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Evaluation of the stability of shortcut nitrification-denitrification process based on online specific oxygen uptake rate monitoring

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中国化学快报:英文版 2023年第8期34卷 265-269页

作者： Zhouliang Tan Yue Guan Yajun Luo Lin Wang Houzhen Zhou Chong Yang Dan Meng Yangwu Chen CAS Key Laboratory of Environmental and Applied Microbiology Environmental Microbiology Key Laboratory of Sichuan ProvinceChengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China College of Resources and Environmental Engineering Mianyang Normal UniversityMianyang 621000China Department of Materials and Chemical Engineering Yibin UniversityYibin 644000China

shortcut nitrification-denitrification(SCND)is widely concerned because of its low energy consumption and high nitrogen removal ***,the current difficulty lies in the stable maintenance of SCND performance,which leads to the challenge of large-scale application of this new denitrification *** this study,the nitrogen removal pathway from complete nitrification-denitrification(CND)to SCND was rapidly realized under high free ammonia(FA),high pH and low dissolved oxygen(DO)*** variations of specific oxygen uptake rate(SOUR)of activated sludge in both processes were investigated by an online SOUR monitoring *** curves of SOUR from CND to SCND process were observed,and the ammonia peak obtained based on SOUR monitoring could be used to control aeration time accurately in SCND ***,the SOUR ratio of ammonia oxidizing bacteria(AOB)to nitrite oxidizing bacteria(NOB)(SOURAOB/SOURNOB)was increased from 1.40 to 2.93.16S rRNA Miseq high throughput sequencing revealed the dynamics of AOB and NOB,and the ratio of relative abundance(AOB/NOB)was increased from 1.03 to ***,SOURAOB/SOURNOB displayed significant correlations to ammonia removal rate(P<0.05),ammonia oxidation rate/nitrite oxidation rate(P<0.05),nitrite accumulation rate(P<0.05)and the relative abundance of AOB/NOB(P<0.05).Thus,a strategy for evaluation the SCND process stability based on online SOUR monitoring is proposed,which provides a theoretical basis for optimizing the SCND performance.

关键词： shortcut nitrification-denitrification Complete nitrification-denitrification Microbial activity SOUR Process stability

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An innovative membrane bioreactor and packed-bed biofilm reactor combined system for shortcut nitrification-denitrification

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Journal of Environmental Sciences 2009年第5期21卷 568-574页

作者： ZHANG Yunxia, ZHOU Jiti, ZHANG Jinsong, YUAN Shouzhi School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China. School of Environmental and Biological Science and Technology Dalian University of Technology Dalian 116024 China

An innovative shortcut biological nitrogen removal system, consisting of an aerobic submerged membrane bioreactor （MBR） and an anaerobic packed-bed biofilm reactor （PBBR）, was evaluated for treating high strength ammonium-bearing wastewater. The system was seeded with enriched ammonia-oxidizing bacteria （AOB） and operated without sludge purge with a decreased hydraulic retention time （HRT） through three phases. MBR was successful in both maintaining nitrite ratio over 0.95 and nitrification efficiency higher than 98% at HRT of 24 h, and PBBR showed satisfactory denitrification efficiency with very low effluent nitrite and nitrate concentration （both below 3 mg/L）. By examining the nitrification activity of microorganism, it was found that the specifc ammonium oxidization rate （SAOR） increased from 0.17 to 0.51 g N/（g VSS.d） and then decreased to 0.22 g N/（g VSS.d） at the last phase, which resulted from the accumulation of extracellular polymers substances （EPS） and inert matters enwrapping around the zoogloea. In contrast, the average specific nitrite oxidization rate （SNOR） is 0.002 g N/（g VSS.d）, only 1% of SAOR. Because very little Nitrobactor has been detected by fluorescence in situ hybridization （FISH）, it is confirmed that the stability of high nitrite accumulation in MBR is caused by a large amount of AOB.

关键词： shortcut nitrification-denitrification ammonia-oxidizing bacteria nitrite accumulation FISH

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Alternating shortcut nitrification-denitrification for nitrogen removal from soybean wastewater by SBR with real-time control

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Journal of Environmental Sciences 2004年第3期16卷 380-383页

作者： WANGShu-ying GAODa-wen PENGYong-zhen WANGPeng YANGQing DepartmentofEnvironmentalEngineering BeijingPolytechnicUniversityBeijing100022China CollegeofForestResourcesandEnvironment NortheastForestryUniversityHarbin150040China. SchoolofMunicipal＆EnvironmentalEngineering HarbinInstituteofTechnologyHarbin150090China

A novel treating technology for nitrogen removal from soybean wastewater was studied. The process for nitrogen removal was achieved by alternating aeration and mixing, combined with real\|time control strategies. Results showed that the COD and total nitrogen removal rates are more than 90% and 92% at COD and total nitrogen loads of 1\^0-1\^2 kg COD/(kgMLSS·d) and 0\^20-0\^27 kg TN/(kgMLSS·d), respectively. In addition, it could improve sludge settling property. SVI value is less than 70 g/ml during the whole cycles. The method not only may be adapted to treat soybean wastewater with high nitrogen, but also may be applied to treat other high nitrogen wastewater.

关键词： real-time shortcut nitrification-denitrification ORP pH SBR

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Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated Filter integrated system

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Frontiers of Environmental Science & Engineering 2011年第2期5卷 291-297页

作者： Daijun ZHANG Cui BAI Ting TANG Qing YANG Department of Environmental Science Chongqing UniversityChongqing 400030China Key Laboratory of Southwest China Resources Exploitation&Environmental Disaster Control Engineering State Ministry of EducationChongqing UniversityChongqing 400030China

shortcut nitrification-denitrification,anaerobic ammonium oxidation(ANAMMOX),and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter(EGSB-BAF)integrated *** fed different synthetic wastewater with chemical oxygen demand(COD)of 300-1200 mg·L^(-1)and NH_(4)^(+)-N of 30-120 mg·L^(-1)at the outer recycle ratio of 200%,the influence of influent on ANAMMOX in the integrated system was investigated in this *** experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX;however,when an influent with the low concentration of COD was used,the opposite changes could be *** influent NH_(4)^(+)-N concentration favored ANAMMOX when the COD concentration of influent was ***,low COD=NH_(4)^(+)-N ratio would decrease competition for nitrite between ANAMMOX and denitrification,which was favorable for reducing the negative effect of organic COD on *** good performance of the integrated system indicated that the bacterial community of denitrification,ANAMMOX,and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.

关键词： Expanded Granular Sludge Bed-Biological Aerated Filter(EGSB-BAF)integrated system the influence of influent anaerobic ammonium oxidation(ANAMMOX) shortcut nitrification-denitrification methanogenesis

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