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Effects of COL1A1 and SYTL2 on inflammatory cell infiltration and poor extracellular matrix remodeling of the vascular wall in thoracic aortic aneurysm

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Chinese Medical Journal 2024年第9期137卷 1105-1114页

作者： Xinsheng Xie Ye Yuan Yulong Huang Xiang Hong Shichai Hong Gang Chen Yihui Chen Yue Lin Weifeng Lu Weiguo Fu Lixin Wang Department of Vascular Surgery Zhongshan Hospital(Xiamen)Fudan UniversityXiamenFujian 361015China Department of Vascular Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Vascular Surgery Institute of Fudan University Fudan UniversityShanghai 200032China

Background:Thoracic aortic aneurysm(TAA)is a fatal cardiovascular disease,the pathogenesis of which has not yet been *** study aimed to identify and validate the diagnostic markers of TAA to provide a strong theoretical basis for developing new methods to prevent and treat this ***:Gene expression profiles of the GSE9106,GSE26155,and GSE155468 datasets were acquired from the Gene Expression Omnibus(GEO)*** expressed genes(DEGs)were identified using the"limma"package in *** absolute shrinkage and selection operator(LASSO),support vector machine-recursive feature elimination(SVM-RFE),random forest,and binary logistic regression analyses were used to screen the diagnostic marker ***-sample gene set enrichment analysis(ssGSEA)was used to estimate immune cell infiltration in ***:A total of 16 DEGs were *** enrichment and functional correlation analyses showed that DEGs were mainly associated with inflammatory response pathways and collagen-related *** type I alpha 1 chain(COL1A1)and synaptotagmin like 2(SYTL2)were identified as diagnostic marker genes with a high diagnostic value for *** expression of COL1A1 and SYTL2 was considerably higher in TAA vascular wall tissues than in the corresponding normal tissues,and there were significant differences in the infiltration of immune cells between TAA and normal vascular wall ***,COL1A1 and SYTL2 expression were associated with the infiltration of immune cells in the vascular wall ***-cell analysis showed that COL1A1 in TAA was mainly derived from fibroblasts and SYTL2 mainly from cluster of differentiation(CD)8+T *** addition,single-cell analysis indicated that fibroblasts and CD8+T cells in TAA were significantly higher than those in normal arterial wall ***:COL1A1 and SYTL2 may serve as diagnostic marker genes for *** upregulation of SYTL2 and COL1A1 may be involved in the inflammat

关键词： Bioinformatics Thoracic aortic aneurysm Immune response single cell analysis Machine learning

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KuiqpickTM:a low cost instrument for tissue microdissection and live single cell collection

KuiqpickTM:a low cost instrument for tissue microdissection ...

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中国神经科学学会第十届全国学术会议

作者： Zhong-Cai Ma Lili C.Kudo Anatol Bragin Stanislav Karsten Neurology David Geffen School of Medicine at UCLA

Objective The current work demonstrates the efficiency of Kuiqpick for brain tissue microdissection with follow-up RNA and protein isolation,dissection of hve brains for neural progenitor cultures,and collection of individual cells from various adherent cell *** Various subanatomical areas of hve,freshly frozen or sucrose-treated mouse brain were dissected,DNA,RNA and proteins were extracted and *** zones were isolated from fresh hve rat brain tissues,and used for neural progenitor cultures(NPC).Individual cells were collected from NPC,SH-SY5Y,CHO and MDA-MB-435 cultures based on morphology or fluorescent *** cells were tested for *** Kuiqpick efficiently collects specific areas of the brain at the cellular *** instrument works in a wide range of parameters and dissects brain sections of various thicknesses ranging from 10 to 500 μ***,fresh frozen and sucrose treated tissues can be used for the dissection of subanatomical regions or even individual *** quality RNA and proteins can be isolated from the collected samples for genomics and proteomics *** employed principle of a gentle vacuum suction has minimal impact on cellular viabihty;therefore,cells collected from cultures or native brain tissues may be used for downstream *** of cells can be performed based on their morphology or fluorescent *** We demonstrated several applications of Kuiqpick in neuroscience research,including(1) brain subanatomical region specific collection for-omics research;(2) collection of brain neurogenic areas for neural progenitor cultures and(3)collection of individual/single cells from in vitro cultures based on their morphology and fluorescent *** low cost of Kuiqpick,its accuracy,ease of use,compatibility with fluorescence,versatility,and compatibility with hve cells and tissues,make it a cell and tissue acquisition system of choice for high qualit

关键词： KuiqpickTM brain microdissection single cell analysis recultivation

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Preparation of Nanopipette for Small Volume Sample analysis

Preparation of Nanopipette for Small Volume Sample Analysis

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2018年中国质谱学术大会（CMSC2018）

作者： Songyue Li Shuting Xu Bai Yu Huwei Liu Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking University

cellular heterogeneity has gradually rose the attention of biochemists, especially in neuron cells. single cell analysis provides significant data averaged in tissue measurement, benefiting the researches on mechanism of brain and neuroscience. However, the limited sample volume and low concentration of analyst leaves it a challenge for the sensitivity of detection method. Sample pretreatment is a widely used method to improve the sensitivity by proper enrichment and dilution process, but in single cell analysis, the small volume makes the sample hard to operate. Here, preparation of nanopipette, nanopipette sampling and induced nano-electrospray ionization is combined, which provides the opportunity for small volume sample/single cell analysis. Modification of the inner wall of capillary was conducted, which aims to improve the selectivity and sensitivity. With the help of nanopipette, the sampling volume of cytoplasm can be controlled, which provides the possibility of quantification. Besides, an unnatural amino acid, 4-Nitro-D-phenylalanine, is used as low-cost internal standard for quantification. Since it doesn’t exist in organism, usage of it may avoid the problem of m/z overlapping.

关键词： single cell analysis Nanopipette Induced nanoESI Modification

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