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Magnetic-activated cell sorting of nonapoptotic spermatozoa with a high dna fragmentation index improves the live birth rate and decreases transfer cycles of IVF/ICSI

引用

Asian Journal of Andrology 2022年第4期24卷 367-372页

作者： Jie Mei Lin-Jun Chen Xin-Xin Zhu Wen Yu Qing-Qiang Gao Hai-Xiang Sun Li-Jun Ding Jun-Xia Wang Center for Reproductive Medicine Department of Obstetrics and GynecologyNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Center for Molecular Reproductive Medicine Nanjing UniversityNanjing 210008China Department of Andrology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Clinical Center for Stem Cell Research The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China

The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting(MACS)in sperm preparation for male subjects with a sperm dna fragmentation index(DFI)≥30%.A total of 86 patients who had undergone their first long-term long protocol were selected.The protocol involved in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI)cycles,and the patients were divided into the MACS or control groups.The MACS group included sperm samples analyzed with MACS that were combined with density gradient centrifugation(DGC)and the swim-up(SU)technique(n=39),and the control group included sperm samples prepared using standard techniques(DGC and SU;n=41).No differences were noted with regard to basic clinical characteristics,number of oocytes retrieved,normal fertilization rate,cleavage rate,or transplantable embryo rate between the two groups in IVF/ICSI.In addition,the clinical pregnancy and implantation rates of the first embryo transfer cycles indicated no significant differences between the two groups.However,there was a tendency to improve the live birth rate(LBR)of the first embryo transfer cycle(63.2%vs 53.9%)and the cumulative LBR(79.5%vs 70.7%)in the MACS group compared with the control group.Moreover,the number of transferred embryos(mean±standard deviation[s.d.]:1.7±0.7 vs 2.3±1.6)and the transfer number of each retrieved cycle(mean±s.d.:1.2±0.5 vs 1.6±0.8)were significantly lower in the MACS group than those in the control group.Thus,the selection of nonapoptotic spermatozoa by MACS for higher sperm DFI could improve assisted reproductive clinical outcomes.

关键词： cumulative live birth rate fertility rate intracytoplasmic sperm injection sperm dna fragmentation index sperm dna integrity

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