检索结果-南通市图书馆

Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

引用

Stress Biology 2022年第1期2卷 299-311页

作者： Mirza Abid Mehmood Yanping Fu Huizhang Zhao Jiasen Cheng Jiatao Xie Daohong Jiang State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China Plant Pathology Institute of Plant ProtectionMNS University of AgricultureMultan 60000Pakistan Provincial Key Laboratory of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityHubei ProvinceWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China

Rice sheath blight pathogen,Rhizoctonia solani,produces numerous sclerotia to *** a rich source of nutrients in the soil,sclerotia may lead to the change of soil *** this purpose,we amended the sclerotia of *** in soil and analyzed the changes in bacterial microbiota within the soil at different time *** the phyla level,Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the *** increased abundance of ammonia-oxidizing bacterium(AOB)Nitrosospira and Nitrite oxidizing bacteria(NOB)i.e.,Nitrospira was observed,where the latter is reportedly involved in the nitrifier ***,Thiobacillus,Gemmatimonas,Anaeromyxobacter and Geobacter,the vital players in denitrification,N2O reduction and reductive nitrogen transformation,respectively,depicted enhanced abundance in *** sclerotia-amended ***,asymbiotic nitrogen-fixing bacteria,notably,Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the *** growth promoting bacteria,such as Kribbella,Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended *** per our knowledge,this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of *** sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

关键词： Rice sheath blight Rhizoctonia solani sclerotia Reductive nitrogen transformation Nitrogen fixation Soil microbiome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

sclerotia Formation and Toxin Production in Large sclerotial Aspergillus flavus Isolates from Kenya

引用

Advances in Microbiology 2016年第1期6卷 47-56页

作者： S. A. Okoth B. Nyongesa V. Joutsjoki H. Korhonen V. Ayugi E. K. Kang’ethe School of Biological Sciences University of Nairobi Nairobi Kenya Natural Resources Institute Finland Jokioinen Finland Department of Public Health Pharmacology and Toxicology University of Nairobi Nairobi Kenya

We studied the relationship between sclerotia formation and aflatoxin production by Aspergillusflavus strains isolated from maize kernels from Nandi County. Isolates recovered from maize kernels were tested for their ability to form sclerotia on different growth media. PCR analysis was done on the isolates to detect 2 structural genes, aflD and aflQ, involved in aflatoxin biosynthesis pathway. Positive A. flavus isolates for one or both genes were grown on Yeast Extract Sucrose Agar medium and aflatoxins quantified using LCMSMS. All the isolates formed large sclerotia and their formation was influenced by media type but could not be related to amount of aflatoxins produced both in vivo and in vitro. Though sclerotia are perennating structures and so contribute to survival index of a fungus, their initiation is regulated by external factors though ability to form is genetic. This brings ambiguity of their presence or abundance as a measure of toxicity.

关键词： sclerotia Aflatoxins Maize Kenya Aspergillus flavus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcription factor SsSte12 is involved in sclerotia development and appressoria production in Sclerotinia sclerotiorum

Transcription factor SsSte12 is involved in Sclerotia develo...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Lu Dongxu Liu Fengwen Sun Rongze Wang Kangning Zhang Bowen Zhang Yanhua Pan Hongyu College of Plant Sciences Jilin University

Sclerotinia sclerotiorum is a challenging agricultural pathogen for management,causing large global economic losses *** sclerotia and infection cushions are critical for its long-term survival and successful penetration on a wide spectrum of *** mitogen-activated protein kinase(MAPK) cascades serve as central signaling complexes that are involved in various aspects of sclerotia development and *** this study,the putative downstream transcription factor of MAPK pathway,SsSte12,was analyzed in *** SsSte12 in *** resulted in phenotypes of delayed vegetative growth,reduced size of sclerotia,and fewer appressoria ***,the SsSte12 RNAi mutants showed attenuated pathogenicity on the host plants due to the defect compound *** two-hybrid(Y2H) and bimolecular fluorescence complementation(BiFC) assays demonstrated that the SsSte12 interacts with ***,the SsMcm1 expression is independent of the regulation of SsSte12 as revealed by qRT-PCR analysis in SsSte12 RNAi *** with high accumulation of SsSte12 transcripts in the early development of *** results demonstrated that SsSte12 function was essential in the vegetative mycelial growth,sclerotia development,appressoria formation and penetration-dependent ***,the SsSte12-SsMcm1 interaction might play a critical role in the regulation of the genes encoding these traits in ***.

关键词： SsSte12 sclerotia Appressoria Pathogenicity SsMCM1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Nutrient Value of Protein from Pleurotus tuber-regium sclerotia Grown with Different Nitrogen Sources

引用

食用菌学报 2006年第3期 52-54页

作者： LEI Jin-gui~ 1* , WENG Bo-qi~2, JIANG Zhi-he~1, TANG Xiang-qiu~1, WANG Yi-xiang~2 ~1 The Development and Application Research Center of Edible Fungi,Fujian Academy of Agricultural Sciences, Fuzhou 350013, China ~2 Institute of Agricultural Zoology Project, Academy of Agricultural Sciences, Fuzhou 350013, China

Protein from P. tuber-regium sclerotia cultivated using four different nitrogen sources [urea, cattle dung, （NH4）2SO4and wheat bran] was assessed in terms of six nutritive quality indices—Amino Acid Score （AAS）, Chemical Score （CS）,Essential Amino Acid Index （EAAI）,Biological Value （BV）, Nutritional Index （NI） and Amino Acid Ratio Coefficient Score （AARCS）. Protein from sclerotia cultivated with urea as the nitrogen source had a superior overall nutrient value and was ranked highest in terms of all six indices. Protein from sclerotia grown with wheat bran was ranked second in the case of three protein quality indices （AAS, CS and NI） and third for the remaining three indices （EAAI, BV and AARCS）. Protein from sclerotia grown with （NH4）2SO4was ranked second in terms of three indices （EAAI,BV and AARCS） and third for the remaining three indices. Protein from sclerotia grown with cattle dung had the lowest nutrient value and was ranked lowest in terms of all six quality indices.

关键词： Nitrogen source Pleurotus tuber-regium sclerotia Protein quality Nutritive value

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Enrichment of beneficial and suppression of pathogenic microorganisms in response to the addition of Sclerotinia sclerotiorum sclerotia in soil and its effect on microbial diversity

Enrichment of beneficial and suppression of pathogenic micro...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Mirza Abid Mehmood Xie Jiatao Cheng Jiasen Fu Yanping Jiang Daohong The State Key Laboratory of Agriculture Microbiology Huazhong Agricultural University The Key Laboratory of Plant Pathology of Hubei Province Huazhong Agricultural University

Soil microbiome plays an imperative role due to their multifunctional activities including suppression of soil-borne *** sclerotiorum is a notorious soil-borne pathogen of various important crops and produce sclerotia to survive within soil for long *** is enriched in carbohydrates,fats,proteins and chitin which can be utilized by microbes to obtain nutrients required for their optimum *** in view the sclerotia as a nutritional source for microbes,we hypothesized that sclerotia in soil may affect the microbial diversity directly or/and *** this study,we incubated sclerotia of *** in soil collected from field to observe shift-in microbial diversity across three months using 16S rRNA and ITS sequencing *** observed surprising shift-in microbial diversity with non-amended soil having more diverse microbes than sclerotia-amended soil *** diversity indices confirmed the decline in diversity of sclerotia-amended soil samples of bacterial and fungal communities with the exception of 3 month in fungal community where more diverse community was observed in sclerotia-amended soil samples compared to the non-amended ***-amended soil activated the antagonists and plant growth promoting microbes whereas,several plant-pathogenic fungi depicted decline in their abundances with the passage of ***,bacteria with potential role in plant growth promotion showed enhanced abundance in sclerotia-amended soils,such as Burkholderia,Chitinophaga and Dyella ***,fungal diversity exhibited enrichment of fungi with reported antagonistic activities such as,Clonostachys,Trichoderma and Talaromyces *** the contrary,we observed a drastic decline in abundance of numerous notorious plant-pathogenic fungi such as,Alternaria alternata,Botrytis cinerea,Fusarium graminearum,***,***,*** and Sclerotiumrolfsii *** sclerotia-amended soil ***,we conclude that

关键词： Enrichment Suppression Microbiome Sclerotinia sclerotiorum sclerotia 16S rRNA ITS

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Anti-inflammatory,Anti-oxidant and Xanthine oxidase Inhibitory Activities of Polyporus umbellatus sclerotia

Anti-inflammatory,Anti-oxidant and Xanthine oxidase Inhibito...

引用

2013首届全国猪苓会议

作者： Hak Hyun Ko Yong Mei Xing Shun Xing Guo Kyung Hoan Im Min Woong Lee Tae Soo Lee Division of Life Sciences University of IncheonIncheon406-840Korea Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingPeople's Republic of China. Department of Life Sciences Dongguk UniversitySeoul 100-715Korea

＜正＞Polyporus umbellatus（*** umbellata） is an edible and medicinal mushroom,belongs to the Polyporales of *** study was initiated to evaluate the anti-oxidant,anti-inflammatory and anti-xanthine oxidase activities of *** sclerotia extracted with acetone,methanol and hot *** antioxidant activities were performed on reducing power,1,l-diphenyl-2-picrylhydrazyl free radical scavenging, and ferrous chelating *** addition to this,polyphenol and flavonoid contents were also *** 8 mg/ml,hot water extract showed the highest reducing power of *** scavenging activities of *** on 1,1-diphenyl-2-picrylhydrazyl radicals,acetone extract was more effective than methanol and hot water *** strongest chelating effect（67.50%） was obtained from the methanol extract at 1.0 mg/ml *** antioxidant activities of the extracts from sclerotia of *** were increased with the increasing *** xanthine oxidase inhibitory activities of 3 different extracts of the sclerotia were not good as allopurinol,a positive control,and the inhibitory activities were increased with the increasing concentration of the *** anti-inflammatory activity of hot water extract of the sclerotia was measured on carrageenan-induced hind paw edema of albino rat and the mean increase in paw volume and%inhibition in paw volume was measured by plethysmometer at different time intervals after different concentrations of carrageenan were *** injection of hot water extract with 20 mg/kg showed significant reduction in the carrageenan-induced paw edema compared with the *** highest concentration of polyphenol was found in hot water,whereas acetone extract showed the highest concentration of *** experimental results showed that acetone and methanol extract showed good,while hot water extract showed moderate xanthine oxidase inhibitory activities at the concentration *** invest

关键词： Anti-inflammation Anti-oxidant Anti-xanthine oxidase Polyporus umbellatus sclerotia

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Function on the transcriptional factor SsFKH1 to sclerotia and cushion development in Sclerotinia sclerotiorum

Function on the transcriptional factor SsFKH1 to sclerotia a...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Cong Jie Liu Jinliang Zhang Yanhua Zhang Xianghui Pan Hongyu College of Plant Sciences Jilin University

Sclerotinia sclerotiorum(Lib.) de Bary is a necrotrophic plant pathogenetic fungus with broad host range and worldwide *** sclerotia and cushion of *** will be induced and produced into pigmented multicellular structures from the aggregation of hyphae during the vegetative development stages respectively under ambient pH,cAMP and light *** are requried for the production of fruiting *** the cushion play a central role in the life and infection cycles of this *** the basis of some research previously,Ss-Fkh1,forkhead(FKH)-box-containing protein,is an important transcriptional factor involved information and development of sclerotia and *** investigate the role of Ss-Fkh\ in ***,the partial sequence of Ss-Fkh1 was cloned and RNA interference(RNAi)-based gene silencing was employed to alter the expression of *** mutants with significantly reduced Ss-Fkhl RNA levels exhibited slow-growing hypha and developmental defects *** addition,the expression levels of a set of putative melanin biosynthesis-related laccase genes and a polyketidesynthase-encoding gene were significantly down-regulated insilenced *** test results demonstrated that RNAi-silenced strains was significantly compromised with the development of a smaller infection lesion on tomato ***,the resulting mutant displays series phenotypes that mycelial growth rate decreased significantly when compared Ss-Fkh1 knockout mutant with the wild-type,it’s development stage remained in S1 stage of ***,the results suggest that Ss-Fkh1 is involved in hyphalgrowth,virulence and sclerotial formation in ***.

关键词： Sclerotinia sclerotiorum Transcriptional factor SsFKH1 sclerotia Cushion

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Extracellular Enzyme Activity of sclerotia-forming and Non-sclerotia-forming Morchella Strains

引用

Agricultural Science & Technology 2013年第10期14卷 1392-1396,1408页

作者：陈立佼王芳赵永昌黄兴奇柴红梅云南大学生命科学学院云南昆明650091 云南省农业科学院生物技术与种质资源研究所云南昆明650223 云南省农业科学院教育培训处云南昆明650205

The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica exhibited higher aver- age activities of protease, catalase and xylanase but lower laccase activity than those of sclerotia-forming strains. However, the differences did not reach significance level. The soluble amylases activity of M. crasspies was significantly higher than that of M. conica, while the mean activity of other enzymes had differences between them, but the differences did not reach significance level.

关键词： Morchella conica Morchella crasspies sclerotia Extracellular enzyme

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Discovery and Application of the Companion Fungus Related to sclerotial Formation from Hyphae of Grifola umbellata

引用

Acta Botanica Sinica 2002年第10期44卷 1151-1154页

作者：郭顺星王秋颖庄文颖张集慧邢晓科中国医学科学院中国协和医科大学药用植物研究所北京100094 中国科学院微生物研究所北京100080

The companion fungus ( Grifola sp.) related to sclerotial formation from hyphae of Grifola umbellata (Pers.) Pilat was isolated from the cavity associated with sclerotia of G. umbellata in natural condition. Experimental results showed that the pure culture of G. umbellata was unable to form sclerotia, whereas sclerotia produced easily in flasks or on trunks when the companion fungus was inoculated together with strain of G. umbellata. The companion fungus is critical for sclerotium formation from hyphae of G. umbellata. Morphological differences were found in cultures of the two fungi. The companion fungus possesses thin-walled narrow hyphae, while G. umbellata is of wider hyphae which are either thick- or thin-walled.

关键词： Grifola umbellata companion fungus sclerotia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gene Expressing Difference in sclerotial Formation of Morchella conica

Gene Expressing Difference in Sclerotial Formation of Morche...

引用

2017第二届全国羊肚菌大会暨湖北省食用菌协会第八次会员代表大会

作者： Li-Jiao Chen Hong-Mei Chai Wei-Min Chen Xing-Qi Huang Yong-Chang Zhao

The difference of gene expression betweensclerotia-producing and non-sclerotia producing singlespore isolates from Morchella conica were preliminaryanalyzed by mRNA differential display reverse transcrip-tionpolymerase chain reaction(RT-PCR) technique and 67 differential gene fragments were ***-eight oftheir second PCR products were cloned and *** special differential gene fragments related tosclerotial formation were validated by semiquantitative *** gene fragments had certain homologies with lipoprotein,cyclin-dependent kinase C-3,glycerophosphoryl diester phosphodiesterase,Rho GDPdissociation inhibitor,gamma-aminobutvrate permease,OmpA family protein,Transcript antisense to ribosomal RNA protein,sodiumcalcium exchange protein and keratinassociated proteins 5,*** addition,the putative protein of some DNA fragments had higher similarity with hypothetical proteincoding gene in NCBI database,as well as some were only putative gene *** these fragments were speculated to be the functional gene associated with sclerotial formation in morel.

关键词： mRNA differential display Morchella conica sclerotia Semi-quantitative RT-PCR

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：