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Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog

Cell Research 2003年第6期13卷 499-502页

作者： GUANG Jin PAN, DUAN QING PEI Institute of Pharmacology, Department of Biological Sciences and Biotechnology and State Key Labora tory of Biomembrane and Membrane Biotechnology, Tsinghua Institutes of Biomedicine, Tsinghua University, 100084 Beijing, China. E-mail: duanqing@aol.comDepartment of Pharmacology, University of Minnesota, Minneapolis, MN55455, USA 1Institute of Pharmacology Department of Biological Sciences and Biotechnology and State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua Institutes of Biomedicine Tsinghua University 100084 Beijing China. E-mail: duanqing@*** 2Department of Pharmacology University of Minnesota Minneapolis MN55455 USA

Nanog is a newly identified homeodomain gene that functions to sustain the pluripotency of embryonic stem cells. However, the molecular mechanism through which nanog regulates stem cell pluripotency remains unknown. Mouse nanog encodes a polypeptide of 305 residues with a divergent homeodomain similar to those in the NK-2 family. The rest of nanog contains no apparent homology to any known proteins characterized so far. It is hypothesized that nanog encodes a transcription factor that regulates stem cell pluripotency by switching on or off target genes. To test this hypothesis, we constructed fusion proteins between nanog and DNA binding domains of the yeast transcription factor Gal4 and tested the transactivation potentials of these constructs. Our data demonstrate that both regions N- and C- terminal to the homeodomain have transcription activities. Despite the fact that it contains no apparent transactivation motifs, the C-terminal domain is about 7 times as active as the N-terminal one. This unique

关键词： nanog pluripotency stem cells self renewal transactivation domain homeoprotein homeodomain

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