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separation and purification of GST-glycerol-3-phosphate Dehydrogenase

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Agricultural Biotechnology 2016年第5期5卷 44-45页

作者： Hongmei ZHAO Shihai LI Yasuo WATANABE College of Animal Sciences Yangtze University Agriculture Department Ehime University

In order to investigate the expression of glycerol-3 -phosphate dehydrogenase by GCY1 gene in recombinant Saccharomyces cerevisiae, induction culture of the S. cerevisiaestrain was performed with SD-URA 2% galactose, 3 × YP ＋ 6% glucose, SC-URA 2% galactose, and SC-URA 2% galactose ＋ 5% NaCI glyeerol-3-phosphate dehydregenase, the cultured S. cerevisiaewas comminuted followed by full-automatic high-speed purification, and SDS-PAGE gel electrophoresis was performed for molecular weight of the GST fusion protein. The results showed that after shaking culture of the S. cerevisiae containing GCY1 at 25 ℃, the OD values of its 3 × YP ＋ 6% glucose culture and SC-URA 2% galaetose ＋ 5% NaC1 culture were 8.75 and 7.35, respectively. It was shown by purification with a Profinia low-pressure liquid chromatograph that only the S. cerevisiae cultured in SC-URA 2% galactose ＋ 5% NaC1 medium expressed glycerel-3-phosphate de- hydrogenase, the molecular weight of which was detected as 65 ku by SDS-PAGE gel electrophoresis.

关键词： Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase Galactose SDS-PAGE gel electrophoresis separation and purification

separation and purification of Recombinant Hirudin Variant 3 from Bacillus subtilis

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Agricultural Science & Technology 2009年第6期10卷 15-19页

作者：陈华友齐向辉耿旭徐庆刚江苏大学江苏镇江212013 南京工业大学江苏南京210009 河南大学医学院河南开封475004

[ Objective] The research aimed to get the optimized separation and purification conditions of the hirudin produced from Bacillus subtilis DB403 （pUBH5）. [Method] Through the systemic pretreatment, preliminary chromatography and fine chromatography. [Result]The optimized separation and purification conditions were that： Supernatant was treated by trichloroacetic acid, then by ultrafiltration desalt and anion exchange chromatography. Strong anion Q F. F. was better than weak anion DEAE F.F. The proper balanced solution was Tris-HCI （ pH 8.0）. The proper conductivity was 6 ms/cm. The maximum applied sample was 240 ATU/ml to matrix of strong anion Q F. F. This optimized procedure was magnified in strong anion exchange HiPrep 16/10Q with the 90% recovery and 70.2% purity. The purification of gel filtration of Sephacryl S-100 to hirudin was not relative to flow rate within certain scope. The application size of sample was 10 ml. The purity checked by HPLC was 95.1%, and the recovery was 93%, and the band of SDS-PAGE was single. [ Conclusion] The research provided the reference of the further industrialization separation and purification of hiruin.

关键词： Hirudin Bacillus subtilis Ion exchange separation and purification

Development of Rare Earth Hydrometallurgy Technology in China

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Journal of Rare Earths 2005年第1期23卷 1-4页

作者：黄小卫龙志奇李红卫应娓娟张国成薛向欣 General Research Institute for Non-Ferrous Metals Grirem Advanced Materials Co. Ltd. Beijing 100088 China Institute of Metallurgical Resources & Environmental Engineering Northeastern University Shenyang 110004 China

In this paper, the recent advance of processes for treating Baotou mixed-type rare earths concentrate, bastnasite, and ionic adsorption deposit in China was reviewed. The R & D circumstance and new progresses in separation and purification of individual and high purity RE compounds in China were introduced. Moreover, the development of RE hydrometallurgy was also predicted. So we suggest to enhance the research and development of products applying RE, especially Ce, Y, Gd, Sm etc., for keeping RE consumption equilibrium and extending applications, to increase enterprise's automatic control level for improving the stability and the consistency of the products, and to pay more attention to protection of environment.

关键词： rare earths hydrometallurgy separation and purification

Technology development for rare earth cleaner hydrometallurgy in China

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Rare Metals 2015年第4期34卷 215-222页

作者： Xiao-Wei Huang Zhi-Qi Long Liang-Shi Wang Zong-Yu Feng National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous Metals Grirem Advanced Materials Co. Ltd.

China is the global leader in rare earth （RE） production using hydrometallurgical processes. Advantageous extraction techniques of rare earths from Baotou mixed rare earth minerals, bastnaesite, and ion-adsorption clays of rare earth deposits have been developed in China. The separation and purification technologies have also achieved rapid development. The industrial application processes for rare earth hydrometallurgy were summarized in the present paper. With the large demands and rapid development of rare earths, the issues of resources and environment are more prominent. This review gives an overview of the main processes that were developed in the past toward greener hydrometallurgy of rare earths in China. Based on the development of the rare earth industry, comprehensive utilization and cleaner production should still be focused on in the future, to support the sustainable development.

关键词： Rare earths Cleaner production separation and purification Hydrometallurgy

purification and characterization of Al_(13) species in coagulant polyaluminum chloride

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Journal of Environmental Sciences 2009年第1期21卷 18-22页

作者： GAO Baoyu CHU Yongbao YUE Qinyan WANG Yan School of Environmental Science and Engineering Shondong University Jinan 250100 China. School of Environment and Safety Engineering Qingdong University of Science and Technology Qingdao 266042 China Key Laboratory of Colloid and Interface Chemistry Minstry of Education Shandong University Jinan 250100 China

Nano-Al13 was separated and purified by four methods to investigate its characteristic, and was analyzed by Al-Ferron timed complexation spectrophotometer, ^27Al-NMR （nuclear magnetic resonance）, and transmission electron microscopy （TEM）. Coagulation efficiency of nano-Al13, polyaluminum chloride （PAC）, and AlCl3 in synthetic water were also investigated by jar test. The dynamic process and aggregation state of kaolin suspensions coagulating with nano-Al13, PAC, and AlCl3 were also investigated. The experimental results indicated that the efficiency of gel column chromatography method was the highest for separating PAC solution with low Al concentration. Ethanol and acetone method was simple and could separated PAC solution with different Al concentrations, while silicon alkylation white block column chromatography method could separate PAC solution only with low Al concentration. The SO4^2-/Ba^2＋ displacement method could separate PAC solution with high Al concentration, but extra inorganic cation and anion could be introduced into the solution during the separation. The coagulation efficiency and dynamic experimental results showed that nano- Al13 with a high positive-charged species was the main species of electric neutralization in coagulation process, and it could reduce the turbidity and increase the effective particles collision rate efficiently in coagulation process. Its coagulation speed and the particle size of coagulant formed were of greatest value in this study.

关键词： nano-Al13 separation and purification coagulation dynamic processes

Recovery of platinum from spent automotive catalyst based on hydrometallurgy

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Rare Metals 2023年第4期42卷 1118-1137页

作者： Tao Ge Jin-Dong He Liang Xu Yan-Hang Xiong Ling Wang Xiao-Wei Zhou Yong-Pan Tian Zhuo Zhao School of Metallurgical Engineering Anhui University of TechnologyMa’anshan243032China State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Kunming650093China Low-Carbon Research Institute Anhui University of TechnologyMa’anshan243032China

Platinum(Pt)is a critical raw material for automotive catalytic converters due to its high-temperature stability,corrosion resistance and catalytic activity,whereas its limited primary resources and uneven distribution make it hard to meet the growing demand of *** automotive catalyst(SAC)is currently the most important secondary resource of platinum,of which the platinum content is much higher than that of the primary platinum *** recovery process of platinum from spent automobile catalyst mainly consists of pretreatment followed by enrichment and refining,involving pyro-and hydrometallurgical techniques,among which enrichment and refining processes are extremely important for platinum recovery from spent automobile *** paper provides an overview of the technologies for platinum recovery from spent automotive *** emphasis is placed on the processes of enrichment and refining based on hydrometallurgical *** directions of research and development of platinum recovery from spent automobile catalyst are also proposed.

关键词： Platinum(Pt) Platinum group metals Spent automotive catalyst(SAC) Hydrometallurgy Solvent extraction separation and purification

A new microfiltration membrane with three-dimensional reticular architecture for Nano-pollutants removal from wastewater

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Progress in Natural Science:Materials International 2021年第3期31卷 414-419页

作者： Wanyuan Gui Jingru Liu Xiping Song Hui Zhang Junpin Lin Benli Luan National Center for Materials Service Safety State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Department of Chemistry Western University Qian Xuesen Laboratory of Space Technology China Academy of Space Technology

This research provides a new insight into designing next-generation filtration materials for Nano-pollutants removal from wastewater, and potentially for gas/solid or liquid/solid separation in corrosive *** the high corrosion and oxidation resistance, Ti–40 Al–10 Nb–10 Cr porous alloy was prepared by powder metallurgy and used as a mechanical support with a pore size of around 10 μm. Subsequently, metallic Zn nanoparticles was coated on the Ti–40 Al–10 Nb–10 Cr porous framework by means of chemical vapor deposition,obtaining a novel microfiltration membrane with three-dimensional reticular architecture. Furthermore, the influence of Zn coating on surface morphology, pore feature, and filtration capability of Ti–40 Al–10 Nb–10 Cr porous alloy was investigated. It is of note that the as-prepared microfiltration membrane exhibits an excellent Nanopollutants filtration efficiency of greater than 99.989% from wastewater. Most importantly, such a threedimensional reticular architecture provides excellent structural stability, allowing the reutilization of the microfiltration membrane for at least 200 filtration cycles of wastewater treatment.

关键词： Porous materials Microfiltration membrane separation and purification Intermetallic materials Water purification

The Herbicidal Activity of Mutant Isolates from Botrytis cinerea

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Agricultural Sciences in China 2006年第8期5卷 622-628页

作者： ZHANG Jin-lin ZHANG Li-hui LIU Ying-chao MA Juan LI Chuan DONG Jin-gao Mycotoxin Laboratory Hebei Agricultural University Baoding 071001 P.R.China

Fifteen mutant isolates were obtained by ultraviolet mutation from parent isolate Botrytis cinerea BC-4. Among them three mutant isolates, BC4-1, BC4-2, and BC4-15, showed strong herbicidal activity. BC4-1 showed maximum herbicidal activity for inhibition of germination and growth of Digitaria sanguinalis L. and Arnaranthus retroflexus L. The results also showed that herbicidal activity was influenced by differing pH of PD media, with pH value of 4.0 being the optimum. The crude toxin was extracted using chloroform, petroleum ether, and ethyl acetate, respectively, and the ethyl acetate extracts showed the strongest inhibitory activity on the germination and growth of D. sanguinalis L. and A. retroflexus L. Using HPLC, one fraction with an absorption peak at 271 nm was separated from the crude toxin. This fraction could strongly inhibit the growth of D. sanguinalis L. at a concentration of 100 mg L^-1 and could completely inhibit the seed germination of D. sanguinalis L. and A. retroflexus L. at a concentration of 50 mg L^-1 .

关键词： Botrytis cinerea toxin ultraviolet mutation herbicide activity separation and purification

Separating method and dynamic processes of Nano-Al_(13)

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Frontiers of Environmental Science & Engineering 2007年第3期1卷 368-373页

作者： GAO Baoyu CHU Yongbao YUE Qinyan KONG Chunyan WANG Xiaona School of Environmental Science and Engineering Shandong UniversityJinan 250100China College of Material and Environment Qingdao University of Science and TechnologyQingdao 266042China Department of Chemistry Dezhou UniversityDezhou 253023China

In order to investigate the characteristics of pure Nano-Al_(13) ,Nano-Al_(13) was separated and purified from a series of poly-aluminum chloride(PAC)solutions which had the same Al_(13) percentage but different total Al concentrations,by using column chromatography,ethanol-acetone resolving and SO_(4)^(2−)/Ba^(2+)*** Al_(13) species yield was characterized by Al-ferron timed complexation spectropho-tometry and 27Al-NMR(nuclear magnetic resonance).The coagulation efficiency of Nano-Al_(13) ,PAC and AlCl_(3) in synthetic water was also investigated by Jar *** dynamic process and aggregation state of kaolin suspensions coagulating with Nano-Al_(13) ,PAC and AlC_(13) were similarly investigated using a photometric dispersion analyzer 2000(PDA2000).The experimental results indicated that the ethanol-acetone resolving method was simple and could separate the PAC solution at different concentrations,while column chromatography could separate PAC solutions at low *** SO_(4)^(2−)/Ba^(2+)displacement method could separate PAC solutions at high ***,extra inorganic cation and anion could be added in the solution during *** coagulation efficiency and dynamic experimental results showed that Nano-Al_(13) with high positive-charged species was effective in removing turbidity and *** dynamic process results showed that Nano-Al_(13) also had the best recovery capability after shearing compared with PAC and AlCl3 because the Nano-Al_(13) conformation is more effective in charge neutralization.

关键词： Nano-Al_(13) separation and purification coagula-tion dynamic processes