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Asian Pacific Journal of Tropical Medicine 2012年第3期5卷 220-224页

作者： Shyamapada Mandal Manisha Deb Mandal Nishith Kumar Pal Department of Microbiology Bacteriology and Serology UnitCalcutta School of Tropical MedicineC.R.AvenueKolkata-700073India Department of Physiology and Biophysics KPC Medical College and HospitalJadavpurKolkata-700032India

Objective:To evaluate the antibacterial activity of Ocimum sanctum(***) leaf extract, alone,and in combination with chloramphenicol(C) and trimethoprim(Tm) against Salmonella enterica serovar Typhi(***).Methods:The antibacterial activity of ethanolic extract of tulsi, ***,leaf(TLE:500μg) for 23 *** isolates was determined following agar diffusion. The C(30μg) and Tm(5μg) activity alone and in combination with TLE(250μg) was determined by disk *** zone diameter of inhibition(ZDI) for the agents was recorded, and growth inhibitory indices(Glls) were ***:The *** isolates(n=23),which were resistant to both C(ZDI 6 mm) and Tm(ZDI 6 mm),had TLE(500μg) ZDIs 16-24 *** ZDIs of C and Tm were increased up to 15-21 mm and 17-23 mm,respectively,when TLE(250μg) was added to the C and Tm *** Glls ranged 0.789-1.235 and 0.894-1.352,due to combined activity against *** isolates,of C and TLE and Tm and ***:The data suggest that TLE,in combination with C and Tm,had synergistic activity for *** isolates, and hence *** is potential in combating *** drug resistance,as well promising in the development of non-antibiotic drug for *** infection.

关键词： Antibacterial activity Ocimum sanctum Chloramphenicol Trimethoprim Growth inhibitory index Synergy Salmonella enterica serovar Typhi

Effect of Ocimum sanctum on the development of protective immunity against Salmonella typhimurium infection through cytokines

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Asian Pacific Journal of Tropical Medicine 2010年第9期3卷 682-686页

作者： Anjana Goel Sandeep Kumar Ashok Kumar Bhatia Department of Microbiology & Immunology College of Veterinary Sciences & Animal HusbandryU.P.Pt Deen Dayal Upadhyay Pasu Chikitsa Vigyan Vishwavidyalaya evam Go Anusandhan Sansthan(DUVASU)Mathura281001U.P.India

Objective:To investigate the protective role of Ocimum sanctum（***） leaves against Salmonella ***（***） infection in rats by inducing TNF-α,IFN-γ& IL-2 ***:Wistar albino rats were fed with aqueous extract of *** leaves using 250 mg/kg body weight dose once a day for 20 consecutive *** rats were fed with *** were infected with LD50 dose of *** infection and monitored for their *** blood burden in both the groups was compared and numbers of activated peritoneal macrophages were *** of TNF-α,IFN-γand IL-2 cytokines in serum during different time intervals was assayed by sandwich ***:Rats of control group showed a high mortality rate and had higher bacterial blood burden when compared with *** extract fed *** was a significant increase in the number of *** engulfed peritoneal macrophages in the peritoneal fluid of *** fed *** protective control against bacterial infection in O,sanctum fed rats was associated with elevated level of TNF-α,IFN-γand IL-2 cytokines in ***:These findings suggest that orally administered *** leaves extract effectively enhanced activation in macrophage and lymphocytes,depicted by the elevated serum concentration of TNF-α,IFN-γand IL-2 cytokines,leading to induce a protective resistance against Salmonella typhimurium infection.

关键词： Ocimum sanctum Salmonella typhimurium TNF-α IFN-γ IL-2

Immunomodulating property of Ocimum sanctum by regulating the IL-2 production and its mRNA expression using rat's splenocytes

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Asian Pacific Journal of Tropical Medicine 2010年第1期3卷 8-12页

作者： Anjana Goel Dilip Kumar Singh Sandeep Kumar Ashok Kumar Bhatia Department of Microbiology & Immunology College of Veterinary Sciences & Animal HusbandryU.P.Pt Deen Dayal Upadhyay Pasu Chikitsa Vigyan Vishwavidyalaya evam Go Anusandhan Sansthan(DUVASU)Mathura281001U.P.India

Objective:To investigate the regulatory effect of aqueous extract of leaves of Ocimum sanctum on IL-2 cytokine production in vivo and in vitro,and the effect of leaves extract on general blood picture including T& B ***:For in vivo studies albino Wistar rats were treated with aqueous crude leaves extract of Ocimum sanctum for 20 consecutive *** cells were harvested and assayed for IL-2 production by using sandwich enzyme-linked immunosorbent assay(ELISA) and mRNA expression *** in vitro study aqueous Ocimum sanctum leaves extract= in different concentrations(25-500μ/mL) was added into culture plates containing ConA stimulated *** study the overall effect on blood picture,density gradient purified lymphocytes analysis and conventional methodology for total and differential leukocyte count and hemoglobin level were also ***:It indicated that the rats treated with Ocimum sanctum leaves extract had significantly enhanced(Psanctum leaves extract may have stimulatory effect on T & B lymphocytes particularly on Th 1 subset of lymphocytes as shown by enhancement in IL-2 production.

关键词： B lymphocytes IL-2 Ocimum sanctum Splenocytes T lymphocytes

Isolation and characterization of anti-diabetic component(bioactivity-guided fractionation) from Ocimum sanctum L.(Lamiaceae) aerial part

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Asian Pacific Journal of Tropical Medicine 2011年第4期4卷 278-282页

作者： Raju Patil Ravindra Patil Bharati Ahirwar Dheeraj Ahirwar CEC School of Pharmacy Lal KhadanMasturi RoadBilaspur(C.G.)India S U College of Pharmaceutical Sciences and Research Department of Pharmacognosy Guru Ghasidas University

Objective:To isolate and characterize antidiabetic component（bioactivity-guided fractionation） from hydro alcoholic extract of Ocimum sanctum（***） aerial ***:Ten fractions（F1 - F10） were isolated from hydro alcoholic extract of *** aerial part by column *** the fractions Fl to F10 were screened for antidiabetic activity in alloxan induced diabetic rats by estimating serum glucose level and lipid *** isolated bioactive component was elucidated on the basis of extensive spectroscopic(UV,IR,MS,1H and 13C NMR) data ***:The bioactive fraction（F5） was found to be potent antidiabetic by ameliorating glucose and lipid parameters（total cholesterol,triglycerides,low and high density lipoprotein cholesterol）.The extensive spectroscopic data analysis reveals that,the isolated bioactive compound elucidated as tetracyclic triterpenoid[16-Hydroxy-4,4,10,13-tetramethyl- 17-（4-methyI-pentyl）-hexadecahydro-cyclopenta[a]phenanthren-3-one].Conclusions:Our present study concluded that,tetracyclic triterpenoid isolated from aerial part of *** has a great anti-diabetic potential.

关键词： Ocimum sanctum Diabetes Bioactivity guided fractionation

Antifungal activity of Ocimum sanctum Linn.(Lamiaceae) on clinically isolated dermatophytic fungi

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Asian Pacific Journal of Tropical Medicine 2011年第8期4卷 654-657页

作者： Balakumar S Rajan S Thirunalasundari T Jeeva S Department of Chemistry and Biosciences SASTRA UniversitySrinivasa Ramanujan Centre.Kumbakonam- 612001Tamil NaduIndia Department of Microbiology M.R.Government Arts CollegeMannargudi.Thriuvarur District.Tamil NaduIndia Department of Biotechnology Bharathidasan UniversityTiruchirappalli-620 024Tamil Nadu.India Centre for Biodiversity and Biotechnology.Department of Botany Nesamony Memorial Christian College.Marthandam-629 165Tamil Nadu.India

Objective:To assess antifungal activity of Ocimum sanctum leaves against dermatophytic fungi. Methods:Antifungal activity of Ocimum sanctum leaves was measured by 38 A NCCLS method. Minimum inhibitory concentration(MIC) and minimum fungicidal concentration(MFC) of various extracts and fractions of Ocimum sanctum leaves were also ***:Ocimum sanctum leaves possessed antifungal activity against clinically isolated dermatophytes at the concentration of 200μg/*** and MFC were high with water fraction(200μg/mL) against dermatophytic fungi ***:Ocimum sanctum has antifungal activity,and the leaf extracts may be a useful source for dermatophytic infections.

关键词： Antifungal activity Dermatophytosis Epidermophyton floccosum Minimum fungicidal concentration Microsporum sp Minimum inhibitory concentration Ocimum sanctum Trichophyton sp

Anti-Oxidative, Anti-Hyperglycemic and Lipid-Lowering Effects of Aqueous Extracts of <i>Ocimum sanctum</i>L. Leaves in Diabetic Rats

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Food and Nutrition Sciences 2014年第9期5卷 801-811页

作者： Thamolwan Suanarunsawat Watcharaporn Devakul Na Ayutthaya Suwan Thirawarapan Somlak Poungshompoo Department of Pathology Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand Department of Physiology Faculty of Pharmacy Mahidol University Bangkok Thailand Pharmacology and Toxicology Unit Department of Medical Sciences Faculty of Science Rangsit University Pathumtani Thailand Physiology Unit Department of Medical Sciences Faculty of Science Rangsit UniversityPathumtaniThailand

The present study was conducted to investigate the effect of aqueous extracts of Ocimum sanctum L. leaves on blood glucose, serum lipid profile and anti-oxidative activity to protect various risk organs in DM rats. Ocimum sanctum L. leaves were extracted using water, then the total phenolic content was determined. Three groups of male Wistar rats were used including normal control rats, DM rats and DM rats daily fed with aqueous extracts of Ocimum sanctum L. leaves (AQOS) for three weeks. DM rats were induced by intraperitoneal injection of streptozotocin (65 mg/kgbw). The results show that three weeks of diabetic induction increased blood glucose, serum lipid profile and serum levels of AST, ALT, ALP, LDH, CK-MB, creatinine and BUN. AQOS significantly decreased blood glucose, serum lipid profile and serum levels of AST, ALT, ALP, LDH, CK-MB, creatinine and BUN. The low level of serum insulin was also raised by AQOS. AQOS suppressed high TBARS level and raised the activities of antioxidant enzymes in the liver, kidney and cardiac tis- sues. Histopathological results show that AQOS preserved the liver, kidney and myocardial tissues. It can be concluded that AQOS had anti-hyperglycemic, anti-hyperlipidemic, and free radical sca- venging effects providing organ protection from diabetes. The phenolic compounds contained in AQOS might be responsible for these activities.

关键词： Ocimum sanctum L. Leaves Diabetic Rats Antioxidant Enzymes Phenolic Compound

Antifungal Activity and Qualitative Phytochemical Analysis of Some Medicinal Plants in Jaffna (Sri Lanka)

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International Journal of Organic Chemistry 2018年第4期8卷 335-340页

作者： Tharmarajah Manoranjan Reeka Thangarajah A. C. Thavaranjit Department of Chemistry University of Jaffna Jaffna Sri Lanka Department of Botany University of Jaffna Jaffna Sri Lanka

Many members of the Labiatae family are used in traditional and folk medicine and also used as culinary and ornamental plants. Leaves are the most used plants parts of this family. Ethanolic extract of the leaves, stem, seeds of Leucas zeylanica, Ocimum canum, Ocimum sanctum and leaves of Mentha arvensis, Ocimum basilicum were subjected to phytochemical screening and antifungal assays against Aspergillus sp., Penicillium sp., Trichoderma sp., Mucor sp., Rhizopus sp. was determined by using the agar streaking assay method after 48 and 72 hours of incubation. All parts of the plants were found to contain flavonoid/s and alkaloid/s except for the absence of alkaloids in seeds of L. zeylanica and stem of O. sanctum respectively. Tannins were present in all parts of plants such as L. zeylanica, O. canum, M. arvensis and absent in O. sanctum and O. basilicum. Phlobatannins were only present in leaves of L. zeylanica and saponins were present only in leaves of O. basilicum. The leaves of L. zeylanica, O. basilicum, M. arvensis, O. sanctum and seeds of O. sanctum and O. canum showed the presence of steroids. Terpenoids were present in all parts of O. sanctum and O. canum than the other plants. The cardiac glycosides were present in all parts of O. sanctum than the other plants tested. Leaves of O. sanctum and M. arvensis exhibited strong positive antifungal activity against Aspergillus sp. Leaves of O. canum, O. basilicum and M. arvensis and stem of O. canum showed strong positive activity against Mucor sp. L. zeylanica only exhibited the antifungal activity against Mucor sp. Penicillium sp. was inhibited by the leaves and seeds extracts of O. sanctum. Degree of activity was low in L. zeylanica compared with other plant extracts. Most of these plant parts did not show any activity against Trichoderma sp. and Rhizopus sp. This study revealed that the antifungal activity of leaves of these plants was high than other plant parts against tested fungi.

关键词： Leucas zeylanica Ocimum canum Ocimum sanctum Mentha arvensis Ocimum basilicum Antifungal Activity

Comparative study on some selected species of <i>Ocimum</i>genus on free radical scavenging activity and hepatoprotective activity against CCl4 induced intoxication in rats

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American Journal of Molecular Biology 2013年第4期3卷 183-186页

作者： Myron Green Rajeswara Rao Pragada Sambasivarao Ethadi Bettaiya Rajanna Alcorn State University Mississippi USA AU College of Pharmaceutical Sciences Andhra University Visakhapatnam India

In this study, we compared the in vitro antioxidant property among the selected Ocimum species (O. sanctum, O. americanum, O. basilicum and O. gratissimum) and hepatoprotective activities of their extracts against CCl4 induced intoxication. The results suggested that the four species of Ocimum genus showed variability in Phenolic content and in vitro antioxi-dant activity against DPPH, superoxide and hydroxyl radicals in the following manner: O. sanctum > O. americanum > O. basilicum > O. gratissimum respec-tively. Based on serum AST, ALT, ALP and T. Bil levels, the alcoholic extracts of Ocimum species showed a significant dose dependent (250 mg and 500 mg and 750 mg/kg p.o.) and a protective effect against CCl4 induced hepatotoxicity in albino rats. The results further revealed that the potential hepatoprotective activity of Ocimum sanctum among the Ocimum species.

关键词： Ocimum sanctum Ocimum americanum Ocimum basilicum Ocimum gratissimum Antioxidant Activity Carbon Tetrachloride Hepatotoxicity