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International Journal of Oral Science 2010年第1期2卷 35-44页

作者： Hao Li Xiao-lin Nong Qi Chen Yi-ping Yang Jia-quan Li Yan-ning Li Department of Oral and Maxillofacial Surgery College of Stomatology Guangxi Medical University Nanning China Department of Pathology Stomatological Hospital Affiliated to Guangxi Medical University Nanning China Laboratory Center Guangxi Medical University Nanning China Department of Epidemiology and Health Statistics College of Public Health Guangxi Medical University Nanning China

Aim To detect the expression of nerve growth factor （NGF） and vascular endothelial growth factor （VEGF） in salivary adenoid cystic carcinoma （SACC） tissues, as well as to determine the correlation between growth factor expression and prognosis in SACC. Methodology Medical records of 63 patients surgically treated for SACC between January 1988 and October 2005 were reviewed. Immunohistochemistry was performed to examine the expression of NGF and VEGF in tumor tissues. Kaplan-Meier analysis and Cox＇s proportional hazard regression model were applied to assess predictors of survival. Results NGF and VEGF were overexpressed in SACC tissues, compared with those in normal salivary tissues （P〈0.05）, and the staining intensity of these two factors was stronger in groups of solid subtype, advanced TNM stage, perineural invasion and recurrence. Patients with high- expression of NGF and VEGF, solid subtype, advanced stage, perineural invasion, recurrence and extended resection alone had worse survival rates （P〈0.05）. Conclusion NGF and VEGF are expressed increasingly in the tissues of SACC cases with invasion and metastasis. NGF expression and VEGF expression are independent prognosis factors for survival.

关键词： nerve growth factor vascular endothelial growth factor salivary adenoid cystic carcinoma prognosis

A20 inhibits human salivary adenoid cystic carcinoma cells invasion via blocking nuclear factor-kB activation

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Chinese Medical Journal 2007年第20期120卷 1830-1835页

作者： ZHANG Bin GUAN Cheng-chao CHEN Wan-tao ZHANG Ping YAN Ming SHI Jiu-hui QIN Chun-lin YANG Qian Department of Oral and Maxillofacial Surgery Second Affiliated Hospital Harbin Medical University Harbin 150001 China Department of Stomatology Harbin Medical University Harbin 150001 China Department of Oral and Maxillofacial Surgery Ninth People＇s Hospital College of Stomatology Shanghai Jiao Tong University Shanghai 200011 China Department of Biomedical Sciences Baylor College of Dentistry Texas A and M University System Health Science Center Dallas TX 75246 USA Department of Life Science and Engineering Harbin Institute of Technology Harbin 150001 China

Background A20, also known as tumor necrosis factor α induced protein 3 （TNFaip3）, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B （NF-κB） activity and prevents tumor necrosis factor （TNF）-mediated programmed cell death. NF-κB is a transcription factor that regulates expression of genes involved in cell proliferation, cell survival and anti-apoptosis. Several studies have implicated that the NF-κB signal pathway is associated with angiogenesis and clinico-pathological process of adenoid cystic carcinoma （ACC） of the salivary glands.Methods The ability of overexpression of A2.0 to influence the biological behavior and invasion of ACC cells was examined. The cells were stably transfected with full-length A20 cDNA. Stable gene transfer was verified by realtime-polymerase chain reaction （PCR） and Western blot analysis. The change of cell biological behavior was examined by methyl thiazolyl tetrazolium （MTT） and NF-κB luciferase reporter assay and the invasion of the cells was examined by a Matrigel invasion chamber.Results pEGPFN3-A2.0 gene was stably transferred into ACC-2 cells and overexpressed. When cells were treated with TNFα, the NF-κB activity of ACC-2-A20 cells could be down-regulated about 46.32% in contrast to ACC-2-GFP cells （P〈0.05）. A20 potently inhibited growth of A20 transfectant ACC-2-A20 compared with control vector transfected groups and the ACC-2 empty control group （P〈0.05）. The ACC-2-A20 cells showed significantly reduced ability to invade through Matdgei-coated filters compared to ACC-2-GFP and ACC-2 cells. The inhibition rate was up to 71.05% （P〈0.05）. Conclusions A2.0 gene transfer is associated with decreased tumor invasion, in part via the down-regulation of NF-κB expression, providing evidence for a potential application of A20 in designing a treatment modality for salivary gland cancers such as ACC.

关键词： A20 nuclear factor-κB invasion salivary adenoid cystic carcinoma

EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR IN DIFFERENT salivary adenoid cystic carcinoma CELL LINES

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Chinese Journal of Cancer Research 2005年第3期17卷 213-216页

作者：马杰宗志红王兆元 Department of Oral Medicine The Second Hospital China Medical University Laboratory of Biochemistry China Medical University Department of Pathology Huayu Hospital China Medical University

Objective： To investigate the expression of epidermal growth factor receptor, a receptor tyrosine protein kinase, in the subcellular fractions of human salivary adenoid cystic carcinoma cell lines SACC-83 and SACC-LM. Methods： Low metastatic and high metastatic cells of the adenoid cystic carcinoma, SACC-83 and SACC-LM, were cultured. Their subcellular fractions were extracted. The expression of epidermal growth factor receptor was detected with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. Results： The results of Western blot analysis indicated that, EGFR expression on the membrane of SACC-83 cells was significantly higher than that of SACC-LM cells, but its expression in cytoplasm was significantly less in the former than the later （P〈0.01）. In SACC-83 cell line, EGFR was over-expressed in membrane （P〈0.01）, but in SACC-LM cell line, EGFR was over-expressed in cytoplasm （P〈0.01）. Conclusion： The results suggest that the obtaining of metastasis ability is related to the high expression of EGFR protein in cytoplasm, so the molecular targeting therapy to EGFR may be an ideal treatment for the invasion and metastasis of salivary adenoid cystic carcinoma.

关键词： salivary adenoid cystic carcinoma Epidermal growth factor receptor Metastasis

ANTIMETASTATIC EFFECT OF INTEGRIN IIb/IIIa INHIBITORSON salivary adenoid cystic carcinoma

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Chinese Journal of Cancer Research 2001年第3期13卷 198-201页

作者：李风和俞光岩李盛琳彭师奇傅嘉 Peking University School of Stomatology Beijing Peking University School of Pharmacology Beijing

Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-highly metastatic SACC-83 and effect of aspirin, arginine-aspartate (RD), magnesium acetylsalicylate on adhesion were studiedin vitro. Antimetastafic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observedin vivo. Results: The tumor cell-platelet adhesion was stronger in SACC-LM than in SACC-83. Aspirin, RD and magnesium acetylsalicylate could inhibit the adhesion of SACC-LM at the concentration of 1, 5 and 25 μg/ml. RD can inhibit experimental metastasis of SACC. Conclusion: Metastasis of SACC is related to platelet-tumor cell adhesion, RD could inhibit metastasis of SACC.

关键词： salivary adenoid cystic carcinoma Integrin IIb/IIIa Platelet Neoplasm metastasis

Upregulation of lncRNA ADAMTS9-AS2 Promotes salivary adenoid cystic carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

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Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid...

2020年全国口腔颌面-头颈肿瘤学术大会暨中华口腔医学会口腔颌面-头颈肿瘤专业委员会学术年会——创新超越、共赢未来

作者：谢舒乐李博文方泽震黄国鑫张杜合一李劲松中山大学孙逸仙纪念医院

关键词： salivary adenoid cystic carcinoma lncRNA metastasis

Chloroquine enhances cisplatin sensitive by suppressing autophagy in human salivary adenoid cystic carcinoma

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Chloroquine enhances cisplatin sensitive by suppressing auto...

2020年全国口腔颌面-头颈肿瘤学术大会暨中华口腔医学...

作者： Bin Zhou 中山大学孙逸仙纪念医院

Purpose/Background : Autophagy was proved to be involved in tumor progression and chemo-radiotherapy resistance. Chloroquine(CQ), an autophagy inhibitor, can increase chemo-radiotherapy sensitivity in several cancer cells. However, the correlation between CQ and chemotherapy sensitive in salivary adenoid cystic carcinoma(ACC) remains obscure.

关键词： Chloroquine cisplatin sensitive salivary adenoid cystic carcinoma autophagy

Upregulation of lncRNA ADAMTS9-AS2 Promotes salivary adenoid cystic carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

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Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid...

2019第一届全国口腔颌面-头颈肿瘤学术大会——聚合引领、协同发展

作者：林钊宇李劲松中山大学孙逸仙纪念医院

Purpose/Background:Neurotropic infiltrative growth and distant metastasis are the main causes of death in salivary adenoid cystic carcinoma(SACC) patients. Long noncoding RNAs(lncRNAs) are involved in many human neoplasms, however, their potential roles in SACC are *** and Methods:We screen the expression profile of lncRNAs in two SACC cells(SACC-83 and SACC-LM) via RNA-sequencing. In situ hybridization and RT-qPCR were used to determine the level and prognostic values of ADAMTS9-AS2 in SACC tissues. Then, functional experiments in vitro and in vivo were performed to investigate the effects of ADAMTS9-AS2 on tumor growth, metastasis in SACC. Mechanistically, RT-qPCR, dual luciferase reporter assay RNA pull-down and RNA immunoprecipitation experiments were performed to confirm the interaction between ADAMTS9-AS2 and miR-143-3p in SACC cells.

关键词： salivary adenoid cystic carcinoma metastasis metastasis lncRNA lncRNA

circSMOC1 Promotes salivary adenoid cystic carcinoma Metastasis via miR-885-3p/NNMT axis

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circSMOC1 Promotes Salivary Adenoid Cystic Carcinoma Metasta...

2020年全国口腔颌面-头颈肿瘤学术大会暨中华口腔医学会口腔颌面-头颈肿瘤专业委员会学术年会——创新超越、共赢未来

作者：谢舒乐林钊宇李博文张杜合一李劲松中山大学孙逸仙纪念医院

Purpose/Background:Neurotropic infiltrative growth and distant metastasis are the main causes of death in salivary adenoid cystic carcinoma(SACC) *** RNAs(circRNAs) are involved in many human neoplasms, however, the p... 详细信息

关键词： salivary adenoid cystic carcinoma circRNA metastasis ceRNA

涎腺腺样囊性癌中蛋白磷酸酶2A-Aβ表达的研究

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涎腺腺样囊性癌中蛋白磷酸酶2A-Aβ表达的研究

第三次全国涎腺疾病学术会议

作者：徐中飞王洁王绪凯孙长伏中国医科大学口腔医学院口腔颌面外科中国医科大学口腔医学院口腔颌面外科中国医科大学口腔医学院口腔颌面外科中国医科大学口腔医学院口腔颌面外科

目的:探讨蛋白磷酸酶2A-Aβ（Protein Phosphatase2A,PP2A-Aβ）在涎腺腺样囊性癌中表达的意义方法:采用免疫组化SP法检测40例涎腺腺样囊性癌组织中蛋白磷酸酶2A-Aβ的表达。结果:PP2A-Aβ在正常涎腺组织中主要分布于涎腺导管上皮,腺泡... 详细信息

目的:探讨蛋白磷酸酶2A-Aβ（Protein Phosphatase2A,PP2A-Aβ）在涎腺腺样囊性癌中表达的意义方法:采用免疫组化SP法检测40例涎腺腺样囊性癌组织中蛋白磷酸酶2A-Aβ的表达。结果:PP2A-Aβ在正常涎腺组织中主要分布于涎腺导管上皮,腺泡上皮和肌上皮细胞中;在SACC 组织PP2A-Aβ10例（25％）呈正常胞浆表达,30例（75％）呈现胞浆表达阴性或出现胞膜和（或）胞核内异位表达,两者之间存在显著性差异（p＜0．05）。结论:PP2A-Aβ表达降低或异位表达可能与SACC的发生有关,但与SACC的病理分型、TNM及淋巴结转移未显示相关性。

关键词： salivary adenoid cystic carcinoma PP2A-Aβ immunohistochemistry

涎腺腺样囊性癌体外诱导肿瘤血管内皮细胞的生物学特性研究

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涎腺腺样囊性癌体外诱导肿瘤血管内皮细胞的生物学特性研究

2018年中华口腔医学会第十次全国口腔黏膜病学术大会暨第八次全国口腔中西医结合学术大会

作者：吴芳龙周红梅高庆红口腔疾病研究国家重点实验室四川大学华西口腔医院口腔黏膜病科四川大学华西口腔医院口腔颌面外科

目的:肿瘤血管内皮细胞(tumor-derived endothelial cells, TDECs)在多种恶性肿瘤中发挥重要作用,但在涎腺腺样囊性癌(salivaryadenoidcystic carcinoma, SACC)中尚未见相关报道。本研究将探究SACC相关TDECs的生物学特性。方法:(1)采用Transwell将人真皮微血管内皮细胞(human dermal microvascular endothelial cells, HDMECs)与SACC细胞(ACC-2和ACC-M)共培养,以TEM-8为标记物鉴定TDECs;(2)采用CCK-8实验、划痕实验、Factin染色等检测TDECs生物学特性的改变。结果:(1)体外Transwell共培养诱导的TDECs呈TEM-8阳性表达;(2)TDECs的细胞增殖能力、迁移能力增强,并均有统计学差异(P <0.05);(3)TDECs中F-actin细胞骨架免疫荧光染色的表达显著强于HDMECs。结论:SACC细胞体外共培养诱导的TDECs具有较为特异的生物学行为,具有成为SACC抗血管治疗新靶点的潜能。

关键词： Tumor-derived endothelial cells salivary adenoid cystic carcinoma Human dermal microvascular endothelial cells Biological behaviors Co-culture