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Sodium Stress in the Halophyte Thellungiella halophila and Transcriptional Changes in a thsos1-RNA Interference Line

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Journal of Integrative Plant Biology 2007年第10期49卷 1484-1496页

作者： Dong-Ha Oh Qingqiu Gong Alex Ulanov Quan Zhang Youzhi Li Wenying Ma Dae-Jin Yun Ray A. Bressan Hans J. Bohnert Division of Applied Life Science (BK21 Program) and Environmental Biotechnology National Core Research Center Graduate School of Gyeongsang National University Jinju 660-701 Korea Departments of Plant Biology and of Crop Sciences University of Illinois at Urbana-Champaign Urbana IL 61801 USA Key Laboratory of Plant Stress Research College of Life Science Shandong Normal University Jinan 250014 China Key Laboratory of the Ministry of Education for Microbial and Plant Genetic Engineering Guangxi University Nanning 530005 China Department of Horticulture and Landscape Architecture Purdue University West-Lafayette IN 47907 USA

The plasma membrane Na＋/H＋-antiporter salt overly sensitive1 （SOS1） from the halophytic Arabidopsis-relative Thellungiella halophila （ThSOS1） shows conserved sequence and domain structure with the orthologous genes from Arabidopsis thaliana and other plants. When expression of ThSOSt was reduced by RNA interference （RNAi）, pronounced characteristics of salt-sensitivity were observed. We were interested in monitoring altered transcriptional responses between Thellungiella wild type and thsost-4, a representative RNAi line with particular emphasis on root responses to salt stress at 350 mmol/L NaCI, a concentration that is only moderately stressful for mature wild type plants. Transcript profiling revealed several functional categories of genes that were differently affected in wild-type and RNAi plants. Down-regulation of SOS1 resulted in different gene expression even in the absence of stress. The pattern of gene induction in the RNAi plant under salt stress was similar to that of glycophytic Arabidopsis rather than that of wild type Thellungiella. The RNAi plants failed to down-regulate functions that are normally reduced in wild type Thellungiella upon stress and did not up-regulate functions that characterize the Thellungiella salt stress response. Metabolite changes observed in wild type Thellungiella after salt stress were less pronounced or absent in RNAi plants. Transcript and metabolite behavior suggested SOS1 functions including but also extending its established function as a sodium transporter. The down-regulation of ThSOS1 converted the halophyte Thellungiella into a salt-sensitive plant.

关键词： salt stress salt overly sensitive1 RNA interference Thellungiella halophila transcript profiling.

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