检索结果-南通市图书馆

Preliminary Study of Effects of Centrifugation of stallion.Semen on Motility and Pro-AKAP4 Concentration over 72 Hours Post-collection

引用

Journal of Agricultural Science and Technology(A) 2022年第2期12卷 65-72页

作者： Mikaila Zi Yue Smith Anna Dilger Erin Bryan Giana Bytnar Suhna Choi Kelly Cook Kevin Kline Department of Animal Sciences University of Illinois at Urbana Champaign1207 West Gregory DriveUrbanaIL 61801USA

The study aimed to investigate the effects of centrifugation of stallion.semen on progressive sperm motility over a 72-hour period;and determine if the protein marker,precursor of A-kinase anchor protein 4(pro-AKAP4),may be maintained at a higher level over time when compared to non-centrifuged ***-AKAP4 has been studied for its function in stabilizing the structure of spermatozoa and its use as a marker for fertilizing capacity of *** collections were obtained from four fertile,commercially used Standardbred stallion. collected three times *** samples were diluted to a final concentration of 50 million total cells per *** extended aliquot of semen from each collection was used for the non-centrifuged treatment,and one extended aliquot of semen was centrifuged at 750×g for 10 *** supernatant from the centrifuged sample was then removed with a 10 mL plastic transfer pipette,and the spermatozoa cell pellet was suspended in fresh extender to a final concentration of 50 million total cells per *** centrifuged and non-centrifuged samples from each collection were stored in Equine Express II stallion.semen shipping containers for 72 h and fresh ice packs were added to each container every 24 *** progressive sperm motility and levels of pro-AKAP4 were determined at times 0,12,24,48 and 72 h after *** were found between stallion.,times and treatments with the main finding being that pro-AKAP4 concentration in centrifuged samples was maintained at a much higher level over the 72-hour period studied.

关键词： Centrifuged extender pro-AKAP4 spermatozoa stallion.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：