检索结果-南通市图书馆

Pseudolaric acid B induces apoptosis,senescence,and mitotic arrest in human breast cancer MCF-7

Acta Pharmacologica Sinica 2007年第12期28卷 1975-1983页

作者： Shin-ichi TASHIRO Satoshi ONODERA Takashi IKEJIMA China-Japan Research Institute of Medical Pharmaceutical Sciences Shenyang Pharmaceutical UniversityShenyang 110016China Department of Clinical and Biomedical Sciences Showa Pharmaceutical University Tokyo 194-8543Japan

Aim:The aim of the present study was to investigate the inhibitory effect ofpseudolaric acid B (PAB) on human breast cancer MCF-7 cells.Methods:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis,morphologicalchanges,acridine orange staining,and agarose gel electrophoresis were appliedto detect apoptosis.The percentage of apoptotic and necrotic cells was calcu-lated by the lactate dehydrogenase activity-based cytotoxicity assay;senescenceassociated (SA)-β-galactosidase activity was detected to evaluate senescence;flow cytometric analysis of propidium iodide staining was carried out to investi-gate the distribution of cell cycle,and the protein expression was examined byWestern blot analysis.Results:During apoptosis,the half maximal inhibitoryconcentration IC50was 3.4 and 1.35 μmol/L at 36 and 48 h after PAB treatment,respectively.The MCF-7 cells exposed to PAB showed typical characteristics ofapoptosis,including the morphological changes and DNA fragmentation.TheMCF-7 cells treated with 4 μmol/L PAB for 36 h underwent apoptosis,but notnecrosis.The apoptosis induced by PAB was independent of the death receptorpathway.The senescent cells became larger and flatter,and the SA-β-galactosi-dase staining was positive.PAB induced obvious mitotic arrest and it precededapoptosis and senescence.The expressions of p21 and p53 was upregulated withPAB treatment,and cyclin B 1 was upregulated and transported from the cyto-plasm to nuclei,and sustained stable levels.Conclusion:PAB induced mitoticarrest in the MCF-7 cells and inhibited proliferation through apoptosis andsenescence.The apoptosis was independent of the death receptor pathway.

关键词： apoptosis senescence mitotic arrest human breast cancer MCF-7 pseudolaric acid B

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant senescence

引用

Agricultural Sciences in China 2011年第4期10卷 534-543页

作者： LIN Ling-ling SHI Qing-hua WANG Hua-sen QIN Ai-guo YU Xian-chang Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 t~R. China College of Horticultural Science and Engineering Shandong Agricultural University~State Key Laboratory of Crop Biology Tai ＇an271018 P.R. China College of Agriculture and Food Science Zhejiang Forestry University Lin ＇an 311300 P.R. China

GDP-D-mannose pyrophosphorylase （GMPase） catalyses the synthesis of GDP-D-mannose and represents the first committed step in the synthesis of ascorbate. In the present study, the GMPase gene of tomato was introduced into potato by Agrobacterium-mediated transformation. Two transgenic lines with higher GMPase expression were selected using qPCR and protein blot analyses. The results showed that the content of L-ascorbic acid （AsA） and the ratio of AsA/ DHA （dehydroascorbate） significantly increased in both leaves and tubers of transgenic potato plants. Both pigment content and photosynthetic rate were much higher in transgenic plants than in wild-type plants. Transgenic plants showed a distinguishable change in phenotype from the wild-type plants. Furthermore, transgenic plants showed delayed senescence.

关键词： potato GDP-mannose pyrophosphorylase ascorbate senescence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of Organic Substances Associated with Tissue senescence in Upland Cotton (Gossypium spp. L.) Based on GC-MS Analysis

引用

Agricultural Sciences in China 2011年第8期10卷 1197-1205页

作者： XU Zhen-long GUO Cheng-jin GU Jun-tao LU Wen-jing LI Xiao-juan XIAO Kai College of Agronomy Agricultural University of HebeiBaoding 071001P.R.China College of Life Science Agricultural University of HebeiBaoding 071001P.R.China

Premature senescence in crop production,especially occurred at the late growth stage,generally results in a reduction in yield and quality.Therefore,it is beneficial for yield and quality to properly delay senescence of plant tissues during the late developmental stage.In this study,it was observed that the chlorophyll content and photosynthetic rate were gradually decreased along leaf growth progression,and the rates of reduction were promoted by drought.Based on gas chromatography-mass spectrometry （GC-MS） analysis,total eight,five,seven,and five kinds of organic compounds that putatively associated with the tissue senescent progression were identified in leaves,fruit branches,petals,and sepals,respectively.It was found that the identified organic compound,such as a-pinene,b-pinene,and pentadecane were present in different tissues.Among the total ten organic substances identified to be related with the leaf senescence,half were specifically detected in the drought treatment.These results suggest some biochemical pathways associated with the leaf senescence are distinctly regulated by drought.The identified organic compounds in the tested tissues showed three types on the performance pattern based on the contents along with the senescent progression,including gradually increasing,decreasing,and a curve with one single peak.Thus,during the senescence process in tissues,a subset of metabolic substances occur modifications on the quantities,reflecting a complicate biochemical reactions are initiated via the senescence signals.Further analysis of the important organic substances will be helpful for elucidation of the tissue senescence mechanism at the biochemical level and provide a new insight of the senescence signaling transductions in cotton.

关键词： cotton （Gossypium spp. L.） senescence organic substance identification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ganoderma atrum polysaccharide relieves mitochondrial dysfunction to alleviate hydrogen peroxide-induced senescence via activating autophagy

Journal of Future Foods

引用

Journal of Future Foods 2022年第3期2卷 241-252页

作者： Mi Guo Ke Zhang Duoduo Zhang Yujia Zhou Linyuan Liu Yu Wu Xingtao Zhou Shaoping Nie State Key Laboratory of Food Science and Technology China-Canada Joint Laboratory of Food Science and Technology(Nanchang)Key Laboratory of Bioactive Polysaccharides of Jiangxi ProvinceNanchang UniversityNanchang 330047China

Polysaccharides from genus Ganoderma have prominent anti-aging effect,but their mechanisms are incompletely clarified.In our previous experiments,Ganoderma atrum polysaccharide(PSG)was exhibited to significantly alleviate senescence and DNA damage of A375 cells.To investigate its underlying mechanism,we conducted RNA-Seq analysis and found that PSG upregulated autophagy and mitochondria pathways compared to the model group.Further experiments showed that PSG relieved mitochondrial dysfunction via inhibiting the accumulation of reactive oxygen species(ROS)and loss of the mitochondrial membrane potential(MMP).Meanwhile,PSG activated autophagy and enhanced lysosomal function through transcription factor EB(TFEB).Interestingly,the pretreatment of autophagy inhibitors bafilomycin A1(Baf A1)reversed the ROS decline induced by PSG,which subsequently increased cellular senescence of A375.In addition,PSG had a similar anti-aging effect on normal fibroblast WI-38.Taken together,PSG might reduce ROS levels through activating autophagy,which in turn suppressed mitochondrial dysfunction and cellular senescence.

关键词： Ganoderma atrum polysaccharide senescence Reactive oxygen species Autophagy Mitochondrial dysfunction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2

引用

Horticulture Research 2021年第1期8卷 2107-2120页

作者： Kuo Yang Jian-Ping An Chong-Yang Li Xue-Na Shen Ya-Jing Liu Da-Ru Wang Xing-Long Ji Yu-Jin Hao Chun-Xiang You National Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China National Key Laboratory of Crop Biology College of Life ScienceShandong Agricultural UniversityTai-AnShandong 271018China

Jasmonic acid(JA)plays an important role in regulating leaf senescence.However,the molecular mechanisms of leaf senescence in apple(Malus domestica)remain elusive.In this study,we found that MdZAT10,a C2H2-type zinc finger transcription factor(TF)in apple,markedly accelerates leaf senescence and increases the expression of senescence-related genes.To explore how MdZAT10 promotes leaf senescence,we carried out liquid chromatography/mass spectrometry screening.We found that MdABI5 physically interacts with MdZAT10.MdABI5,an important positive regulator of leaf senescence,significantly accelerated leaf senescence in apple.MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1,thus accelerating leaf senescence.In addition,we found that MdZAT10 expression was induced by methyl jasmonate(MeJA),which accelerated JA-induced leaf senescence.We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation,thereby delaying MdZAT10-mediated leaf senescence.Taken together,our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.

关键词： domestica C2H2 senescence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Liuwei Dihuang decoction and its compatible prescriptions on the function of hypothalamus-pituitary-adrenal axis in senescence-accelerated mouse

引用

中国药理学与毒理学杂志 2015年第S1期29卷 74-75页

作者： Ning JIANG Si-di LI Wen-xia ZHOU Yong-xiang ZHANG Beijing Institute of Pharmacology and Toxicology China Beijing 100850

OBJECTIVE To investigate age-related functional change of hypothalamus-pituitary-adrenal(HPA)axis in senescence accelerated mouse(SAM)and the effects of Liuwei Dihuang decoction(LW)and its San-bu(three tonics)and San-xie(three eliminators)components on the function of HPA axis.METHODS Male SAM-resistance/1(SAMR1)and SAM-prone/8(SAMP8)at the ages of 6and 12 months old were used.SAMP8 were orally administered with LW,three tonics and three eliminators at the doses of 10,6.4and 3.6g·kg-1·d-1,respectively,for consecutive 60 d.Serum level of CORT was assayed with ELISA method.The levels of hypothalamic CRH and pituitary ACTH was determined with radioimmunoassay.RESULTS The levels of hypothalamic CRH,pituitary ACTH and serous CORT were much higher in 6 and 12 months old SAMP8 than those in age-matched SAMR1,which suggested the abnormal function of HPA axis in SAMP8.Oral administration of LW and three tonics significantly decreased the level of hypothalamic CRH of 6 and 12 months old SAMP8,and reduced the levels of pituitary ACTH and serous CORT of 6 month old SAMP8.Three eliminators significantly decreased the level of hypothalamic CRH of 6 months old SAMP8.The results indicated that oral administration of LW,three tonics and three eliminators improved the function of HPA axis of SAMP8.CONCLUSION The results showed the hyperactivity of HPA axis of SAMP8,and LW improved the hyperactivity of 6 month old SAMP8.Three tonics and three eliminators had similar effects as LW,which had better effect after compatibility.

关键词： Liuwei Dihuang decoction senescence accelerated mo

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Association of BrERF72 with methyl jasmonate-induced leaf senescence of Chinese flowering cabbage through activating JA biosynthesis-related genes

引用

Horticulture Research 2018年第1期5卷 641-651页

作者： Xiao-li Tan Zhong-qi Fan Wei Shan Xue-ren Yin Jian-fei Kuang Wang-jin Lu Jian-ye Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Guangdong Vegetables Engineering Research Center College of HorticultureSouth China Agricultural University510642GuangzhouChina The State Agriculture Ministry Laboratory of Horticultural Plant Growth Development and Quality ImprovementCollege of Agriculture and BiotechnologyZhejiang UniversityZijingang Campus310058HangzhouChina

The ethylene response factor(ERF)and phytohormone jasmonate(JA)are reported to function in leaf senescence.The involvement of ERF in JA-mediated leaf senescence,however,needs to be elucidated.In the present work,we demonstrate a Chinese flowering cabbage ERF transcription factor(TF),BrERF72,that is associated with JA-promoted leaf senescence.Exogenous application of methyl jasmonate(MeJA)-accelerated leaf senescence of Chinese flowering cabbage,evidenced by the data that MeJA treatment led to the stronger reduction in the maximum quantum yield(Fv/Fm),photosynthetic electron transport rate(ETR),and total chlorophyll content,while significant induction in the expression of several senescence-associated genes(SAGs)including BrSAG12,BrSAG19,and chlorophyll catabolic genes(CCGs)BrPAO1,BrNYC1,BrPPH1,and BrSGR1.Increases in levels of endogenous JA and transcripts of JA biosynthetic genes BrLOX4,BrAOC3,and BrOPR3 were also found after MeJA treatment.BrERF72 was a MeJA-inducible,nucleus-localized protein,and possessed trans-activation ability.Transient overexpression of BrERF72 in tobacco leaves also promoted leaf senescence.More importantly,further experiments revealed that BrERF72 directly activated expression of BrLOX4,BrAOC3,and BrOPR3 through binding to their promoters via the GCC or DRE/CRT cis-element.Together,the novel JA-ERF association reported in our study uncovers a new insight into the transcriptional regulation of JA production mediated by ERF during JA-promoted leaf senescence in Chinese flowering cabbage.

关键词： ERF senescence Together

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

senescence and longevity in amniotic fluid derived cells

引用

Stem Cell Discovery 2013年第1期3卷 47-55页

作者： Z. Chen A. Jadhav F. Wang M. Perle R. Basch B. K. Young Department of Obstetrics & Gynecology New York University New York USA Department of Pathology New York University New York USA

Amniotic fluid stem cells(AFSC) are noncontroversial and abundant potentially transplantable cells. They are derived from fetal and amniotic sources and show osteogenic, neuronal, and adipogenic differentiation in culture. Clinical material must be expanded in culture and sorted if clinical use is desired. Cellular senescence and replicative potential for AFSC cultures has had limited study, with scant data on gene expression over time. We report changes in samples from 17 patients over multiple passages form 10 to 81 population doublings. Longevity was unrelated to telomere length in these cells. It was related to upregulation of TWIST1, which is highly expressed in stem cells, and downregulation of genes associated with apoptosis.

关键词： Amniotic Fluid Stem Cells senescence Longevity Telomeres Genes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

RhMYB108,an R2R3-MYB transcription factor,is involved in ethylene-and JA-induced petal senescence in rose plants

引用

Horticulture Research 2019年第1期6卷 70-81页

作者： Shuai Zhang Qingcui Zhao Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Nan Ma Yonghong Li School of Applied Chemistry and Biological Technology Postdoctoral Innovation Practice BaseShenzhen PolytechnicShenzhenGuangdong 518055China Shenzhen Key Laboratory of Fermentation Purification and AnalysisShenzhen PolytechnicShenzhen 518055 GuangdongChina College of Horticulture and Landscape Architecture Zhongkai University of Agriculture and EngineeringGuangzhouGuangdong 510642China China Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureChina Agricultural UniversityBeijingChina

Rose(Rosa hybrida)plants are major ornamental species worldwide,and their commercial value greatly depends on their open flowers,as both the quality of fully open petals and long vase life are important.Petal senescence can be started and accelerated by various hormone signals,and ethylene is considered an accelerator of petal senescence in rose.To date,however,the underlying mechanism of signaling crosstalk between ethylene and other hormones such as JA in petal senescence remains largely unknown.Here,we isolated RhMYB108,an R2R3-MYB transcription factor,which is highly expressed in senescing petals as well as in petals treated with exogenous ethylene and JA.Applications of exogenous ethylene and JA markedly accelerated petal senescence,while the process was delayed in response to applications of 1-MCP,an ethylene action inhibitor.In addition,silencing of RhMYB108 alter the expression of SAGs such as RhNAC029,RhNAC053,RhNAC092,RhSAG12,and RhSAG113,and finally block ethylene-and JA-induced petal senescence.Furthermore,RhMYB108 was identified to target the promoters of RhNAC053,RhNAC092,and RhSAG113.Our results reveal a model in which RhMYB108 functions as a receptor of ethylene and JA signals to modulate the onset of petal senescence by targeting and enhancing senescence-associated gene expression.

关键词： expression flowers senescence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcriptome profiling reveals regulatory mechanisms underlying corolla senescence in petunia

引用

Horticulture Research 2018年第1期5卷 711-723页

作者： Hong Wang XiaoXiao Chang Jing Lin Youhong Chang Jen-Chih Chen Michael S.Reid Cai-Zhong Jiang Institute of Pomology/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Jiangsu Academy of Agricultural Sciences210014NanjingChina Department of Plant Sciences University of CaliforniaDavisDavisCA95616USA Institute of Fruit Tree Research Guangdong Academy of Agricultural Science510640GuangzhouChina Institute of Biotechnology National Taiwan University10617TaipeiTaiwan United States Department of Agriculture Crops Pathology and Genetics Research UnitAgricultural Research ServiceDavisCA95616USA

The genetic regulatory mechanisms that govern natural corolla senescence in petunia are not well understood.To identify key genes and pathways that regulate the process,we performed a transcriptome analysis in petunia corolla at four developmental stages,including corolla fully opening without anther dehiscence(D0),corolla expansion,2 days after anthesis(D2),corolla with initial signs of senescence(D4),and wilting corolla(D7).We identified large numbers of differentially expressed genes(DEGs),ranging from 4626 between the transition from D0 and D2,1116 between D2 and D4,a transition to the onset of flower senescence,and 327 between D4 and D7,a developmental stage representing flower senescence.KEGG analysis showed that the auxin-and ethylene-related hormone biosynthesis and signaling transduction pathways were significantly activated during the flower development and highly upregulated at onset of flower senescence.Ethylene emission was detected at the D2 to D4 transition,followed by a large eruption at the D4 to D7 transition.Furthermore,large numbers of transcription factors(TFs)were activated over the course of senescence.Functional analysis by virus-induced gene silencing(VIGS)experiments demonstrated that inhibition of the expression of TFs,such as ethylene-related ERF,auxin-related ARF,bHLH,HB,and MADS-box,significantly extended or shortened flower longevity.Our data suggest that hormonal interaction between auxin and ethylene may play critical regulatory roles in the onset of natural corolla senescence in petunia.

关键词： senescence flower representing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：