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Journal of Integrative Agriculture 2019年第5期18卷 1042-1049页

作者： WANG Yu-sheng TIAN Hu WAN Fang-hao ZHANG Gui-fen State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop Ministry of Agriculture/Institute of Plant Protection Chinese Academy of Agricultural Sciences Caofeidian Sub-Center of Hebei Entry-Exit Inspection and Quarantine Technical Center

The globally invasive cassava mealybug Phenacoccus manihoti Matile-Ferrero is a pernicious pest of cassava,and its recent introduction into Asia has raised considerable alarm.To slow or prevent further invasion,an accurate,simple,and developmental-stage-independent detection method for P.manihoti is required.In the present study,a PCR method based on a species-specific mitochondrial DNA cytochrome oxidase I(SS-COI)marker was developed for rapid identification of P.manihoti.One pair of SS-COI primers(PMSSZW-1F and PMSSZW-1R)was designed based on sequence variations in the COI gene among P.manihoti and related mealybug species.specificity of the primer pair was validated on 21 closely related species.Sensitivity tests were performed on four immature developmental stages and female adults.Efficacy tests demonstrated that at the relatively low concentration of(135.2±14.7)pgresuspended DNA,the specific fragment was detected in all replicates.Furthermore,the SS-COI primer pair was assayed on three populations of P.manihoti from major exporting countries of cassava.The PCR assay was proved to be a rapid,simple,and reliable molecular measure for the identification of P.manihoti.This tool will be useful for quarantine,monitoring,and management of this invasive pest.

关键词： Phenacoccus manihoti cassava mealybug invasive pest molecular identification species-specific COI primers

Identification of various Biomphalaria alexandrina strains collected from five Egyptian governorates using RAPD and species-specific PCR techniques

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American Journal of Molecular Biology 2011年第1期1卷 17-25页

作者： A. M. Mohamed A. T. Sharaf El-Din A. H. Mohamed M. R. Habib Menoufiya University

The first generation of Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta) were sub-jected to species-specific PCR assays and the results showed that snails collected from the field were B. alexandrina, and there was no evidence for the pres-ence of B. glabrata. The snails were subjected also to RAPD- PCR technique. The results showed that dif-ferent fingerprints with each B. alexandrina strain were produced with varying numbers of bands rang-ing in size from 123.6 to 796.6 bp depending on the snail strain and the primer used. Many specific bands were obtained with the four primers in each strain. Primer OPA-1 amplified the highest number of spe-cific bands (26 bands) and gave the highest poly-morphism among the primers used (100% polymor-phism). The estimated similarity coefficients among B. alexandrina strains based on the RAPD-PCR pro-files ranged from 0.56 to 0.72. The highest similarity coefficient (0.72) was recorded between the strains of Ismailia and Kafr El-Sheikh, while the lowest coeffi-cient (0.56) was reported between the strains of SPSC and Ismailia.

关键词： Biomphalaria Alexandrina Egypt Rapd species-specific Pcr

Multi-locus identification of Psilocybe cubensis by high-resolution melting(HRM)

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Forensic Sciences Research 2022年第3期7卷 490-497页

作者： Xiaochun Zhang Huan Yu Ziwei Wang Qi Yang Ruocheng Xia Yiling Qu Ruiyang Tao Yan Shi Ping Xiang Suhua Zhang Chengtao Li Department of Forensic Science Medical School of Soochow UniversitySuzhouChina Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic SciencesMinistry of JusticeShanghaiChina Department of Forensic Medicine School of Basic Medical ScienceWenzhou Medical UniversityWenzhouChina Institute of Forensic Medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina

Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin,psilocin and ibotenic acid,etc.The mushrooms containing hallucinogenic components are various,widely distributed and lack of standard to define,which made a great challenge to identification.Traditional identification methods,such as morphology and toxicology analysis,showed shortcomings in old or processed samples,while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability of DNA.In this paper,four primer sets are designed to target Psilocybe cubensis DNA for increasing resolution of present identification method,and the target markers include largest subunit of RNA polymerase II(marked as PC-R1),psilocybin-related phosphotransferase gene(marked as PC-PT),glyceraldehyde 3-phosphate dehydrogenase(marked as PC-3)and translation EF1α(marked as PC-EF).Real-time PCR with high-resolution melting(HRM)assay were used for the differentiation of the fragments amplified by these primer sets,which were tested for specificity,reproducibility,sensitivity,mixture analysis and multiplex PCR.It was shown that the melting temperatures of PC-R1,PC-PT,PC-3 and PC-EF of P.cubensis were(87.93±0.12)℃,(82.21±0.14)℃,(79.72±0.12)℃ and(80.11±0.19)℃ in our kinds of independent experiments.Significant HRM characteristic can be shown with a low concentration of 62.5pg/µL DNA sample,and P.cubensis could be detected in mixtures with Homo sapiens or Cannabis sativa.In summary,the method of HRM analysis can quickly and specifically distinguish P.cubensis from other species,which could be utilized for forensic science,medical diagnosis and drug trafficking cases.

关键词： Forensic sciences forensic genetics polymerase chain reaction(PCR) high-resolution melting(HRM) Psilocybe cubensis multi-locus species-specific

Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC-MS/MS analyses

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Insect Science 2021年第5期28卷 1369-1381页

作者： Hai-Jian Huang Zhuang-Xin Ye Gang Lu Chuan-Xi Zhang Jian-Ping Chen Jun-Min Li State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingboZhejiang315211.China

The whitefly Bemisia tabaci is a notorious agricultural pest of many crops worldwide.Although it is thought that B.tabaci secretes saliva into the host plant to counter plant defenses,knowledge on the whitefly salivary proteome is limited.Here,we characterized the gene/protein repertoires of B.tabaci salivary glands and secreted saliva by transcriptomic and liquid chromatography tandem mass spectroscopy analyses.A total of 698 salivary gland-enriched unigenes and 171 salivary proteins were identified.Comparative analysis between the B.tabaci salivary proteins and those of different arthropod species revealed numerous similarities in proteins associated with binding,hydrolysis,and oxidation-reduction,which demonstrates a degree of conservation across herbivorous saliva.There were 74 proteins only identified in B.tabaci saliva,of which 34 were B.tabaci-specific.In addition,13 salivary proteins,of which I1 were B.tabaci-specific,were differentially regulated when B.tabaci fed on different hosts.Our results provide a good resource for future functional studies of whitefly salivary effectors,and might be useful in pest management.

关键词： Bemisia tabaci proteome salivary protein species-specific transcriptome

Supplemental blue light increases growth and quality of greenhouse pak choi depending on cultivar and supplemental light intensity

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Journal of Integrative Agriculture 2018年第10期17卷 2245-2256页

作者： ZHENG Yin-jian ZHANG Yi-ting LIU Hou-cheng LI Ya-min LIU Ying-liang HAO Yan-wei LEI Bing-fu College of Horticulture South China Agricultural University College of Materials and Energy South China Agricultural University

To evaluate the supplementary blue light intensity on growth and health-promoting compounds in pak choi（Brassica campestris ssp.chinensis var.communis）,four blue light intensity treatments（T0,T50,T100 and T150 indicate 0,50,100,and 150μmol m^（-2） s^（-1）,respectively）were applied 10 days before harvest under greenhouse conditions.Both of cultivars（greenand red-leaf pak choi）under T50 had the highest yield,content of chlorophyll and sugars.With light intensity increasing,antioxidant compounds（vitamin C and carotenoids）significantly increased,while nitrate content showed an opposite trend.The health-promoting compounds（phenolics,flavonoids,anthocyanins,and glucosinolates）were significantly higher under supplementary light treatment than T0,so as the antioxidant capacity（2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power）.The species-specific differences in photosynthetic pigment and health-promoting compounds was found in green-and red-leaf pak choi.T50 treatment could be used for yield improvement,whereas T100 treatment could be applied for quality improvement.Results showed that blue light intensity can regulate the accumulation of biomass,morphology and health-promoting compounds in pak choi under greenhouse conditions.

关键词： blue light antioxidant compounds health-promoting compounds species-specific pak choi

Molecular interaction of zp3 to zp3r reveals a cross-species fertilization mechanism

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Asian pacific Journal of Reproduction 2017年第3期6卷 116-120页

作者： Reni Kurniati Didik Huswo Utomo Sri Rahayu Widodo Sutiman Bambang Sumitro Departement of Biology Faculty of Mathematics and Natural SciencesMulawarman UniversitySamarindaIndonesia Departement of Biology Faculty of Mathematics and Natural SciencesBrawijaya UniversityMalangIndonesia

Objective: To evaluate the role of ZP3R in the species-specific fertilization mechanism. Methods:ZP3/ZP3R protein sequences ofMus musculus,Rattus norvegicus, andCavia porcellus were downloaded from UNIPROT. Percentage of amino acids that was calculated by using the SIAS program. Protein sequences modeled was established by using the Modeller 9.14 program and glycosylation of the ZP3 using GlyProt program. Docking simulation of the ZP3R-ZP3 was performed between the same species and different species with PatchDock program.Results:Comparison of the ZP3R and ZP3 structure between species showed that ZP3 in these three species was more similar than ZP3R. Docking simulations of protein showed that changes in the pattern of the ZP3-ZP3R domain for interaction on cross-species compared to the same species. Changes in the pattern of binding ZP3R-ZP3 made sperm-egg binding was not functional and could inhibit cross-fertilization.Conclusions: ZP3R-ZP3 interaction is species-specific, and the role of ZP3R is greater than ZP3 in determining the species-specific recognition stage and sperm-egg binding.

关键词： ZP3R species-specific Fertilization

Chaffinch (Fringilla coelebsL.) song in populations of the East Europe

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Open Journal of Animal Sciences 2012年第4期2卷 211-216页

作者： Olesya Anatolyevna Astakhova Department of Vertebrate Zoology M. V. Lomonosov Biology Faculty Moscow State University Moscow Russia

At studying of song organizations of chaffinch (Fringilla coelebs L.) are found out unique parities of song types in different local populations of a species-specific area. Thus in different areas of the East Europe are forming original (different) song cultures of chaffinch—the certain set of song types including so-called “dialect song forms”. The complex interrelation of geographical variability of chaffinch song in many respects gives a support at an evolutionary view in the given aspect.

关键词： species-specific Song of Chaffinch Different Song Types Song Cultures Degree of Prevalence of Song Types in Populations

Degree of prevalence of different song types of chaffinch (Fringilla coelebsL.) in populations of the European Russia

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Open Journal of Animal Sciences 2014年第1期4卷 31-37页

作者： Olesya Anatolyevna Astakhova Department of Vertebrate Zoology M. V. Lomonosov Biology Faculty Moscow State University Russia Moscow

When studying song organizations of chaffinch (Fringilla coelebs L.), we find the unique typological parities (ratio) of songs in different local populations of a species-specific area. The degree of prevalence of different chaffinch song types can’t correspond to percentage proceeding from their general (common) number in a population. Thus different areas of Russia are forming original (different) song cultures of chaffinch, consisting of the certain set and a parity (ratio) of song types, frequently including the so-called dialect song forms, that have been observed. The complex interrelation of geographical variability and structural variability of chaffinch song in many respects gives a support in an evolutionary view from the given aspect.

关键词： species-specific Song of Chaffinch Different Song Types Song Cultures Degree of Prevalence of Song Types in Populations

Vocal Variability of Chaffinch Song (Fringilla coelebsL.) as a Condition of Cultural Evolution in Local Populations

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Open Journal of Animal Sciences 2014年第2期4卷 59-69页

作者： Olesya Anatolyevna Astakhova Department of Vertebrate Zoology Faculty of Biology Moscow State University of M. V. Lomonosov Moscow Russia

There are such characteristics of a matter of the nature, as variability and stability (tradition, norm). Probably, these opposites process as if other qualities of different forms of energy also create “movement” development. Thus the given properties of a matter of the nature can be considered at different levels of its organization. The singing of many passerine birds is incontrovertible feature of their life which determines and builds a reproductive cycle. By studying song repertoire of many sparrow species in details, it was revealed that the individual has not only one, but some types of songs. Thus spring singing represents the multifunctional phenomenon and can bear (carry) various values. The singing is not only a means of attracting females, but also a means of intimidation of the contender and delimitation of nested territory. Variants or types of species-specific song are individually various and distributed between individuals of a population. The complex interrelation of geographical variability of chaffinch song in many respects gives a support at an evolutionary view in the given aspect.

关键词： species-specific Song of Chaffinch Different Song Types Song Cultures Song Evolution

Differences in Amino Acid Composition between αand βStructural Classes of Proteins

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Journal of Biomedical Science and Engineering 2014年第11期7卷 890-918页

作者： Hiroshi Nakashima Yuka Saitou Nachi Usuki Department of Clinical Laboratory Science Graduate Course of Medical Science and Technology School of Health Sciences Kanazawa University Kanazawa Japan

The amino acid composition of α and β structural class of proteins from five species, Escherichia coli, Thermotoga maritima, Thermus thermophilus, yeast, and humans were investigated. Amino acid residues of proteins were classified into interior or surface residues based on the relative accessible surface area. The hydrophobic Leu, Ala, Val, and Ile residues were rich in interior residues, and hydrophilic Glu, Lys, Asp, and Arg were rich in surface residues both in α and β proteins. The amino acid composition of α proteins was different from that of β proteins in five species, and the difference was derived from the different contents of their interior residues between α and β proteins. α-helix content of α proteins was rich in interior residues than surface ones. Similarly, β-sheet content of β proteins was rich in interior residues than surface ones. The content of Leu residues was very high, approximately 20%, in interior residues of α proteins. This result suggested that the Leu residue plays an important role in the folding of α proteins.

关键词： Amino Acid Composition α and β Proteins Interior/Surface Residue Protein Domains species-specific Amino Acid Composition