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Laminaria gametophyte clone culture and its application in sporeling cultivation

Acta Oceanologica Sinica 2000年第2期19卷 89-95页

作者： Zhou Zhigang1 Li Dapeng2 Wu Chaoyuan2 Liu Haihang2 (1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Prasent address: College of Fishery Science, Shanghai Fisheries University, 334 Jungong Road, Shangha 20 College of Fishery Science Shanghai Fisheries University Shanghai 200090 334 Jungong Road China

In China, the total annual production of cultured Laminaria japonica reached half million tons in dry weight there few years. The routine sporeling culture technique conducted in the greenhouse took at least three and half months. In such a case, sometimes the sporelings died within a few days due to destructive diseases. In Order to overcome the mentioned problems, a new sporeling culture technique, the clone technique, is developed. The method includes three stead: (1) Gametophyte clone culture. The spores and the gametophytes are cloned in flasks under favorable environments. (2) sporeling cul ture. Male and female clones are crushed and spread onto a frame to allow the gametophytes to attach to the substrata. The frames are cultured in tanks, and the sporophytes reach 1 cm in length within one and a half months. (3) Outgrowing of the plant. The frames are put in the open sea when seawater temperature decreased to 20℃. After one month, the sporelings are large enough to be transplanted. It is concluded that the clone technique has the following advantages: (1)Large amount of clones can be produced in a short period of time. (2) Clone seeding method makes it free from the biological rhythm, one can seed the plant anytime all the year round. (3) It takes only one and a half months to complete the process of sporeling cultivation in the greenhouse. At present, this technique is used in the breeding of new strains of Laminaria.

关键词： Clone culture gametophyte Laminaria cultivation nitrate effect phosphate effect sporeling sporophyte

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New phenomenon in early development of sporelings in Gracilaria asiatica Chang et Xia(Gracilariaceae,Rhodophyta)

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Chinese Journal of Oceanology and Limnology 2006年第4期24卷 364-369页

作者：赵凤娟王爱华刘吉东段德麟 Institute of Oceanology Chinese Academy of Sciences

Study on the early development of sporelings from carpospores of Gracilaria asiatica Chang et Xia was conducted indoors under controlled culture conditions. Besides normal development of sporelings, a new developmental phenomenon of filamentous frond was observed. It was composed of one or two rows of cells, and took place from the outmost brim of the basal disc. During the early disc stage of germinated carpospores, one or two filamentous fronds formed on about 10% basal discs. Simul-taneously, young fronds began to arch slightly from the centers of single and coalescent discs; lately more filamentous fronds up to 80% appeared on the brims of basal discs. Meanwhile one or more upright fronds protuberated on the basal discs. Generally, filamentous fronds exhibited in self-existence or co-existence forms with normal young sporelings on the same basal disc, and single cell detached from filamentous fronds developed into a new filamentous frond. This new phenomenon exhibited a unique differentiation pathway during the early development of G. asiatica, which would be potential for the ap-plication in artificial sporelings nursery.

关键词： filamentous fronds basal disc sporeling early development Gracilaria asiatica

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STUDIES ON INTERCROPPING OF PORPHYRA HAITANENSIS *** ET *** WITH BAY SCALLOP ARGOPECTEN IRRADIANS LAMARCK

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Chinese Journal of Oceanology and Limnology 1993年第1期11卷 16-20页

作者：郑宝福李世英管明金 Institute of Oceanology Academia Sinica Qingdao 266071 Jiaonan Seafood Manufactory Jiaonan 266426

Results of the experiment of northward transplantation of Porphyra haitanensis showed thatintercrop of P. haitanensis with Bay Scallop is feasible because the growth duration of both of them is about the same in August - *** use of cultivating area, lowering its cost and increasing its product per unit area as well as improving the ecological envronment are realized during intercrop.

关键词： intercrop P. haitanensis Bay Scallop conchocelis sporeling

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