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Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-cell-One-Tube

引用

mLife 2022年第4期1卷 448-459页

作者： Zhidian Diao Lingyan Kan Yilong Zhao Jingyun Song Chen Wang Yang Liu Fengli Zhang Teng Xu Rongze Chen Xixian Wang Xiaoyan Jing Jian Xu Yuandong Li Bo Ma CAS Key Laboratory of Biofuels Shandong Key Laboratory of Energy GeneticsSingle-Cell CenterQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina Shandong Energy Institute QingdaoChina Qingdao New Energy Shandong Laboratory QingdaoChina.5Qingdao Single-Cell Biotechnology Co.Ltd.QingdaoChina.

Identification,sorting,and sequencing of individual cells directly from in situ samples have great potential for in-depth analysis of the structure and function of *** this work,based on an artificial intelligence(AI)-assisted object detection model for cell phenotype screening and a cross-interface contact method for single-cell exporting,we developed an automatic and index-based system called EasySort AUTO,where individual microbial cells are sorted and then packaged in a microdroplet and automatically exported in a precisely indexed,“One-cell-One-Tube”*** target cell is automatically identified based on an AI-assisted object detection model and then mobilized via an optical tweezer for ***,a crossinterface contact microfluidic printing method that we developed enables the automated transfer of cells from the chip to the tube,which leads to coupling with subsequent single-cell culture or *** efficiency of the system for single-cell printing is>93%.The throughput of the system for single-cell printing is~120 cells/***,>80%of single cells of both yeast and Escherichia coli are culturable,suggesting the superior preservation of cell viability during ***,AI-assisted object detection supports automated sorting of target cells with high accuracy from mixed yeast samples,which was validated by downstream single-cell proliferation *** automation,index maintenance,and vitality preservation of EasySort AUTO suggest its excellent application potential for single-cell sorting.

关键词： artificial intelligence One-cell-One-Tube(OCOT) single-cell analysis single-cell printing single-cell sorting

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