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Morphometric and Volumetric Evaluation of Maxillary Sinus as an Aid in sex identification in Forensic Odontology-A Cone-Beam Computed Tomography Study

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Journal of Forensic Science and Medicine 2023年第1期9卷 12-16页

作者： Denny E.Ceena Mohana Bhoraskar Srikant Natarajan Almas Binnal T.S.Bastian Department of Oral Medicine and Radiology Manipal College of Dental SciencesMangaloreKarnatakaIndia Department of Oral Pathology and Microbiology Manipal College of Dental SciencesMangaloreKarnatakaIndia Oral and Maxillofacial Radiology College of DentistryUniversity of FloridaGainsvilleFloridaUS Department of Oral and Maxillofacial Pathology Mahe Institute of Dental Sciences and HospitalMahéKeralaIndia

Background:Various methods are used in forensic odontology for the purpose of sex and personal *** of them include cheiloscopy,rugoscopy,mandibular measurements,and study of paranasal *** our study,we have used maxillary sinus as an aid in sex *** this purpose,we have evaluated the morphometric and volumetric measurements of the maxillary sinus using a 3-Dimensional imaging modality,Cone Beam Computed *** and Objectives:sex and age identification are important in the process of identification of both the living and the ***,this transverse retrospective study was done to appraise the precision of the dimensions and volume of the maxillary sinus to aid in sex and age *** and Methods:Eighty full Field of View(FOV)Cone-Beam Computed Tomography(CBCT)images were retrieved from the archives which were matched with age and *** maxillary sinuses on either side were measured mediolaterally in the axial section and supero-inferiorly in the coronal *** volumetric analysis of the sinus was done in the sagittal *** sex was classified using discriminant function analysis and the differences were compared using independent *** differences with age were analyzed using one-way ***:Independent t-test was done for comparison of the sinuses between both *** sinuses measurements were larger among the males both superior-inferiorly on either side(Psexes on either side(P<0.001 and<0.002 in the case of right and left respectively).On comparing the supero-inferior and mediolateral measurements and the maxillary sinus volume on either side among the various age groups,the values were statistically *** study shows that the maxillary sinus measurements using Cone-beam computed

关键词： Cone‑beam computed tomography forensic odontology maxillary sinus sex identification

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sex identification of Red-crowned Crane by the Polymerase Chain Reaction

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Journal of Northeast Agricultural University(English Edition) 2004年第2期11卷 151-155页

作者： LIJian-hong LIShu-ling BAOJun BAIXiu-juan LifeScienceCollege NortheastAgricuhuralUniversityHarbinHeilongjiang150030PRC LifeScienceCollege NortheastAgricuhuralUniversityHarbinHeilongjiang150030PRC／／CollegeofAnimalScienceNortheastForestryUniversityHarbinHeilongjiang150030PRC

sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.

关键词： Red-crowned Crane sex determining gene sex identification

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Progress in sex identification of Early Embryo of Livestock

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Animal Husbandry and Feed Science 2010年第1期2卷 1-3,10页

作者： ZENG Yu-feng YAN Ping YU Si-jiu GUO Xian LIANG Chun-nian BAO Peng-jia PEI Jie College of Veterinary Medicine Gansu Agricultural University Lanzhou 730070 China Lanzhou Institute of Animal Science and Veterinary Pharmaceutics Chinese Academy of Agricultural Sciences Lanzhou 730050 China

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identification of the sex of Earlier Embryos from Generic Hybrids of Chicken-Quail by Wpkci

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Agricultural Sciences in China 2008年第4期7卷 497-501页

作者： QIAO Ai-jun MA Wen-xia LI Da-quan MENG Qing-mei College of Animal Science and Technology Shihezi University Shihezi 832003 P.R.China

In this study, a protocol was developed to identify the sex of earlier embryos of chicken （♂）-quail （♀） hybrids and successfully tested the sex proportion of each period （66-120 h）. We acquired cross bred eggs by artificial insemination, hatched them in the same batch according to the standard hatching condition of chicken, and collected earlier living embryos at 66, 72, 78, 84, 90, 96, 102, 108, 114, and 120 h randomly. We adopted RT-PCR protocol and multiple PCR, made the known sex quail as the external control, employed fl-actin as the internal control, and used primers that were designed according to conservative area of gene Wpkci of quail to identify the sex of earlier hybrid embryos. The results indicated that the primer of Wpkci can be used to identify the sex of hybrid embryos accurately; there were more male than female in earlier embryos, the sex proportion of earlier embryos compared with academic numerical value was significantly different （P〈0.01）, and there was no difference between different periods （P〉0.05）. In the present study, we concluded that a simple, fast, credible and stable protocol to identify the sex of earlier hybrids embryos had been established by using primer of Wpkci; in earlier embryos, the death rate of female was higher than that of male and there was no fluctuant peak.

关键词： chicken quail hybrids embryos sex identification

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Analysis of the Offspring sex Ratio of Chicken by Using Molecular sexing

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Agricultural Sciences in China 2006年第7期5卷 545-549页

作者： FENG Yan-ping GONG Yan-zhang Nabeel Ahmed Affara PENG Xiu-li YUAN Jin-feng ZHAO Rui-Xia Mohammed Yusuf Osman Jeffer ZHANG Shu-jun College of Animal Science Huazhong Agricultural University Wuhan 430070 P.R.China Department of Pathology Cambridge University Cambridge CB1 2QP UK

The overall sex ratio of offspring （dead embryos and hatch chicks） from all the fertilized eggs of 140 hens collected for 30 days was studied using duplex PCR of certain fragments of sex chromosomes. Additional 894 dead embryos over a period of 21 days of incubation were also investigated to verify the sex ratio of the dead embryos. The sex of the early dead embryos was identified using this molecular sexing technique. The sex ratio of the hatch chicks and the total offspring of the hens investigated in this experiment did not differ from the expected sex ratio （i.e., 1：1）., However, the number of female dead embryos was significantly more than that of males. The data indicated that the different physiologic function of males and females contributed to female-biased mortality during incubation. It was also found by further analysis that the sex ratios of the offspring of some hens were significantly biased to female or male over the period investigated, which suggested that the sex ratio of offspring might be influenced by the maternal condition to some degrees.

关键词： chicken offspring sex ratio sex identification

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Isolation of Fetal Nucleated Red Blood Cells from Maternal Blood

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2000年第2期20卷 169-171页

作者：邹丽叶晓静徐可树朱剑文 Department of Obstetrics & Gynecology Xiehe Hospital Tongji Medical University Wuhan

To find a simple, effective method of isolating fetal cells from maternal peripheral blood for prenatal diagnosis, 45 women were studied with their gestation being 6-14 weeks and age 21- 30 years. The fetal cells were isolated from maternal blood by using discontinuous density gradient centrifugation. Some of the isolated cells were made smear and counted under the microscope; others were used for predicting fetal sex by PCR amplification of Y chromosome specific DYZ1 gene. The major cells in the upper separation interface were lymphocytes and monocytes, with occasionally seen nucleated red blood cells (NRBC); while those in the middle separation interface were neutrocytes, with NRBC scattering. The ratio of NRBC/nucleated cells was 1. 98±0. 28× 10-5. There was no significant difference between the first and second trimester (P>0. 05). The amount of isolated fetal cells was sufficient for prenatal genetic diagnosis. Male pregnancy was correctly predicted in 10 out of 13 cases. It is concluded that the method of discontinuous density gradient centrifugation was of considerable importance in the development of non-invasive prenatal genetic diagnosis.

关键词： maternal peripheral blood nucleated red blood cell sex identification polymerase chain reaction

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