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Temporal changes of symbiont density and host fitness after rifampicin treatment in a whitefly of the Bemisia tabaci species complex

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Insect Science 2016年第2期23卷 200-214页

作者： Hong-Wei Shan Chang-Rong Zhang Ting-Ting Yan Hai-Qin Tang Xiao-Wei Wang Shu-Sheng Liu Yin-Quan Liu Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou China

Microbial symbionts are essential or important partners to phloem-feeding insects. Antibiotics have been used to selectively eliminate symbionts from their host insects and establish host lines with or without certain symbionts for investigating functions of the symbionts. In this study, using the antibiotic rifampicin we attempted to selectively eliminate certain symbionts from a population of the Middle East-Asia Minor 1 whitefly of the Bemisia tabaci species complex, which harbors the primary symbiont ＂Candidatus Portiera aleyrodidarum＂ and two secondary symbionts ＂Candidatus Hamiltonella defensa＂ and Rickettsia. Neither the primary nor the secondary symbionts were completely depleted in the adults （F0） that fed for 48 h on a diet treated with rifampicin at concentrations of 1 100/zg/mL. However, both the primary and secondary symbionts were nearly completely depleted in the offspring （F 1） of the rifampicin-treated adults. Although the F1 adults produced some eggs （F2）, most of the eggs failed to hatch and none of them reached the second instar, and consequently the rifampicin-treated whitefly colony vanished at the F2 generation. Interestingly, quantitative polymerase chain reaction assays showed that in the rifampicin-treated whiteflies, the density of the primary symbiont was reduced at an obviously slower pace than the secondary symbionts. Mating experiments between rifampicin-treated and untreated adults demonstrated that the negative effects ofrifampicin on host fitness were expressed when the females were treated by the antibiotic, and whether males were treated or not by the antibiotic had little contribution to the negative effects. These observations indicate that with this whitefly population it is not feasible to selectively eliminate the secondary symbionts using rifampicin without affecting the primary symbiont and establish host lines for experimental studies. However, the extinction of the whitefly colony at the second generation after rifampicin

关键词： antibiotic primary symbiont secondary symbiont selective elimination ofsymbiont whitefly

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Phylogeny of the symbionts of tsetse and the evolutionary relationships with their hosts

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Progress in Natural Science:Materials International 1999年第12期 43-49页

作者：陈小爱,李昌本,赵寿元,AKSOY Serap Institute of Genetics State Key laboratory of Genetic Engineering Fudan University Shanghai 200433 China Institute of Genetics State Key laboratory of Genetic Engineering Fudan University Shanghai 200433 China Institute of Genetics State Key laboratory of Genetic Engineering Fudan University Shanghai 200433 China Department of Epidemiology and Public Health Yale University New Haven CT 06520 USA

The phytogeny of the primary-symbionts of eight species belonging to the three subgenera of Glossina was studied based on their 16S rDNA sequences. The results show that these organisms constitute a distinct lineage within the gamma-subdivision of Proteobacteria and have evolved concordantly with their insect host species, suggesting an evolutionarily ancient association for this symbiosis. Based on their almost identical 16S rDNA sequences, the S-en-dosymbionts from five tsetse species belonging to the three subgenuses should be considered as closely related microorganisms . They are the members of the family Enterobacteriaceae within 7-3 subdivision of the Proteobacteria, closely related to enteric bacteria. This high similarity among the S-endosymbionts from different hosts provides strong evidence that the symbiosis acquisition is developed independently by S-endosymbionts of individual tsetse species.

关键词： Glossina primary symbiont secondary symbiont phylogeny 16S rDNA.

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