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Rheological properties of salt-tolerant HPAM solutions with ultrahigh molecular weight

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Journal of Central South University 2008年第S1期15卷 93-97页

作者：张敏革张吕鸿姜斌李鑫钢 School of Chemical Engineering and Technology Tianjin University National Engineering Research Centre for Distillation Technology

The rheological properties of salt-tolerant partially hydrolyzed polyacrylamide(HPAM)solutions with molecular of 2.5×107 g/mol at different concentrations were measured in steady-state shear flow mode by Haake Rheostress 150 rheometer.Three constitutive equations(Oldroyd four constant model,Guesekus model and FENE-P model) were used for describing the apparent viscosity and first normal stress difference.The apparent viscosity of salt-tolerant HPAM solutions appears a first Newtonian zone when the shear rate is approximately lower than 0.2 s-1.At high shear rate,the HPAM solutions show shear-thinning and elasticity.The results show that the FENE-P model has the best agreement between theoretical and experimental data within the available shear rate range.The material parameters are useful for numerical analysis of polymer solution flow fields.

关键词： salt-tolerant partially hydrolyzed polyacrylamide steady-state flow shear thinning FENE-P model

Reduced Na^+ and K^+ permeability of K^+ channel in plasma membrane isolated from roots of salt-tolerant mutant of wheat

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Chinese Science Bulletin 1999年第9期44卷 816-821页

作者： Fangqing Guo Zhangcheng Tang Chinese Acad Sci Shanghai Inst Plant Physiol Shanghai 200032 Peoples R China

The Na^+ and K^+ permeability of K^+ channel in plasma membrane, isolated from roots of the salt-tolerant mutant of ,wheat, was lower than that of wild type in 100 mmol/L KCI and NaCI solution. The opening frequency of K^+ channel of the mutant reduced more significantly than that of wild type in two kinds of solution mentioned above. It is assumed that the reduction of opening frequency mainly contributes to the Na^+ and K^+ permeability of K^+ channel of the mutant. The electric conductance of single-channel of the mutant was similar to that of wild type and the main difference between them was exhibited as the opening frequency. Their K^+/Ka^+ selectivity of K^+ channel had no significant difference. The K^+/Na^+ selectivity of the mutant and wild type was 3.35 and 3. 18 respectively.

关键词： wheat salt-tolerant mutant plasma membrane salt adaptation.

Enhanced H^+ transport activity of tonoplast vesicles isolated from roots of salt-tolerant mutant of wheat under NaCl stress

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Chinese Science Bulletin 1999年第13期44卷 1198-1201页

作者： Fangqing Guo Zhangcheng Tang Chinese Acad Sci Shanghai Inst Plant Physiol Shanghai 200032 Peoples R China

Both enhanced were H^+ transport activities of tonoplast vesicles isolated from roots of the salt-tolerant mutant and wild type of wheat with treatment of NaCI, but the activity of the mutant was significantly higher than that of wild type. H^+ transport activity was indicated as the stable value of fluorescence quenching per mg membrane proteins. The H^+ transport activities dependent on ATP of the mutant and wild type were 1099 and 558 respectively and their activities dependent on PPi were 358 and 228 separately.

关键词： salt-tolerant mutant of wheat tonoplast proton transport NaCl stress.

Differential gene expression in salt-tolerant rice mutant and its parental variety

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Science China(Life Sciences) 1996年第3期39卷 310-319页

作者：张劲松周骏马张驰陈受宜 Plant Biotechnology Laboratory. Institute of Genetics Chinese Academy of Sciences Beijing 100101 China) Division of Biology Kansas State University Kansas USA.

The differential expressions of three genes rbcL, salt and rab!6 in response to ABA, NaCl, PEG and heat shock were investigated in seedlings of a salt-tolerant rice mutant 20 (mutant 20) and its parental variety Oryza sativa var. japonica 77-170(170). By Northern blot analysis it was found that ABA induced the expression of all three genes of rbcL, salt and rab16 in shoots and roots of both 170 and mutant 20 with the exceptions of rab16 in shoots of mutant 20 and rbcL in roots of 170. Lower concentrations of NaCl induced rbcL expression in shoots of mutant 20 but not 170. Higher concentrations of NaCl decreased rbcL expression but induced expressions of salt and rab16 in shoots of both 170 and mutant 20. PEG(15%) and 37℃ heat shock showed almost no effects on the expression of the three genes in mutant 20. However, they caused a decrease in rbcL expression and slight induction of the rab16 gene in 170, with salt expression unaffected. These results indicated that mutant 20 was relatively less

关键词： rice salt-tolerant mutant environmental stress differential expression of genes.

Analysis of genes specifically expressed under salt stress in salt-tolerant mutant of rice by using DDRT-PCR technique

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Science China(Life Sciences) 1996年第4期39卷 385-394页

作者：张弛陈受宜 Plant Biotechnology Laboratory Institute of Genetics Chinese Academy of Sciences Beijing 100101 China

Differential display reverse transcription-PCR (DDRT-PCR) technique was used to identify those genes that are expressed differentially between wild type rice variety 77-170 (Oryza Sativa vas Japonica) and its salt-tolerant mutant (M-20) under salt stress. Totally 13 salt-inducible cDNA fragments of 200-600 bp were identified and doned, and were designated as SIGR1 - SIGR13 (salt-induced gene in rice). Northern blot analysis showed that expression of SIGR6 and SIGR8 was salt-inducible in both wild type and mutant, and expression of SIGR12 in M-20 was much higher than that in 77-170 under salt stress. It was also shown that expression of SIGR3, SIGR4, SIGR7, SIGRIO and SIGR13 was salt-inducible, and the genes were highly homologous with Rab1d which was an ABA-inducible gene of rice. The great potential application of DDRT-PCR technique in plant molecular biology research may promote the investigation of expression of salt-induced protein in rice.

关键词： rice salt-tolerant mutants DDRT-PCR salt-induced gene expression.

Response of salt-tolerant Rice Varieties to Biocompost Application in Sodic Soil of Eastern Uttar Pradesh

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American Journal of Plant Sciences 2014年第1期5卷 7-13页

作者： Akhtar H. Khan Ashok K. Singh Mubeen Sudhanshu Singh Najam W. Zaidi Uma S. Singh Stephan M. Haefele 1Department of Crop Physiology Narendra Deva University of Agriculture and Technology Kumarganj India Australian Centre for Plant Functional Genomics University of Adelaide Adelaide Australia Department of Crop Physiology Narendra Deva University of Agriculture and Technology Kumarganj India International Rice Research Institute New Delhi India

Sodic soils have immense productivity potential, if managed through proper technology interventions. Biocompost is prepared by composting pressmud (a sugar industry byproduct) received from cane juice filtration and spent wash received from distilleries through microbial aerobic decomposition and can be used to reclaim sodic soils. Field experiments were conducted during the wet season of 2011 and 2012 to study the effect of incorporation of biocompost in sodic soil with four treatments: T1—Control, T2—Biocompost at 2 t ha-1, T3—Biocompost at 4 t ha-1 and T4—Biocompost at 6 t ha-1. The two promising salt tolerant rice varieties preferred by farmers, Narendra usar 3 and NDR 359 were used as test crops, which can produce yields ranging between 2-4 t ha-1 in soil having a pH range of 9.2 to 10.5. Among the different doses of biocompost tested, application of biocompost at 6 t ha-1 registered highest yields, enabled by a higher biomass, ear bearing tiller (EBT), and grain fertility in both varieties. Narendra usar 3 was more responsive to treatments even at lower doses of biocompost than NDR 359, but NDR 359 yielded slightly higher than Narendra usar 3 in all treatments. Soil health was also improved evidently on better fertility and low soil pH and EC at harvest. Thus, biocompost can be considered as a commercially viable, environmentally acceptable and practically enforceable option for improving the crop productivity and soil fertility status.

关键词： Biocompost Narendra usar 3 NDR 359 salt-tolerant Varieties Sodic Soil

QTL mapping by whole genome re-sequencing and analysis of candidate genes for salt tolerance in linseed(Linum usitatissmum L.)

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Oil Crop Science 2022年第2期7卷 80-85页

作者： Wei Zhao Yanping Zhang Jianping Zhang Yanni Qi Limin Wang Zhao Dang Yaping Xie Wenjuan Li Li Zhao Crop Institute Gansu Academy of Agricultural SciencesLanzhou730070China Gansu Provincial Academy of Agricultural Sciences Institute of Biotechnology Lanzhou730070China

Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield.However,the molecular mechanism of linseed response to salt stress is still unclear.In this study,a salt-tolerant(ST)linseed variety STS and a salt-sensitive(SS)variety DYM were selected as experiment materials.Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci(QTL).A total of 38,625 QTL loci were identified.Fifteen genes(which were not annotated in the reference genome)were identified within a 2.597 Mb region in chromosome 1.Two salt tolerance candidate genes Lus.***.m.scaffold91.141 and Lus.o.m.Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions.Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance.In this paper,results showed that Lus.***.m.scaffold91.141 and Lus.o.m.Scaffold1.14 might be involved in response to salt stress in lineseed.The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties.

关键词： Linseed salt-tolerant Whole-genome re-sequencing QTL Candidate genes

HAL1 mediate salt adaptation in Arabidopsis thaliana

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Cell Research 2001年第2期11卷 142-148页

作者： YANG SU XIN, YAN XIU ZHAO, QUAN ZHANG, YU KE HE, HUI ZHANG, DA LUO Institute of Plant Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, 300 Fenglin Road, Shanghai 200032, China Key Laboratory of Plant Stress Research, Sh Institute of Plant Physiology Shanghai Institutes for Biological Sciences Chinese Academyof Science Shanghai China Shanxi Normal University Xian China Key Laboratory of Plant Stress Research Shandong Normal University Jinan China

The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants’ genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K+ and Na+ showed that transgenic lines were able to retain less Na+ than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.

关键词： salt-tolerant Arabidopsis HAL1 gene engineering.

Potential of Novel Halotolerant and Psychrotrophic Strain of Planococcus sp. for Water Treatment

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Journal of Harbin Institute of Technology(New Series) 2018年第5期25卷 24-32页

作者： Kai Wang Siqi Li Xiquan Cheng Yingjie Zhang Peisheng Yan Jun Ma School of Marine Science and Technology Harbin Institute of Technology School of Municipal and Environmental Engineering Harbin Institute of Technology

To screen multifunctional bacteria for water treatment,a chemical oxygen demand( COD)degradation and flocculation strain S2 A15 was obtained from the offshore sewage outfall at Weihai International Beach. Based on the phylogenetic characteristics,a novel strain was identified as a Planococcus species. Strain S2 A15 was determined to have the ability of flocculation and COD degradation. A series of experiments showed that the strain S2 A15 could be used for the treatment of four types of wastewater,including domestic wastewater( 400 mg/L and 800 mg/L) and high salt domestic wastewater( 400 mg/L and 800 mg/L). Among them,the best effect was exerted by the strain that reduced by 76.9% in domestic wastewater with 400 mg/L COD. The flocculation ratio reached 60.19%. The optimal treatment conditions are also discussed. We confirmed that the strain S2 A15 had salt tolerance and low temperature resistance. The best growth of S2 A15 at salt concentration of 6% and further confirmed that the strain could degrade COD at a low temperature.

关键词： COD flocculation wastewater treatment Plauococcus sp. salt-tolerant bacteria