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Morphophysiological Diversity and Haplotype Analysis of Saltol QTL Region in Diverse Rice Landraces for salinity tolerance

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Rice science 2023年第4期30卷 306-320,I0010-I0012页

作者： B.M.LOKESHKUMAR S.L.KRISHNAMURTHY Suman RATHOR Arvinder Singh WARRIACH N.M.VINAYKUMAR B.M.DUSHYANTHAKUMAR Parbodh Chander SHARMA Division of Crop Improvement Central Soil Salinity Research InstituteKarnal-132001HaryanaIndia Department of Biotechnology Kuvempu UniversityShankaraghattaShimvamogga-577451KarnatakaIndia Department of Genetics and Plant Breeding University of Agricultural and Horticultural ScienceShimvamogga-577204KarnatakaIndia

Rice is sensitive to salinity stress at both the seedling and reproductive stages.The present study used 145 rice genotypes comprising of 100 landraces and 45 advanced breeding lines collected from different regions of India.These genotypes were evaluated in hydroponics under control[electrical conductivity(ECe)~1.2 dS/m]and saline(ECe~10.0 dS/m)environments along with susceptible(IR29)and tolerant(FL478)checks.The stress susceptibility index for eight morphophysiological traits was estimated.Analysis of variance showed significant differences among the genotypes for all the parameters studied in control,stress and relative stress conditions.We identified 3 landraces(Kuttimanja,Tulasimog and IET-13713I)as tolerant and 14 lines as moderately tolerant to salt stress.Strong correlations in the morphological(root and shoot lengths)and physiological traits(shoot Na^(+),Ca^(2+)and Mg^(2+)contents,and Na^(+)/K^(+)ratio)were observed under all the conditions.The hierarchical cluster analysis grouped the genotypes into five clusters,among which cluster Ⅱ comprised salt-tolerant lines.Haplotyping of Saltol region using 11 simple sequence repeat markers on 17 saline tolerant and moderately tolerant lines was conducted.Markers AP3206F,RM10793 and RM3412b,located close to SKC1 gene(11.23‒12.55 Mb),displayed diverse allelic variations and they were not related to the FL478 type.In this region,tolerant lines like Kuttimanja,IET-13713I and Tulasimog have new alleles.As a result,these lines may be suitable candidates for novel genomic regions governing rice salinity tolerance.Salt-tolerance ability of Kuttimanja,Tulasimog and IET-13713I was validated in two years in three salinity stress environments.These promising lines can be used in breeding programs to broaden the genetic base of salinity tolerance in rice,and it may help to dissect key genomic regions responsible for salinity tolerance.

关键词： haplotype salinity tolerance rice Saltol diversity SKC1 gene

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Fine-mapping and characterisation of genes on barley(Hordeum vulgare)chromosome 2H for salinity stress tolerance during germination

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The Crop Journal 2022年第3期10卷 754-766页

作者： Edward Mwando Yong Han Tefera Angessa Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityMurdochWA 6150Australia Department of Primary Industries and Regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia

salinity causes a detrimental impact on plant growth,particularly when the stress occurs during germination and early development stages.Barley is one of the most salt-tolerant crops;previously we mapped two quantitative trait loci(QTL)for salinity tolerance during germination on the short arm of chromosome 2 H using a CM72/Gairdner doubled haploid(DH)population.Here,we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively,using two DH populations of CM72/Gairdner and Skiff/CM72,F_(2)and F;generations of CM72/Gairdner/;Spartacus CL,Two Receptorlike kinase 4(RLPK4)v1 or Receptor-like kinase(RLK)v3 could be the candidates for enhanced germination under salinity stress because of their upregulated expression in salt-tolerant variety CM72.Besides,several insertion/deletion polymorphisms were identified within the 3 rd exon of the genes between CM72 and Gairdner.The sequence variations resulted in shifted functional protein domains,which may be associated with differences in salinity tolerance.Two molecular markers were designed for selecting the locus with receptor-like protein kinase 4,and one was inside HORVU2 Hr1 G111760.1 or HORVU.MOREX.r3.2 HG0202810.1.The diagnostic markers will allow for pyramiding of 2 H locus in barley varieties and facilitate genetic improvement for saline soils.Further,validation of the genes to elucidate the mechanisms involved in enhancing salinity tolerance at germination and designing RLPK4 specific markers is proposed.For this publication,all the analysis was based on barley reference genome of2017(v1),and it was used throughout for consistence.However,the positions of the markers and genes identified were updated according to new genome(v3)for reference.

关键词： Barley Germination salinity tolerance Diagnostic markers Receptor-like protein kinase 4 Gene expression Sequence analysis

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Marine urease with higher thermostability, pH and salinity tolerance from marine sponge-derived Penicillium steckii S4-4

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Marine Life Science & Technology 2021年第1期3卷 77-84页

作者： Changrong Liu Yao Xiao Yilin Xiao Zhiyong Li Marine Biotechnology Laboratory State Key Laboratory of Microbial MetabolismSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China

Urease has a broad range of applications,however,the current studies on urease mainly focus on terrestrial plants or microbes.Thus,it is quite necessary to determine if marine-derived ureases have diferent characteristics from terrestrial origins since the fnding of ureases with superior performance is of industrial interest.In this study,the marine urease produced by Penicillium steckii S4-4 derived from marine sponge Siphonochalina sp.was investigated.This marine urease exhibited a maximum specifc activity of 1542.2 U mg protein−1.The molecular weight of the enzyme was 183 kDa and a single subunit of 47 kDa was detected,indicating that it was a tetramer.The N-terminal amino acid sequence of the urease was arranged as GPVLKKTKAAAV with greatest similarity to that from marine algae Ectocarpus siliculosus.This urease exhibited a K_(m) of 7.3 mmol L^(−1) and a V_(max) of 1.8 mmol urea min^(−1) mg protein^(−1).The optimum temperature,pH and salinity are 55℃,8.5 and 10%,respectively.This urease was stable and more than 80%of its maximum specifc activity was detected after incubating at 25–60℃for 30 min,pH 5.5–10.0 or 0–25%salinity for 6 h.Compared with the terrestrial urease from Jack bean,this marine urease shows higher thermostability,alkaline preference and salinity tolerance,which extends the potential application felds of urease to a great extent.

关键词： Marine urease Penicillium steckii Thermostability Alkali resistance salinity tolerance

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Comparing salinity tolerance in embryonic and larval development of two species of water strider, Aquarius paludum and Gerris latiabdominis （Hemiptera： Gerridae）

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Insect Science 2013年第4期20卷 524-530页

作者： Manabu Kishi Tetsuo Harada Kenji Fujisaki Laboratory of Insect Ecology Graduate School of Agriculture Kyoto University Laboratory of Environmental Physiology Faculty ofEducation Kochi University Kochi Japan

Water strider Aquarius paludum （Fabricius） is a cosmopolitan species colonizes mainly freshwater but occasionally brackish habitats throughout the Palearctic and Oriental regions. Water strider Gerris latiabdominis （Miyamoto） is a common species in Japan lives in temporary habitats as freshwater paddy fields. These two species often occur syntopically. We investigated differences in the developmental response to brackish water during embryonic and larval stages between the two species. Eggs were exposed to 0-1.8% NaC1 solutions within 24 h of oviposition. Larvae of G. latiabdominis were exposed to salinities of 0, 0.5%, and 0.9% from the first instar until adult emergence. Limits of NaC1 concentration for hatching were 1.3% and 1.0% for A. paludum and G. latiabdominis, respectively. The hatching rate of G. latiabdominis was lower than that ofA. paludum at salinities 〉0.9%. The period of embryonic development of G. latiabdominis was more prolonged than that ofA. paludum at a given salinity. Although the salinity tolerance of G. latiabdominis was lower than that ofA. paludum, our results suggest G. latiabdominis has the physiological capacity to expand into brackish waters. High and low salinity tolerances of A. paludum and G. latiabdominis, respectively, reflect the relatively wide range of habitat salinities utilized by A. paludum and the relatively restricted habitats preferred by G. latiabdominis. The high salinity tolerance ofA. paludum could be an important factor contributing to their cosmopolitan distribution because high tolerance to salinity means the possibility of them to be dispersed via ocean or sea to other continents and islands.

关键词： embryo larva salinity tolerance water strider

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Selection of Rice Genotypes for salinity tolerance Through Morpho-Biochemical Assessment

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Rice science 2014年第5期21卷 288-298页

作者： Md. Nasim ALI Bhaswati GHOSH Saikat GANTAIT Somsubhra CHAKRABORTY Department of Agricultural Biotechnology Faculty Centre for Integrated Rural Development and Management School of Agriculture and Rural Development Ramakrishna Mission Vivekananda UniversityRamakrishna Mission Ashrama NarendrapurKolkata 700103 India Department of Crop Science Faculty of Agriculture University Putra Malaysia43400 Serdang SelangorMalaysia Sasya Shyamala Krishi Vigyan Kendra Ramakrishna Mission Vivekananda UniversityArapanch Sonarpur Kolkata 700150 India

The present study reported the morpho-biochemical evaluation of 15 selected rice genotypes for salt tolerance at the seedling stage. Growth parameters including shoot length, root length, plant biomass, plant turgid weight, plant dry weight along with relative water content were measured after exposure to saline solution （with electrical conductivity value of 12 dS/m）. Genotypes, showing significant differential responses towards salinity in the fields, were assessed through 14 salinity-linked morpho-biochemical attributes, measured at 14 d after exposure of seedling in saline nutrient solution. Relative water content, chlorophyll a/b, peroxidase activity and plant biomass were identified as potential indicators of salt tolerance. Principal component analysis and successive Hierarchical clustering using Euclidean distance revealed that Talmugur, Gheus, Ghunsi, Langalmura, Sabitapalui, and Sholerpona were promising genotypes for further breeding programmes in rice. The maximum Euclidean distance was plotted between Thavallakanan and Talmugur （7.49）, followed by Thavallakanan and Langalmura （6.82）, indicating these combinations may be exploited as parental lines in hybridization programmes to develop salinity tolerant variety.

关键词： chlorophyll content electrical conductivity Oryza sativa L. peroxidase activity prolinecontent relative water content salinity tolerance sugar content superoxide dismutase

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salinity tolerance in Argentinean Population of <i>Bromus catharticus</i>. Variability and Direct and Indirect Effects on Seedling Characters

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American Journal of Plant Sciences 2020年第12期11卷 2043-2058页

作者： Monica Beatriz Aulicino Monica Beatriz Collado Hernan Barca Maria del Carmen Molina Instituto Fitotécnico de Santa Catalina Facultad de Ciencias Agrarias y Forestales Universidad Nacional de La Plata La Plata Argentina

The aim of this research was to evaluate the salinity tolerance in prairie grass populations at the seedling stage quantifying the variability and the influence of physiological traits related to it. salinity tolerance, in Bromus catharticus Vahl (prairie grass) populations collected in different environments of the Pampean Phytogeography region (Argentine) was evaluated at the seedling stage, using controlled condition of temperature and light. It was adopted a completely randomized design using 3 plots with three plants each one per population and two levels of treatment: 0 mM and 100 mM NaCl. Morphological, biomass and membrane stability root and shoot traits were studied. A factorial ANOVA with interaction was estimated. Then one way ANOVA for all seedling traits in both treatments allowed estimating variance components, coefficient of genotypic determination (CGD) and variation index (VI). Comparisons between populations were made using Tukey test (at 5% of probability). Phenotypic correlations among traits were calculated and then a path coefficient analysis separated direct and indirect effects at 100 and 0 mM NaCl. No significant interactions “Population × Treatment” were found for any character. The saline stress caused a pairing in the population means for the most traits. Coefficients of variation were mainly higher when the seedlings grew without stress (0 mM) because it allowed a greater potential genotypic expression. The absence of significant interactions denotes a good homeostatic capacity of the prairie grass facing that abiotic stress. Leaf length, shoot length and root dry matter were the variables with the largest direct and indirect effects. Our results showed an increase for them at salt and demonstrated intraspecific variation, possibly in relation with the origin sites. Plants under stress showed a marked resilience, in order to quickly restore the sa

关键词： Bromus catahrticus Prairie Grass salinity tolerance Population Genetic Variability Path Coefficient Analysis

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Physiological and proteomic analysis of salinity tolerance in germinating seeds from Brassica napus

Physiological and proteomic analysis of salinity tolerance i...

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生态文明建设中的植物学：现在与未来——中国植物学会第十...

作者： Tan Longyan Zhao Qi Qiu Lili Dai Shaojun Alkali Soil Natural Environmental Science Center Northeast Forestry University College of Life and Environmental Sciences Shanghai Normal University

Soil salinity is a major abiotic stress that has a serious influence on seed germination.Studying salinity tolerance mechanism during seed germination will provide valuable information for engineering plants to enhance salt tolerance.Oilseed from Brassica species is an important source of vegetable oil,and most of the Brassica species are moderately salt-tolerant.In the present study,the mature and germinating seeds of Brassica napus were investigated under NaCl(0 mM,100 mM,and 200 mM) treatments using physiological and proteomic approaches.The germination rate,biomass,membrane integrity,osmolyte contents,and the activities of antioxidant enzymes in seeds were measured.Using comparative proteomic technology,57 and 46 salt-responsive proteins were identified in mature and germinating seeds under salt conditions,respectively.These proteins were mainly involved in signaling,carbohydrate and energy metabolism,protein metabolism,stress and defense.Our proteomics results suggested both nuclear and mitochondria genes-encoded proteins played important roles in seed germination and salt tolerance.The energy-related metabolism(e.g.TCA cycle,glycolysis,and gluconeogenesis) were enhanced under NaCl stress,which could provide sufficient energy for salt tolerance.Besides,the abundances and activities of antioxidant enzymes were changed in germinating seeds under NaCl stress,implying that diverse ROS scavenging pathways were triggered to cope with salt stress.In addition,our results revealed that alterations in osmotic homoestasis,active protein processing and turnover,and enhanced methionine cycle were necessary for seed germination and salt tolerance.

关键词： Seed germination Brassica napus Proteomics salinity tolerance

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Diversity of Sodium Transporter HKT1;5 in Genus Oryza

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Rice science 2022年第1期29卷 31-46,I0002-I0017页

作者： Shalini PULIPATI Suji SOMASUNDARAM Nitika RANA Kavitha KUMARESAN Mohamed SHAFI Peter CIVÁŇ Gothandapani SELLAMUTHU Deepa JAGANATHAN Prasanna Venkatesan RAMARAVI S.PUNITHA Kalaimani RAJU Shrikant S.MANTRI R.SOWDHAMINI Ajay PARIDA Gayatri VENKATARAMAN M.S.Swaminathan Research Foundation Chennai 600113India National Agri-Food Biotechnology Institute Punjab 140306India Krishi Vigyan Kendra Kanyakumari 629901India National Centre for Biological Sciences/Tata Institute of Fundamental Research Bangalore 560065India National Research Institute for Agriculture Food and Environment UniversityClermont-AuvergneClermont-Ferrand 63000France Forest Molecular Entomology Laboratory Faculty of Forestry and Wood SciencesCzech University of Life SciencesPraguePraha 16500Czech Republic Tamil Nadu Agricultural University Coimbatore 641003India International Business Machines India Chennai 600125India Institute of Life Sciences Bhubaneswar 751023India

Asian cultivated rice shows allelic variation in sodium transporter,OsHKT1;5,correlating with shoot sodium exclusion(salinity tolerance).These changes map to intra/extracellularly-oriented loops that occur between four transmembrane-P loop-transmembrane(MPM)motifs in OsHKT1;5.HKT1;5 sequences from more recently evolved Oryza species(O.sativa/O.officinalis complex species)contain two expansions that involve two intracellularly oriented loops/helical regions between MPM domains,potentially governing transport characteristics,while more ancestral HKT1;5 sequences have shorter intracellular loops.We compared homology models for homoeologous OcHKT 1;5-K and OcHKT1;5-L from halophytic O.coarctata to identify complementary amino acid residues in OcHKT1;5-L that potentially enhance affinity for Na+.Using haplotyping,we showed that Asian cultivated rice accessions only have a fraction of HKT1;5 diversity available in progenitor wild rice species(O.nivara and O.rufipogon).Progenitor HKT1;5 haplotypes can thus be used as novel potential donors for enhancing cultivated rice salinity tolerance.Within Asian rice accessions,10 non-synonymous HKT1;5 haplotypic groups occur.More HKT1;5 haplotypic diversities occur in cultivated indica gene pool compared to japonica.Predominant Haplotypes 2 and 10 occur in mutually exclusive japonica and indica groups,corresponding to haplotypes in O.sativa salt-sensitive and salt-tolerant landraces,respectively.This distinct haplotype partitioning may have originated in separate ancestral gene pools of indica and japonica,or from different haplotypes selected during domestication.Predominance of specific HKT1;5 haplotypes within the 3000 rice dataset may relate to eco-physiological fitness in specific geo-climatic and/or edaphic contexts.

关键词： HKT1 5 diversity single nucleotide polymorphism haplotype bacterial artificial chromosome salinity tolerance sodium transporter Oryza species

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Applications of ambient electric arc ionization mass spectrometry in saline samples

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Chinese Chemical Letters 2022年第5期33卷 2708-2710页

作者： Yuan Li Yuanji Gao Binpeng Zhan Weiwei Chen Fengjian Chu Hongru Feng Zhan Gao Zihan Ma Yuanjiang Pan Department of Chemistry Zhejiang UniversityHangzhou 310027China College of Chemistry and Materials Science Sichuan Normal UniversityChengdu 610068China

salinity tolerance of ambient electric arc ionization(AEAI)was evaluated by comparing electrospray ionization for various samples at NaCl concentrations from 0 to 1000 mmol/L.AEAI-mass spectrometry(AEAI-MS)exhibited an excellent signal intensity even at NaCl concentrations of 1000 mmol/L,while the ESI-MS had no signal because high salinity has a strong inhibitory effect on analytes.The sodium adduct was verified using LiCl instead of NaCl.AEAI-MS successfully quantified saline samples with an excellent quantitative ability(R^(2)≥0.998).We also achieved some analytical samples in the buffer solution at a very high concentration and even in a saturated salt solution.Overall,AEAI-MS has protonated ions for most target analytes.In addition,the relationship between auxiliary temperature and the distance from the sample to the arc was investigated,and the results indicated that thermal desorption plays an important role in AEAI source.

关键词： Electric arc Ion source salinity tolerance Mass spectrometry Ambient electric arc ionization

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Early ABA-stimulated maintenance of Cl^(−) homeostasis by mepiquat chloride priming confers salt tolerance in cotton seeds

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The Crop Journal 2021年第2期9卷 387-399页

作者： Ning Wang Xiangru Wang Hengheng Zhang Xiaohong Liu Jianbin Shi Qiang Dong Qinghua Xu Huiping Gui Meizhen Song Gentu Yan State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina

Mepiquat chloride(MC)priming alleviates the effects of salt stress during seed germination in cotton(Gossypium hirsutum L.),but the mechanisms underlying its effects are unknown.We found that MC priming increases salt tolerance,as evidenced by marked increases in seed vigor and germination rates,and alleviated salt toxicity by reducing Cl^(−)accumulation in germinating seeds.Consistently,electrophysiological experiments revealed that the seeds with MC priming displayed superior Cl^(−)exclusion ability in the root apex.These beneficial effects of MC priming were abolished by the abscisic acid(ABA)-synthesis blocker fluridone under salt stress.MC priming induced an early response to acclimatization and stress,as indicated by rapidly increasing ABA content during initial exposure to salt stress.Transcriptome analyses revealed that MC priming induced an array of differentially expressed genes(DEGs)in germinating seeds.The most noticeable changes in germinating seeds were MC priming-induced increases in the expression of DEGs encoding components of ABA biosynthesis,ABA catabolism,and ABA signaling pathways under salt stress.MC priming also increased the expression of some DEGs encoding Cl^(−)ion transporters(e.g.CCC,SLAC1/SLAH1/SLAH3,CLC,and ALMT9)in germinating seeds.These results indicate that MC priming-induced ABA contributes to Cl^(−)homeostasis in tissues and acts as a positive regulator of salt tolerance via regulation of Cl^(−)transporters(particularly CCC and SLAC1/SLAH1/SLAH3).Taken together,these findings shed light on the molecular mechanism underlying MC-mediated tolerance to salt stress during seed germination.

关键词： Seed priming salinity tolerance Ionic flux RNA-Seq ABA

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