,Shaanxi Normal University,Xi’an 710062,China

We found that 0.5 W m" UV-B triggered a significant increase in hydrogen peroxide(HO) or nitric oxide(NO) levels associated with stomatal closure in wild type,but these effects were abolished in the single and double mutants of AtrbohD and AtrbohF or in the Nial mutants ***,we found that UV-B-mediated HO and NO generation are regulated by GPA1,the Ga-subunit of heterotrimeric G ***-B-dependent HO and NO accumulation were nullified in gpal knockout mutants but enhanced by overexpression of a constitutively active form of GPA1(cGα).In addition,exogenously applied HO or NO rescued the defect in UV-B-mediated stomatal closure in gpal mutants, whereas cGa AtrbohD/F and cGa nia1 constructs exhibited a similar response as AtrbohD/F and Nial did ***,we demonstrated that Ga activation of NO production depends on *** mutants of AtrbohD and AtrbohF had impaired NO generation in response to UV-B,but UV-B-induced H2O accumulation was not impaired in ***,exogenously applied NO rescued the defect in UV-B-mediated stomatal closure in the mutants of AtrbohD and *** findings establish a signaling pathway leading to UV-B-induced stomatal closure,which involves GPA1-dependent activation of HO production and subsequent Nia1-dependent NO accumulation.

关键词： Gαprotein hydrogen peroxide nitric oxide UV-B stomatal movement Arabidopsis thaliana

Hydrogen Peroxide Signaling on Photosynthetic Characteristics of Transgenice Rice Overexpression Maize C4 Pepc

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Hydrogen Peroxide Signaling on Photosynthetic Characteristic...

第二届国际整合植物生物学学术研讨会

作者： Xia Li~* and Chenggang Ren Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement,Nanjing 210014,China

Phosphoenolpymvate carboxylase(pepc) of maize,the crucial enzyme in the C photosynthetic pathway,has been successfully transformed into C crop rice and is highly *** transgenic rice’s photosynthetic rate has also been ***,the improvement of C crops’ photosynthetic efficiency by transforming C-photosynthetic specialty has attracted more and more *** this discovery,an increasing amount of research on rice breeding showed that pepc transgenic rice also has tolerance to oxidative stress,especially from high intensity light,and it was clearly related to the *** the important functional molecule of oxidative stress,there are many ways in which hydrogen peroxide may be generated,as it plays an important role inside plant *** paper mainly investigates the relationship between the photosynthetic specialty of C-pepc transgenic rice(PC) and hydrogen peroxide *** results are as follows:*** characteristics of PC and untransformed rice plant(WT) were investigated under different concentrations of exogenous *** compared with WT,PN of PC treated with low level HO(0.5～5 mmol/L HO) decrease with changing G,and the inhibition is weakened as light intensity *** low concentrations of HO might regulate Gs of PC to affect physiological characteristics of photosynthesis;*** method of regents inhale from petiole cut,regents related to endogenous HO such as abscisic acid(ABA), phospholipase D inhibition n-butanol(BA),phospholipase C inhibition neomycin(NS) and diphenyleneiodonium(DPI) were *** ABA treatment,both PC and WT’s endogenous HO concentrations were *** compared to WT,PC with BA and DPI treatments can all inhibit PC’s the endogenous generation of HO This was similar to results on the measurement of endogenous ***,the effects of endogenous HO on PC’s PN were *** treatment with DPI for 30 minutes,PC and WT behave *** of WT does not change much,bu

关键词： Oryza sativa L. C4 PEPC H2O2 photosynthesis stomatal movement