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Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

Chinese Journal of Oceanology and Limnology 2010年第2期28卷 260-266页

作者：鄢庆枇赵敏慧王晓露邹文政陈昌生 Key Laboratory of Science and Technology for Aquaculture and Food Safety Fisheries College of Jimei University

Vibriofluvialis incubated in trypticase soy broth （TSB） showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar （TSA）, and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of K fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that *** adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment.

关键词： Vibrio fluvialis Epinephelus awoara skin mucus adhesion

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Functional C1q is present in the skin mucus of Siberian sturgeon (Acipenser baerii)

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Integrative Zoology 2015年第1期10卷 102-110页

作者： Chunxin FAN Jian WANG Xuguang ZHANG Jiakun SONG Institute for Marine Biosystem and Neuroscience International Center for Marine StudiesShanghai Ocean UniversityShanghaiChina Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of EducationShanghaiChina Institute of Brain Science Fudan UniversityShanghaiChina

The skin mucus of fish acts as the first line of self-protection against pathogens in the aquatic environment and comprises a number of innate immune ***,the presence of the critical classical complement component C1q,which links the innate and adaptive immune systems of mammalians,has not been explored in a primitive actinopterygian *** this study,we report that C1q is present in the skin mucus of the Siberian sturgeon(Acipenser baerii).The skin mucus was able to inhibit the growth of Escherichia *** bacteriostatic activity of the skin mucus was reduced by heating and by pre-incubation with EDTA or mouse anti-human C1q *** also detected C1q protein in skin mucus using the western blot procedure and isolated a cDNA that encodes the Siberian sturgeon C1qC,which had 44.7–51.4%identity with C1qCs in teleosts and tetrapods.A phylogenetic analysis revealed that Siberian sturgeon C1qC lies at the root of the actinopterygian branch and is separate from the tetrapod *** C1qC transcript was expressed in many tissues as well as in *** data indicate that C1q is present in the skin mucus of the Siberian sturgeon to protect against water-borne bacteria,and the C1qC found in the sturgeon may represent the primitive form of teleost and tetrapod C1qCs.

关键词： bacteriostatic activity complement C1qC skin mucus sturgeon

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Antibacterial Activities of Antibacterial Proteins/Peptides Isolated from Organs and mucus of Clarias gariepinus Reared at High Stocking Density

Antibacterial Activities of Antibacterial Proteins/Peptides ...

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2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(CMBB 2010)

作者： Xiaomei Wang Wei Dai Kezhi Xing Tianjun Li and Xin Wang Tianjin Key Laboratory of Aqua-Ecology and Aquaculture Department of Fisheries Science Tianjin Agricultural University Xiqing District Tianjin 300384 China

Antibacterial proteins/peptides are important parts of the innate immune system in Clarias *** examine potential antibacterial proteins/peptides in organs and mucus of ***,crude protein/peptide extracts were isolated with ammonium sulfate precipitation from mucus,skin,gill,suprabranchial organ and *** further extraction using Sephadex G-50 gel filtration chromatography,the proteins/peptides associated with two absorption peaks（AP1 and AP2） were pooled,respectively,and assayed for their antibacterial activities against Escherichia coli,Aeromonas hydrophila and Edwardsiella *** results showed that AP1 and AP2 from all the sampled tissues and mucus at concentration of 100 mg mL-1 exhibited antibacterial activity against the tested bacterial *** in antibacterial activity were observed among sample *** protein profiles of AP1 obtained by Tricine-SDS-PAGE gel showed a broad range of peptides/proteins,and molecular weight of the mutual abundant peptide obtained was about 27 *** activity of AP2 extracted from intestine was due to peptide with molecular weight of 5.5 kDa.

关键词： Antibacterial proteins/peptides Clarias gariepinus epithelial tissues skin mucus antibacterial activities

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