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Elucidating the dynamics of polymer transport through nanopores using asymmetric salt concentrations

Nano Research 2022年第11期15卷 9943-9953页

作者： Martin Charron Lucas Philipp Liqun He Vincent Tabard-Cossa Department of Physics University of OttawaOttawaON K1N 6N5Canada

While notable progress has been made in recent years both experimentally and theoretically in understanding the highly complex dynamics of polymer capture and transport through nanopores,there remains significant disagreement between experimental observation and theoretical prediction that needs to be *** salt concentrations,where the concentrations of ions on each side of the membrane are different,can be used to enhance capture rates and prolong translocation times of electrophoretically driven polymers translocating through a nanopore from the low salt concentration reservoir,which are both attractive features for single-molecule ***,since asymmetric salt concentrations affect the electrophoretic pull inside and outside the pore differently,it also offers a useful control parameter to elucidate the otherwise inseparable physics of the capture and translocation *** this work,we attempt to paint a complete picture of the dynamics of polymer capture and translocation in both symmetric and asymmetric salt concentration conditions by reporting the dependence of multiple translocation metrics on voltage,polymer length,and salt concentration *** asymmetric salt concentration conditions,we experimentally observe the predictions of tension propagation theory,and infer the significant impact of the electric field outside the pore in capturing polymers and in altering polymer conformations prior to translocation.

关键词： nanopore DNA translocation salt gradient tension propagation polymer transport

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Effects of symmetric and asymmetric salt conditions on a selective solid-state nanopore assay

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Nano Research 2022年第11期15卷 9936-9942页

作者： Ian D.Wadsworth Adam R.Hall Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences Wake Forest School of MedicineWinston-SalemNC 27101USA Comprehensive Cancer Center Wake Forest School of MedicineWinston-SalemNC 27157USA

Conventional solid-state nanopore measurements sense all translocating entities,necessitating meticulous analysis to differentiate target *** address this,we have established a selective assay with the platform that has shown utility in quantifying several nucleic acid ***,limited detection efficiency and intrinsic noise have so far limited assay resolution to 10 *** in this value require manipulation of translocation ***,we report the effects of NaCl conditions on assay *** first investigate symmetric conditions,finding sensitivity increases with salt concentration but selectivity is maximized at 1.0 M *** then probe asymmetric conditions,showing a remarkable impact on assay sensitivity and selectivity when measurement buffer NaCl concentration in the reservoir with the translocating molecules is low and the opposite reservoir is *** optimum conditions,we demonstrate detection of target biomolecules down to a concentration of 100 pM which is an improvement of 2 orders of magnitude over past results.

关键词： solid-state nanopores biomarkers amplification-free detection salt gradient nucleic acids

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