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Molecular Identification of Dried Shellfish Products Sold on the Market Using DNA Barcoding

Journal of Ocean University of China 2021年第4期20卷 931-938页

作者： SUN Shao’e ZHANG Xiaojie KONG Lingfeng LI Qi Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The dried shellfish products with rich nutrients and low-calorie content are favorite food in China,especially in coastal ***,the species of dried shellfish products in the market are usually unknown,as the taxonomic features were removed during the production *** study described the application of DNA barcoding technique to the identification of 100 dried shellfish(scallop,squid,octopus and cuttlefish)products in *** were authenticated by comparing mitochondrial cytochrome oxidase subunit I(COI)gene and 16S ribosomal RNA(16S rRNA)gene sequences with public reference taxonomic *** results showed that all the 100 products can be identified at species *** four scallop adductor products were processed using the bay scallop,Argopecten irradians,and one was from Portuguese oyster,Crassostrea *** the 27 squid,2 cuttlefish and 6 octopus products were produced by the Jumbo flying squid,Dosidicus *** neighbour-joining tree is in agreement with the results of DNA barcoding *** 64 scallop samples formed one *** cluster,leaving Sca65 clustered with the reference oyster sequence ***(MH997922).All the 35 cephalopod(squid,octopus and cuttlefish)samples formed a *** *** investigation revealed a low variety of dried shellfish products sold on the market,and highlighted the high rate of mislabeling and species *** present work provides a practical method for tracing and authenticating shellfish products.

关键词： dried shellfish product DNA barcoding species identification mislabeling species substitution

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