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The Response Difference of Mitochondria in Recalcitrant Antiaris toxicaria Axes and Orthodox Zea mays Embryos to Dehydration Injury

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Journal of Integrative Plant Biology 2009年第7期51卷 646-653页

作者： Song-Quan Song Mei-Hua Tian Jing Kan Hong-Yan Cheng Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Xishuangbanna Tropical Botanical Garden the Chinese Academy of Sciences Mengla 666303 China

Long-term preservation of recalcitrant seed. is very difficult because the physiological basis on their desiccation sensitivity is poorly understood. Survival of Antiaris toxicaria axes rapidly decreased and that of immature maize embryos very slowly decreased with dehydration. To understand their different responses to dehydration, we examined the changes in mitochondria activity during dehydration. Although activities of cytochrome （Cyt） c oxidase and malate dehydrogenase of the A. toxicaria axis and maize embryo mitochondria decreased with dehydration, the parameters of maize embryo mitochondria were much higher than those of A. toxicaria, showing that the damage was more severe for the A. toxicaria axis mitochondria than for those of maize embryo. The state I and III respiration of the A. toxicaria axis mitochondria were higher than those of maize embryo, the former rapidly decreased, and the latter slowly decreased with dehydration. The proportion of Cyt c pathway to state III respiration for the A. toxicaria axis mitochondria was low and rapidly decreased with dehydration, and the proportion of alternative oxidase pathway was high and slightly increased with dehydration. In contrast, the proportion of Cyt c pathway for maize embryo mitochondria was high, and that of alternative oxidase pathway was low. Both pathways decreased slowly with dehydration.

关键词： alternative oxidase pathway cytochrome c oxidase pathway dehydration mitochondria orthodox recalcitrance respiration rate seed.

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In vitro evaluation of native entomopathogenic fungi and neem(Azadiractha indica)extracts on Spodoptera frugiperda

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 47-54页

作者： Hernandez-Trejo A B Estrada-Drouaillet JA Lopez-Santillan C Rios-Velasco SE Varela-Fuentes R Rodriguez-Herrera E Osorio-Hernandez Postgraduate Studies and Research Division Autonomous University of TamaulipasSchool of Engineering and Sciences.Centro Universitario Adolfo Lopez MateosCd.VictoriaTamaulipasC.P.87000Mexico Center for Research in Food and Development A.C.Cuauhtemoc Unit.Laboratory of Postharvest PhysiologyPlant Pathology and Biological Control.Av.Rio Conchos s/n Industrial ParkCd.CuauhtemocChihuahuaC.P.31570Mexico School of Chemistry Autonomous University of CoahuilaBlvd.V.Carranza y Jose Cardenas s/nSaltilloCoahuilaC.P.25000Mexico

The control of Spodoptera frugiperda is based on synthetic insecticides,so some alternatives are the use of entomopathogenic fungi(EF)and neem *** objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on *** *** EF were identified by DNA sequencing of ITS regions from three EF(Fusarium solani,Metarrhizium robertsii,Nigrospora spherica and Penicillium citrinum).They were evaluated in concentrations of 1×10⁸spores/*** addition,a second bioassay was carried out evaluating only ***,*** and *** and the addition of vegetable *** the other hand,extraction of secondary metabolites from neem seed.Azadirachta indica)was carried out by performing,mass(g)and solvent volume(mL ethanol and water)combinations,which were subjected to microwaves and ***,these extracts were evaluated in concentrations of 3%,4%and 5%.A survival analysis was performed for each of the *** respect to the results of the first bioassay,*** obtained a probability of survival of 0.476 on the seventh day,while in the second bioassay,*** obtained 0.488 survival *** suggests that the expected percentage of larvae that stay alive on the sixth day is 48.8%.However,in the evaluation of the neem extract the combination 1:12/70%to 4%caused 84% mortality of *** use of native HE and neem extracts has potential for the control of ***.

关键词： Bioinsecticide Biological control Fall armyworm Extracts seed.

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Promotion of IAA, NAA on seed.Germination of Jacaranda mimosifolia

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Journal of Agricultural Science and Technology(B) 2012年第11期2卷 1184-1189页

作者： Fan Li Linyun Zhou Jin'an Shi Suping Gao College of Landscape Architecture Sichuan Agricultural University Chengdu 611130 People's Republic of China

seed.germination of Jacaranda mimosifolia treated with Indoleacetic Acid （IAA） and Naphthylacetic Acid （NAA） was investigated. seed. were collected from 55-year-old plants in March 2012 in Chengdu Wenjiang Park in China and sown on illumination incubator at 25 ℃/18℃ （day/might） under 15 h light after soaked 24 h with 0, 50, 100 and 200 mg L-1 IAA, NAA solutions. The thousand seed.weight ofJ. mimosifolia was 9.039 g and seed.average vigor was 32.7%. Germination rate was 13.3% without treatment, and seed. with 50, 100 and 200 mg L-1 IAA treatment showed germination rate of 20.0%, 22.3% and 25.0%, respectively, whereas the rate was 18.3%, 20.0% and 23.3% in 50, 100 and 200 mg L-1 NAA treatment. There was significant difference between 100, 200 mg L-1 and other IAA treatment, but only 200 mg L-1 NAA treatment had significant difference among others. It showed that IAA could promote seed.germination more significantly than NAA in the same concentration conditions. These results indicate that soaking by 100 and 200 mg L-1 IAA or 200 mg L-1 NAA for 24 h, seed.germination rate ofJ. mimosifolia can be significantly improved and robust seed.ings obtained from germinated seed..

关键词： Jacaranda mimosifolia germination IAA NAA seed.

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seed.Germination and Dormancy for Iris Chrysographes （Iridaceae） from China

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Journal of Agricultural Science and Technology(A) 2013年第6期3卷 498-502页

作者： Xiaofang Yu Zhuo Huang Yin Jia Lingxia Sun Guangli Liu Huixing Song Yonghong Zhou Landscape Architecture College Triticeae Research Institute Sichuan Agricultural University Wenjiang 611130 Chengdu SichuanChina

Several Chinese Iris species have potential for medicinal and ornamental use, but they have poor, erratic or slow seed.germination. The aims of this study was to classify Iris ehrysographes dormancy type and investigate the optimum treatment for seed. germination through examining the effects of various incubation temperatures, light conditions, scarification, stratification and soil substratum. seed.viability of/. chrysographes was 74% and seed. imbibed water very fast. No seed. germinated on stratification, soil substratum, constant or alternating temperatures, light and darkness treatments. seed. germination of different mechanical restriction treatments had significant differences. The results indicated that：（1） removal of seed.coat was the most effective treatment for/. chrysographes germination; （2） physiological dormancy phenomenon was found in this species.

关键词： Dormancy germination Iris chrysographes seed.

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