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A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing

Science China Chemistry 2020年第10期63卷 1498-1506页

作者： Fei Tian Chao Liu Jinqi Deng Ziwei Han Lu Zhang Qinghua Chen Jiashu Sun CAS Key Laboratory of Standardization and Measurement of Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Capital Normal UniversityBeijing 100048China

The outbreak of virus-induced infectious diseases poses a global public-health *** acid amplification testing(NAAT)enables early detection of pandemic viruses and plays a vital role in preventing onward ***,the requirement of skilled operators,expensive instrumentation,and biosafety laboratories has hindered the use of NAAT for screening and diagnosis of suspected *** we report development of a fully automated centrifugal microfluidic system with sample-in-answer-out capability for sensitive,specific,and rapid viral nucleic acid *** release of nucleic acids and the subsequent reverse transcription loop-mediated isothermal amplification(RT-LAMP)were integrated into the reaction units of a microfluidic *** whole processing steps such as injection of reagents,fluid actuation by rotation,heating and temperature control,and detection of fluorescence signals were carried out automatically by a customized *** validate the centrifugal microfluidic system using oropharyngeal swab samples spiked with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)armored RNA *** estimated limit of detection for armored RNA particles is 2 copies per reaction,the throughput is 21 reactions per disc,and the assay sample-to-answer time is approximately 70 *** enclosed and automated microfluidic system efficiently avoids viral contamination of aerosol,and can be readily adapted for virus detection outside the diagnostic laboratory.

关键词： nucleic acid testing virus microfluidics sample-to-answer automation

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