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Characterization of a salt-tolerant bacterium bacillus sp. from a membrane bioreactor for saline wastewater treatment

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Journal of Environmental sciences 2014年第6期26卷 1369-1374页

作者： Xiaohui Zhang Jie Gao Fangbo Zhao Yuanyuan Zhao Zhanshuang Li Pathology Department Fourth Clinical Hospital of Harbin Medical University school of Material science and Chemical Engineering Harbin Engineering University

High salt concentrations can cause plasmolysis and loss of activity of cells, but the salt-torlerant bacterium can endure the high salt concentrations in wastewater. In this research 7 salt-torlerant bacteria, which could survive in dry powder products and could degrade organic contaminants in saline wastewater, were isolated from a membrane bioreactor. The strain NY6 which showed the fastest growth rate, best property for organic matter degradation and could survive in dry powder more than 3 months was selected and characterized. It was classified as bacillus aerius based on the analysis of the morphological and physiological properties as well as the 16s rrna sequence and Neigh borjoining tree. The strain NY6 could survive in the salinity up to 6% and the optimal growth salinity is 2%; it belongs to a slightly halophilic bacterium. The capability of its dry powder products for COD removal was 800 mg COD/（***） in synthesized saline wastewater with salinity of 2%. According to salt-tolerant mechanism research, when the salinity was below 2%, the stain NY6 absorbed K~ and Na~ to maintain osmotic equilibrium, and when the salinity was above 2%, the NY6 kept its life by producing a large amount of spores.

关键词： salt-torlerant bacterium saline wastewater bacteria bacillus 16s rrna

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