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Anti-inflammatory and analgesic activities with gastroprotective effect of semi-purified fractions and isolation of pure compounds from Mediterranean gorgonian Eunicella singularis

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Asian Pacific Journal of Tropical Medicine 2015年第8期8卷 598-603页

作者： Monia Deghrigue Carmen Festa Lotfi Ghribi Maria Valeria D’Auria Simona De Marino Hichem Ben Jannet Abderrahman Bouraoui Laboratoire de développement chimique galénique et pharmacologique des médicaments (LR12ES09).Equipe de Pharmacologie marineFaculté de pharmacie de MonastirUniversité de Monastir Department of Pharmacy University of Naples "Federico II" Laboratoire de chimie hétérocyclique produits naturels et réactivité. Equipe de chimie médicinale et produits naturels (LR11ES39)Faculté des sciences de MonastirUniversité de Monastir Laboratoire de chimie hétérocyclique produits naturels et réactivité.Equipe de chimie médicinale et produits naturels (LR11ES39)Faculté des sciences de MonastirUniversité de Monastir

Objective: To explore anti-inflammatory activities of organic extract and its semi-purified fractions(ethanol, acetone, methanol/dichloromethane) from the Mediterranean gorgonian Eunicella singularis. Methods: The anti-inflammatory and analgesic activities were evaluated, using the carrageenan-induced rat paw edema model and the acetic acid writhing test in mice. The gastroprotective activity was determined using HCl/Et OH induced gastric ulcers in rats. The purification and structure elucidation of compound(s) from the more effective fraction were determined by chromatographic and spectroscopic methods and in comparison with data reported in the literature. Results: The fraction F-Et OH showed an important antiinflammatory activity associated with significant analgesic and gastroprotective properties. The purification and structure elucidation of compound(s) from this fraction lead to the identification of one diterpenoid and four sterols. Conclusions: These results suggested that components from the active fraction can be used to treat various anti-inflammatory diseases.

关键词： Eunicella singularis Anti-inflammatory activity Analgesic activity Gastroprotective effect Diterpenoid Sterols

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Anticancer activity of diterpenes and steroids from Eunicella singularis against two- and three-dimensional breast cancer cell models

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Journal of Coastal Life Medicine 2017年第12期5卷 531-539页

作者： Sirine Lajili Monia Deghrigue Amal Abdelhamid Snezana Bjelogrlic Christian D.Muller Maria Valeria D’auria Abderrahman Bouraoui Laboratoire de Développement Chimique Galénique et Pharmacologique des Médicaments(LR12ES09).Unitéde Pharmacologie MarineFacultéde PharmacieUniversitéde Monastir5000 MonastirTunisia Institut Pluridisciplinaire Hubert Curien UMR 7178 CNRSFacultéde PharmacieUniversitéde Strasbourg74 Route du Rhin67401 IllkirchFrance National Cancer Research Center of Serbia Pasterova 1411000 BelgradeSerbia Department of Pharmacy University of Naples“Federico II”via D.Montesano 49I-80131 NapoliItaly.

Objective: To investigate the anticancer activity of two diterpenes [palmonine F (C1) and palmonine D (C2)] and three steroids [cholesta-5,22-dien-3β-ol (C3), stigmasterol (C4) and 5α-cholest-5-en-3β-ol (C5)], isolated from the Mediterranean gorgonian Eunicella singularis, against MCF-7 breast cancer cell line. Methods: This study was performed on standard monolayer two-dimensional (2D) model to evaluate apoptosis by means of AnnexinV-FITC/PI flow cytometry and on three-dimensional (3D) spheroid model using Celigo imaging cytometer for spheroids size analysis. Results: Results indicated that both diterpenes and steroids exhibited an important apoptotic activity in a concentration-dependent manner with EC50 values of 13, 49, 30, 66 and 65 μg/mL for C1, C2, C3, C4 and C5, respectively. Treatment of MCF-73D cell model with C1–C5 induced growth regression of spheroids in a concentration-dependent manner similar to the clinical anti-breast cancer drug Taxol;over ten days of incubation, growth rates were singularis, might be used as anti-breast cancer candidate drugs for further development.

关键词： Eunicella singularis Diterpenes Steroids Apoptosis 3D tumor spheroid Flow cytometry

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