检索结果-南通市图书馆

Neuroprotective effects of BDNF and GDNF in intravitreally transplanted mesenchymal stem cells after optic nerve crush in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2017年第1期10卷 35-42页

作者： Zong-Li Hu Ni Li Xin Wei Li Tang Ting-Hua Wang Xiao-Ming Chen Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Institute of Neurological Disease West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

AIM： To assess the neuro-protective effect of bone marrow mesenchymal stem cells （BMSCs） on retinal ganglion cells （RGCs） following optic nerve crush in mice. METHODS： C56BL/6J mice were treated with intravitreal injection of PBS, BMSCs, BDNF-interference BMSCs （BIM）, and GDNF-interference BMSCs （GIM） following optic nerve crush, respectively. The number of surviving RGCs was determined by whole-mount retinas and frozen sections, while certain mRNA or protein was detected by q-PCR or ELISA, ***： The density （cell number/mm^2） of RGCs was 410.77±56.70 in the retina 21d after optic nerve crush without any treatment, compared to 1351.39±195.97 in the normal control （P〈0.05）. RGCs in BMSCs treated eyes was 625.07±89.64/mm^2, significantly higher than that of no or PBS treatment （P〈0.05）. While RGCs was even less in the retina with intravitreal injection of BIM （354.07±39.77） and GIM （326.67±33.37） than that without treatment （P〈0.05）. BMSCs injection improved the internal BDNF expression in ***： Optic nerve crush caused rust loss of RGCs and intravitreally transplanted BMSCs at some extent protected RGCs from death. The effect of BMSCs and level of BDNF in retinas are both related to BDNF and GDNF expression in BMSCs.

关键词： optic nerve crash retinal ganglion cells stemcells brain-derived neurotrophie factor glial cell line-derived neurotrophic factor

Human urokinase-type plasminogen activator gene-modifiedbone marrow-derived mesenchymal stem cells attenuateliver fibrosis in rats by down-regulating the Wnt signalingpathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第6期22卷 2092-2103页

作者： Zhi-Gang Ma Xiao-Dan Lv Ling-Ling Zhan Lan Chen Qi-Yuan Zou Ji-Qiao Xiang Jiao-Li Qin Wei-Wei Zhang Zhao-Jing Zeng Hui Jin Hai-Xing Jiang Xiao-Ping Lv Department of Gastroenterology the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Clinical Experimental Medicine the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

AIM: To evaluate the therapeutic effects of bone marrow-derived mesenchymal stem cells(BMSCs) with human urokinase-type plasminogen activator(u PA) on liver fibrosis, and to investigate the mechanism of gene ***: BMSCs transfected with adenovirusmediated human urokinase plasminogen activator(Adu PA) were transplanted into rats with CCl4-induced liver fibrosis. All rats were sacrificed after 8 wk, and their serum and liver tissue were collected for biochemical, histopathologic, and molecular analyzes. The degree of liver fibrosis was assessed by hematoxylin and eosin or Masson's staining. Western blot and quantitative reverse transcription-polymerase chain reaction were used to determine protein and m RNA expression ***: Serum levels of alanine aminotransferase, aminotransferase, total bilirubin, hyaluronic acid, laminin, and procollagen type Ⅲ were markedly decreased, whereas the levels of serum albumin were increased by u PA gene modified BMSCs treatment. Histopathology revealed that chronic CCl4-treatment resulted in significant fibrosis while u PA gene modified BMSCs treatment significantly reversed fibrosis. By quantitatively analysing the fibrosis area of liver tissue using Masson staining in different groups of animals, we found that model animals with CCl4-induced liver fibrosis had the largest fibrotic area(16.69% ± 1.30%), while fibrotic area was significantly decreased by BMSCs treatment(12.38% ± 2.27%) and was further reduced by u PA-BMSCs treatment(8.31% ± 1.21%). Both protein and m RNA expression of β-catenin, Wnt4 and Wnt5 a was down-regulated in liver tissues following u PA gene modified BMSCs treatment when compared with the model ***: Transplantation of u PA gene modified BMSCs suppressed liver fibrosis and ameliorated liver function and may be a new approach to treating liver fibrosis. Furthermore, treatment with u PA gene modified BMSCs also resulted in a decrease in expression of molecules of the Wnt signalin

关键词： bone marrow-derived mesenchymal stemcells liver fibrosis urokinase plasminogen activator Wnt signaling pathway

Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第21期21卷 6582-6590页

作者： Ya-Bin Yu Jian-Min Bian Dian-Hua Gu Department of Hepatobiliary Surgery Huai’an First People’s HospitalNanjing Medical University Department of General Surgery Nanjing First Hospital Affiliated to Nanjing Medical University

AIM:To investigate the effects of transplantation of insulin-producing cells(IPCs) in the treatment of diabetic rats after 90% ***:Human umbilical cord mesenchymal stem cells(UCMSCs) were isolated and induced into IPCs using differentiation *** cells were examined by dithizone(DTZ) staining,reverse transcription-polymerase chain reaction(RT-PCR),and real-time RT-PCR.C-peptide release,both spontaneously and after glucose challenge,was measured by *** were then transplanted into Sprague-Dawley rats after 90% pancreatectomy and blood glucose levels and body weight were ***:The differentiated cells were positive for DTZ staining and expressed pancreatic β-cell related genes.C-peptide release by the differentiated cells increased after glucose challenge(380.6 ± 15.32 pmol/L vs 272.4 ± 15.32 pmol/L,P < 0.05).Further,in the cell transplantation group,blood sugar levels were significantly lower than in the sham group 2 wk after transplantation(18.7 ± 2.5 mmol/L vs 25.8 ± 1.25 mmol/L,P < 0.05).Glucose tolerance tests showed that 45 min after intraperitoneal glucose injection,blood glucose levels were significantly lower on day 56 after transplantation of IPCs(12.5 ± 4.7 mmol/L vs 42.2 ± 9.3 mmol/L,P < 0.05).CONCLUSION:Our results show that UCMSCs can differentiate into islet-like cells in vitro under certain conditions,which can function as IPCs both in vivo and in vitro.

关键词： Human umbilical cord mesenchymal stemcells 90% pancreatectomy Diabetic mellitus

Current focus of stem cell application in retinal repair

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2015年第3期7卷 641-648页

作者： Maria L Alonso-Alonso Girish Kumar Srivastava Instituto Universitario de Oftalmobiologia Aplicada (IOBA) Universidad de Valladolid 47011 Valladolid Spain Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leon 47011 Valladolid Spain

The relevance of retinal diseases, both in society's economy and in the quality of people's life who suffer with them, has made stem cell therapy an interesting topic forresearch. Embryonic stem cells(ESCs), induced pluripotent stem cells(i PSCs) and adipose derived mesenchymal stem cells(ADMSCs) are the focus in current endeavors as a source of different retinal cells, such as photoreceptors and retinal pigment epithelial cells. The aim is to apply them for cell replacement as an option for treating retinal diseases which so far are untreatable in their advanced stage. ESCs, despite the great potential for differentiation, have the dangerous risk of teratoma formation as well as ethical issues, which must be resolved before starting a clinical trial. i PSCs, like ESCs, are able to differentiate in to several types of retinal cells. However, the process to get them for personalized cell therapy has a high cost in terms of time and money. Researchers are working to resolve this since i PSCs seem to be a realistic option for treating retinal diseases. ADMSCs have the advantage that the procedures to obtain them are easier. Despite advancements in stem cell application, there are still several challenges that need to be overcome before transferring the research results to clinical application. This paper reviews recent research achievements of the applications of these three types of stem cells as well as clinical trials currently based on them.

关键词： Retina Adipose derived mesenchymal stemcells Embryonic stem cells Induced pluripotent stemcells Cell therapy

Neural differentiation from embryonic stem cells in vitro : An overview of the signaling pathways

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2015年第2期7卷 437-447页

作者： Jen-Hua Chuang Li-Chu Tung Yenshou Lin Department of Life Science National Taiwan Normal University

Neurons derived from embryonic stem cells(ESCs) have gained great merit in both basic research and regenerative medicine. Here we review and summarize the signaling pathways that have been reported to be involved in the neuronal differentiation of ESCs,particularly those associated with in vitro differentiation. The inducers and pathways explored include retinoic acid, Wnt/b-catenin, transforming growth factor/bone morphogenetic protein, Notch, fibroblast growth factor, cytokine, Hedgehog, c-Jun N-terminal kinase/mitogen-activated protein kinase and others. Some other miscellaneous molecular factors that have been reported in the literature are also summarized and discussed. These include calcium, calcium receptor, calcineurin, estrogen receptor, Hox protein, ceramide, glycosaminioglycan, ginsenoside Rg1, opioids, two pore channel 2, nitric oxide, chemically defined medium, cellcell interactions, and physical stimuli. The interaction or crosstalk between these signaling pathways and factors will be explored. Elucidating these signals in detail should make a significant contribution to future progress in stem cell biology and allow, for example, better comparisons to be made between differentiation in vivo and in vitro. Of equal importance, a comprehensive understanding of the pathways that are involved in the development of neurons from ESCs in vitro will also accelerate their application as part of translational medicine.

关键词： Neurons Differentiation Embryonic stemcells Signaling Pathways

Placental-derived stem cells:Culture, differentiation and challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2015年第4期7卷 769-775页

作者： Maira S Oliveira Joao B Barreto-Filho College of Veterinary Medicine Veterinary SchoolUniversidade Federal de Minas Gerais Department of Veterinary Medicine Universidade Federal de Lavras

Stem cell therapy is a promising approach to clinical healing in several diseases. A great variety of tissues(bone marrow, adipose tissue, and placenta) arepotentially sources of stem cells. Placenta-derived stem cells(p-SCs) are in between embryonic and mesenchymal stem cells, sharing characteristics with both, such as non-carcinogenic status and property to differentiate in all embryonic germ layers. Moreover, their use is not ethically restricted as fetal membranes are considered medical waste after birth. In this context, the present review will be focused on the biological properties, culture and potential cell therapy uses of placental-derived stem cells. Immunophenotype characterization, mainly for surface marker expression, and basic principles of p-SC isolation and culture(mechanical separation or enzymatic digestion of the tissues, the most used culture media, cell plating conditions) will be presented. In addition, some preclinical studies that were performed in different medical areas will be cited, focusing on neurological, liver, pancreatic, heart, muscle, pulmonary, and bone diseases and also in tissue engineering field. Finally, some challenges for stem cell therapy applications will be highlighted. The understanding of the mechanisms involved in the p-SCs differentiation and the achievement of pure cell populations(after differentiation) are key points that must be clarified before bringing the preclinical studies, performed at the bench, to the medical practice.

关键词： Fetal membrane Placenta Embryonic stemcells Mesenchymal stem cells Cell therapy