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SUMO1 regulates post-infarct cardiac repair based on cellular heterogeneity

Journal of Pharmaceutical Analysis 2023年第2期13卷 170-186页

作者： Zhihao Liu Xiaozhi Liu Li Liu Ying Wang Jie Zheng Lan Li Sheng Li Han Zhang Jingyu Ni Chuanrui Ma Xiumei Gao Xiyun Bian Guanwei Fan First Teaching Hospital of Tianjin University of Traditional Chinese Medicine National Clinical Research Center for Chinese Medicine Acupuncture and MoxibustionTianjin300193China State Key Laboratory of Component-Based Chinese Medicine Tianjin301617China Tianjin Key Laboratory of Epigenetics for Organ Development in Preterm Infants The Fifth Central Hospital of TianjinTianjin300450China Haihe Laboratory of Modern Chinese Medicine Tianjin301617China

Small ubiquitin-related modifier(SUMOylation)is a dynamic post-translational modification that maintains cardiac function and can protect against a hypertrophic response to cardiac pressure ***,the function of SUMOylation after myocardial infarction(MI)and the molecular details of heart cell responses to SUMO1 deficiency have not been *** this study,we demonstrated that SUMO1 protein was inconsistently abundant in different cell types and heart regions after ***,SUMO1 knockout significantly exacerbated systolic dysfunction and infarct size after myocardial ***-nucleus RNA sequencing revealed the differential role of SUMO1 in regulating heart *** cardiomyocytes,SUMO1 deletion increased the Nppa^(+)Nppb^(+)Ankrd1^(+)cardiomyocyte subcluster pro-portion after *** addition,the conversion of fibroblasts to myofibroblasts subclusters was inhibited in SUMO1 knockout ***,SUMO1 loss promoted proliferation of endothelial cell subsets with the ability to reconstitute neovascularization and expressed angiogenesis-related *** analysis of ligand/receptor interactions suggested putative pathways that mediate cardiomyocytes to endothelial cell communication in the *** preinjected with cardiomyocyte-specific AAV-SUMO1,but not the endothelial cell-specific form,and exhibited ameliorated cardiac remodeling following ***,our results identified the role of SUMO1 in cardiomyocytes,fibroblasts,and endothelial cells after *** findings provide new insights into SUMO1 involvement in the patho-genesis of MI and reveal novel therapeutic targets.

关键词： Myocardial infarction SUMO1 snrna-seq Cardiomyocyte

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Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

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Science China(Life Sciences) 2024年第1期67卷 51-66页

作者： Yonghui Jiang Xueying Gao Yue Liu Xueqi Yan Huangcong Shi Rusong Zhao Zi-Jiang Chen Fei Gao Han Zhao Shigang Zhao Center for Reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250012China National Research Center of Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215000China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200127China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of ScienceBeijing 100101China

Obesity,which can arise from genetic or environmental factors,has been shown to cause serious damages to the reproductive *** ovary,as one of the primary regulators of female fertility,is a complex organ comprised of heterogeneous cell types that work together to maintain a normal ovarian microenvironment(OME).Despite its importance,the effect of obesity on the entire ovary remains poorly *** this study,we performed ovary single-cell and nanoscale spatial RNA sequencing to investigate how the OME changed under different kinds of obesity,including high-fat diet(HFD)induced obesity and Leptin ablation induced obesity(OB).Our results demonstrate that OB,but not HFD,dramatically altered the proportion of ovarian granulosa cells,theca-interstitial cells,luteal cells,and endothelial ***,based on the spatial dynamics of follicular development,we defined four subpopulations of granulosa cell and found that obesity drastically disrupted the differentiation of mural granulosa cells from small to large antral ***,HFD enhanced follicle-stimulating hormone(FSH)sensitivity and hormone conversion,while OB caused decreased sensitivity,inadequate steroid hormone conversion,and impaired follicular *** differences can be explained by the differential expression pattern of the transcription factor ***,our study provides a powerful and high-resolution resource for profiling obesity-induced OME and offers insights into the diverse effects of obesity on female reproductive disorders.

关键词： obesity ovarian microenvironment snrna-seq Stereo-seq Foxo1

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A cell transcriptomic profile p ovides insights into adipocytes of porcine mammary gland across development

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Journal of Animal Science and Biotechnology 2024年第1期15卷 182-200页

作者： Yongliang Fan Long Jin Zhiping He Tiantian Wei Tingting Luo Jiaman Zhang Can Liu Changjiu Dai Chao A Yan Liang Xuan Tao Xuebin Lv Yiren Gu Mingzhou Li State Key Laboratory of Swine and Poultry Breeding Industry College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Key Laboratory of Qinghai‑Tibetan Plateau Animal Genetic Resource Reservation and Utilization Southwest Minzu UniversityChengdu 610041China Animal Breeding and Genetics Key Laboratory of Sichuan Province Sichuan Animal Science AcademyChengdu 610000China

Background Studying the composition and developmental mechanisms in mammary gland is crucial for healthy growth of newborns. The mammary gland is inherently heterogeneous, and its physiological function dependents on the gene expression of multiple cell types. Most studies focused on epithelial cells, disregarding the role of neighboring *** Here, we constructed the largest transcriptomic dataset of porcine mammary gland cells thus far. The dataset captured 126,829 high-quality nuclei from physiological mammary glands across five developmental stages(d 90 of gestation, G90;d 0 after lactation, L0;d 20 after lactation, L20;2 d post natural involution, PI2;7 d post natural involution, PI7). Seven cell types were identified, including epithelial cells, adipocytes, endothelial cells, fibroblasts cells, immune cells, myoepithelial cells and precursor cells. Our data indicate that mammary glands at different developmental stages have distinct phenotypic and transcriptional signatures. During late gestation(G90), the differentiation and proliferation of adipocytes were inhibited. Meanwhile, partly epithelial cells were completely differentiated. Pseudo-time analysis showed that epithelial cells undergo three stages to achieve lactation, including cellular differentiation, hormone sensing, and metabolic activation. During lactation(L0 and L20), adipocytes area accounts for less than 0.5% of mammary glands. To maintain their own survival, the adipocyte exhibited a poorly differentiated state and a proliferative capacity. Epithelial cells initiate lactation upon hormonal stimulation. After fulfilling lactation mission, their undergo physiological death under high intensity lactation. Interestingly, the physiological dead cells seem to be actively cleared by immune cells via CCL21-ACKR4 pathway. This biological process may be an important mechanism for maintaining homeostasis of the mammary gland. During natural involution(PI2 and PI7), epithelial cell populatio

关键词： Adipocytes Cell–cell interaction Development Mammary gland snrna-seq

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Single-nucleus RNA sequencing reveals heterogeneity among multiple white adipose tissue depots

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Life Metabolism 2023年第6期2卷 58-73页

作者： Limin Xie Wanyu Hu Haowei Zhang Yujin Ding Qin Zeng Xiyan Liao Dandan Wang Wanqin Xie Hannah Xiaoyan Hui Tuo Deng National Clinical Research Center for Metabolic Diseases and Department of Metabolism and EndocrinologyThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China Key Laboratory of Diabetes Immunology Ministry of Educationand Metabolic Syndrome Research CenterThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China The First Affiliated Hospital Department of OrthopedicsHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangshaHunan 410028China School of Biomedical Sciences The Chinese University of Hong KongHong Kong 999077China Clinical Immunology Center The Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China

Regardless of its anatomical site,adipose tissue shares a common energy-storage role but exhibits distinctive *** the cellular and molecular heterogeneity of white adipose tissue(WAT)is crucial for comprehending its function and ***,existing single-nucleus RNA sequencing(snrna-seq)studies of adipose tissue heterogeneity have examined only one or two *** this study,we employed snrna-seq to test five representative depots including inguinal,epididymal,mesenteric,perirenal,and pericardial adipose tissues in mice under physiological *** analyzing the contents of main cell catego-ries and gene profiles of various depots,we identified their distinctive physiological *** cells and fibro-adipogenic progenitor cells(FAPs)showed dramatic differences among WAT depots,while adipocytes seemed to be *** heightened presence of regulatory macrophages and B cells in pericardial adipose tissues implied their potential contribution to the preservation of coronary vascular ***,the selective aggregation of pericytes within mesenteric adipose tissue was likely associated with the maintenance of intestinal barrier *** a combination of RNA sequencing and snrna-seq analysis,the major subpopulations of FAPs derived from these depots determined the site characteristics of FAPs to a certain *** work estab-lishes a systematic and reliable foundation for investigating the heterogeneity of WAT depots and elucidating the unique roles these depots play in coordinating the function of adjacent organs.

关键词： white adipose tissue adipose tissue heterogeneity adipocyte subpopulations snrna-seq

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