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Journal of Forestry Research 2022年第4期33卷 1365-1375页

作者： Cecilia Laurell Torkel Berglund Anna B.Ohlsson Department of Industrial Biotechnology School of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden

Nicotinamide treatment of plants and plant cell cultures has been shown to promote defense and decrease levels of DNA *** the present study,we used RNA-seq technology to study overall changes in gene expression induced in roots of 3-month-old spruce(Picea abies)seedlings grown from nicotinamide-treated seeds to examine the molecular mechanisms underlying the defense *** 350 genes were identified as differentially expressed in roots after the seed *** response genes,including transcription factors MYB77 and LHY and two chitinase enzymes,were generally upregulated,whereas genes thought to be involved in epigenetic regulation such as DDM1,known to promote DNA methylation,were present at high frequency among the downregulated *** all samples,the expression of downregulated epigenetic-related genes was highly correlated with the nicotinamide treatment,indicating a common *** results support an earlier hypothesis regarding a potential role of nicotinamide as a defense-signal mediator.

关键词： DNA methylation Large-scale gene expression analysis Nicotinamide Picea abies seed treatment

Effect of seed treatment for laboratory germination of Albizia chinensis

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Journal of Forestry Research 2013年第4期24卷 709-713页

作者： A.Nongrum L.Kharlukhi seed Physiology Laboratory Botany DepartmentNorth-Eastern Hill University

seeds of Albizia chinensis（Osb.） Merr. in addition to water were also treated with different treatments by incubating in ethyl alcohol, acetone, and petroleum ether at room temperature for different durations. seed heat treatment was done at temperatures of 30, 40 and 60℃ for different durations up to 24 h. To overcome dormancy caused by the impermeable seed coat, seeds were nicked and also treated with concen- trated sulphuric acid for different durations. seeds responded to treat- ments with sulphuric acid and nicking only. treatment with sulphuric acid for 20 and 30 min showed maximum germination at all incubation temperatures as compared to untreated controls and seeds treated with sulphuric acid for 10 min and nicking. seedling length was greatest from seeds treated with sulphuric acid for 20 and 30 min and incubated at 30 ℃. seedling dry weight was highest from nicked seeds incubated at 20℃ The most favourable incubation temperature was 30 ℃ as evidenced from GR （germination rate index） and Gv （germination value）. After ascertaining the seed response and performance we recommend that seeds ofAlbizia chinensis be treated with sulphuric acid for 20 or 30 min and incubation temperature of 25 to 30℃.

关键词： Germination Rate Index Germination Value seed treatment

seed treatment and inhibition of plantpathology by chitosan

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Journal of Environmental Sciences 1994年第1期6卷 112-115页

作者： Jiang Tingda Ma Ruixia Qi Ruinung Zhang Chunping(Researeh Center for Eco-Environmental Sciences, Chinese Aeademy of Sciences.)Beijing 100085, China

0. 1% solution of chitosan was studied as seed soaking agent for cotton and *** will help stimulate the seeds of cotton and maize for sprouting. It can also restrain thefosanum oxysporin and verticillium. then inhibit the cotton wilt and maize smut. The annual out-put of cotton and maize increased by 11.80% and ,0.4%, respectively.

关键词： chitosan cotton maize seed treatment

Biocontrol of Rhizoctonia solani K1 by Iturin A Producer Bacillus subtilis RB14 seed treatment in Tomato Plants

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Advances in Microbiology 2016年第6期6卷 424-431页

作者： Umme Salma Zohora Takashi Ano Mohammad Shahedur Rahman Department of Biotechnology and Genetic Engineering Faculty of Biological Sciences Jahangirnagar University Dhaka Bangladesh Department of Biotechnological Science Faculty of Biology-Oriented Science and Technology Kinki University Kinokawa-city Japan

Bacillus subtilis RB14 was used as an antagonist against fungal pathogen Rhizoctonia solani K1 to control damping-off diseases in tomato plants. Tomato seeds were treated with B. subtilis RB14 culture. The concentration of bacterial cells for the treatment was about 108 cfu/ml. Treated tomato seeds showed 99% germination index similar to the untreated seeds. Scanning Electron Microscopic observations showed a clear evidence of the presence of B. subtilis RB14 on tomato seed surface. Clear inhibition zone was observed using treated seed in dual plate assay against R. solani K1. B. subtilis RB14 treated seed showed 80% reduction in disease incidence during in vivo plant experiments. B. subtilis RB14 produces lipopeptide antifungal antibiotic iturin A which could suppress R. solani K1. The phenomenon was supported by our observation where we found significant amount of iturin A from the root zone soil of the seed treated plants.

关键词： Bacillus subtilis RB14 Rhizoctonia solani K1 seed treatment Iturin A

Comparative research of effective utilization and efficacy duration of phenamacrilon rice bakanae disease control by different seed treatment methods Department of Plant Pathology,China Agricultural University

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Comparative research of effective utilization and efficacy d...

Effect of Cold Plasma treatment on seed Germination and Growth of Wheat

中国植物病理学会2017年学术年会

作者： Liu Panqing Wang Mingqi Liu Pengfei Department of Plant Pathology China Agricultural University

Phenamacril is a domestically created new fungicide,which showed a good control effecton rice bakanae *** clarify the effective utilization and efficacy duration of phenamacril on rice bakanae disease control,the flowable concentrate for seed coating(FSC) and flowable concentrate for seed treatment(FS) of phenamacril were prepared and used by seed *** residue of phenamacril on seeds after soaking was studied by means of QuEChERS——High performance liquid chromatography(HPLC).The sample(3 g) was extracted with 1%acetic acid acetonitrile(3 mL),purified by graphite carbon black(GCB)(7.5 mg/mL).The solution was detected by HPLC with UV detection at290 *** linear regression formulations of phenamacril in seed and plant were y= 41.106 0 x-2.722 4(R=0.999 5) and y = 45.739 x-26.764(R=0.999 4) *** recoveries were ranged from 88%to 115%.The results showed that the method of quantitative determination was of a high accuracy and *** showed that there was no significant difference in pesticide utilization between seed soaking with water after coated with FSC and seed soaking with *** degradation dynamics study of phenamacril in rice plants used by seed coating was in conformity with the first-order dynamic *** decline coefficient was K = 0.085 5,T1/2 = *** and a residue of 2.78 mg/kg could be detected after 25 days of *** residue is quite more than the EC of phenamacril on Fusarium fujikuroi in vitrowhich shows the efficacy duration on rice bakanae disease can be more than 25 d in *** study may offer an effective reference for the research and development of phenamacril by seed coating.

关键词： Phenamacril seed treatment QuEChERS HPLC

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Plasma Science and Technology 2014年第1期16卷 54-58页

作者：蒋佳峰何昕李玲李建刚邵汉良徐启来叶仁宏董元华 Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil ScienceChinese Academy of Sciences University of Chinese Academy of Sciences Changzhou Zhongke Changtai Plasma Technology Corporation Jiangsu Provincial Agricultural Reclamation and Development Corporation Agricultural Center of Jiangsu Xinyang Farm

This study investigated the effect of cold helium plasma treatment on seed germina- tion, growth and yield of wheat. The effects of different power of cold plasma on the germination of treated wheat seeds were studied. We found that the treatment of 80 W could significantly improve seed germination potential （6.0%） and germination rate （6.7%） compared to the control group. Field experiments were carried out for wheat seeds treated with 80 W cold plasma. Com- pared with the control, plant height （20.3%）, root length （9.0%） and fresh weight （21.8%） were improved significantly at seedling stage. At booting stage, plant height, root length, fresh weight, stem diameter, leaf area and leaf thickness of the treated plant were respectively increased by 21.8%, 11.0%, 7.0%, 9.0%, 13.0% and 25.5%. At the same time, the chlorophyll content （9.8%）, nitrogen （10.0%） and moisture content （10.0%） were higher than those of the control, indicating that cold plasma treatment could promote the growth of wheat. The yield of treated wheat was 7.55 t-ha-1, 5.89% more than that of the control. Therefore, our results show that cold plasma has important application prospects for increasing wheat yield.

关键词： cold plasma seed treatment wheat seed germination plant growth increasedyield

seed Priming with MgCl_(2),CaCl_(2),and ZnCl_(2) as a Biofortification Based-Approach Induces Changes in Anise seedlings Emergence

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Phyton-International Journal of Experimental Botany 2023年第8期92卷 2461-2471页

作者： Sibel Day Nilüfer Koçak-Şahin Department of Field Crops Faculty of AgricultureAnkara UniversityAnkara06110Türkiye

Aromatic and medicinal plant species having small seeds haveﬁeld emergence problems due to low nutrient ***,Pimpinella anisum seeds were hydro and osmoprimed with 100 mM MgCl_(2),CaCl_(2),and ZnCl_(2),for 2,4,and 8 h each to compare their growth attributes during germination and seedling establishment *** seeds were used as *** hydro and osmo primed seeds were dried for 48 h before,they were sown in plastic trays in growth room conditions to see the impact of treatments on seedling emergence and *** maximum root length(12.90 cm),fresh weight(256.30 mg plant^(-1)),and mean emergence time(MET)were recorded from 8 h MgCl_(2) primed ***,the maximum(97.50,and 98.00%)emergence percentage was observed using 8 h MgCl_(2) primed seeds and nontreated seeds(control treatment).The evaluation of parameters like chlorophyll contents and electrical conductivity showed the 8 h MgCl_(2) priming as the optimum *** evaluation of parameters like chlorophyll contents and electrical conductivity showed the 8 h MgCl_(2) priming as the optimum *** result suggests MgCl_(2) priming worked synergistically and improved seed-ling growth attributes under greenhouse *** chlorophyll content ranged 25.94–35.69 SPAD *** highest chlorophyll content was obtained from the seedlings obtained from 4 h CaCl_(2) treatment,which were statistically similar to the chlorophyll contents of the seedlings obtained after 8 h MgCl_(2) treatment and nontreated seeds(control treatment).All other treatments showed inhibition in the chlorophyll contents and growth attributes of the *** conclusion,MgCl_(2) osmopriming treatments were signiﬁcantly promotive and better compared to hydro-priming and osmopriming treatments including control treatment in terms of anise seeds germination and emergence.

关键词： Pimpinella anisum L seed treatment hydropriming osmopriming electrolytes leakage