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Journal of Integrative Plant Biology 2024年第3期66卷 468-483页

作者： Shuanghe Cao Bingyan Liu Daowen Wang Awais Rasheed Lina Xie Xianchun Xia Zhonghu He State Key Laboratory of Crop Gene Resources and Breeding/National Wheat Improvement Center Institute of Crop SciencesBeijing 100081China College of Agronomy Henan Agricultural UniversityZhengzhou 450002China International Maize and Wheat Improvement Center(CIMMYT)China Office Chinese Academy of Agricultural SciencesBeijing 100081China

Achieving high yield and good quality in crops is essential for human food security and health.However,there is usually disharmony between yield and quality.seed storage protein(SSP)and starch,the predominant components in cereal grains,determine yield and quality,and their coupled synthesis causes a yield–quality trade-off.Therefore,dissection of the underlying regulatory mechanism facilitates simultaneous improvement of yield and quality.Here,we summarize current findings about the synergistic molecular machinery underpinning SSP and starch synthesis in the leading staple cereal crops,including maize,rice and wheat.We further evaluate the functional conservation and differentiation of key regulators and specify feasible research approaches to identify additional regulators and expand insights.We also present major strategies to leverage resultant information for simultaneous improvement of yield and quality by molecular breeding.Finally,future perspectives on major challenges are proposed.

关键词： cereal crops grain yield and quality seed storage protein starch synergistic regulation

Regulation of seed storage protein synthesis in monocot and dicot plants:A comparative review

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Molecular Plant 2023年第1期16卷 145-167页

作者： Tao Yang Xingguo Wu Wenqin Wang Yongrui Wu National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200233China

seeds are a major source of nutrients for humans and animal livestock worldwide.With improved living standards,high nutritional quality has become one of the main targets for breeding.storage protein content in seeds,which is highly variable depending on plant species,serves as a pivotal criterion of seed nutritional quality.In the last few decades,our understanding of the molecular genetics and regulatory mechanisms of storage protein synthesis has greatly advanced.Here,we systematically and comprehensively summarize breakthroughs on the conservation and divergence of storage protein synthesis in dicot and monocot plants.With regard to storage protein accumulation,we discuss evolutionary origins,developmental processes,characteristics of main storage protein fractions,regulatory networks,and genetic modifications.In addition,we discuss potential breeding strategies to improve storage protein accumulation and provide perspectives on some key unanswered problems that need to be addressed.

关键词： maize rice Arabidopsis seed storage protein nutritional quality transcription factor

Characterization of Agro-diversity by seed storage protein Electrophoresis:Focus on Rice Germplasm from Uttarakhand Himalaya,India

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Rice science 2010年第2期17卷 122-128页

作者： Arun JUGRAN Indra D.BHATT Ranbeer S.RAWAL G.B.Pant Institute of Himalayan Environment and Development Kosi-Katarmal

The characteristics of 48 rice varieties from Uttarakhand Himalaya, India were detected by morphological and biochemical markers. The grains of the selected rice varieties varied in their morphological （grain length, grain width and grain weight） and biochemical characters （sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE）. Based on the presence of 70, 65, 60, 57, 37-39, 22-23, 13 and 10 kDa protein bands in the 48 rice varieties, seven types of profiles were identified. An unweighted pair group average method with arithmetic mean （UPGMA） dendrogram based on cluster analysis of genetic similarity of the protein bands showed two distinct groups with 1%-78% similarity coefficients. The presence of characteristic bands in selected varieties is a useful parameter for identification of rice germplasm.

关键词： rice seed storage protein sodium dodecyl sulfate polyacrylamide gel electrophoresis genetic diversity polymorphism varietal identification

Genetic Diversity and Classification of Chinese Elite Foxtail Millet [Setaria italica (L.) P. Beauv.] Revealed by Acid-PAGE Prolamin

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Agricultural Sciences 2022年第3期13卷 404-428页

作者： Guoxing Ma Qiang Li Suying Li Zhengli Liu Yanjiao Cui Jing Zhang Dan Liu Department of Life Sciences Tangshan Normal University Tangshan China Tianjin Key Laboratory of Crop Genetics and Breeding Institute of Crop Sciences Tianjin Academy of Agricultural Sciences Tianjin China

Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability.

关键词： Foxtail Millet [Setaria italica (L.) P. Beauv.] seed storage protein protein Polymorphism Prolamin

Effect of Allelic Variation of β-conglycinin α-subunit Locus cgy-2 on Soybean(Glycine max) Quality Ⅰ. Evaluation of Free Amino Acid Content

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Journal of Northeast Agricultural University(English Edition) 2022年第1期29卷 9-25页

作者： Qiu Zhen-dong Luo Ting-ting Song Yan-ru Ma Chong-xuan Tian Yu-su Zhou Jin-tao Song Bo Liu Shan-shan Key Laboratory of Soybean Biology in Chinese Ministry of Education(Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry) Northeast Agricultural UniversityHarbin 150030China

The effects of the deficiency of the allergenicα-subunit on soybean amino acid(AA)composition were studied using the cultivar Dongnong 47(DN47)as a genetic background.The near-isogenic line(NIL)NIL-DN47-Δα of DN47,with an introgression of theα-null trait allele from the high protein donor parent RiB,was created by marker assisted background selection and used to investigate the AA content and nutritional quality.The contents of crude protein,the total AAs,the total essential amino acids(EAAs)and sulfur-containing(Met and Cys)AAs increased by 4.11%,4.16%,5.20% and 11.96%,respectively in NIL-DN47-Δα compared with DN47.Analyses of the total EAAs(TEAAs)and the EAA index(EAAI)revealed that both parameters in NIL-DN47-Δα were higher than those in DN47.The null-allele of theα-subunit positively affected the AA scores.The quantitative changes in free AAs(FAAs)in the developing seeds of NIL-DN47-Δα and DN47 were compared as of 15 days after flowering(DAF)until maturity.The results showed that the total FAA content in NIL-DN47-Δα was significantly higher than that in the DN47 throughout the late maturation stage(40-60 DAF)of seeds.The high concentration of the FAAs in cgy-2 mutant seeds was a consequence of the high rates of synthesis and/or accumulation of individual FAAs during seed maturation where 25 DAF was an important turning point in the accumulation of the FAAs.The FAA contents of single soybeanα-null,double(α+α′)and triple(α+α′+group I)-null mutant combination lines were investigated.In all of these combinations,introduction of the cgy-2 gene invariably raised the FAA content of mature seeds above that of the DN47.In summary,the enhanced protein quality in cgy-2 mutants resulted from several factors.(1)There was a general increase in the contents of most AAs and FAAs in NIL-DN47-Δα.(2)The induced synthesis of free Arg contributed effectively to the high FAAs of various storage-protein-deficiency mutants.For example,in the S2(null α,group I),the free Arg content was seven times as much as that of DN47,accounting f

关键词： soybean(Glycine)β-conglycinin α-subunit null mutant free amino acid arg-overproducing seed storage protein

Disulphide Linkages Occur in Many Polypeptides of Rice protein Fractions: a Two-dimensional Gel Electrophoretic Study

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Rice science 2011年第2期18卷 86-94页

作者： Arvinder SINGH Narender K.MATTA Department of Botany Kurukshetra University

Most of the work on rice storage proteins focused on the major fraction - glutelin. In contrast to previous reports of the occurrence of single legumin-like polypeptide pair in glutelin, two-dimensional gel electrophoresis revealed many glutelin bands, with some having and some lacking disulphide-linkages. Five legumin-like polypeptide pairs exhibited wide heterogeneity over a range of molecular weight （Mr） 25 to 60 kDa, each having a large subunit （Mr ranged from 18 to 40.5 kDa） disulphide-bonded to a small subunit （Mr ranged from 16.5 to 18.0 kDa）. A band of 49 kDa was homodimeric with two subunits of 29 kDa each; a polypeptide of 51 kDa which altered position to ones corresponding to 53 kDa and 57 kDa on 2-D gels contained intrapolypeptide linkages. Polypeptides of 65 kDa and 60 kDa occurred as aggregates of 110 kDa. The number of polypeptides in other seed protein fractions albumins, globulins and prolamins, varied from four to ten. One of the albumin bands had intrapolypeptide disulphide linkages （20 kDa） and the globulins contained two such bands （13.5 and 20 kDa）. Thus, the present study provides a description of the polypeptide composition of different rice protein fractions that is finely resolved with respect to the occurrence of disulphide linkages.

关键词： seed storage protein glutelin prolamin globulin Oryza sativa

Expression Regulation of seed storage proteinand Starch Synthesis in Common Wheat

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Expression Regulation of Seed Storage Proteinand Starch Synt...

Comparison of seed storage proteins of wild rice species with the cultivated rice

第八届全国小麦基因组学及分子育种大会

作者： Lisha Shen Guangbin Luo Yanhong Song Shuyi Song Wenlong Yang Jiazhu Sun Kehui Zhan Aimin Zhang Dongcheng Liu State Key Laboratory of Plant Cell and Chromosome Engineering National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of sciences College of Agronomy The Collaborative Innovation Center of Grain Crops in HenanHenan Agricultural University

seed storage proteins(SSPs) and starch are main components in wheat grain,and their contents and compositions determine the flour processing quality and yield,respectively.The expression levels of SSP genes and starch synthesis related genes(SSRGs) are positively correlated with the final accumulation of SSPs and starch.However,the process of SSP and starch synthesis was poorly understood,and only a few transcription factors(TF,e.g.bZIP,R2 R3 MYB and Dof) have been characterized.In this work,the transcriptome profiling of maturing endosperms[5,10,15,20 and 25 days post anthesis(DPAs)]in Chinese Spring were investigated though both RNA-Seq and miRNA-Seq platforms.During the grain-filling stages,the expression of SSP genes initiated at 5 DP A,peaked at 20 DP A,and then declined gradually.SSRGs shared similar expression model,which started at 5 DP A,and reached at their peaks at 10 DP A and then decreased,but the accumulation of amylose and amylopectin had different patterns along with the grain filling process.In the RNA-Seq assay,74 TFs were observed co-expressed with SSP genes and SSRGs,while 61 miRNAs had similar expression pattern with SSP genes and SSRGs according to the miRNA-Seq data.These TFs and miRNAs might regulate the SSP gene and SSRG expression due to their co-existence in the filling wheat grains.Intriguingly,except these previously reported bZIP,R2 R3 MYB and Dof,much more gene families might involve in the SSP and starch synthesis,e.g.bHLH,AIL5,ESR1,MADS,DRFL,GL9 H2,KAN2 and WRKY,though a detailed work on these genes was necessary in the future.Furthermore,nine novel miRNAs were also characterized.These data would provide the fundamental information for us to understand the SSP and starch synthesis in common wheat.

关键词： seed storage protein Starch synthesis Transcription fector miRNA Expression regulation

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Comparison of seed storage proteins of wild rice species wit...