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Crystallization and preliminary X-ray analysis of SNR 141--An N-terminal fragment of staphylococcal nuclease R

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Chinese Science Bulletin 1997年第11期42卷 957-959页

作者： Ye, S Wan, ZL Zhou, B Jing, GZ Liang, DC CHINESE ACAD SCI INST BIOPHYSSTATE KEY LAB BIOMACROMOLBEIJING 100101PEOPLES R CHINA

staphylococcal nuclease(SNase A,EC 3.***.4.7),which hydrolyzes the phosphodiesterbond of DNA and RNA,and releases 3’-phosphate mononucleotides and dinucleotides,con-sists of 149 amino acid residues(MW=16 807)without sulfhydryl and disulfide *** A was originally derived from Staphylococcus *** the gene of the enzyme wascloned and inserted into several expression *** crystal structure was detected

关键词： crystallization staphylococcal nuclease R N-terminal fragment.

The Akt Pathway Inhibitor Degeulin Prevents staphylococcal Enterotoxin B Induced Splenocyte Proliferation and Inflammation

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Advances in Bioscience and Biotechnology 2017年第1期8卷 1-12页

作者： Sarah Joanne Christine Whitfield Jane Elizabeth Risdall Gareth Griffiths Ethel Diane Williamson Alun James Carter Department of Biomedical Sciences Defence Science and Technology Laboratory Porton Down Wiltshire UK Department of Anaesthesia University of Cambridge Cambridge UK Department of Medicine Washington University St. Louis Missouri USA

staphylococcal Enterotoxin B (SEB) is considered a potential biological weapon. It is toxic by both inhalation and ingestion. Effects of ingestion include fever, vomiting and diarrhoea, while inhalation may additionally result in chest pain, dyspnoea, pulmonary oedema and respiratory failure. Severe exposure may be fatal and treatment relies on symptomatic support. At a cellular level, SEB up-regulates T-cell proliferation leading to a pathological inflammatory response. Deguelin, a rotenoid isolated from the African plant Mundulea sericea (Leguminosae), has been shown to reduce cellular proliferation by inhibiting the phosphoinositide 3-kinase/Akt (PI3K/Akt) signalling pathway. Using isolated murine splenocytes, we have demonstrated that treatment with deguelin reduces SEB inducing T cell proliferation by 60%. Deguelin treatment also decreased IL-2 and CCL2 secretion by splenocytes exposed to SEB. We demonstrate that targeting cellular proliferation can significantly reduce inflammation after SEB exposure and suggest that anti-proliferatives may have a role as potential generic medical counter measures if superantigens are used as biological weapons.

关键词： staphylococcal Enterotoxin B Deguelin Therapy Inflammation Biological Weapon

Structural and biochemical characterization of the staphylococcal phage endolysin LysGH15

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Science Foundation in China 2015年第2期23卷 10-10页

With the support by the National Natural Science Foundation of China,*** Wenyu’s laboratory at the College of Veterinary Medicine,Jilin University,reported the structural and biochemical characterization of staphylococcal phage endolysin LysGH15,which was published in Plos Pathogens(2014,10(5):e1004109).LysGH15demonstrates a wide lytic spectrum and strong lytic activity against methicillin-resistant

关键词： staphylococcal phage biochemical methicillin Jilin groove lysin inactive residues guided

THE EFFECT OF SUPERANTIGEN staphylococcalENTEROTOXIN B AND D-GALACTOSAMINE ON BALB/CMOUSE HEPATOCYTES

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Medical Bulletin of Shanghai Jiaotong University 1999年第1期20卷 29-32,59页

作者：印彤童善庆朱佑明陆德源谢玉才

Objective To observe the role of superantigen staphylococcal enterotoxin B(SEB) andD - galactosamine (D - GalN) on Balb/c mouse hepatocytes and its mechanism. Methods After Balb/c mice wereinjected intraperitoneally with SEB, D- GalN or both, blood samples were collected and livers were removed at 2,6, 12, 24h. Patterns of hepatocellular death were studied morphologically and biochemically, circulating cytokines(TNF, IFN-γ) were determined, and mice mortality within 24h was assessed. Results SEB could induce thetypical apoptotic changes of hepatocytes morphologically and biochemically. The mechanism is probably associatedwith the production and release of Cytokines (such as TNF, IFN- γ, etc).D - GalN could induce hepatocytesapoptosis and degeneration at the same time. Besides this, we confirmed hepatocytes of the mice which wereadministered SEB and D - GalN developing apoptosis at 2, 6h, but after 12h hepatocytes were characterized bysevere injury, the mice mortality within 24h is 50%. Conclusion SEB or D - GalN alone could induce the typicalapoptotic changes of hepatocytes. SEB+D-GalN developed hepatocytes apoptosis in the early stage and necrosisin the later. It suggests that there is some relationship between hepatic cell apoptosis and necrosis, and massivehepatocyte apoptosis is the probably initiating step of acute hepatic necrosis in mice.

关键词： superantigen staphylococcal enterotoxin B D - galactosamine apoptosisacute hepatic necrosis

Monoclonal antibody, a novel probe for protein folding

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Science China(Life Sciences) 1998年第2期41卷 163-168页

作者：程鹏王锡德静国忠赵康源周筠梅郭振泉 1. State Key Laboratory of Bioraacromolecules Institute of Biophysics Chinese Academy of Sciences 100101 Beijing China 2. Life Science Center Peking University 100871 Beijing China

Two monoclonal antibodies (McAb2C9, McAb1E5) against staphylococcal nuclease R (SNase R) and its N terminal peptide fragments were prepared, purified and characterized. Further studies show that the intact enzyme SNase R and its seven N terminal peptide fragments differ in their interaction with McAb2C9. SNase R, SNR121, SNR102, SNR79 and SNR52 can bind to McAb2C9 readily, while fragments of SNR141, SNR135, SNR110 react with the antibody poorly. If this difference is due to diverse extent of exposure of the specific epitope in the fragments, it is suggested that the conformation of the peptide is subjected to continuous adjustments through chain elongation until the biologically active protein is formed. This result supports Tsou’s hypothesis of nascent peptide folding experimentally.

关键词： staphylococcal nuclease monoclonal antibody epitope protein folding.

Determining of antibiotic resistance profile in Staphylococcus aureus isolates

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Asian Pacific Journal of Tropical Medicine 2010年第9期3卷 734-737页

作者： Hossein Motamedi Hadis Mirzabeigi Tahere Shirali Department of Biology Faculty of ScienceShahid Chamran UniversityAhvazIran Student of Veterinary medicine Department of Clinical ScienceFaculty of Veterinary MedicineShahid Chamran UniversityAhvazIran

Objective:To determine the pattern of antibiotic resistance among Staphylococcus aureus(S. aureus) isolates from clinical specimens and to identify community-acquired methicillin-resistant Staphylococcus aureus(CA-MRSA) in specimens that have been collected from patients referring to one of the hospitals of ***:*** isolates from a hospital in Ahvaz were screened for resistance to various antibiotics including *** susceptibility of the isolates was determined by Kirby-Bauer disc diffusion *** MRSA was also treated with ethidium bromide to find the origin of ***:Among the bacterial isolates,all of 11 *** were resistant to methicillin and cefixime,2 were resistant to ciprofloxacin,6 were resistant to tetracycline and the reminder were sensitive or intermediate to other *** treated isolates were reminded resistant to methicillin and this suggested that the plasmid was not the origin of resistance in these ***:These results showed that infection due to MRSA is widespread in Ahvaz and with respect to the spread of vancomycin resistance among MRSA and appearance of overwhelming *** is necessary to identify continuously the profile of antibiotic resistance among *** isolates in other regions and finding appropriate antibiotic for infection control and eradication.

关键词： Staphylococcus aureus Antimicrobial resistance MRSA staphylococcal infections Ahvaz Community-acquired-MRSA

A Case of Erythema Nodosum Associated with Subareolar Abscess

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第4期2卷 263-264页

作者： Satoko Minakawa Yasushi Matsuzaki Daiki Rokunoke Eijiro Akasaka Koji Nakajima Takayuki Aizu Takahide Kaneko Hajime Nakano Kazutoshi Takashima Daisuke Sawamura Departments of Dermatology Hirosaki University Graduate School of Medicine Departments of Dermatology Hirosaki University Graduate School of Medicine Hirosaki Japan Departments of Surgery Hiro- saki University Graduate School of Medicine Hirosaki Japan

While erythema nodosum is a nodular, erythematous eruption predominantly affecting the extensor aspects of the legs, breast abscesses are the result of relatively common bacterial infections, and only 2 studies have reported an association between erythema nodosum and breast abscesses. Here, we report the case of a patient with repeated erythema nodosum associated with subareolar abscesses. The patient was a 34-year-old woman with painful erythematous nodules on her right shin, accompanied by an indurated lesion on her right breast 4 days before the onset of the nodules. Therefore, the patient underwent circumareolar incision, and consequently the painful erythematous nodules disappeared. However, after 39 days, the patient developed another tender, painful lesion in her right breast and painful erythematous nodules on her right shin. After another circumareolar incision, the painful erythematous nodules disappeared again. Therefore, we suggested a significant association between erythema nodosum and breast abscess in this patient. The most common underlying causative organism in breast abscess is Staphylococcus aureus;however, erythema nodosum has rarely been proven to be associated with staphylococcal infections. Therefore, the relationship between S. aureus and erythema nodosum is rather controversial. However, the resistance to the usual treatment methods and prolonged clinical course in our case suggest that the pathogenesis of erythema nodosum associated with breast abscesses might be different from that of the common form of erythema nodosum.

关键词： Erythema Nodosum Subareolar Abscess Breast staphylococcal Infections

链球菌G蛋白——一种新型、广谱的免疫检测剂

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医学研究生学报 1989年第1期2卷 68-70页

作者：王福安张太和金陵医院超微结构科

某些链球菌菌株的细胞壁中含有一种能与免疫球蛋白G(immunoglobulin,IgG)结合的蛋白质成分,称为IgG Fc段受体或链球菌G蛋白(streptococcal protein G)。近年来,此种蛋白质已被成功分离、纯化,并应用于免疫学研究。与经典的葡萄球菌A蛋白... 详细信息

某些链球菌菌株的细胞壁中含有一种能与免疫球蛋白G(immunoglobulin,IgG)结合的蛋白质成分,称为IgG Fc段受体或链球菌G蛋白(streptococcal protein G)。近年来,此种蛋白质已被成功分离、纯化,并应用于免疫学研究。与经典的葡萄球菌A蛋白(staphylococcal protein A,SPA)相比,链球菌G蛋白(以下简称SPG)更易与IgG发生反应,亲和力强,结合谱广,已受到密切的关注。目前,国内尚未见有关SPG研究的综述文章及实验报道。现将国外SPG研究的新进展介绍如下。

关键词：免疫检测 IgG 免疫学研究 staphylococcal 实验报道 immunoglobulin 阳性菌株胶体金标记免疫分析链球菌属

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葡萄球菌性烫伤样皮肤综合征的护理体会

求医问药（下半月） 2013年第8期11卷 231-232页

作者：余婷玉广西中医学院广西南宁530001

葡萄球菌性烫伤样皮肤综合征(staphylococcal scalded skin syndrome,SSSS)由凝固酶阳性的噬菌体第II组金黄色葡萄球菌产生的表皮松解毒素所致,是一种以全身泛发性红斑、松弛性大疱和大片表皮剥脱呈烫伤样外观为特征的严重感染性皮肤病... 详细信息

葡萄球菌性烫伤样皮肤综合征(staphylococcal scalded skin syndrome,SSSS)由凝固酶阳性的噬菌体第II组金黄色葡萄球菌产生的表皮松解毒素所致,是一种以全身泛发性红斑、松弛性大疱和大片表皮剥脱呈烫伤样外观为特征的严重感染性皮肤病[1]。该病好发于新生儿及婴幼儿,起病急,病情变化快,易并发败血症、支气管肺炎等,病死率高。由于该病的特殊性,其预后与护理直接相关。

关键词：表皮松解凝固酶阳性泛发性红斑大片表皮剥脱松弛性大疱金黄色葡萄球菌并发败血症感染性皮肤病 staphylococcal 支气管肺炎