检索结果-南通市图书馆

Food Science and Human Wellness 2019年第3期8卷 283-291页

作者： Wei Li Shugang Li Yong Hu Mengzhou Zhou Chao Wang Dongsheng Li Deyuan Li Hubei University of Technology Wuhan430068China Hubei Cooperative Innovation Center for Industrial Fermentation Wuhan430068China Function Food Engineering Center of Hubei province Hubei University of Chinese MedicineWuhan430068China

This study aimed to modify isolated and extracted peanut protein with hot alkali to study the impact of pH,heating temperature,processing time and other alkali liquor conditions on the molecular structure of the peanut.Curcumin was loaded in modified peanut protein.The results of the study are as follows:Within the alkaline range of 8

关键词： Curcumin Embedding rate Hot alkali modification Peanut protein secondary structure

Study on the secondary structure and hydration effect of human serum albumin under acidic pH and ethanol perturbation with IR/NIR spectroscopy

在线全文

学校读者我要写书评

暂无评论

创新光学健康科学杂志(英文) 2023年第4期16卷 90-104页

作者： Hui Zhang Mengying Liang Shuangshuang Li Mengyin Tian Xiaoying Wei Bing Zhao Haowei Wang Qin Dong Hengchang Zang NMPA Key Laboratory for Technology Research and Evaluation of Drug Products School of Pharmaceutical Sciences Cheeloo College of Medicine Shandong University Wenhuaxi Road 44 JinanShandong 250012P.R.China National Glycoengineering Research Center Shandong University JinanShandong 250012P.R.China

Human serum albumin(HSA)is the most abundant protein in plasma and plays an essential physiological role in the human body.Ethanol precipitation is the most widely used way to obtain HSA,and pH and ethanol are crucial factors affecting the process.In this study,infrared(IR)spectroscopy and near-infrared(NIR)spectroscopy in combination with chemometrics were used to investigate the changes in the secondary structure and hydration of HSA at acidic pH(5.6-3.2)and isoelectric pH when ethanol concentration was varied from 0%to 40%as a perturbation.IR spectroscopy combined with the two-dimensional correlation spectroscopy(2DCOS)analysis for acid pH system proved that the secondary structure of HSA changed significantly when pH was around 4.5.What's more,the IR spectroscopy and 2DCOS analysis showed different secondary structure forms under different ethanol concentrations at the isoelectric pH.For the hydration effect analysis,NIR spectroscopy combined with the McCabe-Fisher method and aquaphotomics showed that the free hydrogen-bonded water fluctuates dynamically,with ethanol at 0-20%enhancing the hydrogen-bonded water clusters,while weak hydrogen-bonded water clusters were formed when the ethanol concentration increased continuously from 20%to 30%.These measurements provide new insights into the structural changes and changes in the hydration behavior of HSA,revealing the dynamic process of protein purification,and providing a theoretical basis for the selection of HSA alcoholic precipitation process parameters,as well as for further studies of complex biological systems.

关键词： Human serum albumin hydration formation secondary structure IR spectroscopy NIR spectroscopy

Simulating the Folding Pathway of RNA secondary structure Using the Modified Ant Colony Algorithm

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2010年第4期7卷 382-389页

作者： Jun Yu~(1,2),Changhai Zhang~(1,2),Yuanning Liu~(1,2),Xin Li~(1,2) 1.College of Computer Science and Technology,Jilin University,Changchun 130012,P.R.China 2.Key Laboratory of Symbolic Computation and Knowledge Engineering (Ministry of Education,China) Jilin University,Changchun 130012,P.R.China College of Computer Science and Technology Jilin University Changchun P. R. China Key Laboratory of Symbolic Computation and Knowledge Engineering (Ministry of Education China) Jilin University Changchun P. R. China

A new method for simulating the folding pathway of RNA secondary structure using the modified ant colony algorithmis proposed.For a given RNA sequence,the set of all possible stems is obtained and the energy of each stem iscalculated and stored at the initial stage.Furthermore,a more realistic formula is used to compute the energy ofmulti-branch loop in the following iteration.Then a folding pathway is simulated,including such processes as constructionof the heuristic information,the rule of initializing the pheromone,the mechanism of choosing the initial andnext stem and the strategy of updating the pheromone between two different stems.Finally by testing RNA sequences withknown secondary structures from the public databases,we analyze the experimental data to select appropriate values forparameters.The measure indexes show that our procedure is more consistent with phylogenetically proven structures thansoftware RNAstructure sometimes and more effective than the standard Genetic Algorithm.

关键词： RNA secondary structure folding pathway ant colony algorithm

Morphology,Bending Property and secondary structure Estimation of Dog Hair

在线全文

学校读者我要写书评

暂无评论

Journal of Donghua University(English Edition) 2016年第1期33卷 66-71页

作者：王小丽张莉莎刘洪玲杜赵群于伟东 Textile Materials and Technology Laboratory Donghua University

The morphology, bending property and secondary structure estimation of dog hair were investigated by scanning electron microscope( SEM),fiber compression bending analyzer,fiber frictional coefficient tester and Fourier transform infrared spectroscopy( FTIR). The SEM micrograph of hair indicated guard hair( GH),intermediate hair( IH) and underhair( UH) from dog hair fibers displayed considerable differences in the diameter,length,scale shape and medulla. In addition,the bending property of fibers were related to the diameter of fibers and the percentage and structure of medulla. The UH had the greatest frictional coefficient,while the guard hair had the largest bending rigidity in three kinds of hairs. The analysis of amide I region implied that there was an apparent variety in the secondary structure of hairs,mainly the percentage of α-helix and β-pleated sheet and β-turn structure. The X-ray diffraction results showed that the crystallinity of the UH was the lowest in the three kinds of fibers. The tensile behaviors of dog hair also indicated that the increase of β-pleated and β-turn structure caused the increase of the breaking strength.

关键词： dog hair secondary structure morphology bending rigidity frictional property

Sequence-specific Assignment of ~1H-NMR Resonance and Determination of the secondary structure of Jingzhaotoxin-I

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第8期37卷 567-572页

作者： Xiong-Zhi ZENG Qi ZHU Song-Ping LIANG~* College of Life Sciences,Hunan Normal University,Changsha 410081,China College of Life Sciences Hunan Normal University Changsha 410081 China

Jingzhaotoxin-I（JZTX-I）purified from the venom of the spider Chilobrachys jingzhao is anovel neurotoxin preferentially inhibiting cardiac sodium channel inactivation by binding to receptor site 3.The structure of this toxin in aqueous solution was investigated using 2-D 1H-NMR techniques.The completesequence-specific assignments of proton resonance in the 1H-NMR spectra of JZTX-I were obtained byanalyzing a series of 2-D spectra,including DQF-COSY,TOCSY and NOESY spectra,in H2O and D2O.Allthe backbone protons except for Gln4 and more than 95% of the side-chain protons were identified by dαN,dαδ.dβN and dNN connectivities in the NOESY spectrum.These studies provide a basis for the furtherdetermination of the solution conformation of JZTX-Ⅰ.Furthermore,the secondary structure of JZTX-Ⅰwas identified from NMR data.It consists mainly of a short triple-stranded antiparallel [3-sheet with Trp7-Cys9,Phe20-Lys23 and Leu28-Trp31.The characteristics of the secondary structure of JZTX-Ⅰ are similarto those of huwentoxin-Ⅰ（HWTX-Ⅰ）and hainantoxin-Ⅳ（HNTX-IV）,whose structures in solution havepreviously been reported.

关键词： Jingzhaotoxin-I(JZTX-I) 2-D nuclear magnetic resonance(NMR) sequence-specific assignment secondary structure

EFFECTS OF secondary structure ON ELASTIC BEHAVIOR OF PROTEIN-LIKE CHAINS

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2009年第2期27卷 253-265页

作者：孙婷婷 College of Information and Electronic Engineering Zhejiang Gongshang University

The elastic behavior of protein-like chains was investigated by using the Pruned-Enriched-Rosenbluth Method （PERM）.Three typical protein-like chains such as all-α,all-β,andα+β（αβ） proteins were studied in our modified orientation dependent monomer-monomer interaction （ODI） model.We calculated the ratio of〈R;〉/N and shape factor〈δ;〉of protein-like chains in the process of elongation.In the meantime,we discussed the average energy per bond〈U〉/N, average contact energy per bond〈U〉;/N,average helical energy per bond〈U〉;/N and average sheet energy per bond〈U〉;/N.Three maps of contact formation,α-helix formation,β-sheet formation were depicted.All the results educe a view that the helix structure is the most stable structure,while the other two structures are easy to be destroyed.Besides,the average Helmholtz free energy per bond〈A〉/Nis was presented.The force f obtained from the free energy was also discussed.It was shown that the chain extended itself spontaneously first.The force was studied in the process of elongation. Lastly,the energy contribution to elastic force f;was calculated too.It was noted that fu for all-fl chains increased first,and then decreased with x;increasing.

关键词： Protein-like chain Elastic behavior secondary structure PERM.

Novel secondary structure of Calcitonin in Solid State as Revealed by Circular Dichroism Spectroscopy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2002年第7期20卷 697-698页

作者：杜海宁丁金国崔大敷胡红雨 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The solid-state circular dichroic study reveals that salmon calci-toninpresents a typical α-helical structure while human calci-tonin appears to form a β-sheet in solidstate, although both of them adopt random coil ... 详细信息

关键词： calcitonin circular dichroism solid state secondary structure

Minimal secondary structure Formation on mRNAs with a Shine-Dalgarno Sequence for Chromosomal Genes in <i>Rhodobacter sphaeroides</i>

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2021年第10期11卷 529-540页

作者： Damilola Omotajo Hyuk Cho Madhusudan Choudhary Department of Biological Sciences Sam Houston State University Huntsville Texas USA Cumming School of Medicine University of Calgary Calgary Alberta Canada Department of Computer Science Sam Houston State University Huntsville Texas USA

The Shine-Dalgarno (SD) sequence, when present, is known to promote translation initiation in a bacterial cell. However, the thermodynamic stability of the messenger RNA (mRNA) through its secondary structures has an inhibitory effect on the efficiency of translation. This poses the question of whether bacterial mRNAs with SD have low secondary structure formation or not. About 3500 protein-coding genes in Rhodobacter sphaeroides were analyzed and a sliding window analysis of the last 100 nucleotides of the 5’ UTR and the first 100 nucleotides of ORFs was performed using RNAfold, a software for RNA secondary structure analysis. It was shown that mRNAs with SD are less stable than those without SD for genes located on the primary chromosome, but not for the plasmid encoded genes. Furthermore, mRNA stability is similar for genes within each chromosome except those encoded by the accessory chromosome (second chromosome). Results highlight the possible contribution of other factors like replicon-specific nucleotide composition (GC content), codon bias, and protein stability in determining the efficiency of translation initiation in both SD-dependent and SD-independent translation systems.

关键词： Shine-Dalgarno Sequence secondary structure Messenger RNA Translation Initiation

Identification of secondary structure of Extracellular Signal Regulated Kinase (ERK) Interacting Proteins and Their Domain: An in Silico Study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Neuroscience 2021年第1期11卷 67-89页

作者： Kurrey Khuleshwari Paramanik Vijay Cellular and Molecular Neurobiology & Drug Targeting Laboratory Department of Zoology Indira Gandhi National Tribal University Amarkantak (MP) (MP)-484 887 India

ERK is involved in multiple cell signaling pathways through its interacting proteins. By in silico analysis, earlier we have identified 22 putative ERK interacting proteins namely;ephrin type-B receptor 2 isoform 2 precursor (EPHB2), mitogen-activated protein kinase 1 (MAPK1), interleukin-17 receptor D precursor (IL17RD), WD repeat domain containing 83 (WDR83), tescalcin (Tesc), mitogen-activated protein kinase kinase kinase 4 (MAPP3K4), kinase suppressor of Ras2 (KSR2), mitogen-activated protein kinase kinase 6 (MAP3K6), UL16 binding protein 2 (ULBP2), UL16 binding protein 1 (ULBP1), dual specificity phosphatase 14 (DUSP14), dual specificity phosphatase 6 (DUSP6), hyaluronan-mediated motility receptor (RHAMM), kinase D interacting substrate of 220 kDa (KININS220), membrane-associated guanylate kinase (MAGI3), phosphoprotein enriched in astrocytes 15 (PEA15), typtophenyl-tRNA synthetase, cytoplasmic (WARS), dual specificity phosphatase 9 (DUSP9), mitogen-activated protein kinase kinase kinase 1 (MAP3K1), UL16 binding protein 3 (ULBP3), SLAM family member 7 isoform a precursor (SLAMMF7) and mitogen activated protein kinase kinase kinase 11 (MAP3K11) ( ERK secondary structure Motif Scan Random Coils Alpha Helix Protein Kinases

Shared-probe system:An accurate,low-cost and general enzyme-assisted DNA probe system for detection of genetic mutation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第6期33卷 3043-3048页

作者： Lida Ren Zhihao Ming Wei Zhang Yangwei Liao Xiaofeng Tang Bei Yan Huimin Lv Xianjin Xiao Centre of Reproductive Medicine/Family Planning Research Institute Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Obstetrics and Gynecology Third Hospital of Shanxi Medical University.Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalTongji Medical College.Huazhong University of Science and TechnologyTaiyuan 030032China

Enzyme assisted DNA probes are powerful tools in molecular diagnostics for their simplicity,rapidity,and low detection limit.However,cost of probes,difficulty in optimization and disturbance of secondary structure hindered the wider application of enzyme assisted DNA probes.To solve the problems,we designed a new system named shared-probe system.By introducing two unlabeled single stranded DNA named Sh1 and Sh2 as the bridge between probe and the substrate,the same sequence of dually labeled probe with stable performance was shared for different mutations,thus sparing the expense and time cost on designing,synthesizing and optimizing corresponding probes.Besides,the hybridization between Sh1 and the substrate could overcome secondary structures,which guaranteed the detection of different substrates.The performance and generality of the design were tested by low abundance detection in synthetic single DNA samples and the limit of detection was 0.05%for PTENR130 Q,EGFR-L858 R and 0.02%for BRCA1-NM007294.3.In genomic DNA samples,the limit of detection of 0.1%can be achieved for EGFR-L858 R,demonstrating the potential of clinical application in our design.

关键词： Nucleic acid probe EndonucleaseⅣ secondary structure Genomic mutation SNP detection