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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 427-433页

作者： Ya-jing GUAN Jin HU Xian-ju WANG Chen-xia SHAO seed Science Center College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Yuan Longping High-Tech Agriculture Co. Ltd. Changsha 410001 China

Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize （Zea rnays L.） inbred lines, HuangC （chilling-tolerant） and Mo17 （chilling-sensitive）. While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time （MGT）, and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde （MDA） content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase （POD） activity, and catalase （CAT） activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. priming with 0.50% chitosan for about 60-64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.

关键词： seed priming Chitosan Low temperature stress Germination Physiological changes Maize

seed priming Improves Enzymatic and Biochemical Performances of Rice During seed Germination under Low and High Temperatures

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Rice science 2023年第4期30卷 335-347,I0024-I0027页

作者： Salar MONAJJEM Elias SOLTANI Ebrahim ZAINALI Masoud ESFAHANI Farshid GHADERI-FAR Maryam HOSSEINI CHALESHTORI Atefeh REZAEI seed Science and Technology Department of AgronomyUniversity of GuilanRasht 41996-13776Iran Department of Agronomy University of TehranTehran 33916-53755Iran Department of Agronomy Gorgan University of Agricultural Sciences and Natural ResourcesGorgan 49138-15739Iran Rice Research Institute of Iran Rasht 41996-13475Iran Food and Drug Administration Guilan University of Medical ScienceRasht 41937-1311Iran

As an abiotic stress,adverse germination temperatures cause serious disruptions in physiological and biochemical processes involved in seed *** a factorial experiment,we examined the effects of different seed priming treatments on enzymatic and biochemical performances of rice seed germination under different *** of the rice genotypes(Hashemi,Sadry-domsefid,IRON-70-7053-7 and NORIN-22)was primed with hydro-hardening,KCl,CaCl2 and ascorbic acid(AsA)and without a priming agent as a control at low(15℃),optimum(25℃)and high(35℃)germination *** results showed that the enzymatic and biochemical performances of all the rice genotypes were affected by the seed priming agents,especially under the low germination *** 15℃,seed priming with AsA was found to be the best agent for the activities of amylase,α-amylase,catalase(CAT),peroxidase(POX),ascorbate peroxidase(APOX)and superoxide dismutase(SOD)as well as the content of soluble sugars in the NORIN-22 genotype,and for protease activity and soluble protein content in the IRON-70-7053-7 *** at the low germination temperature and CAT,POX and protease at the optimum and high germination temperatures were the most important enzymes in occurrence of germination potential in terms of seedling length,vigor index,normal seedling rate and germination *** the priming agents,the highest changes in normal seedling rate were observed at the low and optimum germination temperatures by AsA priming in the Hashemi and NORIN-22 genotypes,and at the high germination temperature under KCl priming in the Hashemi genotype.

关键词： antioxidant activity biochemical performance germination temperature rice seed priming

Influence of seed priming on Performance and Water Productivity of Direct seeded Rice in Alternating Wetting and Drying

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Rice science 2015年第4期22卷 189-196页

作者： Hafeez Ur REHMAN Muhammad KAMRAN Shahzad Maqsood Ahmed BASRA Irfan AFZAL Muhammad FAROOQ Department of Agronomy University of AgricultureFaisalabad Institute of Agriculture University of Western Australia College of Food and Agricultural Sciences King Saud University

Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of seed priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct seeded rice in alternate wetting and drying （DSR-AWD） in comparison with direct seeded rice at field capacity （DSR- FC）. seed priming treatments were osmo-priming with KCI （2.2%）, CaCI2 （2.2%） and moringa leaf extracts （MLE, 3.3%） including hydro-priming as control. Among the treatments, seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-priming with CaCI2. Tillering emergence rate and number of tillers per plant were the highest for seed priming with CaCI2 in DSR- AWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for seed priming with MLE or CaCI2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for seed priming with MLE. In conclusion, seed priming with MLE or CaCI2 can be successfully employed to improve the direct seeded rice performance when practiced with alternate wetting and drying irrigation.

关键词： crop establishment grain filling rate seed priming water productivity yield direct seededrice alternating wetting and drying grain quality

seed priming Improves Agronomic Trait Performance under Flooding and Non-flooding Conditions in Rice with QTL SUB1

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Rice science 2012年第4期19卷 286-294页

作者： Ramni Kumar SARKAR Central Rice Research Institute Cuttack-753 006OrissaIndia

Farmers in South East Asia are adopting rice crop establishment methods from transplanting to direct wet or dry seeding as it requires less labour and time and comparatively less energy than transplanting. In contrast to irrigated condition, in rainfed lowland, direct seeding is a common practice. Early flooding controls weeds but decreases seedling establishment in direct seeded rice. Anaerobic germination is an important trait to counteract damages caused by early flooding. Management options which can help in crop establishment and improve crop growth under flooding might remove the constraints related to direct seeding. The investigation was carried out with two near isogenic lines Swarna and Swarna- Sub1. Swarna-Subl is tolerant to submergence whereas Swarna is susceptible. seed priming was done with water and 2% Jamun （Syzygium cumini） leaf extract, and it improved seedling establishment under flooding. Acceleration of growth occurred due to seed pretreatment, which resulted longer seedling and greater accumulation of biomass. seed priming greatly hastened the activities of total amylase and alcohol dehydrogenase in Swarna-Subl than in Swarna. Swarna-Subl outperformed Swarna when the plants were cultivated under flooding. Weed biomass decreased significantly under flooding compared to non-flooding conditions. seed priming had positive effects on yield and yield attributing parameters both under non-flooding and early flooding conditions.

关键词： submergence tolerance flooding rice seed priming SUB1 yield

Effect of seed priming with different concentrations of potassium nitrate on the pattern of seed imbibition and germination of rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2017年第3期16卷 605-613页

作者： Anisa Ruttanaruangboworn Wanchai Chanprasert Pitipong Tobunluepop Damrongvudhi Onwimol Department of Agronomy Faculty of Agriculture Kasetsart University Bangkok 10900 Thailand

Low germination and vigor of rice seed associated with dry-seed broadcasting are common problems encountered by rice *** objectives of this study were to evaluate the role of potassium nitrate（KNO3）on the pattern of seed imbibition and to determine the effect of seed priming with KNO3 on the germination percentage,speed and uniformity of germination in rice *** 1 compared the patterns of seed imbibition of six concentrations of KNO3（0,0.25,0.50,1.00,1.50,and 2.00%）in two rice cultivars-KDML105 and *** results showed that soaking rice seed in KNO3 at higher concentrations could delay the imbibition *** higher concentrations of KNO3 delayed the imbibition time of rice seed and took a longer time to reach the end of phases 1 and 2 compared to the lower *** patterns of seed imbibition using distilled water of both rice cultivars（KDML105 and RD15）were quite similar,but with different concentrations of KNO3,the imbibition time taken to reach the end of phases 1 and 2 was slightly postponed in KDML105 suggesting that different rice cultivars may need different imbibition times for soaking seed in the priming *** 2 evaluated the effects of seed priming with 1.0 and 2.0%KNO3 at different imbibition *** was found that priming with 1.0%KNO3 showed better seed germination than priming with 2.0%KNO3 and seed priming with 1.0%KNO3 at the imbibition time of early phase 2（or 28 h for KDML105）improved seed germination and increased both the speed and uniformity of seed *** results of this study show promise for the use of priming with 1.0%KNO3 soaked until early phase 2 of seed imbibition for improving the seed germination and vigor of rice in dry seed broadcasting.

关键词： rice water uptake seed priming KNO3 solution germination percentage speed of germination uniformity of germination

Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine

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Chilling tolerance in Nicotiana tabacum induced by seed prim...

Chitosan seed priming Improves the Yield and the Root System of Faba Bean(Vicia faba L.)Short Title:Chitosan Impact on Yield of Faba Bean

第二届全国种子科学与技术学术研讨会

作者： Shengchun Xu1,Jin Hu 1,Yongping Li2,Wenguang Ma2,Yunye Zheng2,Yajing Guan1 (1 seed Science Center,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310058,China 2 Southern Center of China Tobacco Breeding Research,Yunnan Tobacco Science Research Institute,Yuxi,653100,China)

Chilling stress is one of primary constraints to tobacco production in many parts of the *** present study was conducted to induce chilling tolerance in tobacco by seed priming with putrescine(Put) in relation to physiological changes,using seeds from two tobacco varieties,MSk326(chilling sensitive variety) and Honghuadajinyuan(HHDJY,chilling tolerant variety) .seed germination,seedling antioxidant enzyme activities and malondialdehyde(MDA) concentration,as well as polyamine concentration were determined under low *** chilling stress at 11oC,seed priming with 0.01 mM Put for 48 h(Put0.01mM48h) and seed priming with 0.1 mM Put for 48 h(Put0.1mM48h) significantly increased germination percentage,germination index,seedling length and dry weight of both varieties compared to the controls without Put *** seedlings of four-leaf stage suffered a short chilling stress(5oC) ,Put0.1mM48h improved the activities of antioxidant enzymes including superoxide dismutase(SOD) ,peroxidase(POD) ,catalase(CAT) and ascorbate peroxidase(APX) ,increased endogenous Put,Spd and Spm concentration and decreased the MDA *** results showed that Put priming treatments were available to enhance the chilling tolerance of tobacco *** optimal treatment of Put was Put0.1mM48h.

关键词： Antioxidant enzymes Chilling Polyamine seedling seed priming

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Journal of Agricultural Science and Technology(B) 2021年第2期11卷 53-60页

作者： Vahideh Rabani Hamed Cheatsazan Department of R&D AmiroyArc-lès-Gray 70100France

Faba bean is an important legume crop with food and feed *** 2015,the culture of faba bean has decreased in *** yield caused by climate change is proposed as one of the main *** is a promising biopolymer with empirically proved impact on germination and disease tolerance of faba ***,the impact of chitosan seed priming on germination and yield of this plant is not experimentally *** followed chitosan primed faba bean seeds,from sowing until maturation,using germination,fruit and grain *** observed that chitosan seed priming ameliorated several yield related *** rate,length of radicle and seedling,radicle dry matter,number of pods/plants,number of grains/pods were increased in chitosan primed faba *** grain mass/plant increased up to 47%in treated *** probable underlying mechanisms,namely,root enhancement nitrogen metabolism,and elicitation of immune system are *** conclude that,chitosan seed priming with compatible commercial preparations could be a potential solution to recompensate the loss of yield under variable climate conditions.

关键词： Faba bean chitosan seed priming germination yield

Mepiquat chloride priming confers the ability of cotton seed to tolerate salt by promoting ABA-operated GABA signaling control of the ascorbate–glutathione cycle

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Journal of Cotton Research 2023年第4期6卷 288-305页

作者： QI Qian WANG Ning RUAN Sijia MUHAMMAD Noor ZHANG Hengheng SHI Jianbin DONG Qiang XU Qinghua SONG Meizhen YAN Gentu ZHANG Xiling WANG Xiangru National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Institute of Western Agriculture the Chinese Academy of Agricultural sciencesChangji 831100XinjiangChina

Background Ensuring that seeds germinate and emerge normally is a prerequisite for cotton production,*** areas with salinized *** with mepiquat chloride(MC)can promote seed germination and root growth under salt stress,but its mechanism has not been fully *** this study,physiological and biochemical experiments revealed that MC-priming promotes the tolerance of cotton seeds to salt stress by increasing the ability of antioxidant enzymes related to the ascorbate-glutathione(AsA-GSH)cycle to scavenge reactive oxygen species(ROS).Results Results revealed that treatment with inhibitors of abscisic acid(ABA)and γ-aminobutyric acid(GABA)biosynthesis reduced the positive effects of ***,MC-priming increased the contents of ABA and GABA under salt stress by stimulating the expression levels of GhNCED2 and GhGAD4 and the activity of calmodulin-binding(CML)glutamate decarboxylase(GAD).Further analysis showed that an inhibitor of ABA synthesis reduced the positive impacts of MC-priming on the content of GABA under salt stress,but the content of ABA was not affected by the GABA synthesis ***,a multi-omics analysis revealed that MC-priming increased the abundance and phosphorylation levels of the proteins related to ABA signaling,CML,and Ca^(2+)channels/transporters in the MC-primed treatments,which resulted in increased oscillations in Ca^(2+)in the MC-primed cotton seeds under salt *** In summary,these results demonstrate that MC-mediated ABA signaling operates upstream of the GABA synthesis generated by GAD by activating the oscillations of Ca^(2+)and then enhancing activity of the AsA-GSH cycle,which ensures that cotton seeds are tolerant to salt stress.

关键词： seed priming Salinity stress ABA signaling γ-aminobutyric acid ROS scavenging Mepiquat chloride

Different Approaches to Reduce Salinity in Salt-Affected Soils and Enhancing Salt Stress Tolerance in Plants

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Agricultural Sciences 2024年第8期15卷 830-847页

作者： Amra Bratovcic Department of Physical Chemistry and Electrochemistry Faculty of Technology University of Tuzla Tuzla Bosnia and Herzegovina

Salt stress is one of the most harmful environmental stresses in recent times and represents a significant threat to food security. Soil salinization is caused by spontaneous natural processes of mineral dissolution and human activities such as inappropriate irrigation practices. Natural geological progressions like weathering of rocks, arid climate, and higher evaporation, as well as anthropogenic activities, including the use of brackish water for irrigation, and poor tillage operations, are the foremost causes of soil salinization. Typical characteristics of saline soils are salt stress, high pH, and lack of organic carbon, as well as low availability of nutrients. Disruption of precipitation patterns as well as high average annual temperatures due to climate change additionally negatively affects the process of soil salinization. Productivity and ability to support crop growth are reduced on saline soil. Salinity-induced stress reduces plant growth by modulating the antioxidative system and nutrient orchestration. The aim of this work is to show that the mentioned problems can be alleviated in several ways such as the addition of biochar, exogenous application of several elicitors, seed priming, etc. Research has shown that the addition of biochar can significantly improve the recovery of saline soil. The addition of biochar has no significant effect on soil pH, while the cation exchange capacity of the soil increased by 17%, and the electrical conductivity of the saturated paste extract decreased by 13.2% (depends on the initial salinity and the type of biochar raw material). Moreover, biochar enriched with silicon increases the resistance of bananas to salt stress. In addition, exogenous application of several elicitors helps plants to alleviate stress by inducing stress-related physicochemical and molecular changes (selenium, sulfur, silicon, salicylic acid). Finally, seed priming showed positive effects on metabolomics, proteomics and growth of plants subjec

关键词： Salt-Affected Soils Salt Stress Biochar Elicitors seed priming

The Effect of priming on seed Performance of Chickpea （Cicer arietinum L.） under Drought Stress

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Journal of Agricultural Science and Technology(A) 2011年第8期1卷 1349-1351页

作者： A.R. Ajirloo G. R. Mohammadi M. Ghobadi Department of Agronomy and Plant Breeding Campus of Agriculture and Natural Resources Razi University in KermanshahKermanshah *** Iran

In order to investigate the effect of priming on Chickpea seed performance under drought stress, a study was carried out at the seed Research Laboratory of Razi University, Kermanshah, Iran. The experiment was a factorial with three factors arranged in a completely randomized design with four replications. The first factor was seed priming （control （untreated）, HB-101 （Completely organic herbal extracts, derived from the bark of dark cones）, KNO3 and distilled water）, the second was drought stress levels （0.0, -0.3, -0.6, -0.9, -1.2 MPa） and the third was chickpea cultivars （Arman and Azar）. Results indicated that for both cultivars germination percentage （GP）, mean germination rate （MGR）, radicle length （RL）, plumule length （PL） and seedling dry weight （SDW） were reduced when drought stress level were increased from 0 to -!.2 Mpa, although, the cultivars showed different responses to the increased drought stress level. For all of the traits under study, the best results due to the priming treatments were obtained at the drought stress levels higher than -0.6 or -0.9 MPa. So that, at the -1.2 MPa of drought stress level, GP, MGR, and SDW were increased 53.75, 0.49, and 13.85, respectively by HB -101 when compared with control. However, seeds primed with HB-101 showed better performance than those primed with distilled water and KNO3. In general, this study revealed that seed priming especially with HB-101 improved chickpea seed performance under drought stress condition. However, the improvements were more obvious at the higher levels of drought stress.

关键词： Chickpea drought stress seed priming