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R_(SC3)K of soybean cv. Kefeng No.1 confers resistance to soybean mosaic virus by interacting with the viral protein P3

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Journal of Integrative Plant Biology 2023年第3期65卷 838-853页

作者： Tongtong Jin Jinlong Yin Tao Wang Song Xue Bowen Li Tingxuan Zong Yunhua Yang Hui Liu Mengzhuo Liu Kai Xu Liqun Wang Guangnan Xing Haijian Zhi Kai Li National Key L aboratory for Crop Genetics and Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of AgricultureNational Center for Soybean ImprovementNanjing Agricultural UniversityNanjing 210095China Jiangsu Key Laboratory for Microbes and Functional Genomics College of Life SciencesNanjing Normal UniversityNanjing 210023China

soybean mosaic virus(SMV) is one of the most devastating viral pathogens of soybean(Glycine max(L.) Merr). In total, 22 Chinese SMV strains(SC1–SC22) have been classified based on the responses of 10 soybean cultivars to these pathogens. However, although several SMVresistance loci in soybean have been identified, no gene conferring SMV resistance in the resistant soybean cultivar(cv.) Kefeng No.1 has been cloned and verified. Here, using F_(2)-derived F_(3)(F_(2:3)) and recombinant inbred line(RIL) populations from a cross between Kefeng No.1 and susceptible soybean cv. Nannong 1138-2, we localized the gene in Kefeng No.1 that mediated resistance to SMV-SC3 strain to a 90-kb interval on chromosome 2. To study the functions of candidate genes in this interval, we performed Bean pod mottle virus(BPMV)-induced gene silencing(VIGS). We identified a recombinant gene(which we named R_(SC3)K) harboring an internal deletion of a genomic DNA fragment partially flanking the LOC100526921 and LOC100812666 reference genes as the SMV-SC3 resistance gene.By shuffling genes between infectious SMV DNA clones based on the avirulent isolate SC3 and virulent isolate 1129, we determined that the viral protein P3 is the avirulence determinant mediating SMV-SC3 resistance on Kefeng No.1. P3 interacts with RNase proteins encoded by R_(SC3)K, LOC100526921, and LOC100812666. The recombinant R_(SC3)K conveys much higher anti-SMV activity than LOC100526921 and LOC100812666, although those two genes also encode proteins that inhibit SMV accumulation, as revealed by gene silencing in a susceptible cultivar and by overexpression in Nicotiana benthamiana. These findings demonstrate that R_(SC3)K mediates the resistance of Kefeng No.1 to SMV-SC3 and that SMV resistance of soybean is determined by the antiviral activity of RNase proteins.

关键词： recombination resistance gene soybean mosaic virus virulence determinant

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GmTOC1b negatively regulates resistance to soybean mosaic virus

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The Crop Journal 2023年第6期11卷 1762-1773页

作者： Yuhang Zhang Haiping Du Tiantian Zhao Chunmei Liao Tu Feng Jun Qin Baohui Liu Fanjiang Kong Zhijun Che Liyu Chen Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina Hebei Laboratory of Crop Genetics and Breeding National Soybean Improvement Center Shijiazhuang Sub-CenterHuang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of SoybeanMinistry of Agriculture and Rural AffairsInstitute of Cereal and Oil CropsHebei Academy of Agricultural and Forestry SciencesShijiazhuang 050035HebeiChina School of Ecological Engineering Guizhou University of Engineering ScienceBijie 551700GuizhouChina School of Agriculture Ningxia UniversityYinchuan 750021NingxiaChina College of Life Science Hebei Normal UniversityShijiazhuang 050024HebeiChina

soybean(Glycine max)is a major oil and feed crop worldwide.soybean mosaic virus(SMV)is a globally occurring disease that severely reduces the yield and quality of soybean.Here,we characterized the role of the clock gene TIMING OF CAB EXPRESSION 1b(GmTOC1b)in the resistance of soybean to SMV.Homozygous Gmtoc1b mutants exhibited increased tolerance to SMV strain SC3 due to the activation of programmed cell death triggered by a hypersensitive response.Transcriptome deep sequencing and RT-qPCR analysis suggested that GmTOC1b likely regulates the expression of target genes involved in the salicylic acid(SA)signaling pathway.GmTOC1b binds to the promoter of GmWRKY40,which encodes a protein that activates the expression of SA-mediated defense-related genes.Moreover,we revealed that the GmTOC1bH1 haplotype,which confers increased tolerance to SMV,was artificially selected in improved cultivars from the Northern and Huang-Huai regions of China.Our results therefore identify a previously unknown SMV resistance component that could be deployed in the molecular breeding of soybean to enhance SMV resistance.

关键词： GmTOC1b GmWRKY40 soybean soybean mosaic virus

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H_(2)O_(2)mediates transcriptome reprogramming during soybean mosaic virus-induced callose deposition in soybean

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The Crop Journal 2022年第1期10卷 262-272页

作者： Tianjie Sun Xizhe Sun Fukuan Li Nan Ma Mengxuan Wang Yan Chen Na Liu Yuan Jin Jie Zhang Chunyan Hou Chunyan Yang Dongmei Wang State Key Laboratory of North China Crop Improvement and Regulation Baoding 071000HebeiChina Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology Baoding 071000HebeiChina College of Life Sciences Hebei Agricultural UniversityBaoding 071000HebeiChina Market Supervision Administration of Chongli District Zhangjiakou 076350HebeiChina Institute of Cereal and Oil Crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina

The main defense response to soybean mosaic virus(SMV)infection in soybean[Glycine max(L.)Merr.]is thought to be blockage of intercellular virus transport by callose deposition on plasmodesmata.But the specific regulatory mechanism remains largely unknown.In this study,we found that hydrogen peroxide(H_(2)O_(2))signal downstream of NO was associated with the regulation of callose accumulation.Abundant H_(2)O_(2)was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3,whereas no obvious H_(2)O_(2)was observed in the compatible combination of Jidou 7 and strain SC-8.When H_(2)O_(2)production was inhibited,callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination.The H_(2)O_(2)-associated transcriptome dynamics of soybean during SMV infection was investigated.Transcriptome and functional analysis using virus-induced gene silencing showed that Gm SEOB and Gm PAP27,two genes regulated by H_(2)O_(2),functioned in resistance by positively regulating the accumulation of callose in response to SMV infection.These results lay a foundation for further research on the signal transduction and molecular regulation of callose deposition during soybean resistance to SMV infection.

关键词： Callose soybean mosaic virus H_(2)O_(2) Transcriptome soybean

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Genetic Diversity of Chinese soybean mosaic virus Strains and Their Relationships with Other Plant Potyviruses Based on P3 Gene Sequences

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Journal of Integrative Agriculture 2014年第10期13卷 2184-2195页

作者： YANG Qing-hua LI Kai ZHI Hai-jian GAI Jun-yi soybean Research Institute Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Science and Technology

soybean mosaic virus （SMV）, a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. As the P3 gene is thought to be involved in viral replication, systemic infection, pathogenicity, and overcoming resistance, knowledge of the P3 gene sequences of SMV and other potyviruses would be useful in efforts to know the genetic relationships among them and control the disease. P3 gene sequences were obtained from representative isolates of the above-mentioned 16 SMV strains and were compared with other SMV strains and 16 Potyvirus species from the National Center for Biotechnology GenBank database. The P3 genes from the 16 SMV isolates are composed of 1041 nucleotides, encoding 347 amino acids, and share 90.7-100% nucleotide （NT） sequence identities and 95.1-100% amino acid （AA） sequence identities. The P3 coding regions of the 16 SMV isolates share high identities （92.4-98.9% NT and 96.0-100% AA） with the reported Korean isolates, followed by the USA isolates （88.5-97.9% NT and 91.4-98.6% AA）, and share low identities （80.5-85.2% NT and 82.1-84.7% AA） with the reported HZ 1 and P isolates from Pinellia ternata. The sequence identities of the P3 genes between SMV and the 16 potyviruses varied from 44.4 to 81.9% in the NT sequences and from 21.4 to 85.3% in the AA sequences, respectively. Among them, SMV was closely related to Watermelon mosaic virus （WMV）, with 76.0-81.9% NT and 77.5-85.3% AA identities. In addition, the SMV isolates and potyvirus species were clustered into six distinct groups. All the SMV strains isolated from soybean were clustered in Group I, and the remaining species were clustered in other groups. A multiple sequence alignment analysis of the C-terminal regions indicated that the P3 genes within a species were highly conserved, whereas those among species were relatively variable.

关键词： soybean mosaic virus Potyvirus P3 gene homology analysis phylogenetic tree multiple sequence alignment

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Marker-assisted pyramiding of soybean resistance genes R_(SC4),R_(SC8),and R_(SC14Q) to soybean mosaic virus

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Journal of Integrative Agriculture 2017年第11期16卷 2413-2420页

作者： WANG Da-gang ZHAO Lin LI Kai MA Ying WANG Li-qun YANG Yong-qing YANG Yun-hua ZHI Hai-jian College of Agriculture Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Crop Institute Anhui Academy of Agricultural Sciences/Anhui Key Laboratory of Crops Quality Improving

soybean mosaic virus （SMV） is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, Rsc4, Rsc8, and Rsc14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang l^Kefeng 1 and DabaimaxNannong 1138-2. Ten simple sequence repeat （SSR） markers linked to three resistance loci （Rsc4, Rsc8, and Rsc^4Q） were used to assist pyramided breeding. Pyramided families containing three resistance loci （Rsc4, Rsc8, and Rsc14Q） were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing Rsc4, Rsc8, and Rsc14Q. A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.

关键词： soybean soybean mosaic virus resistance genes pyramiding marker-assisted breeding

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Studies on the Changes of Superoxide Dismutase,Peroxidase and Polyphenoloxidase in Seed Coat of soybeans after Infected with soybean mosaic virus

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Agricultural Sciences in China 2000年第1期 139-142页

作者： Zheng Cuiming soybean Institute Northeast Agricultural UniversityHarbin 150030 Center Laboratory Northeast Agricultural University

Resistant and susceptible soybean varieties were inoculated with soybean mosaic virus(SMV). The seeds were taken for biochemical analysis . The results showed that both peroxidase (POD) and polyphenoloxidase (PPO) activities in seed coat of uninoculated resistant varieties were much higher than those of uninoculated susceptible varieties. Superoxide dismutase (SOD) activity in seed coat of uninoculated resistant varieties was a little stronger than that of susceptible varieties. There were two more bands of PPO and three more bands of POD in uninoculated resistant varieties than those in susceptible varieties. After inoculated with SMV,POD activity was increased a little in susceptible varieties. Both POD and PPO activities were rapidly decreased in seed coat of resistant varieties after inoculated with SMV and one band was vanished. SOD activity was increased in susceptible varieties after inoculated with SMV, while it remained stable in resistant varieties.

关键词： soybean mosaic virus Superoxide dismutase Peroxidase Polyphenoloxidase Seed coat mottling

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Callose deposition during the hypersensitive reaction in soybean infected with soybean mosaic virus

Callose deposition during the hypersensitive reaction in soy...

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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Wenlong LI,Yongshan Zhao,GuibinYao,Sisi Wu,Dongmei Wang College of Life Science,Agricultural University of Hebei,Baoding,071001,China

＜正＞Callose is a β-l,3-glucan.Despite its diverse roles in viral pathogenesis of plants,little is known about the real implication of this molecule in soybean mosaic viruse（SMV）pathogenesis.In this study through ... 详细信息

关键词： Plasmodesmata Callose soybean mosaic virus soybean.

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A codominant SCAR marker linked to SMV resistance gene Rsa

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Progress in Natural Science:Materials International 1999年第7期 41-44页

作者：张志永陈受宜盖钧镒 Beijing 100101 China Chinese Academy of Sciences Institute of Genetics Nanjing 210095 Nanjing Agricultural University soybean Research Institute

The conversion of RAPD (randomly amplified polymorphic DNAs markers to sequence characterized amplified regions (SCAR) counterparts has been proven to be an effective method to obtain more useful and reliable markers. Nine hundred random 10-mer primers were screened using bulked segregant analysis (BSA). Two dominant RAPD markers, OPAS-061800 and OPW-05660, were identified to be linked to Rsa, a single dominant gene resistant to a soybean mosaic virus (SMV) strain Sa. The marker OPW-05660 close to Rsa was cloned and sequenced Two ends of the sequence were used to design a pair of specific oligonucleotide primers. The pair of primers generated polymorphic fragments between resistant parent and susceptible parent, indicating the dominant RAPD marker was successfully converted to a codominant SCAR marker SCW-05 . The genetic distance between SCW-05 and Rsa is 7 .7 cM. The genetic diversity detected by SCW-05 was consistent with resistance or susceptibility in 14 soybean cultivars.

关键词： soybean mosaic virus RAPD sequence characterized amplified regions (SCAR).

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