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An outlook on coronavirus disease 2019 detection methods

Journal of Pharmaceutical Analysis 2022年第2期12卷 205-214页

作者： Zahra Hassani Nejad Fataneh Fatemi Seyed Ehsan Ranaei Siadat Department of Biochemistry Institute of Biochemistry and BiophysicsUniversity of TehranTehran***Iran Department of Protein Research Protein Research CenterShahid Beheshti UniversityTehran***Iran Sobhan Recombinant Protein Company Research and Development DepartmentTehran***Iran

Diagnostic testing plays a fundamental role in the mitigation and containment of coronavirus disease 2019(COVID-19),as it enables immediate quarantine of those who are infected and contagious and is essential for the epidemiological characterization of the virus and estimating the number of infected cases worldwide.Confirmation of viral infections,such as COVID-19,can be achieved through two general approaches:nucleic acid amplification tests(NAATs)or molecular tests,and serological or antibody-based tests.The genetic material of the pathogen is detected in NAAT,and in serological tests,host antibodies produced in response to the pathogen are identified.Other methods of diagnosing COVID-19 include radiological imaging of the lungs and in vitro detection of viral antigens.This review covers different approaches available to diagnosing COVID-19 by outlining their advantages and shortcomings,as well as appropriate indications for more accurate testing.

关键词： COVID-19 Diagnostics Nucleic acid amplification tests serological tests

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Diagnostic assays for COVID-19:a narrative review

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Journal of Bio-X Research 2020年第4期3卷 123-134页

作者： Liding Zhang Xiaohan Liang Yanqing Li Hua Zheng Wensheng Qu Baofeng Wang Haiming Luo Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics MoE Key Laboratory for Biomedical Photonics Collaborative Innovation Center for Biomedical EngineeringSchool of Engineering Sciences Department of Anesthesiology and Pain Medicine Tongji HospitalTongji Medical College Department of Neurology Tongji HospitalTongji Medical College Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Coronavirus disease 2019(COVID-19)is a newly emerged infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The number of COVID-19 cases is continuously increasing and no effective drugs or vaccines are currently available.Accurate and efficient diagnostic testing methods are desperately needed for the detection of SARS-CoV-2 and antiviral antibodies in infected individuals.Various assay techniques,including nucleic acid tests[eg,polymerase chain reaction(PCR),reverse transcription-PCR,real-time loop-mediated isothermal amplification,and CRISPR-Cas-based detection],serological tests[eg,immunoglobulin(Ig)A,IgM/G],imaging tests(eg,computed tomography and positron-emission tomography),and nanoparticle-based detections have been reported for COVID-19 diagnosis.This review aims to present the current diagnostic tools for SARS-CoV-2 and their performance characteristics to inform the appropriate selection of diagnostic and surveillance technologies at optimal testing times.We also describe the advantages of detection using combined nucleic acid and imaging tests,or serological testing and point-of-care diagnostics.Developing reliable protein biomarkers targeting the conserved proteins of SARS-CoV-2 rather than IgA,IgM,or IgG would be useful to manage SARS-CoV-2.

关键词： COVID-19 imaging tests nucleic acid tests point-of-care diagnostics serological tests

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