检索结果-南通市图书馆

Arabidopsis Squalene Epoxidase 3 （SQE3） Complements SQE1 and Is Important for Embryo Development and Bulk Squalene Epoxidase Activity

引用

Molecular Plant 2015年第7期8卷 1090-1102页

作者： Sara Laranjeira Vitor Amorim-Silva Alicia Esteban Monserrat Arro Albert Ferrer Rui Manuel Tavares Miguel Angel Botella Abel Rosado Herlander Azevedo Center for Biodiversity Functional & Integrative Genomics (BioFIG) Plant Functional Biology Center University of Minho Campus de Gualtar 4710-057 BragaPortugal Instituto de Hortofruticultura Subtropical y Mediterranea 'La Mayora' Universidad de Malaga-Consejo Superior de Investigaciones Cientificas (IHSM-UMA-CSIC)Departmento de Biologia Molecular y Bioquimica Universidad de Malaga Campus Teatinos 29071 Malaga Spain Department of Molecular Genetics Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Bellaterra-Cerdanyola del Valles 08193 Spain Department of Biochemistry and Molecular Biology Faculty of Pharmacy University of Barcelona 08028 Barcelona Spain Canada Research Chair in Plant Physiology and Cellular Dynamics Department of Botany Faculty of Sciences University of British Columbia VancouverBC V6T 1Z4 Canada CIBIO InBIO - Research Network in Biodiversity and Evolutionary Biology Universidade do Porto Campus Agrario de Vairao 4485-661 Vairao Portugal

The existence of multigenic families in the mevalonate pathway suggests divergent functional roles for pathway components involved in the biosynthesis of plant sterols. Squalene epoxidases （SQEs） are key components of this pathway, and Squalene Epoxidase 1 （SQE1） has been identified as a fundamental enzyme in this biosynthetic step. In the present work, we extended the characterization of the remaining SQE family members, phylogenetically resolving between true SQEs and a subfamily of SQE-like proteins that is exclusive to Brassicaceae. Functional characterization of true SQE family members, Squalene Epox- idase 2 （SQE2） and Squalene Epoxidase 3 （SQE3）, indicates that SQE3, but not SQE2, contributes to the bulk SQE activity in Arabidopsis, with sqe3-1 mutants accumulating squalene and displaying sensitivity to ter- binafine. We genetically demonstrated that SQE3 seems to play a particularly significant role in embryo development. Also, SQE1 and SQE3 both localize in the endoplasmic reticulum, and SQE3 can functionally complement SQEI. Thus, SQE1 and SQE3 seem to be two functionally unequal redundant genes in the pro- motion of plant SQE activity in Arabidopsis.

关键词： Arabidopsis embryo development MVA pathway squalene epoxidase sterol biosynthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：