检索结果-南通市图书馆

Association of serotonin transporter gene polymorphisms and major depressive disorder in Chinese Han population

Neural Regeneration Research 2009年第8期4卷 635-640页

作者： Jiyang Pan Ping Ma Liying Huang Jing Tian Huajun Liang Qiaoting Huang Jiwu Liao Hiroshi Kurihara Department of Psychiatry First Affiliated Hospital Jinan University Guangzhou 510630 Guangdong Province China DaAn Gene Co. Ltd. Sun Yat-sen University Guangzhou 510665 Guangdong Province China Institute of Traditional Chinese Medicine and Natural Products Jinan University Guangzhou 510630 Guangdong Province China

BACKGROUND： serotonin transporter （5-HTT） polymorphisms comprise 5-HTT gene-linked polymorphism region （5-HTTLPR） and variable number of tandem repeats （VNTR）. Studies have revealed an association between 5-HTT polymorphism and major depressive disorder, which suggests that the ＂S＂ allele of 5-HTTLPR and Stin2.9 of 5-HTTVNTR are associated with major depressive disorder. However, there are a number of studies that do not support the 5-HTT polymorphism effect in major depressive disorder. OBJECTIVE： To study the relationship between 5-HTT gene polymorphism and major depressive disorder in Chinese Han population. DESIGN, TIME AND SETTING： Case-controlled study of 5-HTT gene polymorphism. The experiment was performed at the Central Laboratory, Third Affiliated Hospital of Sun Yat-sen University, China from March 2005 to January 2006. PARTICIPANTS： A total of 99 depressive patients of Chinese Han nationality were recruited for this study. All patients met DSM-IV diagnostic criteria for major depressive disorder and had a total score of Hamilton Depression Scale （24 items） ≥21 points. In addition, 101 healthy subjects, matched for age and gender, served as the control group. METHODS： Venous blood was collected from all subjects. 5-HTT genotypes and alleles were determined by polymerase chain reaction. Consistent with the Hardy-Weinberg equilibrium, the association between 5-HTT gene polymorphism and major depressive disorder were analyzed by Chi-square test. MAIN OUTCOME MEASURES： 5-HTTLPR and 5-HTTVNTR genotypes and allele frequencies were measured. RESULTS： No significant differences in 5-HTTLPR genotypes and allele frequencies were determined between patients and controls （P 〉 0.05）. However, significant differences in 5-HTTVNTR genotypes and allele frequencies were detected （P 〈 0.01 ）. The Stin2.10 allele and 10/10 genotype associated with major depressive disorder （OR = 2.61,7.7, P 〈 0.05; analysis of dose-response relationships Х^2 = 12.35, P 〈 0.01）. CONCLUSION： Results from the present study revealed no assoc

关键词： serotonin transporter serotonin transporter gene-linked polymorphism region serotonin transporter variable number of tandem repeats polymorphism major depressive disorder

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Meta‑analysis of the Association between serotonin transporter Polymorphisms and Sudden Infant Death Syndrome

引用

Journal of Forensic Science and Medicine 2016年第4期2卷 179-184页

作者： Haojie Qin Guohui Xu Xinmin Pan Yaonan Mo Laboratory of Forensic Pathology School of Forensic MedicineHenan University of Science and TechnologyLuoyang 471003China

The serotonin transporter(5‑HTT)gene has been considered one of the risk factors for sudden infant death syndrome(SIDS),but the association remains unconfirmed.This meta‑analysis was performed to quantitatively summarize the evidence for such a relationship.PubMed,EMBASE,and China National Knowledge Infrastructure databases were searched for eligible studies within a range of published years from 1990 to December 2015.The odds ratios(ORs)with 95%confidence intervals(CIs)were used to assess the different associations.A total of 8 studies with 624 cases and 796 controls were included for 5‑HTT promoter polymorphism,5 studies with 418 cases and 542 controls for intron 2,and 3 studies with 253 cases and 334 controls for haplotype.The pooled examinations showed an overall increased SIDS risk for the 5‑HTT promoter polymorphism(OR=1.65,95%CI=1.03–2.63,P=0.035 for LL vs.LS and SS;OR=1.46,95%CI=1.04–2.04,P=0.028 for L vs.S),but no association(OR=1.00,95%CI=0.75–1.33,P=0.994 for 10+9 carriers vs.12/12;OR=0.97,95%CI=0.79–1.19,P=0.753 for 10+9 vs.12)for intron 2 polymorphism,and an unreliable association(OR=0.52,95%CI=0.31–0.87,P=0.013)for S‑9 and S‑10 haplotypes.This meta‑analysis suggests that the L allele or LL homozygote of 5‑HTT promoter polymorphism has an increased risk for SIDS,while intron 2 polymorphism has no association with SIDS.

关键词： Gene polymorphism meta‑analysis serotonin transporter sudden infant death syndrome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determination of Synthetic Impurities and Metabolic Products of F-ADAM, a Positron Emission Tomography Imaging Agent for serotonin transporter (SERT) Using HPLC-Tandem Mass Spectrometry

引用

Pharmacology & Pharmacy 2020年第10期11卷 282-298页

作者： Wei-Hsi Chen Chyng-Yann Shiue Chemistry Division Institute of Nuclear Energy Research Taiwan PET Center Department of Nuclear Medicine National Taiwan University Hospital Taiwan PET Center Department of Nuclear Medicine Tri-Service General Hospital Taiwan

A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analytical method was developed to determine the identity of impurities resulting from the synthesis of N,N-dimethyl-2-(2-amino-4-fluorophenylthio)benzyl-amine (F-ADAM), as well as its metabolic products by rat liver microsomes. 18F-ADAM is an important positive electron emission ligand commonly employed as a radio-imaging agent for serotonin transporter (SERT) in the brain. F-ADAM and its derivatives were separated using HPLC on a C4-phenyl column with an ammonium formate aqueous buffer/acetonitrile programmed mobile phase. Synthetic contaminants and metabolic products were identified using fragmentation spectra obtained by tandem mass spectrometry. We show that F-ADAM is unstable in methanol, and propose the use of acetonitrile to generate optimal chromatogram. A Cl-substituted species was found to be the major impurity resulting from the F-ADAM synthetic process. The metabolic products of F-ADAM by rat liver microsomes were characterized by oxidization of the sulfur moiety to sulfoxide, demethylation of the dimethylamine moiety, and oxidative defluorination/deamination. These results elucidate the by-products of F-ADAM synthetic and metabolic processes, and provide direction for the application of this imaging agent to biosystems properly.

关键词： Liver Microsomes Metabolites Impurities F-ADAM serotonin transporter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of the 5-HTTLPR and 5-HTTVNTR Polymorphisms in the serotonin transporter Gene in Women with Postpartum Depression

引用

Neuroscience & Medicine 2012年第3期3卷 275-280页

作者： Josi Maria Zimmermann-Peruzatto Silvana Almeida Aldo Bolten Lucion Jean Pierre Oses Luciana Avila Quevedo Karen Amaral Tavares Pinheiro Ricardo Azevedo da Silva Ricardo Tavares Pinheiro Marcia Giovenardi Programa de Pos-Graduacao em Ciencias Biologicas FisiologiaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil Programa de Pos-Graduacao em Ciencias da Saude Universidade Federal de Ciencias da Saúde de Porto AlegrePorto AlegreBrazil Programa de Pós-Graduacao em Saúde&Comportamento Centro de Ciencias da Vida e da SaúdeUniversidade Católica de PelotasPelotasBrazil

Objective: The purpose of the present study was to evaluate the association between the 5-HTTLPR and 5-HTTVNTR polymorphisms in the serotonin transporter gene (SLC6A4) in Brazilian women with diagnosed postpartum depression (PPD) and the presence of depressive symptoms. Method: The cohort consisted of 128 white women who were charac-terized based on skin color and morphological characteristics. The Beck Depression Inventory was used to diagnose PPD and to score the depressive symptoms. The 5-HTTLPR and 5-HTTVNTR polymorphisms were analyzed by PCR-based methods. Results: No association was observed between the PPD diagnosis and either the 5-HTTLPR (p = 0.48) or the 5-HTTVNTR (p = 0.77) polymorphism. When the polymorphisms were analyzed together with haplotype data, the analyses demonstrated that women carriers of the L-12/L-12 diplotype have lower Beck Depression Inventory scores than women carrying other diplotypes (p = 0.04). Discussion: Few studies have investigated the association of SLC6A4 polymorphisms with PPD, and the role of 5-HTTLPR and 5-HTTVNTR polymorphisms in PPD susceptibility has not been established to date. Therefore, our findings link the haplotypes of these two variants with depression symptoms, thereby contributing to our understanding of PPD susceptibility.

关键词： Postpartum Depression serotonin transporter 5-HTTLPR 5-HTTVNTR Polymorphisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Modulation of mifepristone on human brain serotonin transporter SERT activity expressed in Xenopus oocytes

Modulation of mifepristone on human brain serotonin transpor...

引用

中国神经科学学会第十届全国学术会议

作者： Chao-Kun LI Lin-Lin SHAN Xin-Juan LI Lin-Yu WEI Dong-Liang LI Department of Physiology and Neurobiology Xinxiang Medical University

Major depressive disorder is the leading cause of disability worldwide.Glucocorticoids and their receptors are pharmacological target for psychotic depression treatment.Mifepristone,the glucocorticoid receptor antagonist,is an effective candidate for the treatment of psychotic depression and it ameliorates the abnormahties that are related to depressive symptoms.Modulation of serotoninergic neurotransmission is reported as a treatment of psychotic depression.The necessity of serotonin transporter（SERT） in regulation of the psychotic depression is established and changes in SERT activity are involved into the development of psychotic depression.Currently,little is known about the relationship between mifepristone and SERT activity.In the current study,human brain SERT was cloned and expressed on Xenopus oocyte.Two-electrode voltage clamp recording was used to monitor SERT activity.The purpose of the study is to clarify electrophysiological property of mifepristone on the function of human brain SERT.We report here that SERT is a pharmacological target of mifepristone,mifepristone attenuates the activity of SERT by directly closing SERT channel.The amphtude of the serotonin-elicited current is decreased progressively with increasing concentrations of mifepristone and a concentration-dependent outwards current of SERT is induced by mifepristone.The results suggest that mifepristone is an efficient blocker for SERT and the new finding will provide an additional mechanism for evaluating the effect of mifepristone in treatment of psychotic depression.

关键词： Psychotic Depression Mifepristone serotonin transporter Xenopus oocyte

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Increasing cerebrospinal fluid miR-16 levels of depression model rats alleviated depression-like behaviors via influence of the expression of raphe serotonin transporter

Increasing cerebrospinal fluid miR-16 levels of depression m...

引用

2020年浙江省医学会精神病学学术大会浙江省医师协会精...

作者：宋明芬杭州市第七人民医院

Objective:Some evidence indicated that miR-16 may participate in the physiopathology of depression and contribute to the SSRI therapeutic action.The aim of this study was to observe whether increasing cerebrospinal fluid(CSF) miR-16 levels of depression model rats could alleviate their depression-like behaviors and to identify the possible mechanism involved in this process.Methods:Rats in the control group received no treatment,while those in the model,negative control and agomir group were given chronic unpredictable mild stress(CUMS) for 3 weeks.Then the solution of saline,negative control and miR-16 agomir were administrated via intracerebroventricular injection to the model,negative control and agomir group,respectively.The changes of depression-like behaviors,as well as the levels of CSF miR-16,raphe miR-16 and raphe SERT were compared among groups.Results:The injection of agomir increased the sucrose consumption rate and shortened the immobility time as compared with that before injection(both P0.05).Meanwhile,the miR-16 levels in CSF and raphe were obviously higher and the raphe SERT expression was markedly lower in the agomir group than the model group(all P<0.05).Conclusions:Increasing CSF miR-16 elevated the levels of raphe miR-16,thus decreased the SERT expression there,and therefore benefitted depression treatment.

关键词： depression cerebrospinal fluid miR-16 raphe serotonin transporter

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Association Study of serotonin transporter SLC6A4 Gene in Primary Insomnia

Association Study of Serotonin Transporter SLC6A4 Gene in Pr...

引用

中国睡眠研究会第七届西部睡眠医学大会

作者： Wenjuan Chen Yuping Xie Liya Zhou Jing Wang Xubin Wang Xiaoyan Su Peilin Hui Wei Ma Jinfeng Wang Bin Guo Jun Yang Jie Fan Yuan Zhao Shangli Zhang Yu Liu Gansu University of Traditional Chinese Medicine Sleep Medicine Center Gansu Provincial Hospital Electroencephalogram room Gansu Provincial Hospital School of Basic Medical Sciences Lanzhou University

The function of 5-hydroxytryptamine（5-HT or serotonin） is to promote arousal,inhibit slow wave sleep（SWS） and rapid-eye-movement sleep（REMS）.The solute carrier family 6 member 4（SLC6 A4） gene encodes 5-serotonin transporter（SERT）,which uptakes 5-HT from the brain synapses into the presynaptic neuronrapidly.Therefore,it is necessary to explore the association between SLC6 A4 gene polymorphisms and primary insomnia（PI）.A total of 129 patients with PI and 149 healthy controls completed the sleep scales,and underwent polysomnography（PSG）.The association of single-nucleotide polymorphisms（SNPs） in SLC6 A4 gene with PI was observed in all the subjects.All SNPs were genotyped by SequenomMassArray system.There were different degrees of linkage disequilibrium among 4 loci on SERT gene.There was a complete linkage disequilibrium between rs140700 and rs6352（D’=1.000）.A strong linkage disequilibrium was observed between rs2020936 and rs3794808（D’=0.771）,rs3794808 and rs6352（D’=0.993）,rs2020936 and rs140700（D’=0.803）,rs2020936 and rs6352（D’=0.958）,and rs140700 and rs3794808（D’=0.958）.Thehaplotype CGTT showed significant differences between PI group and control group（p=0.027）.All SNPs were distributed in both the groups equally.However,the probability of PI was higher when the genotypes in the four sites were arranged in a combination of CGTT.

关键词： Association study serotonin transporter SLC6A4 gene Primary insomnia Genotypes

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of Ginseng Fruit Saponins on serotonin System in Sprague-Dawley Rats with Myocardial Infarction, Depression, and Myocardial Infarction Complicated with Depression

引用

Chinese Medical Journal 2016年第24期129卷 2913-2919页

作者： Dong-Fang He Yan-Ping Ren Mei-Yan Liu Department of Cardiology Beijing Anzhen Hospital Affiliated to Capital Medical University Beijing 100029 China Geriatric-cardiovascular Department First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi 710061 China

Background： Our previous studies have demonstrated that the levels of 5-hydroxytryptamine （5-HT） and 5-HT 2A receptor （5-HT2AR） in serum and platelet were associated with depression and myocardial infarction （MI）, and pretreatment with ginseng fruit saponins （GFS） before MI and depression had an effect on the 5-HT system. In this study, the effects of GFS on the 5-HT system in the Sprague-Dawley （SD） rats with MI, depression, and MI ＋ depression were evaluated. Methods： A total of eighty SD rats were allocated to four groups： MI, depression, MI ＋ depression, and control groups （n = 20 in each group）. Each group included two subgroups （n = 10 in each subgroup）： Saline treatment subgroup and GFS treatment subgroup. The levels of 5-HT, 5-HT2AR, and serotonin transporter （SERT） were quantified in serum, platelet lysate, and brain tissue through the enzyme-linked immunosorbent assay method, respectively. Results： Compared with those in the saline treatment subgroups, the levels of 5-HT in serum and platelet lysate statistically significantly increased in the GFS treatment subgroups of MI, depression, and MI ＋ depression groups （serum： all P = 0.000; platelet lysate： P = 0.002, 0.000, 0.000, respectively）. However, the 5-HT levels in brain homogenate significantly decreased in the GFS treatment subgroups compared with those in the saline treatment subgroups in MI and depression groups （P = 0.025 and 0.044 respectively）, and no significant difference was observed between saline and GFS treatment subgroups in MI ＋ depression group （P = 0.663）. Compared with that in GFS treatment subgroup of control group, the 5-HT2AR levels in the platelet lysate significantly decreased in GFS treatment subgroups of MI, depression, and MI ＋ depression groups （all P = 0.000）. Compared to those in the saline treatment subgroups, the serum SERT levels significantly decreased in the GFS treatment subgroups in MI, depression, and MI ＋ depression groups （P = 0.009, 0.038, and P = 0.001, respectively）, while the SERT levels of platelet lysate s

关键词： 5-hydroxytryptamine 2A Receptor Depression Ginseng Fruit Saponins 5-hydroxytryptamine Myocardial Infarction serotonin transporter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Association between 5-hydroxytryptamine transporter gene-linked polymorphic region and smoking behavior in Chinese males

引用

Chinese Medical Journal 2009年第12期122卷 1365-1368页

作者： CHU Shui-lian XIAO Dan WANG Chen JING Hang Beijing Chao-yang Hospital Affiliated to Capital Medical University-Beijing Institute of Respiratory Medicine World Health Organization Collaboration Center for Tobacco or Health in China Beijing 100020 China

Background Tobacco use is the major risk factor for numerous health problems. However, only 5% of smokers can successfully quit without therapy owing to the highly addictive properties of nicotine. The serotoninergic system may be involved in smoking behavior because nicotine increases brain serotonin secretion, nicotine withdrawal decreases serotonin levels, and a selective serotonin reuptake inhibitor antagonizes the response to nicotine withdrawal. serotonin transporter （5-HTT） is the most important protein, as it adjusts the serotonin concentration in the synaptic cleft. There is a polymorphism in the upstream regulatory region of the 5-HTT gene, named 5-hydroxytryptamine transporter gene-linked polymorphic region （5-HTTLPR）. Compared with the L allele, the S allele of the polymorphism is associated with decreased transcription efficiency of the 5-HTT gene. In this study, we investigated the relationship between this gene polymorphism and smoking behavior in Chinese males. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed to find 5-HTTLPR gene polymorphisms in 144 smokers and 135 age-matched healthy non-smokers. A questionnaire was completed in all recruited subjects. Results The proportion of L/L （15.3% vs 5.2%） and S/L （50.0% vs 33.3%） genotypes was significantly higher in the smokers than that in the non-smokers （X^2=21.9; P 〈0.01）. The odds ratio （OR） adjusted by age, education, effects of family members and friends who smoke, and alcohol intake was 2.9 （95%CI 1.78-4.80）. In smokers, the number of cigarettes/day （L/L vs S/L vs S/S： 28±12 vs 20±8 vs 16±6, X^2=18.5, P 〈0.01）, smoking index （L/L vs S/L vs S/S： 561±446 vs 393±341 vs 237±201, X^2=12.5, P 〈0.01） and score on the Fagerstrom test for nicotine dependence （FTND）（L/L vs S/L vs S/S： 7.8±1.6 vs 6.2±2.5 vs 3.5±2.1, X2=48.3, P 〈0.01） were significantly higher in smokers with an L/L or S/L genotype than that in the smokers with the S/S genotype. There were no significant differences in the proportion of

关键词： serotonin transporter nicotine dependence 5-hydroxytryptamine transporter gene-linkedpolymorphic region

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

引用

中国药理学与毒理学杂志 2017年第10期31卷 951-951页

作者： Brenda M GANNON Michael H BAUMANN Kenner C RICE Gregory T COLLINS Department of Pharmacology University of Texas Health Science CenterSan AntonioTX 78229 Designer Drug Research Unit Intramural Research ProgramNIDA and NIHBaltimoreMD 21224 Drug Design and Synthesis Section Molecular Targets and Medications Discovery BranchNIDA and NIAAABethesdaMD 20814 South Texas Veterans Health Care System San AntonioTX 78229

OBJECTIVE Wide spread abuse of synthetic cathinones found in bath salts preparations has resulted in regulation of some cathinones internationally.Chemists skirt these laws by altering the chemical structures of first-generation cathinones(ie,MDPV,methylone,and mephedrone),resulting in second-generation cathinones(eg,α-PVP,α-PPP,MDPPP,and MDPBP).Although MDPV is a more effective reinforcer than cocaine,little is known about the reinforcing effectiveness of secondgeneration cathinones.To test the hypothesis that synthetic cathinones with higher selectivity for DAT relative to SERT are more effective reinforcers.METHODS Monoamine transporter inhibition was determined using synaptosomes prepared from rat brains.The relative reinforcing effectiveness of intravenously self-administered MDPV,MDPBP,MDPPP,α-PVP,α-PPP,and cocaine were directly compared through evaluations of (1)dose-response curves under a progressive ratio(PR)schedule of reinforcement and (2)demand curves obtained for each drug in male Sprague-Dawley rats.RESULTS Rank order selectivity for DAT/SERT wasα-PVP>MDPV>α-PPP≈MDPBP>MDPPP>cocaine.Comparisons of the maximum number of infusions obtained under a PR schedule of reinforcement(α-PVP>MDPV>α-PPP>MDPBP≈MDPPP>cocaine)and the essential value obtained for each drug in demand analyses(α-PVP>MDPV>α-PPP≈MDPBP≈MDPPP>cocaine)suggest relative reinforcing effectiveness is related to DAT/SERT selectivity.CONCLUSION These data provide evidence that DAT/SERT selectivity accounts for select synthetic cathinones functioning as more effective reinforcers than cocaine and may predict the abuse-related effects of novel synthetic cathinones in humans.

关键词： synthetic cathinones bath salts self-administration dopamine transporter serotonin transporter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：