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Formation of macroporous gel morphology by phase separation in the silica sol-gel system containing nonionic surfactant

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Journal of University of Science and Technology Beijing 2005年第6期12卷 564-571页

作者： Junsheng Wu Xiaogang Li Wei Du Hua Chen Materials Science and Engineering School University of Science and Technology Beijing Beijing 100083 China

The phase separation and gel formation behavior in an alkoxy-derived silica sol-gel system containing C16EO15 has been investigated. Various gel morphologies similar to other sol-gel systems containing organic additives were obtained by changing the preparation conditions. Micrometer-range interconnected porous gels were obtained by freezing transitional structures of phase separation in the sol-gel process. The dependence of the resulting gel morphology on several important reaction parameters such as the starting composition, reaction temperature and acid catalyst concentration was studied in detail. The experimental results indicate that the gel morphology is mainly determined by the time relation between the onset of phase separation and gel formation.

关键词： silica sol-gel phase separation macroporous gel morphology nonionic surfactant

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sol-gel-derived Poly（dimethylsiloxane） Enzymatic Reactor for Microfluidic Peptide Mapping

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Chinese Journal of Chemistry 2006年第7期24卷 903-909页

作者：吴会灵杨芃原范国荣田玉平陆豪杰金红 School of Pharmacy Second Military Medical University the People's Liberation Army Shanghai 200433 China Department of Chemistry Research Center for Proteome Fudan University Shanghai 200433 China

The silica-based poly（dimethylsiloxane）（PDMS） microfluidic enzymatic reactor was reported along with its analytical features in coupling with MALDI TOF and ESI MS. Microfluidic chip was fabricated using PDMS casting and O2-plasma techniques, and used for the preparation of enzymatic reactor. Plasma oxidation for PDMS enabled the channel wall of microfluidics to present a layer of silanol （SiOH） groups. These SiOH groups as anchors onto the microchannel wall were linked covalently with the hydroxy groups of trypsin-encapsulated sol matrix. As a result, the leakage of sol-gel matrix from the microchannel was effectively prevented. On-line protein analysis was performed with the microfluidic enzymatic reactor by attachment of stainless steel tubing electrode and replaceable tip, The success of trypsin encapsulation was investigated by capillary electrophoresis （CE） detection, and MALDI TOF and ESI MS analysis. The lab-made device provided excellent extent of digestion even at the fast flow rate of 7.0 μL/min with very short residence time of ca. 2 s. In addition, the encapsulated trypsin exhibits increased stability even after continuous use. These features are the most requisite for high-throughput protein identification.

关键词： microfluidic enzymatic reactor silica sol-gel matrix-assisted laser desorption ionization time-of-flight mass spectrometry electrospray ionization mass spectroscopy high-throughput protein identification

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