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A recently evolved BAHD acetyltransferase,responsible for bitter soyasaponin A production,is indispensable for soybean seed germination

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Journal of Integrative Plant Biology 2023年第11期65卷 2490-2504页

作者： Jia Yuan Liya Ma Yan Wang Xindan Xu Rui Zhang Chengyuan Wang Wenxiang Meng Zhixi Tian Yihua Zhou Guodong Wang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed Designthe Chinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100039China State Key Laboratory of Molecular Developmental Biology the Chinese Academy of SciencesBeijing 100190China The Center for Microbes Development and HealthInstitute of Pasteur of Shanghaithe Chinese Academy of SciencesShanghai 200031China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed Designthe Chinese Academy of SciencesBeijing 100101China

soyasaponins are major small molecules that accumulate in soybean(Glycine max)*** them,type-A soyasaponins,fully acetylated at the terminal sugar of their C22 sugar chain,are responsible for the bitter taste of soybean-derived ***,the molecular basis for the acetylation of type-A soyasaponins remains ***,we identify and characterize Gm SSAc T1,encoding a BADH-type soyasaponin acetyltransferase that catalyzes three or four consecutive acetylations on type-A soyasaponins in vitro and in *** analysis and biochemical assays suggest that Gm SSAc T1 likely evolved from acyltransferases present in leguminous plants involved in isoflavonoid ***-of-function mutants of Gm SSAc T1 exhibited impaired seed germination,which attribute to the excessive accumulation of null-acetylated type-A *** conclude that Gm SSAc T1 not only functions as a detoxification gene for high accumulation of type-A soyasaponins in soybean seeds but is also a promising target for breeding new soybean varieties with lower bitter soyasaponin content.

关键词： BAHD acyltransferase seed germination soyasaponin soybean

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Separation of soyasaponins from Alfalfa(Medicago sativa L.)with Macroporous Resin Coupled with Alkaline Ethanol Elution and Their Structure Elucidation

Separation of Soyasaponins from Alfalfa(Medicago sativa L.)w...

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中国植物学会植物结构与生殖生物学专业委员会、江苏省植物学会2007年学术年会

作者： Lang Xu~1 Shu-wen Xu~2 Hong-jing Mao~1 Zhu-nan Gong~(1*) 1 Jiangsu Province Key Laboratory for Molecular and Medical Biology Laboratory,College of Life Science,Nanjing Normal University,Nanjing 210046,China 2 Anhui Analytical Measuring Testing Center,Hefei 230031,China

A mixture of three soyasaponins was separated from alfalfa with macroporous resin coupled with alkaline ethanol elution. The advantage of this method over the natural ethanol elution way is its specificity to the triterpene saponins. The mixture of soyasaponins was obtained by applying:300g of crude extract of alfalfa to 1.2 kg of D101 macroporous resin, the solvent for desorption was water for 10BV,followed by ethanol/water(25:75, v/v)for 10BV and finally KOH/40% ethanol(2:100,w/v)for *** structure of the three soyasaponins was determined using chemical,LC-ESI-MS and NMR *** were identified as soyasaponin Bb,Bc and 3-O-[α-L-rhamnopyranosyl-(1(?)2)-β-D-glucopyranosyl-(1(?)2)-β-D-glucuronopyranosyl]soyasapogenol B. This is the first time that soyasaponin Bc has been identified in the alfalfa plant.

关键词： alfalfa Medicago sativa L soyasaponin LC-ESI-MS

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