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Electrical fields and the promotion of endogenous angiogenesis in a rat spinal cord injury model

Neural Regeneration Research 2010年第14期5卷 1091-1095页

作者： Yang Shao Bo Xiao Haihan Ma Jie Gao Qidong Yang Min Zhao Department of Neurology Xiangya Hospital Central South University Changsha 410008 Hunan Province China State Key Laboratory of Trauma Burns and Combined Injury Institute of Surgery Research Daping Hospital Third Military Medical University Chongqing 400042 China School of Medical Sciences University of Aberdeen Aberdeen AB25 2ZD UK

BACKGROUND： Previous studies have shown that direct current electrical fields affect development and growth of human microvascular endothelial cells, but the role of electrical fields on promoting angiogenesis in tissues following spinal cord injury remains poorly understood. OBJECTIVE： To determine the effects of electrical fields on angiogenesis and spinal cord repair following traumatic spinal cord injury in rats. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Chongqing Key Laboratory of Neurology, Affiliated Hospital of Chongqing Medical University, China from September 2007 to August 2008. MATERIALS： Hydrogen blood flow detector （Soochow University Medical Instrument, China）, Power Lab System （AD Instruments, Colorado Springs, CO, USA） and mouse anti-vascular endothelial growth factor （VEGF） monoclonal antibody （Sigma-Aldrich, St. Louis, MO, USA） were used in this study. METHODS： A total of 60 healthy, adult, Sprague Dawley rats were equally and randomly assigned to sham-surgery, model, and electrical field groups. The Allen＇s weight-drop method was used to induce complete spinal cord injury in the model and electrical field groups. Rats in the electrical field group were implanted with silver needles and electrical fields （350 V/m） were applied following traumatic injury. MAIN OUTCOME MEASURES： Latency of somatosensory-evoked potential was detected and spinal cord blood flow was measured by hydrogen blood flow detector. Microvascular density was determined by histological analysis. VEGF expression in the spinal cord was observed by immunohistochemical staining. RESULTS： Recovery of spinal cord blood flow was significantly increased in the electrical field group （at 1, 2, 4, 8, and 24 days after injury） compared with the model group （P 〈 0.05 or P 〈 0.01）. Latency of P1 waves in somatosensory-evoked potential of electrical field group （at 1,2, 4, 8, and 24 days after injury） was significantly shorter than the

关键词： angiogenesis electric fields spinal cord injury spinal cord blood flow vascular endothelial growth factor neural regeneration

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