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sperm membrane modulation by Sapindus mukorossi during sperm maturation

Asian Journal of Andrology 2002年第3期4卷 233-235页

作者： Manish Nivsarkar Neeta Shrivastava Manoj Patel Harish Padh Cherian Bapu B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre Thaltej-Gandhinagar Highway Thaltej Ahmedabad-380 054 Gujarat IndiaB.V. Patel Pharmaceutical Education and Research Development (PERD) Centre Thaltej-Gandhinagar Highway Thaltej Ahmedabad-380 054 Gujarat IndiaB.V. Patel Pharmaceutical Education and Research Development (PERD) Centre Thaltej-Gandhinagar Highway Thaltej Ahmedabad-380 054 Gujarat IndiaB.V. Patel Pharmaceutical Education and Research Development (PERD) Centre Thaltej-Gandhinagar Highway Thaltej Ahmedabad-380 054 Gujarat IndiaB.V. Patel Pharmaceutical Education and Research Development (PERD) Centre Thaltej-Gandhinagar Highway Thaltej Ahmedabad-380 054 Gujarat India

Aim: To observe the alterations in the biochemical and biophysical changes in the sperm membrane during sperm maturation in male rats treated with the water extract of the fruit pericarp of S. mukorossi. Methods: Adult male Sprague-Dawley rats were gavaged the aqueous extract of the fruit pericarp of S. mukorossi at a dose of 50 mg/kg/d for 45 days. On day 46, the sperm parameters were observed in different sections of the epididymis and the sperm superoxide dismutase and the lipid peroxidation was determined and compared with the controls. The testis and epididymis were routinely prepared for histological examination under the light microscope. Results: No significant differences in the sperm number and morphology were observed between the control and treated groups. However, a significant inhibition (Psperm motility in the caput, corpus and cauda regions of the epididymis was seen in the treated group. No significant histopathological changes were found in the testis and epididymis. The important finding was that in the treated animals, the spermatozoa showed an abnormal distribution of the superoxide dismutase activity, being minimum in the caput and maximum in the corpus, which was just opposite to that of the controls. Conclusion: The study provides a unique observation where the plant extract alters the sperm membrane physiology without change the testicular and epididymal morphology.

关键词： Sapindus mukorossi sperm membrane contraceptive sperm motility

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Influence of enterococci on human sperm membrane in vitro

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Asian Journal of Andrology 2007年第1期9卷 77-81页

作者： Hua Qiang Ming-Sen Jiang Jian-Yin Lin Wei-Min He Department of Pathogenic Biology Etiology School of Medicine Wuhan University Wuhan 430071 China Department of Etiology 3Department of Anatomy Histology and Embryology Fujian Medical University Fuzhou 350004 China 不详　

Aim： To study the influence of enterococci on human sperm membrane in vitro. Methods： Ejaculated human sperm were artificially infected with β-hemolytic or non-β-hemolytic enterococci at the bacteria： sperm ratio of 50：1 at 37℃. sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling （HOS） test and electron microscopy. Results： sperm infected with β-hemolytic enterococci had lower HOS scores compared with non-β-hemolytic strains or uninfected control （P 〈 0.01）. The HOS test scores of sperm infected with β-hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non-β-hemolytic strains showed no significant difference in swelling rate, compared with the control group （P 〉 0.05）. It was shown by electron microscopy that β-hemolytic enterococci caused significant rupture of human sperm membrane. Conclusion： β-hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.

关键词： enterococci sperm sperm membrane hypoosmotic swelling test ultrastructure

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Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第6期12卷 448-454页

作者： Yi QIU Lei-guang WANG Yi-fang JIA Dan-tong YANG Mei-hua ZHANG Yan-ping ZHANG Li-hong ZHANG Ling GAI Key Laboratory for Improving Birth Outcome Technique Shandong Provincial Institute of Science and Technology for Family Planning Jinan 250002 China

The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifo/ia Willd （PTW） in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity （Sander-Cramer assay） of the extract from the PTW root. The hypo-osmotic swelling （HOS） test and the eosin Y （EY） staining were used to detect the integrity of sperm membrane and vitality. The sperm chromatin dispersion （SCD） test was performed to determine sperm DNA integrity. N-9 was used as a reference standard and semen added to physiological saline was used as the control. Semen samples were donated by 42 healthy fertile men. The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml; at the concentration of 5.0 mg/ml, spermatozoa were immobilized in （39.5±3.2） s. In the groups of the crude extract from the root of PTW and N-9 solution the rate of the normal HOS （tails swollen） and the white head （unstained） was 0%, and the rate of the abnormal HOS （tails unswollen） and red head （stained） was 100%. sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution. The sperm revival test did not show any spermatozoa that recovered their motilities. The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity.

关键词： Polygala tenuifofia Willd Crude extract spermicidal effect in vitro sperm membrane DNA integrity

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