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Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G_1 phase

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 336-342页

作者： Cheol Park Ji-Suk Jeong Jin-Woo Jeong Sung Ok Kim Yong-Joo Kim Gi-Young Kim Su-Hyun Hong Yung Hyun Choi Department of Molecular Biology College of Natural Sciences and Human EcologyDongeui University Department of Research and Development of Resources Gurye-gun Agricultural Center Wild Flower Institute.Gurye-gun Anti-Aging Research Center.Dongeui University Department of Food Science & Biotechnology College of EngineeringKyungsung University Republic of Korea Laboratory of Immunobiology.Department of Marine Life.Sciences.Jeju National University Department of Biochemistry.Dongeui University

Objective:To investigate the effects of an ethanol extract of Kalopanax septemlobus(Thunb.)***(EEKS) on cell proliferation in human hepatocellular carcinoma cells and its mechanisms of ***:Cells were treated with EEKS and subsequently analyzed for cell proliferation and flow cytometry *** of cell cycle regulators were determined by reverse transcriptase polymerase chain reaction analysis and Western blotting,and activation of eyclin-associaled kinases studied using kinase ***:The EEKS suppressed cell proliferation in both HepG2 and Hep3 B cells,but showed a more sensitive anli-proliferative activity in HepG2 *** cytometry analysis revealed an association between the growth inhibitory effect of EEKS and with G_1 phase cell cycle arrest in HepG2 cells,along with the dephosphorylation of retinoblastoma protein(pRB) and enhanced binding of pRB with the E2 F transcription factor family *** with EEKS also increased the expression of cyclin-dependent kinase(CDK) inhibitors,such as p21WAF1/CIP1 and *** any noticeable changes in G_1 cyclins and CDKs(except for a slight decrease in CDK4).Treatment of HepG2 cells with EEKS also increased the binding of p21 and p27 with CDK4 and *** was paralleled by a marked decrease in the cyclin D- and cyclin E-associated kinase ***:Overall,our findings suggest that EEKS may be an effective treatment for liver cancer through suppression of cancer cell proliferation via G_1,cell cycle arrest Further studies arc required to identify the active compounds in EEKS.

关键词： Kalopanax septemlobus Hepatocellular carcinoma G1 cell cycle arrest CDK inhibitor pRB

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Seedling Culture of Kalopanax septemlobus

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Journal of Forestry Research 1998年第1期9卷 34-37页

作者：刘财富郑景明 Liaoning Academy of Forestry Science Shenyang P. R. China

Experiments on temperature-accelerated germination. seeding quantity, and optimum density of seedlings were made for Kalopanax septemlobus. The experimental results indicated that the seeds of kalopananx septemlobus had physiological dormancy because of unmature embryo and existence of inhibitory matter in the seed. Temperature-accelerated germination was a preferable and essential way to assure the germination. Seed dormancy could be overcome through accelerating germination. Firstly, warm treatment should be done, morphological maturity occurred, then, followed by lower temperature treatment, reaching physiological maturity, Seeding quantity, optimum density and the relations between density of seedlings and yield were studied.

关键词： Kalopanax septemlobus Seed germination Temperature Seed dormancy Optimum density Seedlings

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