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Identification of novel QTL associated with soybean isoflavone content

The Crop Journal 2018年第3期6卷 244-252页

作者： Ruili Pei Jingying Zhang Ling Tian Shengrui Zhang Fenxia Han Shurong Yan Lianzheng Wang Bin Li Junming Sun The National Engineering Laboratory for Crop Molecular Breeding MOA Key Laboratory of Soybean Biology (Beijing)Institute of Crop SciencesChinese Academy of Agricultural Sciences

Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, highresolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing(SLAF-seq) with a recombinant inbred line(RIL) population(F5:7) derived from a cross between two cultivated soybean varieties, Luheidou 2(LHD2) and Nanhuizao(NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%–21.2% of phenotypic variation.Of these QTL, four novel stable QTL(qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis(Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%–15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs.

关键词： Soybean(Glycine max L.Merrill) QTL mapping Isoflavones specific-locus amplified fragment sequencing(SLAF-seq)

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Construction of the first high-density genetic map for growth related QTL analysis in Ancherythroculter nigrocauda

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Journal of Oceanology and Limnology 2021年第3期39卷 1118-1130页

作者： Yanhong SUN Pei LI Guiying WANG Renli SUN Jian CHEN Qi ZHOU Jingou TONG Qing LI Fisheries Research Institute Wuhan Academy of Agricultural SciencesWuhan 430207China Wuhan Xianfeng Aquaculture Technology Co.Ltd. Wuhan 430207China Wuhan Academy of Agricultural Sciences Wuhan 430207China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China

Ancherythroculter nigrocauda is a fish endemic to the upper areas of the Changjiang(Yangtze)River in China.Quantitative trait locus(QTL)mapping is a powerful tool to identify potential genes affecting traits of economic importance in domestic animals.In this study,a high-density genetic map was constructed with 5901 single nucleotide polymorphism(SNP)makers by sequencing 92 individual fish from a F1 family using the specific-locus amplified fragment sequencing approach.Initially,48 QTLs for total length,body length,body height,and body weight were identified according to the high density of the genetic map with 24 LGs,a total length of 3839.4 cM,and marker spacing of about 0.82 cM.These QTLs explained 27.1%-49.9%of phenotypic variance.The results of this study suggest that major QTLs are responsible for the growth of A.nigrocauda,and these are potentially useful in comparative genomics research,genome assembly,and marker-assisted breeding programs for this species.

关键词： Ancherythroculter nigrocauda specific-locus amplified fragment high-density genetic map quantitative trait locus

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